525 MaintMan LibBlue 600328 Rev 3PB - CEM...

32www.cempeptides.com • [email protected] • (800) 726-3331

(US + Canada) • (704) 821-7015 Copyright CEM Corporation 2015 • 600388 • Revision 3

Copyright 2015 by CEM CorporationAll Rights Reserved

This manual contains proprietary information which shall notbe reproduced or transferred to other documents or disclosed to

others without prior written permission of CEM Corporation.CEM® and Discover® are registered trademarks of CEM Corporation.

600328 ∙ December 2015 ∙ Rev. 3 ∙ Copyright CEM Corporation 2015

Liberty Blue™Automated Microwave Peptide Synthesizer

Maintenance Manual



Contents3 Safety Information3 Safety Notations3 Fume Ventilation3 Waste Disposal

4 System Requirements4 Bench and/or Fume Hood Space4 Environmental Conditions4 Inert Gas Source4 Electrical Requirements4 Fuse Replacement

5 How to Obtain Support5 Applications Support5 Technical Support5 Requested Information6 Contact CEM

7 Recommended Routine Maintenance Schedule7 Daily Maintenance7 Biweekly Maintenance7 Monthly Maintenance8 Semiannual Maintenance

8 Standby Procedure

8 Maintenance Procedures9 Backfl ush10 Timed Addition Calibration12 Verifi cation of Filtered Drain13 Verifi cation of Waste Sensor Operation14 Reaction Vessel Cleaning Procedure

16 Maintenance Records

29 Liberty Blue System Schematic

30 Liberty Blue Flow Diagram

31 Warranty and Service Information



Warranty InformationLimited WarrantyWhat Is Covered:CEM Corporation warrants that the instrument will be free of any defect in parts or workmanship and will, at its option, replace or repair any defective part (excluding consumables) or instrument.

For How Long:This warranty remains in effect for 365 days from date of delivery to the original purchaser.

What Is Not Covered:This warranty does not cover parts or workmanship damaged due to:• Neglect, abuse or misuse, or theft• Damage caused by or to test samples,• Damage incurred during instrument relocation,• Damage caused by or to any attached equipment,• Use of incorrect line voltages or fuses,• Fire, fl ood, “acts of God” or other contingencies beyond the control of CEM Corporation,• Improper or unauthorized repair, or• Any other damage caused by purchaser or its agents.

Responsibilities of Purchaser:• To ensure warranty coverage, the purchaser must:• Use the instrument according to directions,• Connect the instrument properly to a power supply of proper voltage,• Replace blown fuses,• Replace consumables and• Clean the instrument as required.

How to Get Service:Purchaser should contact the Service Department of CEM Corporation or the nearest CEM subsidiary or distributor for return authorization and for proper crating and shipping instructions to return instrument, freight prepaid, for service. On-site repairs by an authorized service technician are available through the CEM Service Department. Travel costs will be charged to the purchaser for onsite repairs.

Within the U.S. Outside the U.S.CEM Corporation CEM Corporation3100 Smith Farm Rd. 3100 Smith Farm Rd.Matthews, NC 28104-5044 Matthews, NC 28104-5044(800) 726-5551 (704) 821-7015Fax: (704) 821-4368 Fax: (704) 821-4368

Warranty Disclaimer:CEM Corporation hereby excludes and disclaims any warranty of merchantability or fi tness for any particular purpose. No warranty, express or implied, extends beyond the face hereof. CEM Corporation shall not be liable for loss of use of instrument or other incidental or consequential costs, expenses or damages incurred by the purchaser or any other user. This warranty is not transferable.

Purchaser’s Rights under State Law:This warranty gives the purchaser specifi c legal rights, and the purchaser may also have other rights that vary from state to state.



Liberty Blue Flow Diagram



Safety InformationSafety NotationsThis manual uses three safety alert words at points in the documentation where the user should be aware of potential hazards. The safety alerts are shown in color-coded boxes. The three words–NOTE, CAUTION, and WARNING–indicate differing levels of observation or action as described below:

Personal Laboratory SafetySolvents and chemical reagents used on the Liberty Blue can be hazardous and should only be handled under a fume hood. Personal protective gear, including but not limited to safety glasses, chemical resistant gloves, and a laboratory coat are highly recommended when operating the Liberty Blue. For detailed information on the safety requirements for the chemicals used on the Liberty Blue, refer to the appropriate SDS documents.

Fume VentilationThe Liberty Blue operates as a semi-closed system, with minimal venting of any hazardous solvent fumes through the vent lines coming from the side of the unit. The vent lines must be vented into a proper chemical fume hood or exhaust line no longer than six feet (6’)/two meters (2 m) from the instrument.In addition, adequate ventilation should be provided for preparation of reagents and solvents for use on the system. All solvent bottles and the Waste Reservoir should be placed into proper secondary containers to minimize the risk of exposure.

Waste DisposalWaste produced by the Liberty Blue can be hazardous. For detailed information on the safety requirements for the chemicals used on the Liberty Blue, refer to the appropriate SDS documents.

NOTEA NOTE is intended to provide emphasis of procedures that may be misinterpreted or overlooked, or to clarify confusing situations.

CAUTIONA CAUTION is intended to provide essential information and to emphasize procedures which, if not strictly followed, may result in improper instrument operation.

