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APPLICATION FORM

YSP-TraveI Fellowships for 13th FAOBMB congress, Bangkoko23-29 November,2012.

Name of Applicant: Ms,A4rlDr. MD RAKIBUL ISLAM

Position: ffi/PostdoctoralFellow(indicatetrainffiglcareerstage)Assistant Professor

Address of Applicant: (Normally this will be your institutional or office address)DEPT. OF BIOCHEMISTRY AND MOLECULAR BIOLOGY,LINIVERSITY OF DHAKA, DHAKA- I OOO, BANGLADESH.

E,mail address: [email protected]

Membership of FAOBMB Constituent Society:FAOBMB in your countrylregion of residence whichProfile under "About FAOBMB: constituenthttp:r'ifaobmb.net

(Inciicate the Constituent Sociefy ofyou are a member). Please see Societies

Memb er" on the webpage at:

Date of Birth: October 12,1980.Applicants must be no more than 35 years of age at the closing date for application:(31 July,2012).

Proof of Age: (Certification by the Head of your Institution or Department is required) Icertify on the basis of official documentation which I have r""n 1e.g. passport, birthcertificate or driver's licence) that the date of birth of the applicant Ms,A4r/Dr. Md. RakibulIslam is 12110/1980, as shown above.

(DD/Month/Year)

Signed:

Position:t{aman Refiid Chowdhury, Ph.D

Professor & ChairrnanPePartrnent Of BiochernistrY

fud Mellecular BiologY

Have you previouslyYtilSffigf,ffit$AoBMB Travel Feilowship? If yes, please indicatewhen.NO. I HVE NOT.

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Curriculum Vitae Provide a brief CV showing education and research training, including any prizes or awards: (no more than one page) Present position: Assistant Professor, Department of Biochemistry and Molecular Biology, University of Dhaka, Bangladesh. Key responsibilities: Teaching and Research. Academic degrees: B. Sc. Honours in Biochemistry and Molecular Biology, University of Dhaka, Bangladesh, 2004. M.S. in Biochemistry and Molecular Biology, University of Dhaka, Bangladesh, 2005. Ph. D. in Medicine, University of Miyazaki, Japan, 2012. Research work: April 2012-till to date: Assistant Professor, Department of Biochemistry and Molecular Biology, University of Dhaka, Bangladesh. April 2008-March 2012: Ph.D. Student, Faculty of Medicine, University of Miyazaki, Japan. October 2007-March 2008: Research fellow, University of Miyazaki Japan. July 2006-March 2012: Lecturer, Department of Biochemistry and Molecular Biology. University of Dhaka, Bangladesh. May 2006- July 2006: Research Officer, Virology Laboratory, Laboratory Science Division (LSD), International Center for Diarrhoeal Disease Research, Bangladesh (ICDDR, B). Project Assigned: ‘Long-term neurological and functional outcome in patients with Nipah virus infections’. March 2005- April 2006: Postgraduate Research Fellow, Molecular Genetics Laboratory, LSD, ICDDR, B. Project Assigned: Prevalence of Vibriophages in Surface Water as an Important Component of Aquatic Microbial Communities Awards and Honors received: Best poster award in the 83rd Annual meeting of the Japan society of Bacteriology, Yokohama, 2010/3/26-28 Awarded the “Monbukagakusho Scholarship” (2007) for Ph.D. at the University of Miyazaki, Japan. Honoured with the 'Kamaluddin Ahmed Gold Medal' for securing the first position at the M.S examination. Society Membership: Bangladesh Society for Biochemistry and Molecular Biology. Graduate Biochemist Association of Bangladesh. Japan Society of Bacteriology. Society of Genome Microbiology Japan.

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List of Publications in International Journals* * Number all publications in international journals or book chapters, with earliest first and most recent at the end. Provide the Impact Factor (IF) of the journal and indicate your contribution the work described and the publication. Use as many pages as required. Follow the style in these examples: 1. K.Y. Soo, J.D. Atkin, M.K. Horne and P. Nagley. Recruitment of mitochondria

into apoptotic signalling correlates with the presence of inclusions formed by amyotrophic lateral sclerosis-associated SOD1 mutations. Journal of Neurochemistry 108, 578-590 (2009) (IF 4.337) PN’s contribution to this work was......

2. A.W. Linnane, A. Baumer, R.J. Maxwell, C. Zhang and P. Nagley. Mitochondrial DNA mutation: the ageing process and degenerative diseases. In New Horizons in Aging Science: Proceedings of the Fourth Asia/Oceania Regional Congress of Gerontology, (H. Orimo, Y. Fukuchi, K. Kuramoto and M. Iriki, eds.), University of Tokyo Press, pp. 85-86 (1992) *****************

1. M.Z.H. Howlader, Y., Kabir, T.A. Khan, M. R. Islam, F. Begum, and F. G. Huffman. Plasma lipid profile, lipid peroxidation and antioxidant status in preeclamptic and uncomplicated pregnancies in Bangladesh. J. Med. Sciences 8: 1576-1582 (2007). MR Islam’s contribution to this work was experiment design and manuscript preparation.

