30 Jun Microsopy
Transcript of 30 Jun Microsopy
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Microscopy
What is a microscope?
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Microscope
Microscope is a toolwhich can help you seetiny objects and livingorganisms. It makesthem look bigger.
This ability of the
microscope is called itsmagnifying power ormagnication.
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Light microscope
Light microscope uses diused light from thesun or articial light to illuminate the object tobe observed.
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Microscopy
What are the parts of the microscope?
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1.Arm
upports the body tube.
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2. Base
upports and stabili!es themicroscope.
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3. Eyepiece / ocular lens
Magnies image producedby objective lens.
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4. Body Tube
Maintains the properdistance between the
objective and ocular
lenses.
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Moves stage up and downa large amount for
"#$%I&'.
5. Coarse adjustment knob
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Moves stage up and downa tiny amount to
()*+,& the image.
6. Fine adjustmentknob
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7. Light source
-lamp or mirror +rovideslight for viewing the slide.
+rojects light %+/)*0
through the diaphragm1
the +,$IM,&1 and
the L,&,.
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8. Diaphragm
$ontrols the amount oflight passing through
the slide.
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tage clips2 hold the slide in place.
tage
2 upports the slide
being viewed.
9. Stage and stage clips
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10. Objective lenses
"ocus and magnifylight coming through the
slide.
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*otates to allow use of dierent power objectives.
11. Revolving nosepiece
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upports the arm andcontrols the body of the
microscope.
12. Inclination joint
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Follow these instructions when using the
microscope.
1. To carry the microscope, grasp the
ARM with one hand. Hold the BASE withyour other hand.
Using the Microscope in 10 Steps
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34 teps to
%sing the Microscope. Ad!ust the "#A$HRA%M while loo&ing
through the E'E$#E(E.
). Ad!ust the *#%HT S+R(E -mirror until
you see the circle o/ light.
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34 teps to%sing the Microscope
0. $lace the slide on the STA%E.
. (enter the S$E(#ME2 o3er the small opening on the STA%E.
4. Secure the slide with the STA%E (*#$S.
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34 teps to%sing the Microscope
5. 6hile loo&ing at the STA%E /rom the side,
turn the (+RSE F+(S 72+B to lower
the B+"' TBE until the *+6 $+6ER +B8E(T#9E almost touches the slide.
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34 teps to%sing the Microscope
:. 6hile loo&ing through the E'E$#E(E, care/ully
mo3e the (+RSE F+(S 72+B until the
specimen comes into 3iew.
;. To switch to the H#%H $+6ER +B8E(T#9E
*E2S, loo& at the microscope /rom the side.
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34 teps to%sing the Microscope
1 into place. #/ the
lens hits the slide, the slide and?or lens might
@rea&.
Turn the F#2E F+(S 72+B until the
specimen comes into /ocus.
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Specimen
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Answer Each Question
3. The lens closest to your eye when you use amicroscope is the 5555555555.
6. /hen using the microscope1 always beginwork with the 5555555555 objective rst.
7. /hich knob should you never use while onmedium or high power8 ,9plain why.
:. The lens closest to the slide is the 55555555555.
;. /hen using the coarse focus knob1 how
should you be view the slide8 ,9plain why.
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,nd of discussion