22 11 93 2 7 25 - Sinawebqjaphd.sinaweb.net/article_541975_c4dd1ef99b49d2bfc98b... · 2020. 10....

12
25 7 2 93 11 22 (Saccharomyces cerevisia) - (Oncorhynchus mykiss) 1 2 2 1 - [email protected] 2 - . 16 / 1 / 93 27 / 2 / 93 ) Saccharomyces cerevisia - . 120 12 / 0 ± 61 / 3 8 1 5 / 1 2 3 2 ) 05 / 0 P< .( ) 05 / 0 P> .( IgM Ig 2 05 / 0 P< .( 2 . ) 35 .( 3 .( 43 .( . 5 4 .( -

Transcript of 22 11 93 2 7 25 - Sinawebqjaphd.sinaweb.net/article_541975_c4dd1ef99b49d2bfc98b... · 2020. 10....

Page 1: 22 11 93 2 7 25 - Sinawebqjaphd.sinaweb.net/article_541975_c4dd1ef99b49d2bfc98b... · 2020. 10. 5. · 13 93 2 7 25 1 120 10 3/61±0/12 100 12 3 4 100 2 1/5 1-(Binder, Germany 24

2572931122

(Saccharomyces cerevisia)

-

(Oncorhynchus mykiss) 122

1- [email protected]

2- .16/1/93 27/2/93

) Saccharomyces cerevisia -.

12012/0±61/3 8 15/123

2

)05/0P< .( )05/0P>.(

IgM Ig 2 05/0P< .(

2

.

)35.(

3.(

43 .(

.

54.( -

Page 2: 22 11 93 2 7 25 - Sinawebqjaphd.sinaweb.net/article_541975_c4dd1ef99b49d2bfc98b... · 2020. 10. 5. · 13 93 2 7 25 1 120 10 3/61±0/12 100 12 3 4 100 2 1/5 1-(Binder, Germany 24

12

-

20 .(

Saccharomyces cerevisia

-Glucan)31 MOS ()19 .(

16( .

33 .(

)Cyprinus carpio( 38

3)13 .(1 23%

8

)05/0P>()1(.S.cerevisiae (

Sparus aurata

IgM

IgM

)15(05/01 5/1 %Saccharomyces

cerevisiae

8

5/1-

)11(. 2///0

8/0 1)MOS(

34895

13)MOS

3-

10.(Labeo rohita 8055/710

39.(

-

8

1391 -

Page 3: 22 11 93 2 7 25 - Sinawebqjaphd.sinaweb.net/article_541975_c4dd1ef99b49d2bfc98b... · 2020. 10. 5. · 13 93 2 7 25 1 120 10 3/61±0/12 100 12 3 4 100 2 1/5 1-(Binder, Germany 24

2572 93 13

1 120

12/0±61/310 100

4312 100

15/12

-

(Binder, Germany

30 24

-

15 -

01/0Shinko

Radwag WTB

-

5 -33)81420

2

8

5 %3

3096(.

WTW330i

Weilheim 01/0300HM

.

243

cc 2.

40.(

cc15/0

5/0)39( .

Awareness, USAStat Fax-

2100

(mg/ml) 4022.(

Page 4: 22 11 93 2 7 25 - Sinawebqjaphd.sinaweb.net/article_541975_c4dd1ef99b49d2bfc98b... · 2020. 10. 5. · 13 93 2 7 25 1 120 10 3/61±0/12 100 12 3 4 100 2 1/5 1-(Binder, Germany 24

14

MNephelometry

IgM

IgM

Minineph)(Binding site U

nm340 400 800

IgM 4523 7.(

Elisa Reader )Awareness, USAStat Fax-

2100

mg/ ml-1)27(

Shapiro-Wilk

5% SPSS17

2003 Excel

11/3± 75/17 pH 41/0 ± 35/72/1 ±2/7

52/0±5/282

05/0P<(1(.