WARNINGA WARNING is intended to emphasize dangerous or hazardous conditions which may result in personal injury to the user and damage or destruction of the instrument.

WARNINGHandle all waste under a fume hood, and wear suitable protective clothing such as safety glasses, chemical resistant gloves, and a laboratory coat. Dispose of all waste in accordance with all applicable local, state, and federal health and safety recommendations



System Requirements

Bench and/or Fume Hood SpaceThe Liberty Blue should be positioned on the bench such that access to the electrical outlets for the system is not restricted. The Liberty Blue requires the following space for system components:

Liberty Blue Instrument (Discover Module and Liberty Blue Module):21.8” (w) x 21.4” (d) x 30” (h) [55.3 cm (w) x 54.32 cm (d) x 76.2 cm (h)](Depth includes 3” (7.62 cm) clearance behind instrument for unimpeded airfl ow at rear fan ducts)

CEM Supplied Controller:Laptop: 12.5” (w) x 9.9” (d) x 15.5” (h) [32 cm (w) x 25 cm (d) x39 cm (h)] (Height includes 14.1” (35.8 cm) display)

Environmental ConditionsThe Liberty Blue is designed for indoor used only.Temperature Range: 50 °F – 85 °F (10 °C – 29 °C)Relative Humidity Range: 10% – 85%

Inert Gas SourceThe Liberty Blue requires an inert gas source (either high purity grade nitrogen or argon) capable of supplying 25 psi (20 L/min fl ow) within ten feet (10’)/three meters (3 m) of the right side of the instrument.

Electrical RequirementsThe Liberty Blue requires electrical power of 120 VAC (60 Hz, 1.7 A) (or 240 VAC [50 Hz, 1.7 A] where applicable). Specifi c power requirements (120 VAC vs. 240 VAC) can be found on the nameplate affi xed to the rear of the Liberty module and on the side of the Discover module.Three (3) grounded electrical connections providing a total of 10 A are required for all components:• Liberty Blue power cord• Router power cord• Controller (laptop computer) power cord

Fuse ReplacementThe Liberty Blue has four operator replaceable fuses located in the power inlet module (at the rear of the instrument, where the power cord connects to the Liberty Blue module). The fuses are rated F 250 V 2 A. The top pair of fuses (PN BR198826) are North American style, 5 mm x 20 mm size, and are rated F 250 V 2 A. The bottom pair of fuses (PN 188340) are North American style, 5 mm x 20 mm size, and are rated F 250 V 10 A.

NOTEOptionally, the router can be powered directly from the Liberty Blue Module without the need for an external electrical connection (using Router-Serial Power Cable, PN 243290).



L1L2

L3L4

L5L6

L7L8

L9L1

0

L20

L19

L18

L17

L16

L15

L14

L13

L12

L11

L27

L26

L25

L24

L23

L22

L21

AC

TA

CT

-B

PS

1

PS

2P

S3

MS

IM

S2

DV

VA

AP

AA

PR

GP

MA

INR

V1

VP

S

0

5

10

1520

25

30

13

5

7

CT

R

1

2

3

4

5

RO

T1

RO

T2

WA

ST

ED

EP

RO

MA

INS

OLV

EN

T

REAGENTWASHVENT

FR

IT

LIQ-NMS-LPRG

RV-W

DP-NC

DP-NDP-V

VAA1

VAA2

PAA

PS2

PRG

DP-NREG

R1-3R2-3

RV-V2RV-V3

RV

-MA

NIF

OLD

LS2

LS1

VAA2

PS2

VA

A3

R1-

2

RV

-V1

RV

-V2

FIL

TE

R

R15

NIT

RO

GE

N

LIB

ER

TY

-BLU

E

CO

MP

LEX

FLU

IDIC

DIA

GR

AM

RV-V1RV-WR1-1

R2-CTR

LS3

R2-

10U

V

MS

-L

MS-N-W

DP-NC

DP-VUV

VAA3RV-V3

FLT

N2

SY

S

RV

AA

VNTSYS

UVRV

DV

EX

TE

RN

AL

M

AN

IFO

LD

LS-5

LS-L

1

LS-S

2(S

pill-

Sen

sors

)

RE

V-B

8

910

6

4

2

MS-N-WMS-N-N2

MS-N-N2

MS

-P

R1-

3

R1-4

R1-4W-V

W-V

R1-2

R1-

1

R1-5

R2-CTR

R2-

3

R2-5

R2-5

R2-

1

R2-1

DEP-LIQDEP-N2

MAIN-LIQMAIN-N2

R2-

10

R2-6

Liberty Blue System Schematic



Libe

rty

Blue

Mai

nten

ance

Cha

rt f

or t

he M

onth

of

12

34

56

78

910

1112

1314

1516

1718

1920

2122

2324

2526

2728

2930

31

Insp

ect

the

inst

rum

ent

for

leak

age

in a

ll sp

ill t

rays

and

in t

he D

isco

ver

Cav

ity.

Ensu

re t

he fi

ber-

opti

c pr

obe

is f

ully

in

sert

ed in

to t

he t

herm

owel

l.

Back

fl ush

all

posi

tion

s ex

cept

Mai

n W

ash/

DM

F.

Perf

orm

all

tim

ed a

ddit

ion

calib

rati

ons.

Veri

fy p

erfo

rman

ce o

f th

e fi l

tere

d dr

ain.

