4. Gene-terpenoid networks
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v v v Jingrui Li 1 , Yanmei Dong 1 , Wenying Zhang 1 , Di Wang 1 , Hongtong Bai 1 , Hui Li 1, *, Lei Shi 1, * 1 Key Laboratory of Plant Resources and Beijing Botanical Garden, Institute of Botany, Chinese Academy of Sciences, Xiangshan, Beijing 100093, China. *Corresponding author. Email: [email protected] for Lei Shi; [email protected] for Hui Li. Lavandula is a distinctive genus that belongs to the species-rich and chemically diverse subclade Nepetoideae (3600 species) within Lamiaceae (~7173 species). The aromatic shrub L. angustifolia produces various volatile terpenoids that serve as resources for essential oils and function in plant- insect communication, such as in herbivore deterrence or pollinator attraction. To better understand the genetic basis of the terpenoid diversity in lavender, we produced a chromosome-level lavender referenced genome using the latest sequencing technologies and bioinformatic methods, investigated the duplicates resulting from the two last WGDs and tandem repeats, and identified the candidate genes closely correlated to the primary attractive and defensive compounds. Comprehensive analysis of the genetic basis of the production of volatiles in lavender could serve as a foundation for future research into lavender evolution, phytochemistry, and ecology. 1. Chromosome-level assembly of the lavender genome 3. Identification of lavender volatile terpenoids and their biosynthetic genes 4. Gene-terpenoid networks High-quality assembly of the 894.5 Mb lavender genome sequence distributed across 27 chromosomes; Lavender has exclusively undergone two WGDs, allowing adaptation to the Mediterranean environment; Gene duplications due to WGDs and tandem duplications led to terpenoid diversification. This work was supported by the Strategic Priority Research Program of the Chinese Academy of Sciences (Grant No. XDA23080603) and the National Natural Science Foundation of China (Grant No. 31701956). Table 1 Summary of tandem duplications in lavender and four other Lamiaceae species. Figure 2 Phylogeny of lavender. 2. Lineage-specific whole-genome duplication (WGD) and tandem duplication of lavender Figure 3 The sites, types, contents, and putative functions of volatile production in lavender. Figure 4 Biosynthesis of volatile terpenoid in lavender. Figure 5 Gene-terpenoid network of candidates strongly associated with attractive and defensive terpenoids in lavender. Figure 6 Transcriptional changes in representative genes in clusters in response to methyl jasmonate or during flower development. Figure 1 Genomic landscape of lavender. Smil, Salvia miltiorrhiza; Sspl, Salvia splendens; Sbai, Scutellaria baicalensis; Tgra, Tectona grandis.
Transcript of 4. Gene-terpenoid networks
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Jingrui Li1, Yanmei Dong1, Wenying Zhang1, Di Wang1, Hongtong Bai1, Hui Li1,*, Lei Shi1,*1Key Laboratory of Plant Resources and Beijing Botanical Garden, Institute of Botany, Chinese Academy of Sciences, Xiangshan, Beijing 100093, China. *Corresponding author. Email: [email protected] for Lei Shi; [email protected] for Hui Li.
Lavandula is a distinctive genus that belongs to the species-rich and chemically diverse subclade Nepetoideae (3600 species) within Lamiaceae (~7173 species). The aromatic shrub L. angustifolia produces various volatile terpenoids that serve as resources for essential oils and function in plant-insect communication, such as in herbivore deterrence or pollinator attraction.To better understand the genetic basis of the terpenoid diversity in lavender, we produced a chromosome-level lavender referenced genome using the latest sequencing technologies and bioinformatic methods, investigated the duplicates resulting from the two last WGDs and tandem repeats, and identified the candidate genes closely correlated to the primary attractive and defensive compounds. Comprehensive analysis of the genetic basis of the production of volatiles in lavender could serve as a foundation for future research into lavender evolution, phytochemistry, and ecology.
1. Chromosome-level assembly of the lavender genome
3. Identification of lavender volatile terpenoids and their biosynthetic genes
4. Gene-terpenoid networks
High-quality assembly of the 894.5 Mb lavender genome sequence distributed across 27 chromosomes;
Lavender has exclusively undergone two WGDs, allowing adaptation to the Mediterranean environment;
Gene duplications due to WGDs and tandem duplications led to terpenoid diversification.
This work was supported by the Strategic Priority Research Program of the Chinese Academy of Sciences (Grant No. XDA23080603) and the National Natural Science Foundation of China (Grant No. 31701956).
Table 1 Summary of tandem duplications in lavender and four other Lamiaceae species.
Figure 2 Phylogeny of lavender.
2. Lineage-specific whole-genome duplication (WGD) and tandem duplication of lavender
Figure 3 The sites, types, contents, and putative functions of volatile production in lavender.
Figure 4 Biosynthesis of volatile terpenoid in lavender.
Figure 5 Gene-terpenoid network of candidates strongly associated with attractive and defensive terpenoids in lavender.
Figure 6 Transcriptional changes in representative genes in clusters in response to methyl jasmonate or
during flower development.
Figure 1 Genomic landscape of lavender.
Smil, Salvia miltiorrhiza; Sspl, Salvia splendens; Sbai, Scutellaria baicalensis; Tgra, Tectona grandis.