3 )74/655±9500 mm3

3 )88/0 ± 33/39 2)20 /1 ±

33/30 3 2

1.

05/0P>(1(. 284/3±

67/50 57/0± 4 33/0±33/1

2

05/0<P(.

3)72/39849±800000 mm3 (2 )99/18036±717000mm3 ( 1 2

)15/0±6 gr/dl(3 )15/0± 5/6 gr/dl

1 )26/0±77/4 )2/0±4/4 2

3 (MCV)

Page 5: 22 11 93 2 7 25 - Sinawebqjaphd.sinaweb.net/article_541975_c4dd1ef99b49d2bfc98b... · 2020. 10. 5. · 13 93 2 7 25 1 120 10 3/61±0/12 100 12 3 4 100 2 1/5 1-(Binder, Germany 24

2572 93 15

(MCH) (MCHC)

05/0P>(2(MCV) 5/104/5 ±33/418

(MCH)5/1) %33/0±33/83(MCHC)1) %43 /0±36/20

1 -

12 3

a 250 ±5250 a16 /208± 5800 b37/240 ±33/7533c74/655±9500a1±20 a57/0 ±21b20/1 ±33/30c88/0 ±33/39

1 ± 78 28/3±33/7315/1 ±6784/3 ± 67/50

5/0±5/1 33/0 ±66/133/0± 33/2 57/0 ± 4

33/0±33/0 33/0 ±66/033/0± 33/033/0 ±33/1 5

2 -

1 3 a26/45706 ± 541250a18/10837±67/586666 ab 99/18036±717000b72/39849± 800000

gr.dcl( a2/0±4/4a26/0±77/4 b15/0±6 b15/0±5/6 a5/0± 50/23a 88/0± 33/23 b 58/0±30b 33/1±67/32

5/1 ±5/41426/10±397 04/5±33/41839/10 ± 33/408

)pp(1±82 84/3±66/80 33/0 ±33/83 08/2 ± 81

)gr.dcl(1 ± 19 43/0±36/2020/0± 96/1936/0 ±9/19 5

1(2%82/15± 106 U/ml/min

1 ± 13 U/ml/min

5/1205/0P< .(

M)IgM

2IgM206/19±67/ 116

5/24 3

Page 6: 22 11 93 2 7 25 - Sinawebqjaphd.sinaweb.net/article_541975_c4dd1ef99b49d2bfc98b... · 2020. 10. 5. · 13 93 2 7 25 1 120 10 3/61±0/12 100 12 3 4 100 2 1/5 1-(Binder, Germany 24

16

Ig

3Ig2

85/1±33/22 16

)05/0P<.(

1- 5(

2- IgM

5(

3- Ig

5(

a

b

c

a0

50

100

150

1 2 3

a

ab

b

a

0

50

100

150

1 2 3

ab

b b

a

05

1015202530

1 2 3

Page 7: 22 11 93 2 7 25 - Sinawebqjaphd.sinaweb.net/article_541975_c4dd1ef99b49d2bfc98b... · 2020. 10. 5. · 13 93 2 7 25 1 120 10 3/61±0/12 100 12 3 4 100 2 1/5 1-(Binder, Germany 24

2572 93 17

14.(

)2

)36

)05/0P>(

12

2-

-

.

123 )2

-

252

1

Co2

)1295

44 .(

38

-

)38(MCVMCHMCHC

)05/0P>((MCV)

(MCH)

Page 8: 22 11 93 2 7 25 - Sinawebqjaphd.sinaweb.net/article_541975_c4dd1ef99b49d2bfc98b... · 2020. 10. 5. · 13 93 2 7 25 1 120 10 3/61±0/12 100 12 3 4 100 2 1/5 1-(Binder, Germany 24

18

)38.(

)Ig( M)IgM(

)05/0P<.(

-

30Ig-

-

41

25 S. cerevisiae

5 5/7 10 Labeo rohita

)39(. 100250500

Labeo rohita 31

28.( MOS )32 .(

Edwardsiella ictaluri

)4429(.

