4987065 In vivo labelling of polynucleotide sequences

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PATENT moles of bilirubin and one mole of oxygen to yield two moles of biliverdin and two moles of water. A process is also provided for the produc- tion of said novel bilirubin oxidase, which com- prises cultivating a bilirubin oxidase-producing fungus of the genus Penicillium in a culture medium and collecting the bilirubin oxidase from the culture mixture. A method of deter- mining the content of bilirubin in a sample to be analyzed is further provided which comprises contacting the sample with the bilirubin oxidase to convert bilirubin in the sample into biliverdin and determining the amount of biliverdin for- med or the amount of oxygen consumed there- for, as well as a method for removing bilirubin contained in a sample to be analyzed, which comprises contacting the sample with said novel bilirubin oxidase. 4985363 MICROORGANISM CAPABLE OF GROWING IN 50% OR MORE ORGANIC SOLVENT Akira lnoue, Kouki Horikoshi, Tokyo, Japan assigned to Research Development Corporation New microorganisms belonging to Pseudo- monas putida or Pseudomonas sp., which are isolated from soil and have tolerance to one or more of hydrocarbons, alcohols, ethers, ketones and their derivatives or their mixture. These new microorganisms can be used in the fields of bio- reactor, liquid-waste treatment, protein engineering, etc. 4986916 METHOD OF MONITORING AND/OR CONTROLLING BIOLOGICALLY CATALYZED REACTIONS Robert Hickey assigned to New York State Energy Research and Development Authority In a method of monitoring and/or controlling a biologically catalyzed reaction, a trace gas which either is, or equilibrates with a metabolic inter- mediate is identified, and the concentration of the trace gas evolving from the reaction is measured. The measured concentration of trace gas is employed to evaluate the metabolic status of the reaction by a deterministic relationshhip, e.g. an empirical relationship or thermodynamic ABSTRACTS 4~ model. The deterministic relationship may in- volve other gaseous parameters of the reaction. Preferably, the measured concentration of the trace gas is converted via the deterministic rela- tionship into an estimate of the concentration of a different chemical component of interest for a particular application of the reaction. Carbon monoxide has been found to be a suitable trace gas for purposes of monitoring the metabolic state of the anaerobic digestion process. Meta- bolic state information derived on-line in ac- cordance with the present invention can be advantageously used to control the reaction. 4987065 IN VIVO I,ABELLING OF POLYNUCLEOTIDE SEQUENCES Jannis Stavrianopoulos. Heuy-Lan Yang. Nor- man E Kelker assigned to Enzo Biochem Inc In vivo labelled polynucleotides, processes for in vivo labelling of polynucleotides, and detection methods and kits characterized by those labelled polynucleotides. The in vivo on biologically- labelled polynucleotides of this invention are useful in the detection of various analytes and in other laboratory, industrial and medical applica- tions. 4987066 PROCESS FOR THE DETECTION OF RESTRICTION FRAGMENT LENGTH POLYMORPHISMS IN EUKARYOTIC GENOMES Jorg T Epplen, Freiburg, Federal Republic Of Germany assigned to Max Planck-Gesellschaft Zur Forderung der Wisscnschaften E V The invention is directed to a process for the analysis ofeukaryotic DNA wherein DNA ofan individual is isolated, the DNA is subjected to restriction endonuclease digest, the cleaved DNA is separated by gel electrophoresis, the gel is dried and hybridized with a probe, and the pat- tern of the DNA is evaluated. The process is characterized in that the probe used is an oligonucleotide which specifically hybridizes to eukaryotic simple tandem repeats having a periodicity of 2 to 4 bases. The process of the in- vention may be employed for the in vitro dia- gnosis of genetic defects such as hereditary diseases or for the determination of the genetic interrelationships.

