4610 Outline
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MBIO 4610 MOLECULAR GENETICS OF EUKARYOTES Sept. -Dec. 2012 Instructor: Dr. Deborah Court; Office: 301A Buller Bldg (in the back of Lab 301) Contact: 474-8263 E-mail: [email protected] D2L (Angel replacement) Course Website Link – at https://universityofmanitoba.desire2learn.com/ Office Hours: Usually 1:30 - 3:00 pm Mon. You are welcome to try at other times or e-mail. Lab: Dr. L. Cameron, 414 Buller; 474-6452; email: [email protected] http://umanitoba.ca/science/micro300400labs/60_461.htm Assessment for final grade in the course: Laboratory 20% Quiz #1 Thurs. Oct. 11, 2012 12% Quiz #2 Tues. Nov. 13, 2012 18% Final Exam (Set by student records office) 50% TOPICS Reference Texts: A specific textbook is not required for this course. Molecular Biology by Weaver is an optional book this year; there is an older edition on one-hour reserve at the reference desk in the Science and Technology library. In addition, basic information can be found in any of the Genes books by Lewin (Genes V through IX; several copies are on reserve at the reference desk in the Science and Technology Library). The course notes (without diagrams), assignments and handouts, and where possible, links to research articles and images presented in class are provided on the D2L site. This outline may be modified; students are responsible for the material given in lectures and any additional reading assignments given in class. I. Basic methods in molecular genetics A. Gel electrophoresis, blotting and detection B. PCR II. Eukaryotic cells and their genes A. Organellar genomes – in mitochondria and chloroplasts – structure, coding content B. Nuclear genomes – overview of structure, coding and non-coding content III. Eukaryotic gene expression A. Transcription - RNA polymerases and basal promoters B. Regulation of transcriptional initiation C. RNA processing- splicing, polyadenylation D. Microarrays – assessing TS of many genes E. RNA editing F. Translation and its regulation IV. Mobile genetic elements A. Transposons B. Retrotransposons C. Highjacked transposases – Immunoglobulin gene rearrangements V. Current topics in Eukaryotic Molecular Genetics A. RNA interference RNAi (tentatively Dr. Whyard, Biological Sciences) B. Other topics if time permits
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Transcript of 4610 Outline
MBIO 4610 MOLECULAR GENETICS OF EUKARYOTES Sept. -Dec. 2012 Instructor: Dr. Deborah Court; Office: 301A Buller Bldg (in the back of Lab 301) Contact: 474-8263 E-mail: [email protected] D2L (Angel replacement) Course Website Link – at https://universityofmanitoba.desire2learn.com/ Office Hours: Usually 1:30 - 3:00 pm Mon. You are welcome to try at other times or e-mail. Lab: Dr. L. Cameron, 414 Buller; 474-6452; email: [email protected] http://umanitoba.ca/science/micro300400labs/60_461.htm Assessment for final grade in the course: Laboratory 20% Quiz #1 Thurs. Oct. 11, 2012 12% Quiz #2 Tues. Nov. 13, 2012 18% Final Exam (Set by student records office) 50%
TOPICS
Reference Texts: A specific textbook is not required for this course. Molecular Biology by Weaver is an optional book this year; there is an older edition on one-hour reserve at the reference desk in the Science and Technology library. In addition, basic information can be found in any of the Genes books by Lewin (Genes V through IX; several copies are on reserve at the reference desk in the Science and Technology Library). The course notes (without diagrams), assignments and handouts, and where possible, links to research articles and images presented in class are provided on the D2L site. This outline may be modified; students are responsible for the material given in lectures and any additional reading assignments given in class. I. Basic methods in molecular genetics A. Gel electrophoresis, blotting and detection B. PCR II. Eukaryotic cells and their genes A. Organellar genomes – in mitochondria and chloroplasts – structure, coding content B. Nuclear genomes – overview of structure, coding and non-coding content III. Eukaryotic gene expression A. Transcription - RNA polymerases and basal promoters B. Regulation of transcriptional initiation C. RNA processing- splicing, polyadenylation D. Microarrays – assessing TS of many genes E. RNA editing F. Translation and its regulation IV. Mobile genetic elements A. Transposons B. Retrotransposons C. Highjacked transposases – Immunoglobulin gene rearrangements V. Current topics in Eukaryotic Molecular Genetics A. RNA interference RNAi (tentatively Dr. Whyard, Biological Sciences) B. Other topics if time permits
