25.4.2006 Distributor Meeting Landshut 2006

NEW PRODUCTS

Sera

Cell Culture Media - Classical

Special Media

Reagents

Growths Supplements

Transfection Reagents

Cell Preservation

25.4.2006 Distributor Meeting Landshut 2006

NEW PRODUCTS SERA

FBS – New Origins

EU approved: Argentina, Brasil

USA origin: North America

AUSTRALIEN origin: Australia

CANADIAN origin: Canada

USDA approved: Central America - Mexico

25.4.2006 Distributor Meeting Landshut 2006

NEW PRODUCTS SERA

FBS, PAA Clone

very low Endotoxin = under 1 EU / ml

various origins

25.4.2006 Distributor Meeting Landshut 2006

NEW PRODUCTS SERA

FBS Pre-Tested

FBS ES (Embryonic Stem Cells) tested

supports the growth of undifferentiated colonies of ES cells

recommended for most primary cells

for low unspecific stimulation

25.4.2006 Distributor Meeting Landshut 2006

NEW PRODUCTS SERA

FBS Pre-Tested

FBS Amnion Cell tested

cultivating of amnion and chorionic villi cells

cultivation of lymphocytes

pre and post –natal diagnostics

Karyotyping

FISH - analysis

25.4.2006 Distributor Meeting Landshut 2006

NEW PRODUCTS SERA

FBS Pharma Grade

= formally known as „FBS EMEA tested“

origins: USA and AUSTRALIA

standard and gamma irradiated

BVD antibody negative

Tested according to: Ph. Eur. 5.4 monograph 4/2006:2262

the EU regulation 2005/567 AT

CPMP/BWP 1793/02

CVMP/ 743/00

9CFRPH

25.4.2006 Distributor Meeting Landshut 2006

NEW PRODUCTS SERA

FBS Neo-Natal

collected from calves less than 12 hours old

prior to ingestions of colostrums

economical alternative to FBS

USDA approved

25.4.2006 Distributor Meeting Landshut 2006

NEW PRODUCTS CELL CULTURE MEDIA

DMEM

with Stable Glutamine

without Phenol Red

with D-Valine, without L-Valine, +/- L-Glutamine

without Calcium

without Glucose

thermostable, without L-Glutamine

25.4.2006 Distributor Meeting Landshut 2006

NEW PRODUCTS CELL CULTURE MEDIA

RPMI 1640

with Stable Glutamine

Thermostable without L-Glutamine

25.4.2006 Distributor Meeting Landshut 2006

NEW PRODUCTS SPECIAL MEDIA - Neuronal

Neuronal Base Medium

Serum free cell culture of neuronal cells

For reduction of glial cells

Optimal growth of embryonic neurons

For proliferation of neuronal stem cells

25.4.2006 Distributor Meeting Landshut 2006

NEW PRODUCTS SPECIAL MEDIA - Neuronal

Neuronal Base Medium AD

AD = adult neurons

Serum free

Long term cultivation of postnatal rat hippocampal

and other adult mammalian neurons

Long term cultivation of different adult neurons from the CNS and PNS

25.4.2006 Distributor Meeting Landshut 2006

NEW PRODUCTS Growths Supplements - Neuronal

B27 NeuroMix

G5 Supplement

N2 Supplement

Neuronal Stem Cell Supplement

25.4.2006 Distributor Meeting Landshut 2006

NEW PRODUCTS SPECIAL MEDIA – Stem Cell Media

CollagenStem Kit = Medium & Solution

Detection and quantification of hematopoietic progenitors

Formation and isolation of distinct colonies

Cytochemical or immuno-cytochemical staining

Serum free basal medium

Good optical clarity

Easy visualisation

25.4.2006 Distributor Meeting Landshut 2006

NEW PRODUCTS SPECIAL MEDIA – Stem Cell Media

MethoStem Medium

Growth and differentiation of hematopoietic stem cells

Contains methylcellulose

Differentiation and characterization of stem cell progenitors

For further cultivation and separation of single cells by colony selection

Growth of embryonic stem cells

25.4.2006 Distributor Meeting Landshut 2006

NEW PRODUCTS REAGENTS

Stable L-Glutamine (L-Alanyl-L-Glutamine) No toxic ammonium ions build up due to degratation processes

