4/7/2015

1

Photo citations: 1. x

Metastasis Part 1

1.Cell Migration

2.PAR 1, an Outside-

in-Signal

3.Signal Transduction

intermediates

4.PAR 1 Localization

via antibody-QD

Objectives

Learn about cell signaling from the perspective of a

G-protein coupled receptor (GPCR).

Understand the signals that stimulate these GPCRs

and the downstream effects of GPCR stimulation.

Develop a deep understanding of what an abstract is.

Understand antibody-based techniqueS (numerous)

for localizing proteins of interest and recognizing

which is important for a given purpose.

Photo citations: 1. Karp, figure 9.70 2. Karp, figure 9.69.

Metastasis Part 1

1.Cell Migration

2.PAR 1, an Outside-

in-Signal

3.Signal Transduction

intermediates

4.PAR 1 Localization

via antibody-QD

Photo citations: 1. Karp, figure 9.1

Metastasis Part 1

1.Cell Migration

2.PAR 1, an Outside-

in-Signal

3.Signal Transduction

intermediates

4.PAR 1 Localization

via antibody-QD

4/7/2015

2

Photo citations: 1. Karp, figure 9.64

Metastasis Part 1

1.Cell Migration

2.PAR 1, an Outside-

in-Signal

3.Signal Transduction

intermediates

4.PAR 1 Localization

via antibody-QD

Photo citations: 1. Karp, figure 9.70

Metastasis Part 1

1.Cell Migration

2.PAR 1, an Outside-

in-Signal

3.Signal Transduction

intermediates

4.PAR 1 Localization

via antibody-QD

Photo citations: 1. Creative commons

File:A2A_receptor_bilayer.png

Metastasis Part 1

1.Cell Migration

2.PAR 1, an Outside-

in-Signal

3.Signal Transduction

intermediates

4.PAR 1 Localization

via antibody-QD

PAR1 = protease activated receptor

G-protein coupled receptor

4/7/2015

3

Photo citations: van der Poll Critical Care 2008 12(Suppl 6):S3 doi:10.1186/cc7026

Metastasis Part 1

1.Cell Migration

2.PAR 1, an Outside-

in-Signal

3.Signal Transduction

intermediates

4.PAR 1 Localization

via antibody-QD

Photo citations: 1. Karp, Figure 15.4

Metastasis Part 1

1.Cell Migration

2.PAR 1, an Outside-

in-Signal

3.Signal Transduction

intermediates

4.PAR 1 Localization

via antibody-QD

Photo citations: 1. x

Metastasis Part 1

1.Cell Migration

2.PAR 1, an Outside-

in-Signal

3.Signal Transduction

intermediates

4.PAR 1 Localization

via antibody-QD

http://www.youtube.com/w

atch?v=xT0mAQ4726s

4/7/2015

4

Photo citations: 1. Karp, figure 15.2

Metastasis Part 1

1.Cell Migration

2.PAR 1, an Outside-

in-Signal

3.Signal Transduction

intermediates

4.PAR 1 Localization

via antibody-QD

Photo citations: 1. Karp, figure 15.7

Metastasis Part 1

1.Cell Migration

2.PAR 1, an Outside-

in-Signal

3.Signal Transduction

intermediates

4.PAR 1 Localization

via antibody-QD

Photo citations: 1. Karp, Figure 9.69

Metastasis Part 1

1.Cell Migration

2.PAR 1, an Outside-

in-Signal

3.Signal Transduction

intermediates

4.PAR 1 Localization

via antibody-QD

4/7/2015

5

Photo citations: 1. imgarcade.com 2. http://commons.wikimedia.org/wiki/File:C

dSeqdots.jpg 3. http://commons.wikimedia.org/wiki/File:Bi

ological_and_technological_scales_compared-en.svg

Metastasis Part 1

1.Cell Migration

2.PAR 1, an Outside-

in-Signal

3.Signal Transduction

intermediates

4.PAR 1 Localization

via antibody-QD

Photo citations: Image Credit: Quantum Dot Corporation ://nanotechweb.org/cws/article/tech/16533

Metastasis Part 1

1.Cell Migration

2.PAR 1, an Outside-

in-Signal

3.Signal Transduction

intermediates

4.PAR 1 Localization

via antibody-QD