PATENT ABSTRACTS 315

infrared spectroscopy) and its spectrum com- pared to an uninhibited enzyme spectrum. Rela- tively simple devices can carry out the collection and detection or monitoring or identification steps of this invention, given appropriate models of complexed and uncomplexed enzyme specta upon which to base the design of the devices.

charcoal-adsorbable protein from Mycobac- terium tuberculosis which has a molecular weight of 20,000-30, 000 and which is immuno- chemically stable from 4 degrees0 C. to 250 degrees C. and has a pH range from 3.0 to 9.0. The method is particularly applicable to the detection of infectious tuberculosis in humans and determining the antibiotic sensitivity of in- fecting Mycobacteria.

4411988

P R O C E S S F O R T H E D E T E R M I N A T I O N O F A N T I - H Y A L U R O N I D A S E A N D AN

A G E N T S U I T A B L E F O R T H I S P U R P O S E

Tibor Toth, Reiner Gils, Marburg, Federal Republic Of Germany assigned to Behringwerke A G

A simple rapid assay for the determination of antihyaluronidase and a kit for use therein. The assay is based on the precipitation of unreacted hyaluronic acid by a cationic detergent and acid dyestuff. The assay is preferably performed as a dilution series. The dyestuffreagent contains I to l0 parts by weight acid dyestuff to 10 parts by weight of cationic detergent.

4410660

B I N D I N G A S S A Y F O R T H E D E T E C T I O N O F M Y C O B A C T E R I A

Eugene W Straus assigned to Montefiore Medical Center

A method for detecting the presence of Mycobacteria in a fluid or tissue which com- prises mixing the fluid or tissue containing a secretory product of Mycobacteria with a com- plex of a tracer-containing molecule and a bi- nding macromolecule having reversible binding affinity for the tracer-containing molecule and detecting the tracer-containing molecule, wherein the tracer-containing molecule is a

4410506

I M M U N O A S S A Y F O R V I T A M I N K - D E P E N D E N T B O N E P R O T E I N

Paul A Price, Satoru K Nishimoto assigned to The Regents of the University of California

lmmunoassay and compositions therefore for detection of human vitamin K-dependent bone protein. Heterologous labeled vitamin K- dependent protein or antigenic fragment thereof is employed with antibodies to the protein in an assay for the protein in various physiological fluids for bone extracts. The assay may be used in the diagnosis of bone diseases, by itself or in con- junction with an alkaline phosphatase assay.

4409335

M E T H O D F O R E L I M I N A T I N G G L U C O S E D E P E N D E N T S C H I F F

B A S E E F F E C T F R O M H E M O G L O B I N A I A S S A Y

Mark S Hanamoto, Steve K Tanaka assigned to Bio-Rad Laboratories lnc

An ion exchange method for the separation of hemoglobin Al from its Schiff base precursors and from nonglycosylated hemoglobin in a sam- ple of human blood. The known method of lysing the sample, using it to impregnate a weak cation exchange resin, eluting out the glycosylated components with a buffer solution containing from about 0.6 M to about 0. ! 1 M alkali metal ion dissolved therein, and recovering the eluate, is modified by the inclu- sion ofa dihydroxyboryl compound in either the hemolysate, the elution buffer, or both.