GENES

DEVELOPMENT

IN UPCOMING ISSUES . . . .

The lin-14 locus of Caenorhabditis elegans controls the time of expression of specific postembryonic development events

Victor Ambros and H. Robert Horvitz

Nucleic acid splicing events occur frequently during macronuclear development in the protozoan Oxytricha nova and involve the elimination of unique DNA

Rosa Maria Ribas-Aparicio, Jason J. Sparkowski, Anne E. Proulx, John D. Mitchell, and Lawrence A. Klobutcher

Ultrabithorax mutations in common and variable regions of the protein coding sequence

Robert Weinzierl, J. Myles Axton, Alain Ghysen, and Michael Akarn

Retroviral transfer and expression of the IL-3 gene in hemopoietic cells

Peter M.C. Wong, Siu-Wah Chung, and Arthur W. Nienhuis

Deletion and duplication of DNA sequences is associated with the embryonic lethal phenotype of the t 9 complementation group of the mouse t complex

Maja Bucan, Bernhard G. Herrmann, Anna-Maria Frischauf, Victoria L. Bautch, Vernon Bode, Lee M. Silver, Gail R. Martin, and Hans Lehrach

Activation and repression of mammalian gene expression by the c-myc protein

Rima Kaddurah-Daouk, John M. Green, Albert S. Baldwin, Jr., and Robert E. Kingston

r

r

I - I I IIII I

Q COLD SPRING HARBOR

LABORATORY CONFERENCE ON

YEAST CELL BIOLOGY August 11-August 16

Organized by Amar Klar, Cold Spring Harbor Laboratory

Paul Nurse, Imperial Cancer Research Fund Randy Schekman, University of California, Berkeley

A specialized international Yeast meeting emphasiz- ing areas of cell cycle controls, developmental choices, cell- cell recognition, cytoskeleton and cell structure, protein targeting and modification, elements of chro- mosome structure and function, and other relevant areas will be held from August 11-16, 1987. Open call for abstracts. The abstract deadline is JUNE 2. For further information, contact:

Meetings Coordinator Cold Spring Harbor Laboratory P.O. Box 100 Cold Spring Harbor, NY 11724 (516) 367.8346

Q COLD SPRING HARBOR LABORATORY announces the sixth annual cancer cells

conference on EUKARYOTIC DNA REPLICATION

September 2-6, 1987 Organized by:

T.J. Kelly, Johns Hopkins University B. Stillman, Cold Spring Harbor Laborator'y

Topics will include: Replication of Virus DNA Replication of Extra-Chromosomal Elements Replication and Amplification of Chromosomal DNA Control of Cell Cycle and S phase Replication Proteins Chromosome Structure and Segregation A special session on Prokaryotic Model Systems The meeting will include formal discussion sessions and poster sessions. The organizers invite submis- s/on .of abstracts by June 24, 1987. Requests for registration and/or abstract materials should be forwarded to:

• Meetings Coordinator Cold Spring Harbor Laboratory P.O. Box 100 Cold Spring Harbor, NY 11724 (516) 367-8346

J

¢

¢1 ",v " " b ~,O • ,¢ ,,~O

(t~" (RESEAR_CHE_R S )Harva rd

Co,n ZZ \ i S o.

For p t p e t t e t t p s and o t h e r p l a s t i c d isposab les , a t p r i c e s t h a t are b e t t e r - t h a n - c o m p e t i t i v e :

NQRTECH LABORATORIES 4 NtdZand Avenue H i c k s v t l Z e , NY 11801 (516) 935 -2040

r

Q Conference on

TRANSLATIONAL CONTROL September 16-20, 1987

Organized by: Dr. John W.B. Hershey, University of California at Davis Dr. Michael B. Mathews, Cold Spring Harbor Laboratory

Dr. Brian Safer, National/nstitutes of Health

Topics for discussion will include: • mRNA structure and recognition • translation of specific mRNAs • stress phenomena (heat shock, etc.) • viral systems • differentiation, development and growth control • regulation of elongation and termination • genes for translational components and their regulation • protein secretion and processing