Incubating: 37°C

Storage: 15°C - 25°C

Direct substitute for L-glutamine = stable dipeptide form

For mammalian cell cultures

For suspension and adherent cell cultures

Essential media component

C

25.4.2006 Distributor Meeting Landshut 2006

NEW PRODUCTS REAGENTS

ALFAZYME contains a mixture of strong negative charged particles

+ mild acting enzymes

for sensible cell lines

more gentle

25.4.2006 Distributor Meeting Landshut 2006

NEW PRODUCTS TRANSFECTION REAGENTS

Nanofectin Kit (Nanofectin & Diluent)

Transfection reagent for mammalian cells

consists of a positively charged polymer and porous nanobeads

efficient binding of DNA

protects bounded DNA against degradation

high Transfection Rate

25.4.2006 Distributor Meeting Landshut 2006

NEW PRODUCTS CELL PRESERVATION

CoolStar

Optimized medium for hypothermic preservation (2°C to 8°C)

Preservation of different mammalian cells, tissues and organs

Gentle cold storage

Transportation of cells

25.4.2006 Distributor Meeting Landshut 2006

NEW PRODUCTS CELL PRESERVATION

CoolStar Kit = CoolStar &

CoolStar Rinse

flush solution

to rinse culture media and native fluids from mamma lian cells, tissues and

organs in combination with CoolStar

25.4.2006 Distributor Meeting Landshut 2006

NEW PRODUCTS CELL PRESERVATION

CryoMaxx

Optimized cryopreservation (liquid nitrogen)

minimized dehydration effects

also for serum containing media

for a wide variety of cell types

for cell banking

25.4.2006 Distributor Meeting Landshut 2006

25.4.2006 Distributor Meeting Landshut 2006

CYTOGENETICS

CYTOGENETICS IS….

the study of normal and abnormal CHROMOSOMES

25.4.2006 Distributor Meeting Landshut 2006

CYTOGENETICS PRENATAL

A variety of techniques to determine the health and condition of an unborn fetus

by analysis of:

Amnion Fluid (>14th week)Chorionic Villi Cells (>10th week)

25.4.2006 Distributor Meeting Landshut 2006

CYTOGENETICS PRENATAL

INDICATORS

maternal age – more than 35 years earlier abortion parental chromosomal aberration abnormalities at ultrasonography mutagene exposure

25.4.2006 Distributor Meeting Landshut 2006

CYTOGENETICS POSTNATAL

Diagnosis after birth and later…

by cultivation of T-Lymphocytes (3-4 days) out of 5ml whole blood

25.4.2006 Distributor Meeting Landshut 2006

CYTOGENETICS POSTNATAL

IndicatorsStrange phenotypeEnzymatic disorderLater: strange growth behaviour of childrenSterility problems

25.4.2006 Distributor Meeting Landshut 2006

CYTOGENETICS

Numerical Aberation

Trisomy 21 – Down Syndrome

25.4.2006 Distributor Meeting Landshut 2006

CYTOGENETICSA list of diseases based on numerical abarations

Adrenogenitales Syndrom (AGS) Alpha-1 Antitrypsin-Mangel Anenzephalie Angelman-Syndrom APC-Resistenz Autismus Bluterkrankheit Chorea Huntington Creutzfeldt-Jakob Disease Cystische Fibrose Dandy-Walker-Fehlbildung Down-Syndrom Dysbetalipoproteinämie Edwards Syndrom Eisenspeicherkrankheit Faktor-V-Leiden Fatal familial insomnia Fischschuppen-krankheit Fragiles-X-Syndrom Gaucher-Syndrom Gerstmann-Sträussler-S. Syndrom Hämochromatose Homocystinurie Huntington-Krankheit Hutchinson-Gilford-Progerie-Syndrom Hypercholesterinämie Hyperlipidämie Typ III Ichthyose