2. M.Z.H. Howlader, M.R. Islam, J. Hossain, T.A. Khan, A.F. Karim, and Y. Kabir. Combined Effects of Type-II Diabetes Mellitus and Cigarette Smoking Increase the Risk of Cardiovascular Complications. International Journal of Diabetes and Metabolism. 15(3): 94-99 (2007) MR Islam’s contribution to this work was experiment design, performing pertial experiments and manuscript preparation.

3. M. Arif, M.R. Islam, T.M.Z. Waise, F. Hassan, S.I. Mondalb, Y. Kabir. DNA damage and plasma antioxidant indices in Bangladeshi type 2 diabetic patients. Diabetes Metab. 36(1): 51-57, (2010). MR Islam’s contribution to this work was experiment design, performing partial experiments and manuscript preparation.

4. K. Murase, T. Ooka, A. Iguchi, Y. Ogura, K. Nakayama, M. Asadulghani,

M.R. Islam, H. Hiyoshi, T. Kodama, L. Beutin, T. Hayashi. Haemolysin E-and enterohaemolysin-derived haemolytic activity of O55/O157 strains and other Escherichia coli lineages. Microbiology. 158(3): 746-758, (2012). MR Islam’s contribution to this work was experiment design, performing partial experiments and manuscript preparation.

5. M.R. Islam, Y. Ogura, M. Asadulghani, T. Ooka, Murase K, Y. Gotoh, T.

Hayashi. A sensitive and simple plaque formation method for the Stx2 phage of Escherichia coli O157:H7, which does not form plaques in the standard plating procedure. Plasmid. 67(3): 227-235, (2012). MR Islam’s contribution to this work was experiment design, performing major part of experiments and manuscript preparation.

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Abstract: Indicate below the abstract that is submitted by you for presentation at the YSP and the FAOBMB Congress in Bangkok (include all authors, affiliation(s) and the text of the abstract)

Systematic functional analysis of the genes in Sp5, the Stx2 phage of O157:H7 Sakai

Md Rakibul Islam,1,2 Yoshitoshi Ogura,2,3 Md Asadulghani,3,4 Kazunori Murase,2 Tadasuke Ooka,2 and Tetsuya Hayashi,2, 3 Department of Biochemistry and Molecular Biology, University of Dhaka, Dhaka, Bangladesh1; Department of Infectious Diseases, Division of Microbiology, Faculty of Medicine, University of Miyazaki, Miyazaki, Japan2; Division of Bioenvironmental Sciences, Frontier Science Research Center, University of Miyazaki, Miyazaki, Japan3; International Center for Diarrhoeal Disease Research, Bangladesh4.

Evolution and the functional significance of bacteriophages in the evolution of

bacteria have intensively been studied over many years, although only a small number

have been investigated in detail. Among 11 lambdoid prophages on the EHEC

O157:H7 Sakai genome, Sp5 and Sp15 encode Stx2 and Stx1, respectively. Although

the early region genes of Sp5, mostly involved in DNA replication and gene

regulations, have high similarity with those of lambdoid phages, but many of late

region genes that are presumably associated with virion formation show no sequence

similarity to known phage genes, and thus their functions are largely unknown.

Therefore, to understand these unknown genes’ roles in Sp5 propagation, we first

constructed an O157:H7 Sakai derivative, in which the stx2 gene on Sp5 was replaced

by the cat-cassette, and lysogenized into E. coli strain K-12. Subsequently, a series of

Sp5 mutants were constructed under the K-12 background, each of which contains an

in-frame deletion of the respective ORF in order to analyze their function(s). We also

developed a modified double agar overlay method using Mitomycin C and CaCl2 (or

MgCl2) that allows us to easily detect and accurately enumerate plaques of Sp5. The

results of systematic analysis of 38 mutants for their DNA-packaging, infection and

plaque formation abilities indicated that several of these genes are required for or

involved in the process of Sp5 propagation. Complementation analyses with wild type

genes confirmed their essentiality in virion production and/or their infectivity,

although complementation was unsuccessful in some of the mutants. We are now

constructing additional mutants to cover all unknown genes of Sp5, including those in

the early region, for better understanding the biology of Stx2-transducing phage of

EHEC O157:H7.

