PATENT ABSTRACTS

human T cells and cutaneous T lymphoma cells. The hybrid is formed by fusing splenocytes from immunized CAF1 mice with P3X63Ag8UI myeloma cells. Diagnostic and therapeutic uses of the monoclonal antibody are also disclosed.

4654298

M E T H O D F O R D E T E C T I O N O F P E R I T O N E A L I N F L A M M A T I O N

O R I N F E C T I O N

James L Babb, Timothy G Bloomster, Jon A Rudbach assigned to Abbott Laboratories

Peritoneal inflammation or infection can be detected in a patient by assaying a peritoneal lavage sample from the patient for lysosomal en- zymes. This is done by combining the lavage sample with a leukocyte lysing agent and a chromogenic or fluorogenic enzyme substrate specific for lysosomal enzymes. The lysosomal enzymes can then be measured, and the in- flammation or infection detected, by measuring the color or fluorescence developed in the sample by action of the enzymes on the substrate.

4654301

P R O C E S S F O R D E T E C T I N G L D H K I S O Z Y M E A C T I V I T Y IN

H U M A N S E R U M F O R U S E A S A D I A G N O S T I C A I D A N D F O R

M O N I T O R I N G R E S P O N S E T O C A N C E R T H E R A P Y

Garth R Anderson, Kenneth F Manly, Arnold Mittelman assigned to Health Research lnc (Roswell Park Division)

An improved assay procedure for quantitatively detecting LDHk isozyme in human serum is pro- vided which utilizes previously known elec- trophoretic gel separation procedures combined with an improved staining method wherein the staining solution has a constant temperature of about 37£20 +0 C, and a pH of about 8.0, and is contacted with the gel matrix for at least 3 hours in the absence of oxygen and light. This im- proved assay procedure may be used as an adjunct to other procedures for diagnosing the presence of primary cancer, as a post-operative indicator of metastatic cancer and in monitoring the success of cancer therapy. + RE + RE. + RE

367

4654303

C O N S T R U C T I O N O F N O V E L M U T A N T M I C R O O R G A N I S M S

Scott Hagedorn assigned to Celanese Corpora- tion

This invention provides novel mutant strains of microorganisms (e.g., Pseudomonas putida Bio- type A) which are capable of converting sub- strates such as toluene, p-xylene, catechol and 4- methylcatechol to 2-hydroxymuconic semialdehyde or substituted analog of 2- hydroxymuconic semialdehyde quantitatively by the meta (catechol 2,3-oxygenase) pathway. No active 2-hydroxymuconic semialdehyde- metabolizing enzymes are induced in the microorganism, thereby permitting a 2- hydroxymuconic semialdehyde type metabolite to be produced and accumulated in a bioconver- sion medium containing the microorganism.

4657383

O P T I C A L A S S A Y M E T H O D F O R S T O R E D H U M A N P L A T E L E T S

Brian J Bellhouse, lslip, Oxfordshire, United Kingdom

The viability of a pack of stored blood platelets is monitored by gripping the pack between two plates (6) and (7) which are closed to pinch together the walls of the bag (10) along an L- shaped seal (14), leaving a channel (17), squeezing a part 05) of the bag by means of a reciprocating plunger (19), so that the platelets continually flow to and fro between the part (l 5) and the part (16), through the channel (17), and passing a beam of light from an LED (27) through the channel (17). to a photoresistor (29). The AC signal from the photoresistor cor- responds to the fluctuations in the intensity of the light passing through the channel 07) and the amplitude of this signal is representative of the viability, and hence the clinical acceptability, of the platelets.

4657760

M E T H O D S A N D C O M P O S I T I O N S U S I N G M O N O C L O N A L

A N T I B O D Y T O H U M A N T C E L L S

Patrick C Kung, Gideo Goldstein assigned to Ortho Pharmaceutical Corporation