Insp

ect

the

reac

tion

ves

sel f

or

part

icul

ate

accu

mul

atio

n.

Revi

ew r

un h

isto

ry r

epor

ts t

o en

sure

th

at t

he L

iber

ty B

lue

is h

eati

ng

prop

erly

.

Perf

orm

VPS

Was

h op

erat

ion.

Perf

orm

Ext

ende

d W

ash

Rota

ry

Valv

es o

pera

tion

.

Repl

ace

the

amin

o ac

id d

ip t

ubes

fi l

ters

.

Repl

ace

the

DM

F fi l

ter.

Repl

ace

the

reac

tion

ves

sel b

ody.

Veri

fy t

he p

rope

r op

erat

ion

of t

he

was

te s

enso

r.



How to Obtain Support

Applications SupportFor the latest Liberty Blue applications information, go to http://www.cem.com/liberty-blue.html. The CEM Liberty Blue pages contain downloadable applications notes, a listing of recent microwave peptide synthesis publications, and more. A registered account is required for download.CEM is proud to provide applications support for any peptide synthesis related questions from a team of trained chemists with a complete peptide synthesis lab. For applications support, call (800) 726-3331 (inside the US) or (704) 821-7015 and ask for “Peptide Applications”, or email CEM Liberty Blue applications support at [email protected].

Technical SupportFor the latest technical support information, go to http://www.cemservice.us. The CEM Service website provides access to the CEM Knowledge Base, which contains helpful troubleshooting information. From the website requests for phone or email support can also be submitted.CEM is proud to provide technical support for the Liberty Blue from a team of specially trained Service Technicians. For technical support in the US and Canada, call (800) 726-5551 or (704) 821-7015 and ask for “Liberty Blue Service”. For technical support outside the US and Canada, contact your local CEM Subsidiary or Distributor.

Requested InformationWhen contacting CEM for support, please provide the following information about the instrument:• Liberty Blue Serial Number• Liberty Blue Firmware Version• Discover Serial Number• Discover Firmware Version• Liberty Blue Software Version Number and Build Number• The run history report for the synthesis that was running

when the error occurred• Troubleshooting Bundle (see below)

Firmware versions (both Liberty Blue and Discover) and Liberty Blue software version can be found within Liberty Blue Software from the About Menu. Serial numbers can be found on the silver tags on the rear of the Liberty Blue and on the side of the Discover.In the event of a crash, the software will automatically create a Troubleshooting Bundle to be emailed to CEM support for diagnosis. A Troubleshooting Bundle can be manually created from the Help Menu.



Contact CEMCEM Corporation HeadquartersToll-Free Phone(US/Canada): (800) 726-3331Phone: (704) 821-7015Service Hotline: (800) 726-5551Fax: (704) 821-7894Mailing Address:PO Box 200Matthews, NC 28106-0200Physical Address:3100 Smith Farm RdMatthews, NC 28104Email (Applications Support):[email protected] (Technical Support):[email protected] International SubsidiariesFrance: CEM mWaves S.A.S.Phone: (33-1) 69 35 57 80Fax: (33-1) 60 19 64 91Address:Immeuble Ariane Domaine Technologique de Saclay4, rue René Razel91892 ORSAY CedexFranceWeb Address: http://www.cemfrance.frEmail: [email protected]

Germany: CEM GmbHPhone: 011-49-2842-9644-0Fax: 011-49-2842-9644-11Address:Carl-Friedrich-GauB-Str. 947475 Kamp-LintfortGermanyWeb Address: http://www.cem.deEmail: [email protected]

Italy: CEM SRLPhone: 390-35-896224Fax: 390-35-891661Address:Via Dell Artigianato, 6/8

24055 Cologno Al Serio (BG)ItalyWeb Address:http://www.cemmicroonde.itEmail: [email protected]

Japan: CEM Japan K.K.Phone: +81-3-5793-8542Fax: +81-3-5793-8543Address:2-18-10 TakanawaMinato-KuTokyo108-0074JapanWeb Address:http://www.cemjapan.co.jpEmail: [email protected]

UK: CEM Microwave Technology Ltd.Phone: +44-1-280-822873Fax: +44-1-280-822342Address:2 Middle SladeBuckingham Industrial Park MK18 1WABuckinghamGreat BritainWeb Address:http://www.cemmicrowave.co.ukEmail: [email protected]

Ireland: CEM Technology (Ireland) Ltd.Phone: +353 (0) 1 885-1752Address:Sky Business Centre9a Plato Business ParkDamastown Dublin 15IrelandWeb Address:http://www.cemmicrowave.co.ukEmail: [email protected]

CEM DistributorsFor a complete list of distributors of CEM products, including contact information, go to the CEM website (http://www.cem.com), select Contact, and then select your region to see a list of distributors by country.



Libe

rty

Blue

Mai

nten

ance

Cha

rt f

or t

he M

onth

of

12

34

56

78

910

1112

1314

1516

1718

1920

2122

2324

2526

2728

2930

31

Insp

ect

the

inst

rum

ent

for

leak

age

in a

ll sp

ill t

rays

and

in t

he D

isco

ver

Cav

ity.

Ensu

re t

he fi

ber-

opti

c pr

obe

is f

ully

in

sert

ed in

to t

he t

herm

owel

l.

Back

fl ush

all

posi

tion

s ex

cept

Mai

n W

ash/

DM

F.

