4766062 Displacement polynucleotide assay method and polynucleotide complex reagent therefor
Transcript of 4766062 Displacement polynucleotide assay method and polynucleotide complex reagent therefor
PATENT ABSTRACTS 63
4766062
D I S P L A C E M E N T P O L Y N U C L E O T I D E A S S A Y
M E T H O D A N D P O L Y N U C L E O T I D E C O M P L E X
R E A G E N T T H E R E F O R
Steven E Diamond, Joseph G Brewen, Jon I Wil- liams, Marian S Ellwood, Mary Collins, Edward F Fritsch assigned to Allied Corporation; Gene- tics Institute Inc
into the (P)/(L) binding region. A volume ex- cluding polymeric agent such as poly(ethylene oxide) (PEO or PEG) or other polyethers en- hances the rate of appearance of displaced labeled polynucleotide. Determination of dis- placed labeled polynucleotide (L) gives a value which is a function of the presence and con- centration of target nucleotide sequence (G) in the sample.
4766066
A diagnostic reagent is disclosed containing a complex of a probe polynucleotide (P) bound via purine/pyrimidine hydrogen bonding to a labeled polynucleotide (L). The probe (P) con- tains a target binding region (TBR) capable of binding to a target nucleotide sequence (G) of a biological sample. A method is disclosed in which contact with a sample containing the tar- get nucleotide sequence (G) causes binding, ini- tially between G and a single-stranded portion (IBR) of the target binding region (TBR). There- after the labeled polynucleotide (L) is displaced from the complex by branch migration of (G) into the (P)/(L) binding region. Determination of displaced labeled polynucleotide (L) gives a value which is a function of the presence and concentration of target nucleotide sequence (G) in the sample.
M E T H O D O F U S I N G B A C T E R I O P H A G E L A M B D A P1
P R O M O T E R T O P R O D U C E A F U N C T I O N A L P O L Y P E P T I D E I N
S T R E P T O M Y C E S
Stuart A Kuhstoss, R Nagaraja Rao assigned to Eli Lilly and Company
The invention relates to a method and cloning vehicle for the expression of a functional poly- peptide in Streptomyces. A recombinant DNA cloning vehicle was genetically engineered to bring the expression of the neomycin phosphotransferase gene under the control of the Escherichia coli bacteriophage lambdapL promoter.
4766064
D I S P L A C E M E N T P O L Y N U C L E O T I D E A S S A Y
E M P L O Y I N G P O L Y E T H E R A N D D I A G N O S T I C K I T
Jon I Williams, Marian S Ellwood, Mary Col- lins, Edward F Fritsch, Joseph G Brewen, Steven E Diamond assigned to Allied Corporation; Genetics Institute Inc
A diagnostic reagent is disclosed containing a complex of a probe polynucleotide (P) bound via purine/pyrimidine hydrogen bonding to a labeled polynucleotide (L). The probe (P) con- tains a target binding region (TBR) capable of binding to a target nucleotide sequence (G) of a biological sample. A method is disclosed in which contact with a sample containing the tar- get nucleotide sequence (G) causes binding, ini- tially between G and a single-stranded portion (IBR) of the target binding region (TBR). There- after the labeled polynucleotide (L) is displaced from the complex by branch migration of (G)
4766067
G E N E A M P L I F I C A T I O N
Debajit K Biswas assigned to President and Fel- lows of Harvard College
Hybrid DNA capable of effecting controlled amplification in a host cell of a heterologous gene that determines a specific function. The hybrid DNA has the following three different re- gions of DNA: (1) an amplicon that is inducible by an external stimulus; (2) the heterologous gene (or a site for its insertion); and (3) a selec- table marker enabling isolation of the transformed host cells. In order to make a desired compound coded by the heterologous gene, a vehicle including the hybrid DNA is in- troduced into a host cell is transformed with the vector, transformed host cells are grown to a desired population, the external stimulus is con- trolled to effect amplification of the gene in members of the population, cells containing am- plified gene are cultured, and the compound is recovered.