Tumors  evolve   from   single   cells.     As   they  evolve   they   acquire   complex  genomic  mutations   and  diverge   to   form   distinct   clonal   lineages   and   subpopulations,   resulting   in   intratumor  heterogeneity.    This  genomic  heterogeneity  may  play  an  important  role  in  tumor  growth,  during  complex  biological  processes   such  as   invasion,  metastasis   and   therapy  resistance.     In   this   talk   I  will  provide  an  overview  of  the  experimental  technologies  and  computational  methods  that  our  group  has  developed  for  performing  single  cell  DNA  sequencing  and  analyzing  the  resulting  large-­‐scale  datasets.    I  will  also  discuss  our  efforts  in  applying  these  methods  to  study  punctuated  copy  number   evolution   in   triple-­‐negative   breast   cancer   patients   and   metastatic   dissemination   in  colorectal  cancers.      

Tumor  Evolution  at  Single  Cell  Genomic  Resolution  

                               Monday,  March  27,  2017                                            4:15  PM  –  5:15  PM    

127  Hayes-­‐Healy  Center   Colloquium Tea 3:45 PM to 4:15 PM 154 Hurley Hall

Nicholas  Navin  Department  of  Genetics  

MD  Anderson  Cancer  Center    

Department  of  Applied  and  Computational    Mathematics  and  Statistics  Colloquium