Perf

orm

all

tim

ed a

ddit

ion

calib

rati

ons.

Veri

fy p

erfo

rman

ce o

f th

e fi l

tere

d dr

ain.

Insp

ect

the

reac

tion

ves

sel f

or

part

icul

ate

accu

mul

atio

n.

Revi

ew r

un h

isto

ry r

epor

ts t

o en

sure

th

at t

he L

iber

ty B

lue

is h

eati

ng

prop

erly

.

Perf

orm

VPS

Was

h op

erat

ion.

Perf

orm

Ext

ende

d W

ash

Rota

ry

Valv

es o

pera

tion

.

Repl

ace

the

amin

o ac

id d

ip t

ubes

fi l

ters

.

Repl

ace

the

DM

F fi l

ter.

Repl

ace

the

reac

tion

ves

sel b

ody.

Veri

fy t

he p

rope

r op

erat

ion

of t

he

was

te s

enso

r.



Libe

rty

Blue

Mai

nten

ance

Cha

rt f

or t

he M

onth

of

12

34

56

78

910

1112

1314

1516

1718

1920

2122

2324

2526

2728

2930

31

Insp

ect

the

inst

rum

ent

for

leak

age

in a

ll sp

ill t

rays

and

in t

he D

isco

ver

Cav

ity.

Ensu

re t

he fi

ber-

opti

c pr

obe

is f

ully

in

sert

ed in

to t

he t

herm

owel

l.

Back

fl ush

all

posi

tion

s ex

cept

Mai

n W

ash/

DM

F.

Perf

orm

all

tim

ed a

ddit

ion

calib

rati

ons.

Veri

fy p

erfo

rman

ce o

f th

e fi l

tere

d dr

ain.

Insp

ect

the

reac

tion

ves

sel f

or

part

icul

ate

accu

mul

atio

n.

Revi

ew r

un h

isto

ry r

epor

ts t

o en

sure

th

at t

he L

iber

ty B

lue

is h

eati

ng

prop

erly

.

Perf

orm

VPS

Was

h op

erat

ion.

Perf

orm

Ext

ende

d W

ash

Rota

ry

Valv

es o

pera

tion

.

Repl

ace

the

amin

o ac

id d

ip t

ubes

fi l

ters

.

Repl

ace

the

DM

F fi l

ter.

Repl

ace

the

reac

tion

ves

sel b

ody.

Veri

fy t

he p

rope

r op

erat

ion

of t

he

was

te s

enso

r.



Recommended Routine Maintenance ScheduleA routine maintenance protocol is vital for the long-term operation of the Liberty Blue. Maintenance logs for twelve months are provided in the back of this manual for verifi cation that the instrument is properly maintained to ensure optimal operation and performance.

Daily MaintenanceInspect the instrument for any visible signs of leakage in all spill trays, any fl at areas inside the Liberty Blue, and inside the cavity of the Discover. Ensure the fi ber optic probe is fully inserted into the thermowell on the reaction vessel.

Biweekly MaintenanceBackfl ush all positions except Main Wash (see Maintenance Procedures).

Monthly Maintenance• Perform a timed addition calibration for all timed additions (see Timed

Addition Calibrations).• Verify the performance of the fi ltered drain (see Verifi cation of Filtered

Drain).• Inspect the reaction vessel for particulate accumulation, particularly on

the top and sides of the vessel.• Review recent run history reports to ensure that the Liberty Blue is

heating properly. (See Liberty Blue Operations Manual, PN 600291).• Perform an Extended Wash Rotary Valves operation to wash the Rotary 1

and Rotary 2 valve heads with increased volumes of Main Wash Solvent. These volumes can be changed in Settings (Settings → Liberty Blue → Wash).

• Perform a VPS Wash operation to wash the VPS valve and purge the amino acid vent line with Main Wash Solvent (Settings → Liberty Blue → Wash).

WARNINGEnsure that the open end of the amino acid (AA) vent line is placed in a suitable waste container before proceeding with the VPS Wash operation. After completing the operation, the AA vent line should be returned to a proper chemical fume hood or exhaust line.

WARNINGHandle all chemicals under a fume hood, and wear suitable protective clothing such as safety glasses, chemical resistant gloves, and a laboratory coat. Dispose of all waste in accordance with all applicable local, state, and federal health and safety recommendations. For detailed information on the safety requirements for the chemicals used on the Liberty Blue, refer to the appropriate SDS documents.



Semiannual Maintenance• Replace the reagent dip tube fi lters.• Replace the Main Solvent fi lter.• Replace the reaction vessel body.• Verify the proper operation of the waste sensor (see Verifi cation of

Waste Sensor Operation).

Standby Procedure

If the Liberty Blue will be idle for a period of two weeks or more:1. Remove all reagents (amino acids, activator, activator base) from the

system.2. Use the Change Bottle Deprotection operation to replace the

deprotection bottle with an empty bottle and properly clear the lines.3. Backfl ush all positions, including deprotection.

4. Use the Change Bottle Main Solvent operation to remove the wash solvent and connect an empty bottle and properly clear the lines.

5. Use the Depressurize System operation to depressurize the system.

Prior to using the Liberty Blue after it has been in standby:1. Connect the main wash solvent using the Change Bottle Main Solvent

operation to properly prime the lines and pressurize the bottles.2. Perform a backfl ush on all positions.3. Perform a Timed Addition Calibration for all timed additions.