)37.(

)31(

83)2

2

3216.(

S.cerevisiae

100 -80021

05/0P>()1817.(

IgIgM

3

Page 9: 22 11 93 2 7 25 - Sinawebqjaphd.sinaweb.net/article_541975_c4dd1ef99b49d2bfc98b... · 2020. 10. 5. · 13 93 2 7 25 1 120 10 3/61±0/12 100 12 3 4 100 2 1/5 1-(Binder, Germany 24

2572 93 19

)05/0P<()13.( 8

Ig IgM

11 41 510gr/kg

(Sparus aurata)100200IgM

14( .

IgM )26.(

0 1 2 60

MCHMCVMCHC -

21.(246

MOS(D. labrax)

608

MOS40 .(

242 %

-

Ig IgM

)

( )

(

)422 .(

Page 10: 22 11 93 2 7 25 - Sinawebqjaphd.sinaweb.net/article_541975_c4dd1ef99b49d2bfc98b... · 2020. 10. 5. · 13 93 2 7 25 1 120 10 3/61±0/12 100 12 3 4 100 2 1/5 1-(Binder, Germany 24

20

3

2 %

1-. 1386 .

(Oncorhynchus mykiss) .19.

2- . . .1389 . . 1( .9

.3-1381.

10

17.4-. .1386 .

104.

5- .1387 .

101

6- ... . .1382 .

. . 37 48 .

7-1382-

. . 268 .

8-1387..264

9 - . . . .. .1384 .

-

.2 4149.

10- .1389 . Immunoster Immunowall

. 126

.11-1390

Saccharomyces cerevisiae

(Acipenser nudiventris) . .. 100

12-. . . .. .1389 .

.194 .

13-1390

Page 11: 22 11 93 2 7 25 - Sinawebqjaphd.sinaweb.net/article_541975_c4dd1ef99b49d2bfc98b... · 2020. 10. 5. · 13 93 2 7 25 1 120 10 3/61±0/12 100 12 3 4 100 2 1/5 1-(Binder, Germany 24

2572 93 21

)Cyprinus carpio..90

14. Bahmani, M., Kazemi, R., Donskaya, P. (2001). Acomparative study of in young reared sturgeons (Acipenser persicus and Huso huso). Fish Physiology and Biochemistry, 24; 135-140.15. Cuesta, A., Meseguer, J., Esteban, M.A. (2004). Total serum immunologlobulin M levels are affected by immunomodulators in sea bream (Sparus auratea). Veterinary Immunology and Immunopathology, 101; 203-210.16. Dalmo, A.R., Bogwald, J. (2008 -glucans as conductors of immune symphonies. Fish and Sellfish Immunology, 25; 384-396. 17. David, J.A., Jenkiss, Cyrill, W.C., Vladimir, V. (1999). Inuline oligofructose and intestinal function. J. Nutr, 129; 1431-1433. 18. EL-Boshy, M.E., El-Ashramb, A.M., Abdel hamid, F.M., Gadalla, H.A. (2010). Immunomodulatory effect of dietary Saccharomyces cerevisiae -Glucan and laminaran in mercuric chloride treated Nile tilapia (Oreochromis niloticus) and experimentally infected with Aeromonas hydrophila. Fish & Sellfish Immunology, 28; 802-808.19. Gang, L., Huang, G.L. (2008). Extraction of two active polysaccharides from the Yeast cell wall. Z. Naturforsch, 63c; 919-921. 20. Gibson, G.R. (1998). Dietary modulation of the human gut microflora using prebiotics. British Journal of Nutrition, 80(2); S209-S212. 21. Hoseinifar, S.H., Mirvaghefi, A.R., Mojazi Amiri, B., Khoshbavar, H.A., Pooramini, M., Bastami, D. (2011). The probiotic effect of dietary inactive yeast Saccharomyces cerevisiae var ellipsoideus on growth factors, survival, body composition and intestinal microbiota of Beluga juvenile (Huso huso). K-Iranian Scientific Fisheries Journal, 19( 4); 55-66. 22. Huang, S.S.O., Lutes,P.B., Storebakken, T. (1989). Growth and feed efficiency of white sturgeon (Acipenser transmontanus) sub0 yearling at different feeding f\rates. Aquacultuer, 80; 147-153.23. Khoshbavar- Rostami, H.A., Soltani, M. Kazemi, B., Hassan, M.D. (2003). Immune responses of great sturgeon (Huso huso) to Aeromonas hydrophial bacterin. Journal of FishBiology, 70; 931-938. 24. Linehan, S.A., Martinez-Pomares, L., Gordon, S. (2000). Macrophage lectins in host defence. Microbes and Infection, 2; 279-288.25. Luskova, V. (1998). Factors affecting hematological indices in free-living fish populations. Acta Vet, 67; 249-255.

