3. Prelab

Isolation of Chlorophyll and Carotenoids from Spinach Exp. 17

BOSTON UNIVERSITYDRESDEN SCIENCE PROGRAM

ORGANIC CHEMISTRY LABORATORY CAS CH 203, FALL 2014

Exp. 15 - Isolation of Chlorophyll and Carotenoids from Spinach

Exp. 15 - Isolation of Chlorophyll and Carotenoids from Spinach

Chloroplasts contain colored compounds (pigments) which fall into two categorieschlorophylls and carotenoids

Exp. 15 - Isolation of Chlorophyll and Carotenoids from Spinach

Chlorophyll b: -CHO group instead of shaded methyl groupPheophytin a: identical to chlorophyll a except that Mg2+ is replaced by two H+

Pheophytin b: identical to chlorophyll b except that Mg2+ is replaced by two H+

Exp. 15 - Isolation of Chlorophyll and Carotenoids from Spinach

Exp. 15 - Isolation of Chlorophyll and Carotenoids from Spinach

Exp. 15 - Isolation of Chlorophyll and Carotenoids from Spinach

-Carotene

-Carotene

-Carotene

Different position of the double bond

Xanthophylls are oxidized derivatives of carotenes. They contain hydroxyl groups and are more polar.

Exp. 15 - Isolation of Chlorophyll and Carotenoids from Spinach

Carotene is responsible for the orange colour of the carrots and many other fruits and vegetables.Xanthophylls are oxidized derivatives of carotenes. They contain hydroxyl groups and are more polar.

Exp. 15 - Isolation of Chlorophyll and Carotenoids from Spinach

Exp. 15 - Isolation of Chlorophyll and Carotenoids from Spinach

Exp. 15 - Isolation of Chlorophyll and Carotenoids from Spinach Technique 12 – Column Chromatography

Exp. 15 - Isolation of Chlorophyll and Carotenoids from Spinach Technique 12 – Column Chromatography

Exp. 15 - Isolation of Chlorophyll and Carotenoids from Spinach Technique 12 – Column Chromatography

Exp. 15 - Isolation of Chlorophyll and Carotenoids from Spinach Technique 12 – Column Chromatography

Exp. 15 - Isolation of Chlorophyll and Carotenoids from Spinach Technique 12 – Column Chromatography

Exp. 15 - Isolation of Chlorophyll and Carotenoids from Spinach Technique 12 – Column Chromatography

Exp. 15 - Isolation of Chlorophyll and Carotenoids from Spinach Technique 12 – Column Chromatography

Exp. 15 - Isolation of Chlorophyll and Carotenoids from Spinach Technique 12 – Column Chromatography

Exp. 15 - Isolation of Chlorophyll and Carotenoids from Spinach Technique 12 – Column Chromatography

Exp. 15 - Isolation of Chlorophyll and Carotenoids from Spinach Technique 12 – Column Chromatography

Exp. 15 - Isolation of Chlorophyll and Carotenoids from Spinach Technique 12 – Column Chromatography

Exp. 15 - Isolation of Chlorophyll and Carotenoids from Spinach Technique 12 – Column Chromatography

Exp. 15 - Isolation of Chlorophyll and Carotenoids from Spinach Technique 12 – Column Chromatography

Exp. 15 - Isolation of Chlorophyll and Carotenoids from Spinach Technique 14 – Thin-Layer Chromatography

Exp. 15 - Isolation of Chlorophyll and Carotenoids from Spinach Technique 14 – Thin-Layer Chromatography

Exp. 15 - Isolation of Chlorophyll and Carotenoids from Spinach Technique 14 – Thin-Layer Chromatography

Exp. 15 - Isolation of Chlorophyll and Carotenoids from Spinach Technique 14 – Thin-Layer Chromatography

Exp. 15 - Isolation of Chlorophyll and Carotenoids from Spinach Technique 14 – Thin-Layer Chromatography

Exp. 15 - Isolation of Chlorophyll and Carotenoids from Spinach Technique 14 – Thin-Layer Chromatography