A Background Ion Reduction Device (BIRD) for nanospray ESI · A Background Ion Reduction Device...
Transcript of A Background Ion Reduction Device (BIRD) for nanospray ESI · A Background Ion Reduction Device...
![Page 1: A Background Ion Reduction Device (BIRD) for nanospray ESI · A Background Ion Reduction Device (ABIRD) ... sources of background ions in the lab. ABIRD ON ... • ABIRD is adaptable](https://reader031.fdocuments.net/reader031/viewer/2022022602/5b52ab827f8b9a56588d9bb3/html5/thumbnails/1.jpg)
A Background Ion Reduction Device (ABIRD) for nanospray ESI
By John Neveu
Harvard University Life Science Division Mass Spectrometry and Proteomics Resource Laboratory
![Page 2: A Background Ion Reduction Device (BIRD) for nanospray ESI · A Background Ion Reduction Device (ABIRD) ... sources of background ions in the lab. ABIRD ON ... • ABIRD is adaptable](https://reader031.fdocuments.net/reader031/viewer/2022022602/5b52ab827f8b9a56588d9bb3/html5/thumbnails/2.jpg)
Nanospray ESI (n-ESI) is a common technique for interfacing capillary chromatography systems
with a mass spectrometer.
n-ESI is typically done without supplied gas, leaving the background ion signal subject to variable
ambient laboratory air conditions.
![Page 3: A Background Ion Reduction Device (BIRD) for nanospray ESI · A Background Ion Reduction Device (ABIRD) ... sources of background ions in the lab. ABIRD ON ... • ABIRD is adaptable](https://reader031.fdocuments.net/reader031/viewer/2022022602/5b52ab827f8b9a56588d9bb3/html5/thumbnails/3.jpg)
ABIRD works by treating the ambient lab air, which is then directed to the n-ESI spray formation zone in an open source.
![Page 4: A Background Ion Reduction Device (BIRD) for nanospray ESI · A Background Ion Reduction Device (ABIRD) ... sources of background ions in the lab. ABIRD ON ... • ABIRD is adaptable](https://reader031.fdocuments.net/reader031/viewer/2022022602/5b52ab827f8b9a56588d9bb3/html5/thumbnails/4.jpg)
ABIRD also works by treating the ambient lab air which then displaces untreated air in a semi closed source.
![Page 5: A Background Ion Reduction Device (BIRD) for nanospray ESI · A Background Ion Reduction Device (ABIRD) ... sources of background ions in the lab. ABIRD ON ... • ABIRD is adaptable](https://reader031.fdocuments.net/reader031/viewer/2022022602/5b52ab827f8b9a56588d9bb3/html5/thumbnails/5.jpg)
Typical Orbitrap full MS no ABIRD
7.59e5
Typical Orbitrap full MS with ABIRD.
5.00e4
ABIRD on an Orbitrap shows greater than a 10x reduction in background ions when operating.
![Page 6: A Background Ion Reduction Device (BIRD) for nanospray ESI · A Background Ion Reduction Device (ABIRD) ... sources of background ions in the lab. ABIRD ON ... • ABIRD is adaptable](https://reader031.fdocuments.net/reader031/viewer/2022022602/5b52ab827f8b9a56588d9bb3/html5/thumbnails/6.jpg)
ABIRD installed on an LTQ shows 10x reduction in background ion signal.
2.36e3
Typical LTQ full MS with ABIRD. Typical LTQ full MS no ABIRD
2.38e4
![Page 7: A Background Ion Reduction Device (BIRD) for nanospray ESI · A Background Ion Reduction Device (ABIRD) ... sources of background ions in the lab. ABIRD ON ... • ABIRD is adaptable](https://reader031.fdocuments.net/reader031/viewer/2022022602/5b52ab827f8b9a56588d9bb3/html5/thumbnails/7.jpg)
ABRID has been demonstrated on the Q-TOF Premier. Note expanded mass range 50-2000Da. ~20x reduction in background ion signal
Typical full MS on Q-TOF Premier no ABIRD Full MS on Q-TOF Premier with ABIRD
6.42e3 3.41e2
![Page 8: A Background Ion Reduction Device (BIRD) for nanospray ESI · A Background Ion Reduction Device (ABIRD) ... sources of background ions in the lab. ABIRD ON ... • ABIRD is adaptable](https://reader031.fdocuments.net/reader031/viewer/2022022602/5b52ab827f8b9a56588d9bb3/html5/thumbnails/8.jpg)
ABIRD significantly improves signal to noise and signal stability across a chromatographic run.
