92 PATENT

mechanism for stirring an egg white solution and an ion-exchange resin, having a strainer pro- vided in a lower portion thereof, having liquid discharge pipes connected thereto at a position below the strainer, preferably at the bottom of the tank, and at the side wall above the strainer at a height such that the resin when left at rest will not overflow, respectively, and further having a resin discharge pipe connected thereto at the lower portion of the side wall, above and in the vicinity of the strainer; a positive displace- ment pump connected to the liquid discharge pipes for transferring the egg white solution after removal of lysozyme to a reservoir tank; and a centrifugal pump connected to the resin dis- charge pipe for transferring the mixture of the resin and water to a separate tank, whereby col- lection by adsorption can be made simple, con- venient for use and also suitable for a small- quantity treatment.

ABSTRACTS

4713328

M I C R O B I A L E N Z Y M E A S S A Y S

Robert Yolken

A method is disclosed for efficient, rapid dia- gnosis of bacterial infections by measuring for the presence of beta-lactamase using 14C- benzylpenicillin as an enzyme substrate and isolating cleaved substrate with an ion exchange column.

4713344

H A N D O P E R A T E D P R E S S F O R T I S S U E E X T R A C T I O N

4713327

D E T E R M I N A T I O N O F T O T A L C R E A T I N E K I N A S E O R A N

I S O E N Z Y M E W I T H A M U L T I L A Y E R A N A L Y T I C A L

E L E M E N T

John B Findlay, Annie Wu, Gary E Norton as- signed to Eastman Kodak Company

A multilayer analytical element for the deter- mination ofcreatine kinase comprises a support having thereon, in order and in fluid contact, a registration layer and an isotropically porous spreading layer. The registration layer contains a binder material at a coverage of at least about 8 g/m2, which coverage improves the stability and keeping properties of the element in high humidity environments. This element is useful in an analytical method for determinin~ total creatine kinase or any one of its isoenzymes, e.g. creatine kinase-MB.

Albert Markhart assigned to Regents of the University of Minnesota

A hand operated press is used for extracting cell sap from biological tissue, such as from leaf tis- sue of plants, as well as from animal tissue as a removable barrel that holds a suitable screen in place in a well. The barrel houses a plunger that is of small diameter so that when the barrel has tissue added to it the plunger is inserted and a substantial pressure may be exerted manually to insure rupturing the cell membranes and releasing all of the cell liquid (in plants called sap) for analysis. In the preferred form the screen holds at least one standard size filter paper disk in place so that the liquid is first forced through the screen to retain large solid particles, and the filter paper disk is wetted for use in a standard analyzer for direct analysis of the desired charac- teristics. The press is portable, easy to use and disassemble for cleaning, and reduces the time required for determining characteristics of a cell, such as its osmotic potential, and also reduces the chance of the sap becoming altered by en- vironmental factors.

4713345

F E R M E N T A T I O N M E T H O D S A N D A P P A R A T U S

Steven L Ramsden assigned to Grain Processing Corporation