Maintenance Procedures

Backfl ushFrom the Cleaning tab of the Maintenance Screen (found under the Options menu), a backfl ush can be performed on any reagent position except deprotection. (Deprotection can be backfl ushed from the Operations Tab.)

NOTEDuring the priming step, the system will get an Empty Deprotection Bottle Error. This is normal. Click Cancel to clear the error and end the Change Bottle operation.

NOTEDuring the priming step, the system will get an Empty Solvent Bottle Error. This is normal. Click Cancel to clear the error and end the Change Bottle operation.



Libe

rty

Blue

Mai

nten

ance

Cha

rt f

or t

he M

onth

of

12

34

56

78

910

1112

1314

1516

1718

1920

2122

2324

2526

2728

2930

31

Insp

ect

the

inst

rum

ent

for

leak

age

in a

ll sp

ill t

rays

and

in t

he D

isco

ver

Cav

ity.

Ensu

re t

he fi

ber-

opti

c pr

obe

is f

ully

in

sert

ed in

to t

he t

herm

owel

l.

Back

fl ush

all

posi

tion

s ex

cept

Mai

n W

ash/

DM

F.

Perf

orm

all

tim

ed a

ddit

ion

calib

rati

ons.

Veri

fy p

erfo

rman

ce o

f th

e fi l

tere

d dr

ain.

Insp

ect

the

reac

tion

ves

sel f

or

part

icul

ate

accu

mul

atio

n.

Revi

ew r

un h

isto

ry r

epor

ts t

o en

sure

th

at t

he L

iber

ty B

lue

is h

eati

ng

prop

erly

.

Perf

orm

VPS

Was

h op

erat

ion.

Perf

orm

Ext

ende

d W

ash

Rota

ry

Valv

es o

pera

tion

.

Repl

ace

the

amin

o ac

id d

ip t

ubes

fi l

ters

.

Repl

ace

the

DM

F fi l

ter.

Repl

ace

the

reac

tion

ves

sel b

ody.

Veri

fy t

he p

rope

r op

erat

ion

of t

he

was

te s

enso

r.



Libe

rty

Blue

Mai

nten

ance

Cha

rt f

or t

he M

onth

of

12

34

56

78

910

1112

1314

1516

1718

1920

2122

2324

2526

2728

2930

31

Insp

ect

the

inst

rum

ent

for

leak

age

in a

ll sp

ill t

rays

and

in t

he D

isco

ver

Cav

ity.

Ensu

re t

he fi

ber-

opti

c pr

obe

is f

ully

in

sert

ed in

to t

he t

herm

owel

l.

Back

fl ush

all

posi

tion

s ex

cept

Mai

n W

ash/

DM

F.

Perf

orm

all

tim

ed a

ddit

ion

calib

rati

ons.

Veri

fy p

erfo

rman

ce o

f th

e fi l

tere

d dr

ain.

Insp

ect

the

reac

tion

ves

sel f

or

part

icul

ate

accu

mul

atio

n.

Revi

ew r

un h

isto

ry r

epor

ts t

o en

sure

th

at t

he L

iber

ty B

lue

is h

eati

ng

prop

erly

.

Perf

orm

VPS

Was

h op

erat

ion.

Perf

orm

Ext

ende

d W

ash

Rota

ry

Valv

es o

pera

tion

.

Repl

ace

the

amin

o ac

id d

ip t

ubes

fi l

ters

.

Repl

ace

the

DM

F fi l

ter.

Repl

ace

the

reac

tion

ves

sel b

ody.

Veri

fy t

he p

rope

r op

erat

ion

of t

he

was

te s

enso

r.



To perform a backfl ush:1. Replace the centrifuge tubes or bottles for positions to be backfl ushed

with empty tubes or bottles.2. Ensure that all reagent positions have a bottle connected.3. From the Cleaning Tab, check the box next to each position to be

backfl ushed. To backfl ush all reagents, check the box at the top of the table to select all positions.

4. Click the Perform Backfl ush button. A warning box will pop up. Verify that empty bottles have been connected and tightened for all positions to be backfl ushed, and that there is suffi cient solvent for the operation. Click OK to continue.

5. A progress window will pop up showing the progress of the backfl ush procedure for all amino acids.

As each position is backfl ushed, the Last Backfl ush date will be updated to the current date and time.

6. Once all reagent positions have been backfl ushed, the manifold will be cleared, and the system will perform a backpurge on selected positions.

7. Once all positions have been backpurged, remove the bottles from those positions and dispose of the liquid.



To Perform a Backfl ush Deprotection:1. From the Operations Tab, under Backfl ush/Backpurge, select the

Backfl ush Deprotection operation, then click Run Operation.

2. The software will prompt to verify the reaction vessel is empty. Ensure the reaction vessel is empty, then click OK to continue.

3. Solvent will be added to the reaction vessel, then backpurged into the deprotection bottle. To avoid fi lling the lines with the solvent, the software will prompt to replace the bottle. Replace the bottle, then click OK to complete the backfl ush operation.

Timed Addition CalibrationThere are four timed additions that must be calibrated for optimal functionality of the Liberty Blue: Wash, Wash Thru Manifold, Deprotection, and Wash Thru RV Bottom. To calibrate a timed addition:

1. Place the reaction vessel into the volume calibration stand (PN 551055).1.1. Remove the reaction vessel assembly from the cavity of the

Discover. 1.2. Insert the reaction vessel into the calibration stand so that the

drain line from the bottom of the vessel fi ts into the slot on the right of the stand.

