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6th Annual Stem Cells & Regenerative Medicine Meeting Conference Presentation
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Transcript of 6th Annual Stem Cells & Regenerative Medicine Meeting Conference Presentation
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ESC-Derived Cells For Restoration of Tissues & Organs
Robert Lanza, MDVP Research & Scientific DevelopmentAdvanced Cell Technologyand Adjunct ProfessorWake Forest University School of Medicine
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Alzheimer’s
Dwarfism
Parkinson’s
Strokes
Epilepsy
Hemophilia
Kidney failure
Chronic pain
CancerInfertility
Burns
AIDS
Muscular dystrophy
ALS
Affective disorders
Macular degeneration
Hypoparathyroidism
Heart disease
Liver failure
Enzymatic defects
Diabetes
Osteoarthritis
Multiple sclerosis
Huntington’s
Hypocholesterolemia
Rheumatoid
arthritis
Atherosclerosis
Ulcers
Spinal cord
injuries
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Anatomy & Function of RPE
• Immune barrier • Absorption of stray light• Vit A metabolism & transport• Phagocytosis of shed photoreceptor segments
FUNCTIONS OF RPE
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Diseases Associated with RPE dysfunction
• Age-related macular degeneration• Retinitis pigmentosa• Stargardt's disease• Best's vitelliform macular dystrophy• Leber's congenital amaurosis
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Transplantation of RPE in Humans
Associated problemsSources of RPE cells
* autologous tissue
* cell lines
* donor tissue (adult, fetal) safety ethical Batch-to-batchvariation
may have impaired function limited supply
Potential tumorigenicity
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Advantages of ECS-derived Tissues for RegenerativeMedicine
• Unlimited supply• Can be derived under GMP conditions pathogen-
free• Can be produced with minimal batch to batch
variation• Can be thoroughly characterized to ensure optimal
performance
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[All hES cell lines studied reproducibly generated RPE lines that could be passaged, characterized, and expanded]
•WiCell hES cell lines (23 RPE lines generated) WA01 WA09 WA07 •Harvard hES cell lines (22 RPE lines generated) HUES1 HUES6 HUES2 HUES7 HUES3 HUES8 HUES5 HUES10
•ACT hES cell lines (25 RPE lines generated) MA01 MA03 MAJ1 MA04 MA09 MA14 MA40
RPE can be generated from hES cells
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Stages of RPE isolation from spontaneously differentiating hES cells
35 mm plate one of the clustersone of the clusters cell suspension at plating
4 days
x100x200
x200
7 days
x200
Passage 1 -- 25 daysPassage 1 -- 25 days
x200
x0.75
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x400
x200
hES-RPE express RPE markers (bestrophin &CRALBP)
-- 32
-- 46
-- 78
CRALBP
Mw
a b c
bestrophin
bestrophin
CRALBP
Immunostaining Western blot
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PEDF RPE65
RT-PCR
1 2 1 2
1 – fetal RPE2 – hES-RPE
hES-RPE express RPE65 and PEDF
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DB
X15,200
x7000
Phagocytosis of latex beads (electron microscopy)
Latex beadspigment
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hES-RPE vs. its in vivo counterpart
RPE hES-RPEcobblestone, pigmented
transdifferentiation-differentiation
phagocytosis
molecular markers
RPE65
CRALBP
bestrophin
PEDF
MERTK
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Gene expression profiling of hES-RPE vs. their in vivocounterpart
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RPE transplantation into subretinal space of RCS rats (in collaboration with Raymond Lund, University of Utah)
RCS rats naturally become blind in several weeksdue to RPE degeneration and photoreceptor death
Study designcell line RPE (H9) Control: culture medium
Tests: head tracking (behavior)electroretinogram (ERG) histology
In vitro assessment:molecular markers of RPEmorphology and behavior
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1 2 3 1 2 3 1 2 3 1 2 3 1 2 3 1 2 3
RPE65
bestro
phin
CRALBP
PEDFPax
6
GAPDH
H9 RPE used for transplantation in RCS rats
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conehES-RPE
ERG at P60
Am
plitu
de (u
V)
40
a-hES-RPE a-sham b-hES-RPE b-sham cone b-sham
180
020
6080
120140160
100
hES-RPE transplantation into subretinal space of RCS rats
Optomotor at P100
hES-RPE Sham Untreated
0.5
0.3
0.4
0.2
0
0.1
Rel
ativ
e ac
uity
(c/d
)
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Summary
• hES-RPE is similar to its in vivo counterpart by multiple parameters (morphology, behavior, phagocytosis, molecular markers)
• hES-RPE can be reproducibly generated from hES cells
• hES-RPE attenuates photoreceptor loss in animal model of retinal degeneration
hES-RPE advantages
• Minimize batch-to-batch variation• Can be derived under GMP conditions• Can be produced from feeder-free hES cells• Can be easily generated in large quantities (for pathogen & safety assessment, and pre-clinical & clinical studies)
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Hemangioblasts derived from hESC
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Hemangioblasts Generated from Human ES Cells
40X 10X
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• Established an efficient method to generate hemangioblasts from multiple hESC lines
• Cells can be easily & reproducibly scaled up
• Hemangioblasts derived from hESC form functional hematopoietic & endothelial cells in vitro
• Rapidly repair damaged vasculature in vivo (ischemic limbs, retina, and myocardium)
Summary
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Generation of ES cells using parthenogenesis
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WBC colony from cloned stem cells
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•Cardiovascular disease costs the US $329 billion annually
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Waiting List
Organs Transplanted
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End-stage renal disease will cost US $1 trillion during the comingdecade