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Personal Statement Indicate briefly here your Research Interests and Career Goals, why you are interested to participate in the YSP Program (including what you will bring to the YSP and what you hope to gain from it): (no more than one page) My research interests and activities over the past six years have mainly centered on the molecular study of the evolution of pathogenic intestinal bacteria and better understand the functional significances of bacteriophages in the evolution of bacteria. In addition to these research areas, I was also actively involved in the finding of naturally occurring molecule that might reduce the severity and the pathogenicity of gut pathogen i.e., Enterohemorrahgic Escherichia coli (EHEC). Since I have a lot of experimental data supporting my goal therefore, with this study I have a plan to develop an antidote that can be use as the treatment of EHEC infection. Beside this I have also the keen interest in the study and elucidation of the molecular mechanism of multidrug resistant bacteria in Bangladesh. Currently, I am working as an assistant Professor in the Department of Biochemistry and Molecular Biology, University of Dhaka, Bangladesh with the key responsibilities of teaching and research. Since YSP-program is aimed at bringing young scientists from FAOBMB regions to come together for discussion and exchange of knowledge and ideas in relation to their research in the field of Biochemistry and Molecular Biology, therefore I strongly believe that my participation will help me to enrich my knowledge and skill in the field of molecular biology and I could share this with the student at the university level. Beside this I will be able to gain some ideas in current research trend and the residing problems in the Asian and Oceanian countries by exchanging the views with the young participants. My experiences in molecular biology will enable me to collaborate with clinicians, local and international scientists to conduct transdisciplinary studies in basic science and might help in our national developments. My participation will also help me immensely and boost up to run educational arts activities and events in my own county. Moreover, along with my teaching I aspire to become an independent researcher and have my own lab therefore, this YSP- program participation will help me a lot to achieve my goals. I will show my research data and the expertise that I have in the field of molecular biology will be shared with the young scientists from all over the Asian and Oceanian region. I strongly believe that this scientific sharing will help many of the young participants that in turn will help for their national developments.

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Attachments:

• Letters of recommendation from two referees.

Submission methods: You can apply in either of two ways:

• by sending an email with scanned attachments to the Chair of the FAOBMB Fellowship Committee, Prof. Piamsook Pongsawasdi: [email protected] or [email protected] This is the preferred method. If using this method, please assemble the Application Form and the Attachments into a single PDF file.

• by sending a hard copy of the application form and supporting documents to: Professor Piamsook Pongsawasdi, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand.

Closing Date: Applications must be received by 31 July, 2012 (Bangkok time, GMT + 7 Hours). Applicants will be notified by email of the decision of the Committee by no later than 15 September, 2012.

Department of Biochemistryand Molecular BiologyUniversity of DhakaDhaka-1000Bangladesh.

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Recommendation letter for Dr. Md. Rakibul Islam

It is my pleasure to write this recommendation letter for Dr. Md. Rakibul Islam who is known tome first as a student and then as a faculty colleague in the University of Dhaka. He has completedhis Bachelor of Science and Master of Science from the Department of Biochemistry andMolecular Biology, University of Dhaka securing first class first position in both examinations.Recently he has got his Ph.D. in Medicine from the University of Miyazaki, JAPAN. Dr. Islamhas an excellent academic record all along with great research interest and I always found him tobe a very intelligent and competent. After completion of his M.S. degree he joined in theDepartment of Biochemistry and Molecular Biology as a Lecturer and recently promoted to an

Assistant professor with the responsibilities of teaching and research.

Dr. Islam is a highly motivated young man with intellectual capability and he is always ready toaccept challenges with zeal, enthusiasm and dedication to achieve his objectives. Hiscommunication skill in English is very good. He is not just a promising young scientist with highpotential but also a well-mannered person with a maturity of personality and integrity ofcharacter.

I strongly recommend for providing Dr. Islam an opportunity to participate in the Young ScientistProgram (YSP) will be held during November 2012 at the Sirindhorn Science Home in Bangkok.His knowledge and training at this Young Scientist Program would definitely benefit him and as

well as his institution.

I wish him every success in future.

Mamun Rashid Chowdhury, Ph.D.Professor and Chairm dn,Department of Biochemistry and Molecular BiologyFaculty of Biological SciencesIJniversity of DhakaDhaka - 1000

Bangladesh.

986 ^ 920-73. Ext 7636, Fax ' 880-2-861 5583 lnternet . http:l lbrnbudhaka.net

Department of Biochemistryand Molecular BiologyUniversity of DhakaDhaka-1000Bangladesh.

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Recommendation letter for Dr. Md. Rakibul Islam

I am delighted to write this recommendation letter for Dr. Md. Rakibul Islam who has beenknown to me as students and colleague as well in the department of Biochemistry andMolecular Biology, University of Dhaka. He has completed his four Bachelor of Scienceand Master of Science from this department and recently he has got his Ph.D. in Medicinefrom the University of Miyazaki, JAPAN. At present time he is working as an AssistantProfessor in the same department with key responsibility of teaching and research.

Dr. Islam is very sincere, honest and is highly motivated with has deep sense of devotion tohis teaching *d research work. Throughout his stay in this department I found his keeninterest in the field of Molecular Biology. It is my strong belief that if he gets properopportunities and facilities he will be able to prove his worth and can achieve his long-termgoal.

I strongly feel that if he gets the opportunity to participate in the Young Scientist Program(YSP) that will be held during November 2012 in Bangkok, he will be able to contribute in thedevelopment of his home country with the skills and qualification that he will obtained fromthe participation.

I wish him all the success in life.

Prof. Ishtiaq Mahmud Ph.D.ProfessorDepartment of Biochemistry and Molecular BiologyFaculty of Biological SciencesIJniversity of DhakaDhaka - 1000

Bangladesh.

Te e3none : 9661920-T 3, Ext. 7636, Fax : 880-2-8615583 I nternet . http://brnbudhaka. net

7 Islam Rakibul

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