No ABIRD
With ABIRD
![Page 9: A Background Ion Reduction Device (BIRD) for nanospray ESI · A Background Ion Reduction Device (ABIRD) ... sources of background ions in the lab. ABIRD ON ... • ABIRD is adaptable](https://reader031.fdocuments.net/reader031/viewer/2022022602/5b52ab827f8b9a56588d9bb3/html5/thumbnails/9.jpg)
ABIRD isolates the mass spectrometer from transient sources of background ions in the lab.
ABIRD ON
ABIRD OFF: background ion spike and continued rise.
![Page 10: A Background Ion Reduction Device (BIRD) for nanospray ESI · A Background Ion Reduction Device (ABIRD) ... sources of background ions in the lab. ABIRD ON ... • ABIRD is adaptable](https://reader031.fdocuments.net/reader031/viewer/2022022602/5b52ab827f8b9a56588d9bb3/html5/thumbnails/10.jpg)
Current prototype images: Front view of ABIRD device. Approved by mascot, Purdy.
![Page 11: A Background Ion Reduction Device (BIRD) for nanospray ESI · A Background Ion Reduction Device (ABIRD) ... sources of background ions in the lab. ABIRD ON ... • ABIRD is adaptable](https://reader031.fdocuments.net/reader031/viewer/2022022602/5b52ab827f8b9a56588d9bb3/html5/thumbnails/11.jpg)
Rear and side views:
Features include Teflon FEP interface to source, lightweight plastic housing,
power switch, fan speed control. Designed to use inexpensive disposable
cartridge filters which are available in a variety of chemistries.
![Page 12: A Background Ion Reduction Device (BIRD) for nanospray ESI · A Background Ion Reduction Device (ABIRD) ... sources of background ions in the lab. ABIRD ON ... • ABIRD is adaptable](https://reader031.fdocuments.net/reader031/viewer/2022022602/5b52ab827f8b9a56588d9bb3/html5/thumbnails/12.jpg)
ABIRD device in place on LTQ-Orbitrap with a New Objective Picoview source:
![Page 13: A Background Ion Reduction Device (BIRD) for nanospray ESI · A Background Ion Reduction Device (ABIRD) ... sources of background ions in the lab. ABIRD ON ... • ABIRD is adaptable](https://reader031.fdocuments.net/reader031/viewer/2022022602/5b52ab827f8b9a56588d9bb3/html5/thumbnails/13.jpg)
ABIRD device in place on Q-TOF Premier with OEM nanospray source.
Note the simple, clean installation which leaves room for column
compartment and does not interfere with access to nanospray assembly.
(Yes, that is a prototype PicoClear Conductive union at the column and
needle interface. Get one if you can and you’ll never use anything else…)
![Page 14: A Background Ion Reduction Device (BIRD) for nanospray ESI · A Background Ion Reduction Device (ABIRD) ... sources of background ions in the lab. ABIRD ON ... • ABIRD is adaptable](https://reader031.fdocuments.net/reader031/viewer/2022022602/5b52ab827f8b9a56588d9bb3/html5/thumbnails/14.jpg)
Summary:
• ABIRD imparts a controlled low level background ion signal to nanoESI mass spectrometers across a wide mass range.
• ABIRD protects the instrument from ambient spikes in laboratory air quality.
• ABIRD works with either an open or semi-sealed ESI source.
• ABIRD uses inexpensive disposable cartridge filters.
• ABIRD is adaptable to many common ESI sources.