A NIH Supported Research Center SAXS … 7...3 1D 2-3D 3D Low angle solution or powder diffraction...
Transcript of A NIH Supported Research Center SAXS … 7...3 1D 2-3D 3D Low angle solution or powder diffraction...
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SAXS Applications 2: BiologicalNon-Crystalline Diffraction
Tom IrvingCSRRI. Dept. BCPS, Illinois Institute of Technology
BioCAT
A NIH Supported Research Center
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SAXS: What is it?
• “Small-angle X-ray Scattering”• Often used to refer to a continuum of
techniques not all of which particularlysmall angle!
• A better term might be “Non-CrystallineDiffraction” (NCD)
• Seems to be some moves to reserve SASfor” Small Angle Solution Scattering”
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1D1D
2-3D2-3D
3D3D
Low angle solution or powder diffraction
large macromolecular assembles, model membranesystems
Fiber diffraction
fiber forming arrays – muscle, collagen, DNA, amyloids,various carbohydrates, often super-macromolecular scale
Single crystal X-ray diffraction
anything that can crystallize, must be (initially) soluble,usually relatively small in comparison to the above, molecularto macromolecular scale
Dimensional Hierarchy of Biophysical (X-ray) techniques
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Model Membrane Systems
• Usually (but not always!) a 1D problem• Biophysics of membrane fluidity• Effects of cholesterol, temperature, pressure• Studies membrane fusion• Understanding phase behavior for novel
drug, DNA delivery systems
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Tarahovsky et al. 2004 Biophys. J. 87:1054
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Why Fiber Diffraction ?
• Atomic level structures from crystallography or NMR =“gold standard” for structural inferences
• But there is a large class of “fibrous proteins” e.g: Actin, myosin, intermediate filaments, microtubules,
bacterial flagella, filamentous viruses, amyloid,collagenous connective tissue
• Will not crystallize but can be induced to form orientedassemblies
• Some systems naturally form ordered systems
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Rosalind Franklin’s Pattern from B-DNA
Franklin & Gosling, 1953 Nature 171:740
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Engineeredamyloid fiber
~10Å
~ 4.7Å
“Cross-beta” Structure
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Potato virus X
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Type I collagen
a b
c
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Insect flight-muscle
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Principles I
Packing of Fibers and Diffraction
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Fibers (essentially rods/cylinders)Usually Hexagonally Packed
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Hexagonal Lattice variables
u
x
y
a→b→
a→ b→
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Ewald Sphere
2 θ
1
h a*
k a*
λ
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End on view of hexagonalreciprocal lattice
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Fiber diagram - Insect Muscle(hexagonal lattice)
Equator
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18I = |FMFL|2
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Principles II
Cylindrical Convolution effects
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X-rays
Fiber Cross-sectionDiffraction
Crystallites randomly orientated around the axisperpendicular to the fiber axis (the ‘R’ – axis: Sum of
Rotation of crystallites assumed = 360 degrees)
R-axis
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Ewald Sphere
2 θ
1
h a*
k a*
λ
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Principles III
Order and disorder in fibrousspecimens
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Ordering in Fibers:
A - Crystallinefiber
B SemicrystallineFiber
C Non-crystallinefiber
A B C
<I(s)> =<|Fm(S)FL(S)|2>
Average over all molecular and lattice orientations
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Dimensional hierarchy of NCD patterns
1D
2-3D
3D
Equator (R-axis)
Meridian (Z-axis)
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B-form DNA
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Insect flight-muscle
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Principles IV
Helical diffraction theory
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Fibrous Proteins Usually ShowHelical Symmetry
P = pitch
p = subunit axialtranslation distance
R = true repeat distance
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Diffraction from a continuousHelix
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Bessel Functions and Layer lines
Transform of a cylinder
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Rosalind Franklin’s Pattern from B-DNA
Franklin & Gosling, 1953 Nature 171:740
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Discontinuous Helices
A set of points that are regularlyspaced along a helical path
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Diffraction From a Discontinuous Helix
α- helix
18 subunits/5 turns
h=1.5 Å
Pitch =5.4 Å
R= 27 Å
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Diffraction from a helix: comparison
The main effect of shifting from a continuous to a discontinuoushelix is to introduce new helix crosses with their originsdisplaced up and down the meridian by a distance 1/p
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Helical Selection RuleWhich Bessel function order will turn up on what layerline for a more complicated helix?For a non-integral helix (repeats after two or moreturns), with u subunits in t turns, allowed Besselfunctions (n) on layer line l are:
l = m.u + n.t
m is an integer indicating translational periodicity indexof helix lattice
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Integral / Non-integral helices
1/p
1/R1/R
m=0
m=-1
m=1
m=0, l=n l = 8m + 3n
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Crystals of Helical Molecules
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Multi-Stranded (coiled coil) Helices
If N strandsOnly everyNth Layer -line allowed
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Geometry of Fiber Patterns
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Fiber Diffraction Often Just Used toFind Gross Molecular Parameters
• In many cases one can make structural inferenceswithout a full-blown structure solution
• Helical parameters in Polyamino-acids and nucleicacids
• Topology of viruses and other large molecularcomplexes
• Test hypotheses concerning influence of inter-filament lattice spacing
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Rosalind Franklin’s Pattern from B-DNA
Layer lines (L) separated by34 Å nm
Meridional (M) reflection at3.4 Å
=> 10 residues/turn
Franklin & Gosling, 1953 Nature 171:740
L
M
M
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Diffraction from Poly L-Alanine-α-helix
1.5 Å residue trans.