2. From the Calibration screen, under the Timed Addition folder, select the addition to be calibrated. The table on the right will update with the calibration history for the selected addition.



Libe

rty

Blue

Mai

nten

ance

Cha

rt f

or t

he M

onth

of

12

34

56

78

910

1112

1314

1516

1718

1920

2122

2324

2526

2728

2930

31

Insp

ect

the

inst

rum

ent

for

leak

age

in a

ll sp

ill t

rays

and

in t

he D

isco

ver

Cav

ity.

Ensu

re t

he fi

ber-

opti

c pr

obe

is f

ully

in

sert

ed in

to t

he t

herm

owel

l.

Back

fl ush

all

posi

tion

s ex

cept

Mai

n W

ash/

DM

F.

Perf

orm

all

tim

ed a

ddit

ion

calib

rati

ons.

Veri

fy p

erfo

rman

ce o

f th

e fi l

tere

d dr

ain.

Insp

ect

the

reac

tion

ves

sel f

or

part

icul

ate

accu

mul

atio

n.

Revi

ew r

un h

isto

ry r

epor

ts t

o en

sure

th

at t

he L

iber

ty B

lue

is h

eati

ng

prop

erly

.

Perf

orm

VPS

Was

h op

erat

ion.

Perf

orm

Ext

ende

d W

ash

Rota

ry

Valv

es o

pera

tion

.

Repl

ace

the

amin

o ac

id d

ip t

ubes

fi l

ters

.

Repl

ace

the

DM

F fi l

ter.

Repl

ace

the

reac

tion

ves

sel b

ody.

Veri

fy t

he p

rope

r op

erat

ion

of t

he

was

te s

enso

r.



Libe

rty

Blue

Mai

nten

ance

Cha

rt f

or t

he M

onth

of

12

34

56

78

910

1112

1314

1516

1718

1920

2122

2324

2526

2728

2930

31

Insp

ect

the

inst

rum

ent

for

leak

age

in a

ll sp

ill t

rays

and

in t

he D

isco

ver

Cav

ity.

Ensu

re t

he fi

ber-

opti

c pr

obe

is f

ully

in

sert

ed in

to t

he t

herm

owel

l.

Back

fl ush

all

posi

tion

s ex

cept

Mai

n W

ash/

DM

F.

Perf

orm

all

tim

ed a

ddit

ion

calib

rati

ons.

Veri

fy p

erfo

rman

ce o

f th

e fi l

tere

d dr

ain.

Insp

ect

the

reac

tion

ves

sel f

or

part

icul

ate

accu

mul

atio

n.

Revi

ew r

un h

isto

ry r

epor

ts t

o en

sure

th

at t

he L

iber

ty B

lue

is h

eati

ng

prop

erly

.

Perf

orm

VPS

Was

h op

erat

ion.

Perf

orm

Ext

ende

d W

ash

Rota

ry

Valv

es o

pera

tion

.

Repl

ace

the

amin

o ac

id d

ip t

ubes

fi l

ters

.

Repl

ace

the

DM

F fi l

ter.

Repl

ace

the

reac

tion

ves

sel b

ody.

Veri

fy t

he p

rope

r op

erat

ion

of t

he

was

te s

enso

r.



3. Click the Calibrate button to begin the calibration operation.4. Enter the volume to be used for calibration, then click OK. Typically, 5

mL of reagent are used for calibration.

5. The selected reagent will be added to the reaction vessel. Once the addition is fi nished, measure the volume delivered to the reaction vessel using the graduations on the calibration stand.

6. Enter the volume into the Delivered Volume box.

7. Click OK drain the reaction vessel. The software will then calculate the new fl ow rate. Click OK to save the new calibration values.

8. Once the new rate has been saved, click OK to complete the operation.

NOTEThe Calibration History table displays a record of all previous calibrations values. The current calibration value is shown in bold. To revert to an older calibration value, double-click on the entry in the table. The selected calibration value should become bold.



Verifi cation of Filtered Drain1. Place the reaction vessel into the volume calibration stand (PN

551055). 1.1. Remove the reaction vessel assembly from the cavity of the

Discover. 1.2. Insert the reaction vessel into the calibration stand so that

the drain line from the bottom of the vessel fi ts into the slot on the right of the stand.

2. From the Options menu, open the Maintenance screen. 3. Under Addition, select the Add Main Wash (MS2) operation.

4. On the Parameters tab, double click the value for Volume, enter 10 in the pop-up, then click OK.

5. Click the Run Operation button to execute the operation. This will add 10 mL of solvent to the vessel.

6. Click the Drain button at the bottom of the Maintenance screen.7. The liquid should drain within 10 seconds. If the liquid requires more

than 10 seconds to fully drain, replace the reaction vessel body.



Libe

rty

Blue

Mai

nten

ance

Cha

rt f

or t

he M

onth

of

12

34

56

78

910

1112

1314

1516

1718

1920

2122

2324

2526

2728

2930

31

Insp

ect

the

inst

rum

ent

for

leak

age

in a

ll sp

ill t

rays

and

in t

he D

isco

ver

Cav

ity.

