292 PATENT ABSTRACTS

A stable formulation of a non-cellulosic fabric having D-amino acid oxidase coated and dried thereon is shown. The fabric may be woven, non- woven, or knit and may be made from poly- olefin, nylon, polyester, or polyurethane fabrics. Polyolefin fabrics may be pretreated with a sur- factant. Also shown are formulations containing some or all of the reagents used to detect beta lac- tam antibiotics. Kits having webs coated with analytically effective amounts of D-amino acid oxidase, peroxidase, flavin adenine, dinucleotide, a peroxide sensitive dye and a D, D-carboxypeptidase substrate containing a car- boxyterminal D-alanine are shown. D,D- carboxypeptidase R39 enzyme may be provided separately in either aqueous form or dried on a separate piece of fabric. Under appropriate con- ditions the D,D-carboxypeptidase may also be dried on the fabric.

4753883

E N Z Y M E D E R E G U L A T I O N

Keith C Backman, Ramaswam Balakrishrlan as- signed to Biotechnica International Inc

Proteins having chorismate mutase-prephenate dehydratase (CMPD) activity, but lacking phenylalanine sensitivity are produced by gene- tic engineering. The proteins contain a sequence substantially corresponding to the N-terminal 337 amino acids of Eseherichia coli CMPD. Ex- pression vectors including genes coding for those proteins and regulatory DNA enabling their ex- pression are used to transform host micro- organisms, which are cultured to produce phenylalanine.

4756832

E N Z Y M A T I C P R O C E S S

Kenneth Gold, Bruce W Brodman assigned to The United States of America as represented by the Secretary of the Army

This invention is an improved enzymatic process for the removal of nitrite ions from an aqueous solution resulting from the denitration of a ni- trate ester. The aqueous solution is treated with an enzyme nitrite reductase resulting from in- ducement under faculatative anaerobic conditions of pseudomonas sp.

4758323

A S S A Y S Y S T E M S U S I N G M O R E T H A N O N E E N Z Y M E

Graham Davis, Hugh A Hill, Bedforshire, United Kingdom assigned to Genetics Interna- tional Inc

This specification discloses methods of detection or measurement of an enzyme or of its specific substrate, and sensors used in such methods. The present invention is concerned with a multi- enzyme system, and the specification discloses, as one aspect of the invention a method of assay in which an electrode poised at a suitable poten- tial is contacted with a system comprising a first enzyme, a cofactor linked with said enzyme and a mediator compound which transfers charge to the electrode from the first enzyme when its elec- trical state is changed by reaction of cofactor material. The cofactor may be NAD, NADP (both collectively referred to herein as NAD(P)), cAMP, ATP, GTP, TTP, or CTP. The specifica- tion particularly illustrates a method of assay in which an electrode (1) poised at a suitable poten- tial is contacted with a system comprising a first enzyme El a nicotinamide adenine dinucleotide compound N linked with said enzyme El and a mediator compound F which transfers charge to the electrodes from the first enzyme when its electrical state is changed by a NAD(P) NAD(P)H reaction. The NAD compound may act as a bridge between the said en- zyme/mediator system and further NAD utilizing enzyme E2.

4758508

A N A L Y T I C A L P R O C E S S A N D A G E N T S F O R T H E D E T E C T I O N

O F E S T E R O L Y T I C A N D / O R P R O T E O L Y T I C E N Z Y M E S

Eugen Schnabel, James Travis, A Christophe Skjold, Wuppertal, GA, Federal Republic Of Germany assigned to Miles lnc

Agent for the detection ofesterolytic and/or pro- teolytic enzymes, containing (a) an amino acid ester or peptide ester of a phenol, as the chromogenic enzyme substrate, and (b) an