Supported by a grant from/CN Biomedica/s, /nc. Registration and abstract materials are available from:

Meetings Coordinator COLD SPRING HARBOR LABORATORY

P.O. Box 100 Cold Spring Harbor, N.Y. 11724

(516) 367-8345

,iiiiiiii!!i~ii~

iiiiiiiiii::,

Until now, when it came to preparative DNA electrophoresis, researchers turn- ed to 366nm Iongwave transilluminators to reduce photodamage, but sacrificed sensitivity. Today there's a solution.

FOTODYNE presents FOTO/PREP I. The first innovative transilluminator to offer the sensitiv- ity of 30Onto midrange UV, with the kind of pro- tection previously provided only by 366nm UV sources.

The key? A unique sensitivity control device. In the analytical mode it provides the nanogram level sensitivity distinguishing all FOTODYNE 300nm DNA transilluminators. Switch to the preparative mode, and a 2000bp band containing less than 10 nanograms of DNA can be visualized for cutting.

r 7 . , . ~ - - - = . . . : .

More importantly, the gel can be exposed as long as 20 minutes with little or no detectable photonicking damage!

FOTO/PREP I comes with a replaceable UV transparent protective sheet, so gels can be cut directly on its surface without damaging the UV glass. The molded, UV blocking cover can be raised from 0 ° to 90 ° to allow easy access to the gel

FOTO/PREP I. Available today, only from FOTODYNE. The leader you rely on for innovative advancements in DNA analysis instrumentation.

For more information call or write us. And ask for our free new catalog.

III II C a l l 1 - 8 0 0 - D N A - F O T O FOTODYNE, INC. ~ 414-786-9550 16700 W. Victor Road• or New Berlin, Wisconsin 53151-4131 U.S.A. TELEX 260127

See FOTO/PREP I at ASBC, Booth 504-506, June 8-11, 1987.

FOTODYNE I N C O R P O R A T E D

GENETIC MANIPULATION OF THE EARLY MAMMALIAN EMBRYO

Banbury Report 20 Edited by Frank Costantini, Columbia University; Rudolf Jaenisch, Whitehead Institute and Department of Biology, Massachusetts Institute of Technology

Gene transfer techniques have brought a new power to bear upon fun- damental questions of mammaiian gene regulation and function during develop- ment. The fall 1984 Banbury conference on the genetic manipulation of the mammalian ovum and early embryo assembled major elements in such ap- proaches. Prominent among these approaches was the introduction of cloned genes directly into the mammalian germline, the introduc- tion of cells into early embryos, and the use of developmental muta- tions for identifying and isolating the specific genes affected. This volume contains the papers presented at that conference. As a col- lection of key strands of research in this area, this book should be particularly useful in gauging present capabilities as well as for gaining a perspective on how this rapidly moving new field is likely to develop in the near future.

1985, 289 pp., illus., indexes Cloth S63

I

LC 85-13233 CIP ISBN 0-87969-220-0

MANIPULATING THE MOUSE EMBRYO

A Laboratory Manual By

B.L.M. Hogan, National Institute for Medical Research

F. Costantini, Columbia University

E. Lacy, Memorial SIoan-Kettering Cancer Center

Q Cold Spring Harbor Laboratory P.O. Box 100 Cold Spring Harbor, New York 11724

Originally developed as a laboratory manual for the Molecular Embryol- ogy of the Mouse course taught at Cold Spring Harbor Laboratory, MANIPULATING THE MOUSE EMBRYO has been expanded to provide a detailed and up-to-date compendium of the techniques involved in the study of early mouse development and in the genetic manipulation of the mouse em- bryo. Beginning with a history of the mouse as an experimental animal for the study of the genetics of mammalian development and a review of current knowledge on early mouse development at the cellular and molecular levels, the book then moves on to describe isolation of pre- and postimplantation em- bryos, embryo culture, embryo transfer and other experimental procedures on mice, microinjection of DNA into fertilized mouse eggs, nuclear transplanta- tion, isolation and culture of embryonic cell lines, and chimera formation by injection of stem cells into the blastocyst. Because it assumes no prior ex- perience in the handling of the mouse embryo, this amply illustrated manual will be useful to scientists who wish to work with these materials for the first time and to students in graduate-level courses in developmental biology, as well as to experienced embryologists interested in exploring and applying new techniques such as gene transfer by DNA microinjection.

