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Transcript of A n° }< - SPring-8bioxtal.spring8.or.jp/ja/SPRUC/SSB_6_3.pdf · Mn 4CaO 5ó ùû ÛÊé ì ÔØ...
BL BL38B1
2016.8.29 ( )
II(PSII)
e-
H+
H2O
(NADPH)
(ATP)
II Photosystem II PSII)
•! •!
O2O2
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1.9Mn4CaO5
Umena, Kawakami, Shen, Kamiya, Nature , 2011
PSII
Mn
Mn
Mn
Mn
Mn
B. Kok, et al, (1970), V. K. Yachandra, et al., (1993) H.Dau, et al., (2007)
Kok cycle model Mn4CaO5-cluster
S1
S0 S2
S4 S3S3
e-
O2
e-
e-
e-
H+
H+
H+
H2O
H2OH+
4Mn ( III3, IV)?
4Mn (III2, IV2)
4Mn (IV4)?
4Mn (III, IV3)
2H20 ! 4H+ + 4 e + O2
Mn
Mn
Mn
III? or IV?
III? or IV?
III? or IV?
III? or IV?
(III, IV3)
5-cluster
MnIII? or IV?III? or IV?MnIII? or IV?MnMnIII? or IV?Mn
Mn Mn
X-ray
Mn metals
"! 4 Mn
X-ray
Fluorescence X-ray
Mn4CaO5-
X-ray absorption spectrum from mixed valence Mn metal complex
"!
•! 55% !
•! Crikshank DPI
0.16 (1.9 , 3WU2)
X
1.887 1.89 1.893 1.896 1.899
norm
aliz
ed F/
Io
wavelength (/ )
Mn (IV)O2
Mn(III) 2O3
Mn (II) O
X
Mn (III)
Mn (II)
Mn (IV)
0
10
20
30
40
50
60
Mn1 Mn2 Mn3 Mn4 Ca
0.900A 1.892A 1.896A
Mn (1.892
Mn Ca
0.900 1.892
PSII X
PSII
•! X X •! •!
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Number of Diffraction image
K-
•! X Mn •! •! Mn X
NSL-193L 193.4nm
Beam size: FWHM 4um Pulse power: 1µ J/pls Pulse width: <1.3ns
1
43
25
:50 µm
PSII
Kitano H. et al., Jpn. J. Appl. Phys. (2005)
•!
!!
!! /
•!
!! (dose)
!!
No Process Cylinder shaperesolution ( ) 50-2.5 (2.54-2.50) mosaic range (°) 0.39-0.43 0.34-0.41 No of reflection 1,075,057 1,100,974 R-merge 0.14 (0.85) 0.09 (0.48) <I>/<! (I)> 15.5 (1.5) 25.2 (2.5) completeness (%) 97.0 (90.3) 98.6 (98.3)
0 0.05 0.1
0.15 0.2
0.25 0.3
0 100 200 300 400 500
R-m
erge
Frame number
0 2 4 6 8
10 12
0 100 200 300 400 500
Scal
e fa
ctor
Frame Number
8 10 10
0.05 0.1 0.1
Advantage of cylinder shaped crystal 1
0
0.05
0.1
0.15
0.2
0.25
0.3
0 30 60 90 120 150
Rm
erge
oscilation range (/degree)
cylinder
none
150 °(30° x 5 crystals)
Advantage of cylinder shaped crystal 2 #!Multi-crystal data collection
#!Accurate X-ray Dose estimation
-0.005
0
0.005
0.01
0.015
0.02
0.025
0.03
6530 6535 6540 6545 6550 6555 6560 6565 6570
0deg_none 45deg_none 90deg_none
-0.005
0
0.005
0.01
0.015
0.02
0.025
0.03
6530 6535 6540 6545 6550 6555 6560 6565 6570
0deg_clynd 45deg_cylnd 90deg_cylnd
045
90
045
90
Advantage of cylinder shaped crystal 3
XAFS spectra at different crystal orientations
* (8 ) * 10cm 2cm
NIR (850nm)
NIR
NIR
•! ! etc
•! !! !!
•! XAFS ! X
