Building a morbidostat: an automated continuous-culture device for ...
4910146 Automated plant culture proliferation system
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PATENT ABSTRACTS continuously produced, with a yield of 80-90% and a volumetric productivity of 1.3 g/l.h. This represents a significant improvement in final concentration, yield and the volumetric pro- ductivity over the equivalent values of the cor- responding batch formentation, which were 18 g/l, 40% and 0.06 g/l.h, respectively. 4910146 AUTOMATED PLANT CULTURE PROLIFERATION SYSTEM Yossef Tur-Kaspa, Robert D Hartman, Ken- neth J Hornacek, Kevin J Brendel assigned to Hartmans Plants Inc An automated plant tissue proliferation system in which mature plants within a culture vessel are cut into a plurality of plugs using a die type cutter block. The cutter block is moved using a pick and place actuator to a work station where any extraneous nutrient media or plant material ad- hering to the sides of the cutter are removed using an indexed membrane sheet. A pair of op- posed articulated wiping arms cause the mem- brane sheet to be wrapped around the cutter and pulled down vertically along its sides to remove the excess material. Thereafter, the cutter is inde- xed to deposit some fraction of the cut cells into a container having culture medium. Fluid pressure is used to eject material from the appropriate cells onto the media material. A number of addi- tional containers are indexed into position with the cutter until all of the plugs of material are removed, the actuator and ejection system are so operated to distribute the plugs of plant material in a preselected array in a number of containers. The system provides rapid plant proliferation minimizing labor requirements and the liklihood of tissue culture contamination. 4910147 CELL CULTURE MEDIA FLEXIBLE CONTAINER David V Bacehowski, Arnold Bilstad, David Fisher, Rober Gliniecki, Michael R Keilman, Sidney T Smith assigned to Baxter International Inc A cell culture media container is provided. The container comprises a body constructed from flexible film and defining a containment area for containing the cell culture media, the body in- 623 cluding a front face and a back face, the front and back face being sealed to each other along at least three sides thereof. The container includes a fill port for filling the containment area with cell culture media, the port being sealed to a face of the body and being so constructed and arranged that it extends from the face normal thereto. The container is constructed from a high barrier, op- . tically clear, radiation sterilizabl¢ film. 4910148 MAGNETIC SEPARATION OF MAGNETIZED PARTICLES FROM BIOLOGICAL FLUIDS Ott Sorensen, Gunnar Kvalheim, Eivind Siem, Oslo, Norway assigned to Dynal A S A method and device are provided for separating magnetized particles from biological fluids. Cells such as cancer cells coated with magnetized particles can be separated from uncoated healthy cells. A fluid mixture of cancer cells, healthy cells and magnetizable particles is introduced into a container such as a disposable blood bag which is attached in a cassette on an underlying plane magnetic plane that provides a magnetic field. Incubation is carried out during which the cancer cells become coated with the magnetizable particles. The magnetic field pulls the coated cells down towards the bottom of the bag and anchors them, and uncoated healthy cells are removed from the bag. The separated uncoated healthy cells may be advanced through a final separation unit where any loose magnetizable particles are removed. There is provided means for adjusting vertical distance between the cassette and the magnetic plane and for agitating fluid within the container attached to the cassette. A magnetic screen plate may be placed between the cassette and the magnetic plate to short circuit the magnetic field during in- cubation. The screen plate is removed to carry out separation. 4910152 METHOD FOR BINDING OPIOID RECEPTORS Vera K Meyers, Ahmet Koman A method of controlling weight in a mammal and diagnosing, treating, and preventing dis- orders associated with delta-type opioid recep-
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Transcript of 4910146 Automated plant culture proliferation system
PATENT ABSTRACTS
continuously produced, with a yield of 80-90% and a volumetric productivity of 1.3 g/l.h. This represents a significant improvement in final concentration, yield and the volumetric pro- ductivity over the equivalent values of the cor- responding batch formentation, which were 18 g/l, 40% and 0.06 g/l.h, respectively.
4910146
A U T O M A T E D P L A N T C U L T U R E P R O L I F E R A T I O N S Y S T E M
Yossef Tur-Kaspa, Robert D Hartman, Ken- neth J Hornacek, Kevin J Brendel assigned to Hartmans Plants Inc
An automated plant tissue proliferation system in which mature plants within a culture vessel are cut into a plurality of plugs using a die type cutter block. The cutter block is moved using a pick and place actuator to a work station where any extraneous nutrient media or plant material ad- hering to the sides of the cutter are removed using an indexed membrane sheet. A pair of op- posed articulated wiping arms cause the mem- brane sheet to be wrapped around the cutter and pulled down vertically along its sides to remove the excess material. Thereafter, the cutter is inde- xed to deposit some fraction of the cut cells into a container having culture medium. Fluid pressure is used to eject material from the appropriate cells onto the media material. A number of addi- tional containers are indexed into position with the cutter until all of the plugs of material are removed, the actuator and ejection system are so operated to distribute the plugs of plant material in a preselected array in a number of containers. The system provides rapid plant proliferation minimizing labor requirements and the liklihood of tissue culture contamination.
4910147
C E L L C U L T U R E M E D I A F L E X I B L E C O N T A I N E R
David V Bacehowski, Arnold Bilstad, David Fisher, Rober Gliniecki, Michael R Keilman, Sidney T Smith assigned to Baxter International Inc
A cell culture media container is provided. The container comprises a body constructed from flexible film and defining a containment area for containing the cell culture media, the body in-
623
cluding a front face and a back face, the front and back face being sealed to each other along at least three sides thereof. The container includes a fill port for filling the containment area with cell culture media, the port being sealed to a face of the body and being so constructed and arranged that it extends from the face normal thereto. The container is constructed from a high barrier, op- . tically clear, radiation sterilizabl¢ film.
4910148
M A G N E T I C S E P A R A T I O N O F M A G N E T I Z E D P A R T I C L E S F R O M
B I O L O G I C A L F L U I D S
Ott Sorensen, Gunnar Kvalheim, Eivind Siem, Oslo, Norway assigned to Dynal A S
A method and device are provided for separating magnetized particles from biological fluids. Cells such as cancer cells coated with magnetized particles can be separated from uncoated healthy cells. A fluid mixture of cancer cells, healthy cells and magnetizable particles is introduced into a container such as a disposable blood bag which is attached in a cassette on an underlying plane magnetic plane that provides a magnetic field. Incubation is carried out during which the cancer cells become coated with the magnetizable particles. The magnetic field pulls the coated cells down towards the bottom of the bag and anchors them, and uncoated healthy cells are removed from the bag. The separated uncoated healthy cells may be advanced through a final separation unit where any loose magnetizable particles are removed. There is provided means for adjusting vertical distance between the cassette and the magnetic plane and for agitating fluid within the container attached to the cassette. A magnetic screen plate may be placed between the cassette and the magnetic plate to short circuit the magnetic field during in- cubation. The screen plate is removed to carry out separation.
4910152
M E T H O D F O R B I N D I N G O P I O I D R E C E P T O R S
Vera K Meyers, Ahmet Koman
A method of controlling weight in a mammal and diagnosing, treating, and preventing dis- orders associated with delta-type opioid recep-
