4411999 Immobilization of enzymes on granular gelatin

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332 PATENT ABSTRACTS practice, a site-deactivating medium such as an animal- or vegetable-derived total biological fluid or extract (e.g., plasma or gelatin) is cova- lently bound to the vessel wall surfaces for deac- tivation purposes. In preferred forms the process is solid-state, wherein one of the components of the antigen-antibody reaction is coupled cova- lently to the coating medium, and a reaction mix- ture fraction including the sample being determined and the other component which has been labeled, for instance by means of a colorimetfically active enzyme. 4414323 METHOD FOR MEASURING TRACE ENZYME Nobuhito Masuda, Minami ashigara, Japan as- signed to Fuji Photo Film Co Ltd In a photochemical measurement methodof a trace enzyme which comprises: using a synthetic substrate bringing either the reaction product formed by enzyme reaction or the unreacted syn- thetic substrate into contact with silver halide, developing the same, and measuring optical density of the formed silver image and/or colored dye, the synthetic substrate comprises at least one structure (A) which is specifically con- tacted with an enzyme to be measured and at least one photographically fogging agent struc- ture (B) in the molecule thereof. 4412~1 ISOLATION OF BACTERIAL LUCIFERASE Thomas O Baldwin, Thomas F Holzman as- signed to Board of Trustees of The University of Illinois Itl ! t~H~ y--C--Z I 1112 The present invention provides a novel means for the isolation of bacterial luciferase and a novel affinity resin useful in said isolation. 4411999 IMMOBILIZATION OF ENZYMES ON GRANULAR GELATIN Shigeki Shigesada, Hironoshi Kitagawa, Toshio Mihara, Yoshiaki lshimatsu, Machida, Japan assigned to Denki Kagaku Kogyo Kabushiki Kaisha A process for producing an immobilized enzyme composition comprises simultaneously reacting a non-proteolytic enzyme and a water soluble- multifunctional reagent with a non-hardened granular gelatin in an aqueous medium wherein the reacting is carried out in the presence of a water-soluble protein polymer compound in an amount of from 0.01 to 2 parts by weight relative to one part of the non-proteolytic enzyme. The non-proteolytic enzyme forms a uniform film on the surface of the granular gelatin and the bond between the non-proteolytic enzyme and the granular gelatin is strengthened by the water soluble protein polymer. 4411~9 PROCESSES AND DEVICES FOR DETECTION OF SUBSTANCES SUCH AS ENZYME INHIBITORS Ann E Grow assigned to Midwest Research In- stitute The disclosed methods and devices for detecting or monitoring or identifying substances (such as chemical warfare agents) and residual environ- mental pollutants (such as pesticides) utilize the discovery that spectra (e.g. infrared absorption spectra) of an uninhibited enzyme (e.g., a cholinesterase) can differ from spectra of the same enzyme which has been complexed with the agent pollutant. For example, the infrared spectrum of uninhibited butyrylcholinesterase (BuChE) lacks a distinct absorption peak found

Transcript of 4411999 Immobilization of enzymes on granular gelatin

Page 1: 4411999 Immobilization of enzymes on granular gelatin

332 PATENT ABSTRACTS

practice, a site-deactivating medium such as an animal- or vegetable-derived total biological fluid or extract (e.g., plasma or gelatin) is cova- lently bound to the vessel wall surfaces for deac- tivation purposes. In preferred forms the process is solid-state, wherein one of the components of the antigen-antibody reaction is coupled cova- lently to the coating medium, and a reaction mix- ture fraction including the sample being determined and the other component which has been labeled, for instance by means of a colorimetfically active enzyme.

4414323

M E T H O D F O R M E A S U R I N G T R A C E E N Z Y M E

Nobuhito Masuda, Minami ashigara, Japan as- signed to Fuji Photo Film Co Ltd

In a photochemical measurement methodof a trace enzyme which comprises: using a synthetic substrate bringing either the reaction product formed by enzyme reaction or the unreacted syn- thetic substrate into contact with silver halide, developing the same, and measuring optical density of the formed silver image and/or colored dye, the synthetic substrate comprises at least one structure (A) which is specifically con- tacted with an enzyme to be measured and at least one photographically fogging agent struc- ture (B) in the molecule thereof.

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I S O L A T I O N O F B A C T E R I A L L U C I F E R A S E

Thomas O Baldwin, Thomas F Holzman as- signed to Board of Trustees of The University of Illinois

I t l ! t~H~

y - -C- -Z I

1112

The present invention provides a novel means for the isolation of bacterial luciferase and a novel affinity resin useful in said isolation.

4411999

I M M O B I L I Z A T I O N O F E N Z Y M E S O N G R A N U L A R G E L A T I N

Shigeki Shigesada, Hironoshi Kitagawa, Toshio Mihara, Yoshiaki lshimatsu, Machida, Japan assigned to Denki Kagaku Kogyo Kabushiki Kaisha

A process for producing an immobilized enzyme composition comprises simultaneously reacting a non-proteolytic enzyme and a water soluble- multifunctional reagent with a non-hardened granular gelatin in an aqueous medium wherein the reacting is carried out in the presence of a water-soluble protein polymer compound in an amount of from 0.01 to 2 parts by weight relative to one part of the non-proteolytic enzyme. The non-proteolytic enzyme forms a uniform film on the surface of the granular gelatin and the bond between the non-proteolytic enzyme and the granular gelatin is strengthened by the water soluble protein polymer.

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P R O C E S S E S A N D D E V I C E S F O R D E T E C T I O N O F S U B S T A N C E S

S U C H A S E N Z Y M E I N H I B I T O R S

Ann E Grow assigned to Midwest Research In- stitute

The disclosed methods and devices for detecting or monitoring or identifying substances (such as chemical warfare agents) and residual environ- mental pollutants (such as pesticides) utilize the discovery that spectra (e.g. infrared absorption spectra) of an uninhibited enzyme (e.g., a cholinesterase) can differ from spectra of the same enzyme which has been complexed with the agent pollutant. For example, the infrared spectrum of uninhibited butyrylcholinesterase (BuChE) lacks a distinct absorption peak found