Ensu

re t

he fi

ber-

opti

c pr

obe

is f

ully

in

sert

ed in

to t

he t

herm

owel

l.

Back

fl ush

all

posi

tion

s ex

cept

Mai

n W

ash/

DM

F.

Perf

orm

all

tim

ed a

ddit

ion

calib

rati

ons.

Veri

fy p

erfo

rman

ce o

f th

e fi l

tere

d dr

ain.

Insp

ect

the

reac

tion

ves

sel f

or

part

icul

ate

accu

mul

atio

n.

Revi

ew r

un h

isto

ry r

epor

ts t

o en

sure

th

at t

he L

iber

ty B

lue

is h

eati

ng

prop

erly

.

Perf

orm

VPS

Was

h op

erat

ion.

Perf

orm

Ext

ende

d W

ash

Rota

ry

Valv

es o

pera

tion

.

Repl

ace

the

amin

o ac

id d

ip t

ubes

fi l

ters

.

Repl

ace

the

DM

F fi l

ter.

Repl

ace

the

reac

tion

ves

sel b

ody.

Veri

fy t

he p

rope

r op

erat

ion

of t

he

was

te s

enso

r.



Libe

rty

Blue

Mai

nten

ance

Cha

rt f

or t

he M

onth

of

12

34

56

78

910

1112

1314

1516

1718

1920

2122

2324

2526

2728

2930

31

Insp

ect

the

inst

rum

ent

for

leak

age

in a

ll sp

ill t

rays

and

in t

he D

isco

ver

Cav

ity.

Ensu

re t

he fi

ber-

opti

c pr

obe

is f

ully

in

sert

ed in

to t

he t

herm

owel

l.

Back

fl ush

all

posi

tion

s ex

cept

Mai

n W

ash/

DM

F.

Perf

orm

all

tim

ed a

ddit

ion

calib

rati

ons.

Veri

fy p

erfo

rman

ce o

f th

e fi l

tere

d dr

ain.

Insp

ect

the

reac

tion

ves

sel f

or

part

icul

ate

accu

mul

atio

n.

Revi

ew r

un h

isto

ry r

epor

ts t

o en

sure

th

at t

he L

iber

ty B

lue

is h

eati

ng

prop

erly

.

Perf

orm

VPS

Was

h op

erat

ion.

Perf

orm

Ext

ende

d W

ash

Rota

ry

Valv

es o

pera

tion

.

Repl

ace

the

amin

o ac

id d

ip t

ubes

fi l

ters

.

Repl

ace

the

DM

F fi l

ter.

Repl

ace

the

reac

tion

ves

sel b

ody.

Veri

fy t

he p

rope

r op

erat

ion

of t

he

was

te s

enso

r.



Verifi cation of Waste Sensor OperationThe Waste Sensor is a fl oat sensor located on the top of the waste bottle. It is connected to the Liberty Blue through a 9-pin connector cable plugged into the left side of the Liberty Blue module. Periodically, operation of the Waste Sensor should be verifi ed. To verify the operation of the Waste Sensor:

1. Ensure the Liberty Blue is idle.2. Remove the cap from the waste container.3. Lift the fl oat sensor.

4. The software should prompt a Waste Full error. In addition, the Waste indicator will turn red.

5. If the Waste Full error does not prompt, ensure the 9-pin connector is securely plugged into the Liberty Module. If the plug is secure and the error does not prompt, replace the waste sensor assembly.

6. Release the fl oat sensor, connect the cap to the waste container, then click OK to clear the Waste Full error.



WARNINGHandle all waste under a fume hood, and wear suitable protective clothing. Dispose of all waste in accordance with all applicable local, state, and federal health and safety recommendations.

Reaction Vessel Cleaning ProcedureThe Liberty Blue™ reaction vessel frit may get clogged with resin beads or with chemical residue over time. A clogged reaction vessel frit may result in increased drain times or the inability to drain completely. CEM recommends cleaning the reaction vessel at the fi rst sign of increased drain times, or after 600 amino acid cycles.

All required parts can be located in the Liberty Blue accessory kit (PN 909430).

1. Remove the reaction vessel from the Discover cavity and rinse with DMF to remove any loose resin. Pour off the solvent into a waste container.

2. Assemble the syringe assembly:

a. Detach the shorter piece of tubing from the fi lter tube assembly (PN 516025) at the white lure fi tting.

b. Attach the 1/8” syringe to nut adapter (PN 164830) to the open nut on the tubing line. Firmly twist the syringe (PN 164215) on to the adapter.

3. Place the reaction vessel into the RV calibration assembly (PN 551055) and attach the syringe assembly to the short tube at the bottom of the vessel.



Libe

rty

Blue

Mai

nten

ance

Cha

rt f

or t

he M

onth

of

12

34

56

78

910

1112

1314

1516

1718

1920

2122

2324

2526

2728

2930

31

Insp

ect

the

inst

rum

ent

for

leak

age

in a

ll sp

ill t

rays

and

in t

he D

isco

ver

Cav

ity.

Ensu

re t

he fi

ber-

opti

c pr

obe

is f

ully

in

sert

ed in

to t

he t

herm

owel

l.

Back

fl ush

all

posi

tion

s ex

cept

Mai

n W

ash/

DM

F.

Perf

orm

all

tim

ed a

ddit

ion

calib

rati

ons.