, ,

1986, 332 pp., LC 84-17628 CIP illus., colorplates, appendix, bibliography, index ISBN 0-87969-175-1 Paper $60

Cold Spring Harbor Symposia Molecular Biology

Thirteen years marked the time between the discovery of the double helix in 1953 and the elucidation of the genetic code in 1966. A similar interval has now passed since the development by Cohen and Boyer of a simple procedure for the cloning of selective DNA fragments. The scientific advances made possible by the subsequent modification and elaboration of these original cloning procedures are increasingly overwhelming. Facts that until recently were virtually unobtainable now flow forth almost effortlessly. Most excitingly, the frenetic pace of these new discoveries, instead of marking the impending end of a glorious moment of learning, give every indication of opening up scientific frontiers that will take many years to explore thoroughly.

This new era of enlightenment is nowhere more apparent than in the newfound ability to study man at the molecular level. By focusing on the molecular biology of Homo sapiens as the topic for its 51st Symposium, Cold Spring Harbor Laboratory chose a topic that most certainly will be returned to over and over during the second 50

y e a r s ofi ts Symposium.

CONTENTS

INTRODUCTION (W.F. Bodmer)

HUMAN GENE MAP Summaries and Recent Additions The human gene map (V.A. McKusick); Linkage approaches to gene localization (R. White et al.); Analytical strategies for genetic mapping (J.-M. Lalouel et al.); Mapping complex genetic traits (E. Lander, D. Botstein); Human MHC (J.L. Strominger); HLA class-II genes (B. Mach et al.); Molecular biology of the MHC (J.I. Bell et al.); Class II RFLPs (S.W. Serjeantson et al.); Human DNA repak gene ERCC-1 (J.H.J. Hoeijmakers et al.); Human mtDNA-encoded NADH dehydrogenase subunits (G. Attardi et al.)

New Mapping Strategies Physical mapping methods (C.L. Smith, C.R. Cantor); Mapping and cloning megabase DNA (S. K. Lawrance et al.); Molecular ap- proaches to mammalian genetics (A. Poustka et al.); Flow cyto- genetics (J.W. Gray et al.); Fluorescence hybridization (D. Pinkel et al.); Chromosome-specific DNA libraries (L.L. Deaven et al.); Flow-sorting analysis of the human genome (R.V. Lebo et al.); Cloning the X-CGD gene by linkage (B. Royer-Pokora et al); Nondisjunction in Trisomy 21 (S.E. Antonarakis et al.)

Recombination along Sex Chromosomes Genetic recombination and disease (M. Siniscalco); Genetic map- ping of the X chromosome (J.L. Mandel et al.); Molecular genetics of MIC2 (S.M. Darling et al.); Human telomeres (H.J. Cooke, B.A. Smith); Human X/Y pseudoautosomal region (F. Rouyer et al.); Sex reversal and the Y chromosome (D.C. Page); Molecular biology and pathology of the human Y chromosome (E. Seboun et al.); 46,XX and 45,X males (A. de la Chapelle)

GENETIC DIAGNOSIS Development of New Methodologies Analysis of DNA sequence variants in man (R.B. Wallace et al.); Polymerase chain reaction (K. Mullis et al.); Detection of single, base mutations in human DNA (R.M. Myers, T. Maniatis); Search- ing for gene defects (L.S. Lerman et al.); Molecular probes of chromosome aberrations (S.A. Lattet al.)