Pitch 5.4Å, R= 27Å
18 residues/5 turns
l=18m+5n
10
15
m=1m=0
m=-1
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Often a Modeling Approach isUsed
• Use known information & any highresolution structural information
• Simulate the observed diffraction patternwith a calculated one
• Use simulated annealing or similaralgorithms to minimize differences
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Diffraction Pattern fromOverstretched Rabbit Muscle
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Model of Regulated Thin Filament fromMuscle from Fiber Data
Poole et al. 2006 J Struct Biol. 155:273
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Fiber Crystallography• Most fiber “structures” result of model building
studies• There have been a small number of Fiber
“structure solutions”.• TMV by Stubbs, Caspar, Holmes et al. (1970’s
1980’s)• High resolution structures by Keichi Namba on
bacterial flagella (Yamashita et al., 1998 NatureSB) aligned by high magnetic fields
• Orgel et al. (2001, 2006) MIR structures of TypeI collagen from rat tail tendon
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Tobacco mosaic virus
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Data collection:Data collection:
B7
B7
7
B
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Collagen type I sub-fibrillar structure
Resolution ~ 11 /5.16 Resolution ~ 11 /5.16 ÅÅ
C
a b
c
Orgel et al., 2006 PNAS 103:9001
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Synchrotrons and FiberDiffraction
• Early work all done with conventional sources -why need synchrotron?
• Patterns weak, have high backgrounds, frequentlyhave multiple closely spaced lattices
• Studies benefit from greatly increased beamquality
• Greatly increased flux permits time-resolvedexperiments
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Why X-ray Diffraction ofMuscle?
• Force producing events occur on the timescale of ≤ 1 ms
• Relevant size scale is 5 - 50 nm formolecular machinery
• X-ray diffraction only technique thatallows simultaneous collection ofstructural and physiological informationon this time scale
• Can be used on living systems to do realphysiological experiments
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First Diffraction Pattern UsingSynchrotron Radiation
Equatorial pattern from insect flight muscle
August, 1970, DESYRosenbaum, Holmes,. & Witz (1971).
Nature 230, 434-437.
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X-ray Interference andCrossbridge Motion in
Active Muscle
V. Lombardi, G. Piazzesi, M. Linari.M. Reconditi (Florence), M. Irving
KCLH. Huxley, A. Stewart (Brandeis)
T. Irving (IIT)
BioCAT
A NIH Supported Research Center
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“Swinging Lever ArmHypothesis”
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Muscle Filament Substructure
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Interference from myosin heads onopposite sides of A-band
Fine structure in the145 Åreflection from frogmuscle
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Conclusions• The interference pattern on the meridian can study motion
of crossbridges in living muscle to 100 microsecond timeresolution and 1 Å accuracy
• Crossbridges do, in fact, move axially when musclesallowed to shorten
• Move more at low loads, less at high loads• Unexpected finding that under most physiological
shortening conditions ~5-7 nm stroke size, 6 pN force perbridge
• The 10-12 nm step size expected from crystallographyseen only at very low load
• “The Muscle Problem” essentially solved
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Time-resolved X-rayDiffraction Studies ofDrosophila Indirect
Flight Muscle in vivoMichael DickinsonMark Frye(Caltech)David Maughan(UVM)Gerrie Farman (IIT)Tanya Bekyarova (IIT)David GoreTom Irving(BioCAT/IIT)
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• Small changes in filament length index thick filament stiffness in vivo•Stores elastic strain energy during the wingbeatreducing energy consumption
Time-resolved: 14.5 nm Reflection Spacing
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wing beat phase0.5 1.00.0 1.5 2.0
% in
tens
ity c
hang
e 500
100
0
19.3 nm first row line spot intensity(crossbridge attachment)
upstroke downstroke upstroke downstroke
lengthen shorten lengthen shorten
400
300
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Analysis Software
• Rate limiting step is data analysis• Long tradition of “rolling on your own”• CCP 13 project http://www.ccp13.ac.uk• Comprehensive data extraction suite• Complementary NSF RCN Stubbs
(Vanderbilt) PI will add angulardeconvolution, other features to suite
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References
• Basics:C. Cantor and P. Schimmel “Biophysical Chemistry part II:Techniques for the study of Biological Structure andFunction” Chapter 14. Freeman, 1980
• A terrific introduction to fiber diffraction :John Squire “The Structural Basis of Muscular Contraction”Plenum, 1981
• Definitive Reference on all things non-crystalline:B.K. Vainshtein “Diffraction of X-rays by Chain Molecules”Elsevier, 1966.
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More references:
Good introduction to “Fiber crystallography”:
Chandrasekaran, R. and Stubbs, G. (2001). Fiberdiffraction. in International Tables for Crystallography,Vol. F: Crystallography of Biological Macromolecules(Rossman, M.G. and Arnold, E., eds.), Kluwer AcademicPublishers, The Netherlands, 444-450.