PATENT ABSTRACTS

This invention relates to mutants or variants of Bacillus thuringiensis producing high amounts of active delta-endotoxins. The delta-endotoxins produced by the mutant or variant B. thuringiensis will have an activity directed towards the same pest insects as its parent B. thuringiensis delta-endotoxins, such as against lepidopterans (mutants from B. thuringiensis subsp, kurstaki or subsp, aizawai), dipterans (mutants from B. thuringiensis subsp, is- raelensis) or coleopterans (mutants from B. thuringlensis subsp, tenebrionis).

563

This invention also provides a method of stimulating nerve cell growth using purified F- spondin. This invention further provides a method of regenerating nerve call using purified F-spondin. Finally, this invention provides a pharmaceutical composition for stimulating nerve cell growth comprising an effective amount of purified F-spondin and a pharma- ceutically acceptable carrier.

5280112

5279965

R E C O M B I N A N T I N F E C T I O U S L A R Y N G O T R A C H E I T I S V I R U S

D N A S E Q U E N C E E N C O D I N G B O V I N E A N D H U M A N

A D R E N O C O R T I C O T R O P I C H O R M O N E R E C E P T O R S

Calvin L Keeler

The present invention is concerned with the pre- paration of an Infectious Laryngotracheitis Virus (1LTV) mutant which does not produce a functional thymidine kinase enzyme as a result of a mutation in the TK gene, and its prepara- tion. The invention also relates to an ILTV mutant containing a heterologous gene encoding an antigen of an avian pathogen incorporated into the TK gene. Such an ILTV mutant can be applied as a vector vaccine to induce an immune response after infection of an appropriate host animal.

5279966

C L O N I N G , E X P R E S S I O N A N D U S E S O F A N O V E L S E C R E T E D

P R O T E I N , F ' S P O N D I N

Thomas Jessell, Avihu Klar assigned to The Trustees of Columbia University in the City of New York

This invention provides an isolated vertebrate nucleic acid molecule encoding F-spondin. This invention also provides a probe comprising a nucleic acid molecule of at least 15 nucleotides capable of specifically hybridizing with a sequence included within the sequence of a nucleic acid molecule encoding a F-spondin. This invention provides a host vector system for the production of a F-spondin'. This invention provides purified F-spondin and the uses of com- positions containing purified F-spondin. This in- vention further provides a method of attaching nerve cells to a matrix using purified F-spondin.

Roger D Cone, Kathleen G Mountjoy assigned to State of Oregon Oregon Health Sciences University

The present invention relates to a mammalian adrenocorticotropic hormone receptor. The in- vention is directed toward the isolation, charac- terization and pharmacological use of mammalian adrenocorticotropic hormone receptor, the gene corresponding to this recep- tor, a recombinant eukaryotic expression con- struct capable of expressing a mammalian adrenocorticotropic hormone receptor in cul- tures of transformed eukaryotic cells and such cultures of transformed eukaryotic cells that syn- thesize mammalian adrenocorticotropic hor- mone receptor. The invention also provides methods for screening ACTHR agonists and an- tagonists in vitro using preparations of receptor from such cultures of eukaryotic cells trans- formed with a recombinant eukaryotic expression construct comprising the ACTHR receptor gene. The invention specifically pro- vides human and bovine ACTHR genes.

5281516

T E M P E R A T U R E C O N T R O L A P P A R A T U S A N D M E T H O D

Marilyn Stapleton, Warren Jewett assigned to Gene Tec Corporation

An apparatus and method for performing auto- mated sample preparation, DNA amplification and detection, which apparatus has heat- sinking, fiat carriers for holding specimens and reagents, devices for heating and cooling and

564 PATENT ABSTRACTS

maintaining the specimen to or at any given tem- perature for any given time periods, and a computer to generate signals that control said temperatures and times.

presence of antibodies to the TWAR organism, utilizing elementary bodies of the TWAR or- ganism as antigen is also disclosed,

5281520

5281517

M E T H O D S F O R I M M U N O P L O I D Y A N A L Y S I S

James W Bacus, Robert Marder assigned to Cell Analysis Systems Inc

A method and apparatus are provided for selec- ting and analyzing a subpopulation of cells or cell objects for a certain parameter such as DNA using image analysis means. The ceils are first stained with an alkaline phosphatase technique including a monoclonal antibody specific to a protein in at least one of the cell's cytoplasm or on a cell membrane, thereby marking any cells including the protein as to type. A second staining of the DNA in the nucleus is ac- complished by a Feulgen technique that destroys the cell cytoplasm. After the staining and mar- king, the cells may then be gated using the image analysis means on the visual parameter such as colored DNA or colored antigen into a sub- population that is to be measured. The selected cells may then be examined by digital image pro- cessing and measured for a parameter such as a true actual measurement of DNA in picograms. A quantitation of the measured parameter may be generated and provided.

M E T H O D F O R P R O D U C I N G A C Y L O X Y A C Y L H Y D R O L A S E

Patrick O'Hara, Frederick Hagen, Francis J Grant, Robert Munford assigned to ZymoGenetics Inc; Board of Regents University of Texas Syst

Methods are disclosed for producing ac- yloxyacyl hydrolase. The protein is produced from eukaryotic host cells transformed or trans- fected with DNA construct(s) containing information necessary to direct the expression of acyloxyacyl hydrolase. The DNA constructs generally include the following operably linked elements: a transcriptional promoter; DNA sequence encoding acyloxyacyl hydrolase, the small subunit of acyloxyacyl hydrolase or the large subunit of acyloxyacyl hydrolase; and a transcriptional terminator. In addition, isolated DNA sequences encoding acyloxyacyl hydrolase and isolated DNA sequences encoding the small or large subunit of acyloxyacyl hydrolase are dis- closed.

5281525

C E P H A L O S P O R I N A C E T Y L H Y D R O L A S E G E N E F R O M B A C I L L U S S U B T I L I S

5281518

D E T E C T I O N O F A U N I Q U E C H L A M Y D I A S T R A I N

A S S O C I A T E D W I T H A C U T E R E S P I R A T O R Y D I S E A S E

Lee Campbell, Chou-chou Kuo, Thoma Grayston assigned to Washington Research Foundation

Methods for detecting a unique strain of Chlamydia associated with acute respiratory dis- ease are disclosed. These methods utilize mono- clonal antibody directed against an antigenic determinant of the TWAR organism, or DNA probes capable of specifically hybridizing to at least a portion of the DNA sequence of the TWAR organism. A method for determining the

Kenji Mitsushima, Akio Takimoto, Shigeo Yagi, Takayasu Sonoyama, Osaka, Japan as- signed to Shionogi & Co Ltd

A cephalosporin acetylhydrolase gene, a recom- binant DNA molecule prepared by introducing said gene into a vector used in an E. coli host- vector system, E. coli cells transformed with said recombinant DNA molecule, a protein having the amino acid sequence encoded by said gene, an enzyme which is a multimer of said protein, and a process for preparing said protein or en- zyme are provided, said cephalosporin ac- etylhydrolase being an enzyme useful for converting cephalosporins such as cephalosporin C and 7-ACA into deacetylated ones such as deacetylcephalosporin C and deacetyl 7-ACA which are useful as an inter- mediate for preparing a variety of derivatized cephalosporin antibiotics.