Applications: Cystic Fibrosis, Muscular Dystrophy, Huntington's Disease, Hemophilia A, Down's Syndrome, PKU, and Heart Disease Cystic fibrosis: The basic defect (R. Williamson et al.); RFLP probes as diagnostic tools (H. Donis-Keller et al.); Cystic fibrosis map- ping (L.-C. Tsui et al.); X-linked disease (K.E. Davies et al.); Translocations and muscular dystrophy (R.G. Worton et al.); Genetics of DMD (L.M. Kunkel et al.); Gene analysis of DMD (P.L. Pearson et al.); Huntington's disease (.J.F. Gusella et al.); Cloned factor VIII (R.M. Lawn et al.); Deficiency alleles of 3-globin and factor VIII:C genes (H.H. Kazazian, Jr. et al.); Overexpression of transfected h-CuZnSOD gene and DS (Y. Groner et al.); Carrier screening for PKU and somatic gene therapy (S.L.C. Woo et al.); Molecular biology of coronary arteriosclerosis (S. Deeb et al.)

HUMAN EVOLUTION Human genetic variation (L.L. Cavalli-Sforza et al.); Fossil evidence (P. Andrews); Evolution of Ig genes in primates (S. Ueda et al.); Rate of human mtDNA evolution (M. Stoneldng et al.); Paleomolecular biology of human remains (S. P/iiibo); Human protein sequences (R.F. Doolittle et al.); LINE-1 family of genes and pseudogenes (J. Skowronski, M.F. Singer); L-1 and reverse transcriptase (Y. Sakaki et al.); I. A/u evolution II. Human transposon (I. Sawada et al.); The human genome (G. Bemardi, G. Bernardi); c,-Thalassemia and the malaria hypothesis (A.V.S. Hill); Primate (J. Marks et al.)

DRUGS MADE OFF HUMAN GENES Clotting, Anti-dotting Factors Molecular assembly of plasma proteins (E.W. Davie et al.); yon Willebrand factor (J.E. Sadler et al.); Evolution of vitamin-K- dependent coagulation factors (G.L Long); Human factor VII cDNA isolation and expression (K. Berkner et al.); Structure- function relationships in human factor VIII (J.J. Toole et al.); Recombinant t-PA (G.A. Vehar et al.); Properties of scu-PA (D.C. Stump et al.)

Anti-cancer Agents Lymphoblastoid interferon production (N.B. Finter et al.); The interleukin-2 system (T. Taniguchi et al.); Lymphokines and monokines in anticancer therapy (W. Fiefs et al.); Tumor necrosis factors (D.V. Goeddel et al.); Tandem of TNF / cachectin and lym- photoxin genes (S.A. Nedospasov et al.); Cachectin: Dark side of TNF (A. Cerami, B. Beutler); Interleukin-1 (P.T. Lomedico et all); Mullerian-inhibiting substance (R.L. Care et al.)

II III

Building the future ®

Ultrafi l t rat ion technology is rap id ly tions and the lOmL size is ideal for replacing microf i l t ra t ion in m a n y exacting procedures , such as the concentra t ion of proteins and ma- cromolecules or the separa t ion of cells f rom fermenta t ion broth.

Ultraf i l t rat ion products manufac- tured by F ih ron Corporat ion, and avai lable exclusively from Fisher 's new Biotechnology Divisic, n, repre- sent s tate-of-the-art l abora tory technology.

• OMEGA TM and NOVA TM series ul- trafiltration membranes deliver constant , rel iable pe r fo rmance wi th accura te molecular cutoff points ranging from 1K to 300K NMWL. OMEGA m e m b r a n e s offer super ior low protein binding propert ies while also providing all the benefits you've come to expect from NOVA UF m e m b r a n e s - - s tabi l i ty agains t biological degrada t ion and most or- ganic solvents, reusabi l i ty , and long shelf life.

