A cell and its population of genes :. DNA forms double strands by a process called hybridization:

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cell and its population of genes:

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Transcript of A cell and its population of genes :. DNA forms double strands by a process called hybridization:

Page 1: A cell and its population of genes :. DNA forms double strands by a process called hybridization:

A cell and its population of genes:

Page 2: A cell and its population of genes :. DNA forms double strands by a process called hybridization:

DNA forms double strands by a process calledhybridization:

Page 3: A cell and its population of genes :. DNA forms double strands by a process called hybridization:

Labeling

Page 4: A cell and its population of genes :. DNA forms double strands by a process called hybridization:

Hybridization

Page 5: A cell and its population of genes :. DNA forms double strands by a process called hybridization:

m109 oligo

placenta complex

Page 6: A cell and its population of genes :. DNA forms double strands by a process called hybridization:

2 color fluoresent dye labeled mRNA

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Affymetrix oligonucleotide arrays

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Page 9: A cell and its population of genes :. DNA forms double strands by a process called hybridization:

Illumina bead arrays

http://www.illumina.com/Images/technology/beadarray_multi_sample_array_formats_lg.gif
Page 10: A cell and its population of genes :. DNA forms double strands by a process called hybridization:
Page 11: A cell and its population of genes :. DNA forms double strands by a process called hybridization:
Page 12: A cell and its population of genes :. DNA forms double strands by a process called hybridization:

• Illumina flyer

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SNP detection

(slide from NCBI,http://www.ncbi.nlm.nih.gov/projects/genome/probe/doc/TechBeadArray.shtml

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RNA-seq

From:RNA-Seq: a revolutionary tool for transcriptomicsZhong Wang, Mark Gerstein & Michael SnyderNature Reviews Genetics 10, 57-63

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Estimation of gene expression from RNA-seq: RPKM values

Number of reads which map per kilobase of exon per million mapped reads for each gene

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Page 17: A cell and its population of genes :. DNA forms double strands by a process called hybridization:

Theoretical Bioinformatics Dr. Benedikt Brors

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Two samples from the same kidney carcinoma

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Cancer – normal comparison

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Cancer-normal comparison: significance?

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MA-plot: Minus vs. Average

(Wikipedia)

http://en.wikipedia.org/wiki/File:Pre-normalization.MAplot.png
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Questions

• Differential genes between two conditions (e.g., healthy/diseased)

• Coexpressed genes: pathways, coregulation

• Genes characteristic for particular conditions (e.g., tumor staging)


somatic hybridization

somatic hybridization

Southern hybridization

Southern hybridization

Lesson Overview Lesson OverviewMeiosis Chromosome Number Chromosomes—those strands of DNA and protein inside the cell nucleus—are the carriers of genes.

Lesson Overview Lesson OverviewMeiosis Chromosome Number Chromosomes—those strands of DNA and protein inside the cell nucleus—are the carriers of genes.

Snake Hybridization

Snake Hybridization

Nucleic Acid Hybridization Nucleic acids Complementary bases Hybridization Complementary strands from any sources Reversible reaction DNA/DNA or DNA/RNA.

Nucleic Acid Hybridization Nucleic acids Complementary bases Hybridization Complementary strands from any sources Reversible reaction DNA/DNA or DNA/RNA.

Isolation and Functional Characterization of genes related to Kernel Development by Differential Hybridization and Yeast Two Hybrid Screening in extra-early.

Isolation and Functional Characterization of genes related to Kernel Development by Differential Hybridization and Yeast Two Hybrid Screening in extra-early.

Jan 13: Genetics--genes & environment Genetics vs ...Jan 13: Genetics--hybridization Hybridization--cont’d Multilocus case (far more common for behavioral traits) •Trait results

Jan 13: Genetics--genes & environment Genetics vs ...Jan 13: Genetics--hybridization Hybridization--cont’d Multilocus case (far more common for behavioral traits) •Trait results

Asecond nitrogenase in vegetative cells of heterocyst ... · Proc. Natl. Acad. Sci. USA92 (1995) Table 1. Hybridization ofDNAfromcyanobacterial strains to nif2 genes ofA. variabilis

Asecond nitrogenase in vegetative cells of heterocyst ... · Proc. Natl. Acad. Sci. USA92 (1995) Table 1. Hybridization ofDNAfromcyanobacterial strains to nif2 genes ofA. variabilis

Distant hybridization

Distant hybridization

Fluorescent cDNA microarray hybridization reveals ... · representing a potentially important class of stress-responsive genes in leukemic cells. Keywords: cDNA microarray; ionizing

Fluorescent cDNA microarray hybridization reveals ... · representing a potentially important class of stress-responsive genes in leukemic cells. Keywords: cDNA microarray; ionizing

subtractive hybridization

subtractive hybridization

Hybridization Theory

Hybridization Theory

Technology for Systems Biology. Nucleic Acid Hybridization In principle complementary strands will associate Chemistry is quite different on surfaces.

Technology for Systems Biology. Nucleic Acid Hybridization In principle complementary strands will associate Chemistry is quite different on surfaces.

Hybridization التهجين

Hybridization التهجين

Widespread hybridization within mound-building wood ants in ...eprints.whiterose.ac.uk/117003/1/Beresford_et_al-2017...exchanged protective colour-pattern genes promiscuously, implying

Widespread hybridization within mound-building wood ants in ...eprints.whiterose.ac.uk/117003/1/Beresford_et_al-2017...exchanged protective colour-pattern genes promiscuously, implying

LECTURE 4. SCREENING cDNA LIBRARIES to ISOLATE NEW GENES: DIFFERENTIAL HYBRIDIZATION ORIGINAL ARTICLE: **Davis, RL, Weintraub, H, and Lassar, A. 1987.

LECTURE 4. SCREENING cDNA LIBRARIES to ISOLATE NEW GENES: DIFFERENTIAL HYBRIDIZATION ORIGINAL ARTICLE: **Davis, RL, Weintraub, H, and Lassar, A. 1987.

Natural Hybridization

Natural Hybridization

Country Home Christmas - Hobby Lobby...Stem Stitch (6 strands 321) Stem Stitch (alternate 6 strands ECRU, with 3 strands 498, and 2 strands 367) Chain Stitch (6 strands ECRU) Stem

Country Home Christmas - Hobby Lobby...Stem Stitch (6 strands 321) Stem Stitch (alternate 6 strands ECRU, with 3 strands 498, and 2 strands 367) Chain Stitch (6 strands ECRU) Stem

Planarian Pax6 genes - Development › content › develop › 129 › 6 › 1423...Planarian Pax6 genes 1425 The following clones were used as probes for in situ hybridization experiments.

Planarian Pax6 genes - Development › content › develop › 129 › 6 › 1423...Planarian Pax6 genes 1425 The following clones were used as probes for in situ hybridization experiments.

NUCLEIC ACIDS HYBRIDIZATION: POTENTIALS AND LIMITATIONS · (which provides column binding by double-stranded nucleic acids). Using puri-fied single-stranded tracer, cDNA second strands

NUCLEIC ACIDS HYBRIDIZATION: POTENTIALS AND LIMITATIONS · (which provides column binding by double-stranded nucleic acids). Using puri-fied single-stranded tracer, cDNA second strands