600 PATENT ABSTRACTS

A method and apparatus are provided for selec- ting and analyzing a subpopulation of cells or cell objects for a certain parameter such as DNA using image analysis means. The cells are first stained with an alkaline phosphatase technique including a monoclonal antibody specific to a protein in at least one of the cell's cytoplasm or on a cell membrane, thereby marking any cells including the protein as to type. A second staining of the DNA in the nucleus is ac- complished by a Feulgen technique that destroys the cell cytoplasm. After the staining and mar- king, the cells may then be gated using the image analysis means on the visual parameter such as colored DNA or colored antigen into a sub- population that is to be measured. The selected cells may then be examined by digital image pro- cessing and measured for a parameter such as a true actual measurement of DNA in picograms. A quantitation of the measured parameter may be generated and provided.

5281518

D E T E C T I O N O F A U N I Q U E C H L A M Y D I A S T R A I N

A S S O C I A T E D W I T H A C U T E R E S P I R A T O R Y D I S E A S E

Lee Campbell, Chou-chou Kuo, Thoma Grayston assigned to Washington Research Foundation

Methods for detecting a unique strain of Chlamydia associated with acute respiratory dis- ease are disclosed. These methods utilize mono- clonal antibody directed against an antigenic determinant of the TWAR organism, or DNA probes capable of specifically hybridizing to at least a portion of the DNA sequence of the TWAR organism. A method for determining the presence of antibodies to the TWAR organism, utilizing elementary bodies of the TWAR or- ganism as antigen is also disclosed.

5281521

M O D I F I E D A V I D I N - B I O T I N T E C H N I Q U E

John Trojanowski, Virginia M-Y Lee assigned to The Trustees of The University of Pennsyl- vania

A method for preparing an antigen specific probe is provided by incubating a primary anti-

gen specific monoclonal antibody with a bio- tinylated secondary antibody to produce a complex of the primary and secondary anti- bodies. The staining pattern produced by these probes reflects the specificity of the monoclonal antibody in the complex and the labeling of ir- relevant, endogenous immunoglobulins is reduced substantially. This novel, indirect immunohistochemical method can be used to study normal and diseased tissues using a variety of monoclonal antibodies.

5281525

C E P H A L O S P O R I N A C E T Y L H Y D R O L A S E G E N E F R O M B A C I L L U S S U B T I L I S

Kenji Mitsushima, Akio Takimoto, Shigeo Yagi, Takayasu Sonoyama, Osaka, Japan as- signed to Shionogi & Co Ltd

A cephalosporin acetylhydrolase gene, a recom- binant DNA molecule prepared by introducing said gene into a vector used in an E. coil host- vector system, E. coil cells transformed with said recombinant DNA molecule, a protein having the amino acid sequence encoded by said gene, an enzyme which is a multimer of said protein, and a process for preparing said protein or en- zyme are provided, said cephalosporin ac- etylhydrolase being an enzyme useful for converting cephalosporins such as cephalosporin C and 7-ACA into deacetylated ones such as deacetylcephalosporin C and deacetyl 7-ACA which are useful as an inter- mediate for preparing a variety of derivatized cephalosporin antibiotics.

5281582

S E R U M G R O W T H F A C T O R

Philip Dehazya assigned to Alliance Pharma- ceuticals Corp

A novel glycosylated form of albumin designated EGA and isolated from biological fluids is further characterized by its cell growth promoting activity. A process of isolation of human or bovine glycosylated albumin is described. The EGA fractions have been iden- tified by serological histochemical and biological assays as well as lectin reactivity. The growth- promoting effect of EGA is directed to various transformed cell lines and primary cells of roam-

PATENT ABSTRACTS 601

malian origin. Hepatoma cells have been found to produce EGA, in vitro. Novel compositions of EGA containing media are provided for cell, tissue or organ culture.

5281661

C O M P L E X C O N T A I N I N G C O A G U L A T I O N F A C T O R IX

Yendra Linnau, Mafia Sazgary, Vienna, Austria assigned to Immuno Aktiengesellschaft

Factor IX is selectively adsorbed by means of hydrophobic chromatography from an aqueous mixture containing at least one plasma zymogen or a vitamin-K dependent protein in addition to factor IX. By this method, the efficient enrich- ment of factor IX for the production of pharma- ceutical preparations has become possible.

mer particles comprising polymeric core particles coated evenly with a layer of polymer containing magnetically responsive metal oxide. A wide variety of polymeric particles with sizes ranging from 1 to 100 microns can be used a core particles and transformed into magnetically responsive polymer particles. The surface of these magnetically responsive polymer particles can be coated further with another layer of func- tionalized polymer. These magnetically respon- sive fluorescent polymer particles can be used for passive or covalent coupling of biological material such as antigens, antibodies, enzymes or DNA/RNA hybridization and used as solid phase for various types of immunoassays, DNA/RNA hybridization probes assays, af- finity purification, cell separation and other medical, diagnostic, and industrial applications.

5283172

5283066

M E T H O D O F S T I M U L A T I N G AN I M M U N E R E S P O N S E BY U S I N G A

H A P T E N

Jiuan Liu, Chun-Chie Wang, Yuang-Lin Hwang, Tong H Chang, ,anneiV assigned to Development Center for Biotechnology

Disclosed is a method of stimulating an immune response by using a hapten. Haptens are adhered onto a solid phase support to form a complex. This complex can stimulate an immune response of an animal and the animal's lymphocytes. The solid phase supports usable in the method pre- ferably are membranes, latex particles or micro- particle beads of hydrophilic cellulose mixed ester, nitrocellulose, cellulose acetate, nylon, polyvinylidone, vinylbenzyl chloromethyl styrene, polyacrolein, a ahydrophobic, poly- tetrafluoroethylene, polystyrene and silica gel.

5283079

P R O C E S S T O M A K E M A G N E T I C A L L Y R E S P O N S I V E

F L U O R E S C E N T P O L Y M E R P A R T I C L E S

Chao-Huei J Wang, Dinesh O Shah assigned to Baxter Diagnostics Inc

This invention provides a novel process of pro- ducing magnetically responsive fluorescent poly-

T Y P E - C R O T A V I R U S C U L T U R E S A N D U S E S T H E R E F O R

Mark Welter, David M Chambers, C Joseph Welter assigned to Ambico Inc

Type-C rotavirus are propagated in swine testicular cells with reduced concentrations of proteolytic enzyme for subsequent production of antigen and antiserum for use in diagnostic kits and for killed vaccines to prevent Type C rotavirus infections. Propagation of Type C rotavirus in the ST cells can also lead to virus modification for subsequent use as a modified live virus vaccine to prevent Type C rotavirus in- fections.

5283190

S P E C I F I C M O N O C L O N A L A N T I B O D I E S A G A I N S T A

D E F I N E D E P I T O P E O F P R O G E S T E R O N E R E C E P T O R

A N D M E T H O D S F O R T H E I R U S E

Adbulmaged M Traish, Herbert Wotiz

The present invention provides unique prepared immunogens, site-specific monoclonal anti- bodies against an epitope of progesterone recep- tor protein, and an immunoassay to determine the functional integrity of the progesterone receptors in a cellular sample. Collectively or in- dividually the component parts of the invention provide the ability not only to identify accurately