sp

P

H

ncnaa

(ntwgPfl

attscmnEwaTi

d

pl

rhdpcept

abpoc((clm

spco

i

Y

tcamteiv

ated1Eap

www.AJOG.org Clinical Obstetrics, Neonatology, Physiology-Endocrinology Poster Session I

Levels of HSP90 in term human placentas in relation to labor andmaternal consumption of pomegranate juice

223 Correlation between maternal and neonatalerum concentrations of long-chainolyunsaturated fatty acids (LCPUFA)

Michael Wolfe1, Lu-Te Chuang2, William Rayburn3,ei-Chi Wen2, Dorothy Vander Jagt1, Robert Glew1

1University of New Mexico, Albuquerque, NM, 2Yuanpei University,sinchu, 3University of New Mexico School of Medicine, Albuquerque, NM

OBJECTIVE: Recent studies have shown that the nutritional status ofonpregnant women in New Mexico with regard to LCPUFAs [ara-hidonic acid (AA), eicosapentaenoic acid (EPA) and docosahexae-oic acid (DHA)] is suboptimal relative to international norms. Ourim was to correlate plasma levels of LCPUFA between the mothernd neonate.

STUDY DESIGN: The fatty acid composition of the serum phospholipidsPL) of mother’s blood and umbilical cord blood of 52 maternal:eonatal pairs were compared. These samples came from consecu-

ively delivered, uncomplicated, term pregnancies. Maternal samplesere collected at admission, and umbilical cord blood samples wereathered at delivery. Plasma was extracted with chloroform methanol.hospholipids were separated by thin-layer chromatography, and

atty acid methyl esters were prepared and then separated by gas-iquid chromatography.

RESULTS: Although the mean percentages of AA (5.0%), EPA (0.3%)nd DHA (2.2%) in maternal serum PL were below those reported inhe literature for European and North American women at delivery,he percentages of AA (9.9%), EPA (0.6%) and DHA (3.5%) in corderum PL were much higher and within international norms. Theontent of DHA in the PL of the newborns correlated positively withaternal DHA status (r � �0.52, p � � 0.001), as did maternal and

ewborn AA (r � �0.49, p � 0.001). However, the percentages of AA,PA, and DHA in the serum PL of the newborns were not correlatedith birthweight, length, or head circumference. The highest percent-

ge of DHA in PL was found in the two Asian subjects (4.2 �/- 0.4%).he two Caucasian subjects and thirty-eight Hispanic subjects had

ntermediate DHA levels (2.17-2.31%), while the five American In- 0

Supplem

ian women had the lowest DHA percentage in their PL (1.4 �/-0.3%). There was a negative correlation between parity and the per-centage of DHA in maternal serum (r � -0.22, p � 0.04).CONCLUSIONS: Despite less than optimal serum LCPUFA levels of our

regnant women, their newborns managed to accrue normal plasmaevels of these fatty acids.

224 Obesity programs neonatal metabolic syndromeMina Desai1, Cristiane Guberman1, Michael G. Ross1

1LABioMed at Harbor-UCLA Med. Ctr., Torrance, CAOBJECTIVE: Greater than 65% of US adults are overweight or obeseepresenting an obesity epidemic. Offspring born to mothers with aigh body mass index or in pregnancies complicated by gestationaliabetes show increased obesity and diabetes risk in later life. As therevalence of obesity among pregnant women continues to rise, in-reasing number of children are exposed to an ’obese intrauterinenvironment’ during development. We thus investigated whether ex-osure to maternal obesity in prenatal and postnatal period programshe risk of obesity in the offspring.

STUDY DESIGN: Rats were fed a high fat diet (HF; 60% k/cal) from 3weeks of age, mated at 11 weeks, and maintained on a HF dietthroughout pregnancy and lactation. A control group was fed labora-tory chow (10% k/cal). At 1 day of age, litter size was standardized andblood collected from excess pups. All offspring were nursed by theirown dams. At 3 weeks of age, male offspring underwent DEXA scanand were fasted overnight for blood samples.RESULTS: Pregnant HF dams were significantly heavier than controlst conception (301�9 vs 233�8 g). At birth, HF newborns had similarody weights as the Controls (7.4�0.2 vs 7.3�0.1 g) though decreasedlasma leptin levels (2.1�0.5 vs 4.9�0.9 ng/ml). At 3 weeks of age, HFffspring exhibited accelerated growth, resulting in significantly in-reased body weight (175�4 vs 146�3 g) and percent body fat12.6�1.2 vs 6.4�1.0 %) with decreased percent lean body mass85.5�1.3 vs 91.6�1.0 %). Additionally, HF offspring exhibited in-reased fasting blood glucose (131�6 vs 96�7 mg/dl), and plasmaeptin (3.9�0.5 vs 1.3�0.2 ng/ml) and triglyceride (103�12 vs 68�8

g/dl) levels.CONCLUSIONS: Despite no differences in body weights at birth, off-pring of obese dams exhibit remarkably increased body weight, adi-osity and metabolic abnormalities. Thus, maternal obesity duringritical periods of development may increase the susceptibility of theffspring to metabolic syndrome early in life.

225 Placental global DNA methylationn a mouse model of fetal programming

Monica Longo1, Maged Costantine1, Phyllis Gamble1, Huaizhiin1, Nathan Drever1, Gary D.V. Hankins1, George R. Saade1

1The University of Texas Medical Branch, Galveston, TXOBJECTIVE: The placenta regulates nutrient transport and plays a cen-ral role in fetal growth. Disturbances in placental function duringritical periods of development set the stage for fetal programming ofdult diseases. DNA methylation of placental genes may be one of theechanisms by which placental function is altered and fetal adapta-

ions leading to developmental programming occur. Our goal was tovaluate the global DNA methylation status in a previously character-zed transgenic mouse model of utero-placental insufficiency and fetalascular programming.

STUDY DESIGN: Male and female homozygous NOS3 knockout (KO)nd wild-type mice (WT) were cross-bred to obtain KO and WT lit-ers, as well as genomically-similar heterozygous litters developing inither a normal (paternally-derived; KOP) or abnormal (maternally-erived; KOM) uterine environment. The dams were sacrificed at day8 of gestation, and the placentas were harvested for DNA isolation.LISA based colorimetric kit, was used to detect global DNA methyl-tion. Percent of hypermethylated DNA was determined and com-ared between groups using one-way ANOVA (significance: P �

.05).

ent to JANUARY 2011 American Journal of Obstetrics & Gynecology S97