Hyperlipidämie Typ III Isolierte Lissenzephalie-Sequenz Katzenschrei-Syndrom Lesch-Nyhan-Syndrom Lissenzephalie-Sequenz, isolierte Martin-Bell-Syndrom Miller-Dieker-Syndrom Morbus Fabry Morbus Gaucher Morbus Osler Mukoviszidose Muskeldystrophie Neurofibromatose Noonan-Syndrom Osteoporose Pätau Syndrom Phenylketonurie (PKU) Prader-Willi-Syndrom Progerie Typ I Progerie Typ II Prothrombin-Mutation Recklinghausen-Krankheit Retinoblastom Salzverlustsyndrom Trisomie 13 Trisomie 18 Ullrich-Turner-Syndrom

25.4.2006 Distributor Meeting Landshut 2006

CYTOGENETICS

STRUCTUAL ABARATIONS

17p13.3 – Miller Dieker Syndrom

Loss of chromosomalMaterial = DELETION

25.4.2006 Distributor Meeting Landshut 2006

CYTOGENETICS PRENATAL

Quantum 3-21

Complete medium for prenatal diagnostics

No supplementation necessary

Selective cultivation of amniotic fluid cells and chorinonic villi cells

Ready to use medium with FBS, growth factors, hormones and

optimised base medium

For use in karyotyping

Tested in independent clinical laboratories

CE - certified

25.4.2006 Distributor Meeting Landshut 2006

CYTOGENETICS PRENATAL

Ready to use media

Rapid growth

No chromosomal breakages

Simple detection of chromosome aberrations

Quick results

Cell harvest after 7-10 days

For clear diagnostic findings

Unlimited availability

Quantum 3-21

25.4.2006 Distributor Meeting Landshut 2006

CYTOGENETICS PRENATAL

Who needs Quantum 3-21

Chromosomal Analysis

Institutes for Human Biology

State and Private Diagnostic Laboratories

Research in Human Genetics

Child Welfare Clinics

Clinical Research Labs

25.4.2006 Distributor Meeting Landshut 2006

CYTOGENETICS PRENATAL

Competition Quantum 3-21

AmnioMax, Invitrogen (USA, market leader)

Amnio Chrome,Cambrex (USA)

Chang Medium, Irvine (USA)

BioAMF1/2, Biological ind. (Israel)

25.4.2006 Distributor Meeting Landshut 2006

CYTOGENETICS POSTNATAL

Complete medium for postnatal diagnostics

Optimised media for T- Lymphocytes

No supplementation necessary

Media with pretested FBS

Pre-tested and optimal adjusted PHA

CE – certified product

Quality control in independent cytogenetic laboratories

Quantum PBL

25.4.2006 Distributor Meeting Landshut 2006

CYTOGENETICS PRENATAL

Who needs Quantum PBL

Institutes for Human Biology

Diagnostic laboratories

Clinical diagnostics

Research laboratories working with lymphocytes

Clinical research labs

25.4.2006 Distributor Meeting Landshut 2006

CYTOGENETICS PRENATAL

Competition Quantum PBL

KaryoMax, Invitrogen (USA, market leader)

LymphoChrome, Cambrex (USA)

Complete product line, Biological ind. (Israel)

Home made Media on base of Ham´s F12 + 20% FBS

25.4.2006 Distributor Meeting Landshut 2006

CYTOGENETICS

CE - Certification

CE certified product from a supplier

Nearly the same standard like cGMP

Declaration of conformity for CE marked products

Products must be produced in conformity to „Directive 98/79/EC“

Aim: constant quality (important for human diagnostics)traceability of the product must be

guaranteedAudit by legal authorisation is possible

25.4.2006 Distributor Meeting Landshut 2006

CYTOGENETICS MARKETING

Explaining features and benefits of Quantum media

Offering free samples

Offering the Cytogenetic brochure

Samples will processed by distributor

Quotes will be done from distributor