132

5155037

I N S E C T S I G N A L S E Q U E N C E S U S E F U L T O I M P R O V E T H E

E F F I C I E N C Y O F P R O C E S S I N G A N D S E C R E T I O N O F F O R E I G N

G E N E S I N I N S E C T S Y S T E M S

Max D Summers assigned to The Texas A&M University System

The engineering of foreign vertebrate gene con- structs by recombinant DNA techniques for the more efficient processing and secretion of fore- ign genes in insect systems is done by replacing the foreign vertebrate protein signal peptide sequences with protein signal sequences from in- sect cell secreted proteins. A baculovirus expres- sion vector system is constructed wherein the natural signal DNA peptide sequence associated with the desired foreign gene, such as CD4 (T cell surface protein T4) is replaced by the signal DNA sequence from the insect signal peptides coding for the cuticle gene or adipokinetic hor- mone.

5155041

C U L T U R E O F B A C I L L U S S U B T I L I S

Song H Bok, Sung U Kim, Kwang H Son, Seong K Kim, Young K Kim, Hang W Lee, Jee W Lee, Hye K Kwon, Tae S Jeong, Daejeon, Republic Of Korea assigned to Korea Research Institute of Chemical Technology

The present invention provides a novel micro- organism, Bacillus subtilis subspecies Kric- tiensis, and its mutant, Bacillus subtilis subspecies Krictiensis M 18-91; and a novel anti- fungal complex, KRF-001, produced from said microorganisms. The present invention also pro- vides a process for producing said complex by culturing or fermenting said microorganisms and a process for purifying said complex to a desired degree of high purity.

5155214

BASIC FIBROBLAST GROWTH FACTOR

Andrew J Baird, Frederick Esch, Denis Gospodarowicz, Pete Bohlen, Nicholas C Ling assigned to The Salk Institute for Biological Studies

PATENT ABSTRACTS

Substantially pure mammalian basic fibroblast growth factors are produced. The amino acid residue sequences of bovine and human bFGF are disclosed as well as aDNA chain encoding the polypeptide of the bovine species. By ap- propriately inserting a synthesized DNA chain into a cloning vector and using the cloning vector to transform cells, synthetic bFGF can be ob- tained from transformed cell lines, both prokaryotic and eukaryotic.

5155216

N U C L E I C A C I D S L A B E L E D W I T H A C H E M I L U M I N E S C E N T

A C R I D I N E E S T E R

Graham A Mock, Michael Septak, Michael J Powell assigned to Amoco Corporation

Polynucleotides are labeled with chemilumines- cent acridine esters or luminescent lanthanides. Labelling is preferably carried out by in- corporating a functional group into a nucleotide or polynucleotide at the C4 position of the pyrimidine portion or the C6 position of the purine portion and bonding a chemiluminescent acridine ester or a luminescent lanthanide to the functional group. The labeled polynucleotides are useful for direct detection of homologous polynucleotide sequences.

5155218

D N A E N C O D I N G H U M A N 5 - H T 1 D R E C E P T O R S

Richard L Weinshank, Theres Branchek, Paul R Hartig assigned to Neurogenetic Corporation

This invention provides isolated nucleic acid molecules encoding human 5-HT1D receptors, isolated proteins which are human 5-HT1D receptors, vectors comprising isolated nucleic acid molecules encoding human 5-HT1D recep- tors, mammalian cells comprising such vectors, antibodies directed to the human 5-HT1D recep- tors, nucleic acid probes useful for detecting nucleic acid encoding human 5-HT 1D receptors, antisense oligonucleotides complementary to any sequences of a nucleic acid molecule which encodes a human 5-HT1D receptor, pharma- ceutical compounds related to human 5-HT1D receptors, and nonhuman transgenic animals which express DNA a normal or a mutant human 5-HTID receptor. This invention further provides methods for determining ligand hi-

PATENT ABSTRACTS

nding, detecting expression, drug screening, and treatment involving the human 5-HTID recep- tor.

5156955

N U C L E O S I D E O X I D A S E A N D A S S A Y M E T H O D U T I L I Z I N G

S A M E

Yoshikaz Isono, Masami Hoshino, Otsu, Japan assigned to Otsuka Foods Co Ltd

The present invention provides a novel nucleoside oxidase YT-1 serving as an enzyme for catalyzing oxidation reaction ofa nucleoside, and oxidase being characterized in that the ox- idase causes the nucleoside to react with molecular oxygen to produce a nucleoside-5'- earboxylic acid via a nucleoside-5'-aldehyde without producing hydrogen peroxide, a process for producing the enzyme, a novel micro- organism having ability to produce the enzyme, and a novel analysis or assay method for nucleosides and the like utilizing the enzyme.

5156957

F O L L I C L E S T I M U L A T I N G H O R M O N E

Vemuri Reddy, Nancy Hsiung, Anton K Beck, Edward G Berstine assigned to Genzyme Cor- poration

Biologically active heterodimeric human FSH composed of an alpha subunit and a beta sub- unit, each subunit being synthesized by a cell having an expression vector containing hetero- Iogous DNA encoding the subunit.

133

membrane protein of Bordetella pertussis. The invention also relates to host cells and vectors comprising the nucleotide sequence, as well as a vaccine composition comprising the substan- tially pure protein.

5156959

M E T H O D T O E X P O R T G E N E P R O D U C T S T O T H E G R O W T H

M E D I U M O F G R A M N E G A T I V E B A C T E R I A

Lars Abrahmsen, Tomas Moks, Bjorn Nilsson, Mathias Uhlen, Stockholm, Sweden assigned to KabiGen AB

A process for expressing proteins in Gram(-) bacteria and providing for extracellular secre- tion thereof, comprising the steps: a) introducing into a Gram(-) bacterium a recombinant DNA construction comprising a promoter, a signal sequence enabling translocation and processing, and a structural gene encoding the desired pro- tein to be expressed; b) cultivating the bacterium under conditions resulting in filamentous growth; and c) recovering the extraceUularly secreted protein; a recombinant DNA construc- tion comprising: a promoter, a signal sequence and a structural gene including a cleavage re- gion, wherein the structural gene is of the formula: See Patent for Tabular Presentation PS where n is an integer ) or = 1, m is an integer, Y is a DNA sequence encoding a desired protein and E and B are the genes corresponding to the pro- tein A regions E and B, with the proviso that the structural gene is different from that encoding natural protein A; a plasmid vector comprising such recombinant DNA construction; a Gram(-) bacterium harbouring said recombinant DNA construction; and proteins obtained by such pro- cess.

5156958

G E N E E N C O D I N G A 30 K I L O D A L T O N O U T E R

MEMBRANE P R O T E I N O F B O R D E T E L L A P E R T U S S I S A N D

M E T H O D O F R E C O M B I N A N T P R O D U C T I O N O F S A I D P R O T E I N

Patricia A Reilly assigned to American Cyanamid Company

The present invention relates to a nucleotide and amino acid sequence of a 30 kilodalton outer

5156968

P U R I F I E D Y E A S T U B I Q U I T I N H Y D R O L A S E

Chung-Cheng Liu assigned to Genentech Inc

Ubiquitin hydrolase is provided having a purity of at least 70% homogeneity based on the weight of the total protein in the composition, which hydrolase hydrolyzes a ubiquitin-polypeptide conjugate at the amide bond linking the ubi- quitin and polypeptide, thereby yielding intact polypeptide with an unconjugated, mature N-