Veri

fy p

erfo

rman

ce o

f th

e fi l

tere

d dr

ain.

Insp

ect

the

reac

tion

ves

sel f

or

part

icul

ate

accu

mul

atio

n.

Revi

ew r

un h

isto

ry r

epor

ts t

o en

sure

th

at t

he L

iber

ty B

lue

is h

eati

ng

prop

erly

.

Perf

orm

VPS

Was

h op

erat

ion.

Perf

orm

Ext

ende

d W

ash

Rota

ry

Valv

es o

pera

tion

.

Repl

ace

the

amin

o ac

id d

ip t

ubes

fi l

ters

.

Repl

ace

the

DM

F fi l

ter.

Repl

ace

the

reac

tion

ves

sel b

ody.

Veri

fy t

he p

rope

r op

erat

ion

of t

he

was

te s

enso

r.



Libe

rty

Blue

Mai

nten

ance

Cha

rt f

or t

he M

onth

of

12

34

56

78

910

1112

1314

1516

1718

1920

2122

2324

2526

2728

2930

31

Insp

ect

the

inst

rum

ent

for

leak

age

in a

ll sp

ill t

rays

and

in t

he D

isco

ver

Cav

ity.

Ensu

re t

he fi

ber-

opti

c pr

obe

is f

ully

in

sert

ed in

to t

he t

herm

owel

l.

Back

fl ush

all

posi

tion

s ex

cept

Mai

n W

ash/

DM

F.

Perf

orm

all

tim

ed a

ddit

ion

calib

rati

ons.

Veri

fy p

erfo

rman

ce o

f th

e fi l

tere

d dr

ain.

Insp

ect

the

reac

tion

ves

sel f

or

part

icul

ate

accu

mul

atio

n.

Revi

ew r

un h

isto

ry r

epor

ts t

o en

sure

th

at t

he L

iber

ty B

lue

is h

eati

ng

prop

erly

.

Perf

orm

VPS

Was

h op

erat

ion.

Perf

orm

Ext

ende

d W

ash

Rota

ry

Valv

es o

pera

tion

.

Repl

ace

the

amin

o ac

id d

ip t

ubes

fi l

ters

.

Repl

ace

the

DM

F fi l

ter.

Repl

ace

the

reac

tion

ves

sel b

ody.

Veri

fy t

he p

rope

r op

erat

ion

of t

he

was

te s

enso

r.



1. Remove the syringe from the tubing line and fi ll with approximately 10-15mL DMF, then reattach the syringe.

2. Push the liquid from the syringe into the tubing line so that it fl ows into the reaction vessel and bubbles just over the frit. The bubbling will remove loose resin or residual material out of the frit. Discard the waste solvent into a waste container.

3. Repeat the bubbling procedure with approximately 10-15mL MeOH. This procedure may need to be repeated several times with MeOH to dislodge any particles that have been caught in the reaction vessel frit. Discard the waste solvent into a waste container each time.



Maintenance RecordsThe following pages are provided for a monthly record of routine maintenance procedures. As each procedure is completed as indicated in the record, either a check, a date, or the user’s initials should be entered in the appropriate box to record the maintenance procedure as completed. (The blue areas of each calendar page require no action.)

In the event that a Service Technician is required on-site, these records should be available for inspection to ensure that required maintenance has been performed on the instrument.

X

X

X

X

X

X

X

X

X

X

X

X

X

X

X

X

X

X

X

X

X

X

X

X

X

X

X

X

X

X

X

X

X

X

X

X

X

X

X

X

X

X

X

X

X

X

X

X

X

X

X

X

X

X

X

X

X

X

X

X

X

X

X

X

X

X

X

X

X

X

April 2015



Libe

rty

Blue

Mai

nten

ance

Cha

rt f

or t

he M

onth

of

12

34

56

78

910

1112

1314

1516

1718

1920

2122

2324

2526

2728

2930

31

Insp

ect

the

inst

rum

ent

for

leak

age

in a

ll sp

ill t

rays

and

in t

he D

isco

ver

Cav

ity.

Ensu

re t

he fi

ber-

opti

c pr

obe

is f

ully

in

sert

ed in

to t

he t

herm

owel

l.

Back

fl ush

all

posi

tion

s ex

cept

Mai

n W

ash/

DM

F.

Perf

orm

all

tim

ed a

ddit

ion

calib

rati

ons.

Veri

fy p

erfo

rman

ce o

f th

e fi l

tere

d dr

ain.

Insp

ect

the

reac

tion

ves

sel f

or

part

icul

ate

accu

mul

atio

n.

Revi

ew r

un h

isto

ry r

epor

ts t

o en

sure

th

at t

he L

iber

ty B

lue

is h

eati

ng

prop

erly

.

Perf

orm

VPS

Was

h op

erat

ion.

Perf

orm

Ext

ende

d W

ash

Rota

ry

Valv

es o

pera

tion

.

Repl

ace

the

amin

o ac

id d

ip t

ubes

fi l

ters

.

Repl

ace

the

DM

F fi l

ter.

Repl

ace

the

reac

tion

ves

sel b

ody.

Veri

fy t

he p

rope

r op

erat

ion

of t

he

was

te s

enso

r.

525 MaintMan LibBlue 600328 Rev 3PB - CEM...

Documents

Transcript of 525 MaintMan LibBlue 600328 Rev 3PB - CEM...