• NOVACELL T~' disposable stirred cell sys tems for small volume filtra- tion vir tual ly e l iminate handl ing problems.The new '150mL NOVA- CELL allows for cont inuous filtra-

IOI, Z

Fisher Scientific

smal ler procedures. These inexpen- sive cells are avai lable wi th ei ther OMEGA or NOVA membranes , ul- t rasonical ly sealed into the reser- voir units.

• NOVASETTE TM high-volume, con- tinuous tangential flow UF system allows you to process volumes from one liter to hundreds of liters in a ma t t e r of minutes. The new smal ler version MINISETTE TM is ideal when lab space is l imited or for smal ler batch UF operat ions. On both sys- tems all inlet and outlet ports are located logically on the bot tom manifold so you can add or change filter cassettes wi thout disconnect- ing any tubing. These units use filter cassettes containing OMEGA or NOVA membranes .

For more information about these fine UF products, or to learn more about our new Biotechnology Divi- sion, please use the reply card; wri te to us at 711 Forbes Avenue, Pi t tsburgh, PA 15219; or call 412- 562-8543.

Biotechnology Division

ii~ ~,,~ ~ii~,~ ~ ~iii ~ ~ ~ ~ ~i!!~ (i~ii!~i ~'~! iiiili ii~i ,,~" iii!

=

i11 Hi gher capacity..,plus~

Versati le in use.

• ,~ , ,~ f . . . . . . :~.~,,, -,.. ,:~.

- .., ~ o = ~ ~ ~*"~ ~

The Eppend Centri:fuge ~s safety, It's so accidentatt'v w O [ l t c a u s e

of motor dar~

~ a n c e r ~;ellS PAPILLOMAVIRUSES Edited by Bettie Steinberg and Janet Brandsma, Long Island Jewish Medical Center Lorne Taichman, State University of New York, Stony Brook

Studies on the papi l lomaviruses have expanded markedly over the past five years due to the availabi l i ty of recombinant reagents and in vitro systems and to the recognit ion of the strong associat ion of specif ic papi l lomaviruses with cer- tain carc inomas in humans. This book addresses molecular aspects of the virus in both transforma- tion and replication and also discusses several features of the molecular epidemiology of the human papi l lomaviruses.

CONTENTS Introduction (P.M. Howley)

Transcription BPV-1 E2 trans-activation (B.A. Spalholz et al.); BPV-1 en- codes a transcriptional repressor activity (P.F. Lambert et al.); HPV-18 transcription (F. Thierry et al.); HPV-8 en- hancer and BPV-1 E2 (R. Seeberger et al.); trans-Activation of a BPV early gene promoter (T.H. Haugen et al.); HPV-18 in cell lines and human cell hybrids (E. Schwarz et al.); HPV gene expression (L.T. Chow et al.); RNA probes of HPV transcription in respiratory papillomata (P. Ward et al.)

Papll lomavlrus-assoclated Protelns DNA-binding activity of BPV E2 (E.J. Androphy et al.); E5 polypeptide (R. Schlegel, M. Wade-Glass); Characteriza- tion of papillomavirus proteins (R.G. MaJIon et al.); HPV synthetic peptides (J.M. Palefsky et al.); HPV-6b and HPV-16 antibodies (J.M. Firzlaff et al.); HPV-1 E4 proteins in warts (F. Breitburd et al.); Expression of HPV, la late ORFs (J. Campione-Piccardo et al.); Provoked late gene expression (N.A. Jensen et al.); Papillomavirus-specific protein inductions (R.M. Levenson et al.); E6-E7 structure (O. Danos, M. Yaniv); Transforming-agent-specific secret- ed proteins (U.G. Brinckmann et al.) Repllcatlon Underreplication of HPV-1 DNA (S.S. Reilly, L.B. Taich- man); HPV-1 infection of cultured respiratory cells (C.B. Christian et al.); Transfection of keratinocytes (A. Farr et al.); HPV amplification (C.R. Brandt et al.) Genetics of Transformation Mutational analysis of BPV-1 (D. DiMaio et al.); Transfor- mation with HPV type-16 DNA (G. Matlashewski et al.); Transformation by HPV-16 and HPV-18 (L.A. Laimins et al.); Transforming gene of BPV-1 (P. Bergman et al.)