Transcript of 4987065 In vivo labelling of polynucleotide sequences

Page 1: 4987065 In vivo labelling of polynucleotide sequences

PATENT

moles of bilirubin and one mole of oxygen to yield two moles of biliverdin and two moles of water. A process is also provided for the produc- tion of said novel bilirubin oxidase, which com- prises cultivating a bilirubin oxidase-producing fungus of the genus Penicillium in a culture medium and collecting the bilirubin oxidase from the culture mixture. A method of deter- mining the content of bilirubin in a sample to be analyzed is further provided which comprises contacting the sample with the bilirubin oxidase to convert bilirubin in the sample into biliverdin and determining the amount of biliverdin for- med or the amount of oxygen consumed there- for, as well as a method for removing bilirubin contained in a sample to be analyzed, which comprises contacting the sample with said novel bilirubin oxidase.

4985363

M I C R O O R G A N I S M C A P A B L E O F G R O W I N G IN 50% O R M O R E

O R G A N I C S O L V E N T

Akira lnoue, Kouki Horikoshi, Tokyo, Japan assigned to Research Development Corporation

New microorganisms belonging to Pseudo- monas putida or Pseudomonas sp., which are isolated from soil and have tolerance to one or more of hydrocarbons, alcohols, ethers, ketones and their derivatives or their mixture. These new microorganisms can be used in the fields of bio- reactor, liquid-waste treatment, protein engineering, etc.

4986916

M E T H O D O F M O N I T O R I N G A N D / O R C O N T R O L L I N G

B I O L O G I C A L L Y C A T A L Y Z E D R E A C T I O N S

Robert Hickey assigned to New York State Energy Research and Development Authority

In a method of monitoring and/or controlling a biologically catalyzed reaction, a trace gas which either is, or equilibrates with a metabolic inter- mediate is identified, and the concentration of the trace gas evolving from the reaction is measured. The measured concentration of trace gas is employed to evaluate the metabolic status of the reaction by a deterministic relationshhip, e.g. an empirical relationship or thermodynamic

ABSTRACTS 4~

model. The deterministic relationship may in- volve other gaseous parameters of the reaction. Preferably, the measured concentration of the trace gas is converted via the deterministic rela- tionship into an estimate of the concentration of a different chemical component of interest for a particular application of the reaction. Carbon monoxide has been found to be a suitable trace gas for purposes of monitoring the metabolic state of the anaerobic digestion process. Meta- bolic state information derived on-line in ac- cordance with the present invention can be advantageously used to control the reaction.

4987065

IN V I V O I , A B E L L I N G O F P O L Y N U C L E O T I D E S E Q U E N C E S

Jannis Stavrianopoulos. Heuy-Lan Yang. Nor- man E Kelker assigned to Enzo Biochem Inc

In vivo labelled polynucleotides, processes for in vivo labelling of polynucleotides, and detection methods and kits characterized by those labelled polynucleotides. The in vivo on biologically- labelled polynucleotides of this invention are useful in the detection of various analytes and in other laboratory, industrial and medical applica- tions.

4987066

P R O C E S S F O R T H E D E T E C T I O N O F R E S T R I C T I O N F R A G M E N T

L E N G T H P O L Y M O R P H I S M S IN E U K A R Y O T I C G E N O M E S

Jorg T Epplen, Freiburg, Federal Republic Of Germany assigned to Max Planck-Gesellschaft Zur Forderung der Wisscnschaften E V

The invention is directed to a process for the analysis ofeukaryotic DNA wherein DNA ofan individual is isolated, the DNA is subjected to restriction endonuclease digest, the cleaved DNA is separated by gel electrophoresis, the gel is dried and hybridized with a probe, and the pat- tern of the DNA is evaluated. The process is characterized in that the probe used is an oligonucleotide which specifically hybridizes to eukaryotic simple tandem repeats having a periodicity of 2 to 4 bases. The process of the in- vention may be employed for the in vitro dia- gnosis of genetic defects such as hereditary diseases or for the determination of the genetic interrelationships.