Transformation In Vltro and In Vlvo HPV-11 expression (J.W. Kreider et al.); BPV-l-transformed rodent fibroblasts (B. Bin~truy et al.); Progression of CRPV +l i , - . ~ r ~ l ~ ( ~ , - , h ~ ; t - 4 ~ r _ k A ~ * i ~ r ~ i . r~ . ~ ÷ ,'~1 ~" L I D ~ I ; ~ ,-,~11 ,-,~ , i t , , r ~

(S.L. Watts et al.); Cellular origin of condylomata acuminata (J. Buscema et al.); HPV transformation (P. Kaur, J.K. McDougall); Transformation induced by HPV-16 DNA (J.A. DiPaolo et al.); Interaction of papillomaviruses and car- cinogens (E. Amtmann et al.); BPV transformation and cellular responses (K.T. Smith et al.)

Relatlonshlp of Papil lomaviruses to Human and Animal Diseases HPV and cervical cancer (L. Gissmann et al.); Biological potential of cervical HPV infections (K. Syrj~nen et al.); Analysis of malignant tissues for HPV DNA (R.S. Ostrow et al.); Papillomavirus DNA in cervical tumors (P.G. Fuchs et al.); Papillomavirus infection of the nose (J. Brandsma et al.); Human cutaneous papillomas (S. Jablonska et al.); HPV type-specific markers in cervical lesions (P. Mose Larsen et al.); Comparison of in situ DNA hybridizations (S. Syrj~nen et al.); Transcription of HPV-16 in genital pre- cancers (G. Nuovo et al.); HPV and c-myc in cervical can- cer (P. Gariglio et al.); HPV integration in dysplasia (K. Shimoda, W.D. Lancaster); Homozygous integration of pap- illomavirus (P.A. Lazo); Viral tumors in inbred rabbits (A. Seto et al.); Mouse papillomavirus (J.P. Sundberg et al.) Immunology and Therapy NCMC in HPV-induced anogenital lesions (J. Malejczyk et al.); Anti-BPV immunity in patients with HPV infections (B.K. Beiss et al.); Therapies for HPV diseases (P.K. Weck, J.K. Whisnant); Interferon response and HPV (B.M. Steinberg et al.); Hematoporphyrin photodynamic ther- apy and CRPV (M.J. Shikowitz et al.)

June 1987, 480 pp. (approx.), lllus., Indexes LC 87-8030 Paper $80 ISBN 0-87969-301-0

ALSO AVAILABLE • Cancer Cells 4/DNA TUMOR VIRUSES:

Control of Gene Expresslon and Repllcatlon Edited by Michael Botchan, University of California; Terri Grodzicker, Cold Spring Harbor Laboratory; Philip Sharp, Massachusetts Institute of Technology 1986, 620 pp., lllus., Indexes LC 86-50649 CIP Paper $75 ISBN 0-87969-192-1

• Cancer Cells 3 GROWTH FACTORS AND TRANSFORMATION Edited by James Feramisco, Cold Spring Harbor Labora- tory; Brad Ozanne, University of Texas Health Science Center; Charles Stiles, Dana-Farber Cancer Institute. 1985, 450 pp., Illus., Indexes LC 85-3733 CIP Paper $70 ISBN 047969.178-6

• Cancer Cells 2/ONCOGENES AND VIRAL GENES Out of print

• Cancer Cells 1/THE TRANSFORMED PHENOTYPE Edited by A.J. Levine, State University of New York at Stony Brook; G.F. Vande Woude, National Cancer Insti- tute, National Institutes of Health; W.C. Topp, Cold Spring Harbor Laboratory," J.D. Watson, Cold Spring Harbor Laboratory 1984, 385 pp., Bus., index LC 83-26318 CIP B ~ A . ~ E ~ l ~ S & l A a q A L A 4 L ~ d~