26. Magnadottir, B., Jonsdottir, H., helgason, S., Bjornsson, B., T.O. Jorgensen, T.O., pilstrom, L. (1999). Humoral immune parameters of Atlantic cod (Gadus morhue). Vomparative Biochemistry and physiology, 122B; 173-188. 27. Malin, M., Suomalainen, H., Saxelin, M., Isolauri. E. (1996). Promotion of IgA immune response in patients with Crohns disease by oral bacteriotherapy with lactobacillus GC. Annual nutrition and metabolism, 40; 137-45.28. Merrifield, D.L., Bradley, G., Baker, R.T.M., Dimitroglou, A., Davies, S.J. (2009). Probiotic applications for Rainbow trout (Oncorhynchus mykiss Walbaum) I. Effects on growth performance, feed utilization, intestinal microbiota and related health criteria. Aqua. Nutr., Doi, 10.1111/j.1365-2095. 2009.00689.x.Accepted. 29. Misra, CK., Das, BK., Mukherjee, SC., Pattnaik, P. (2006). Effect of long term

-Glucan on immunity. Growth and survival of Labeo rohita fingerling. Aquacultuer, 255; 82-84.30. Mohseni, M., Pourkazemi, M., Bahmani, M., Falahatkar, B., Pourali, H.R., Salehpour, M. (2006). Effects of feeing rate and frequency on growth performance of yearling great sturgeon, Huso huso. Journal of Applied Ichthyology, 22;278-282. 31. Peter, H., Sneath, A. (1986). Bergeys manual of systematic. Bacteriology, 2; 1104-1154. 32. Ringo, E., Olsen, R.E., Gifstad, T.O., Dalmo, R.A., Amlund, H., Hemre, G.I., Bake, A.M. (2010). Prebiotics in aquaculture Nutrition, 16; 117-136.33. Roberts, R.J., Shepherd. C.J. (1997). Handbook of trout and Salmon diseases. Fishing News Books.34. Sado, R.Y., Almeida Bicudo, A.J.D., Cyriono, J.E.P. (2008). Feeding dietary mannan oligosaccharides to juvenile tilapia, Oreochromis niloticus, has no effect hematological parameters and showed decreased feed consumption. Journal of World Aquaculture Society, 39(60); 821-826. 35. Sedwick, S.D. (1990). Trout farming handbook, Fifth edition, Fishing News Book. 36. Shalaby, A.M., Khattab, Y.A., Abdel Rahman, A.M. (2006). Effects of Garlic (Allium sativum) and chloramphenicol on growth performance, physiological parameters and survival of Nile tilapia (Oreochromis mosambicus). J. Venom. Anim. Toxins incl. Trop. Dis, 12; 172-201.37. Staykov, Y., Spring, P., Denev, S., Sweetman, J. (2007). Effect of a manna oligosaccharide on growth performance and immunestatus of Rainbow trout (Oncorhynchus mykiss). Aquaculture. Int., 15; 153-161. 38. Tangestani, R., Alizadeh Doughikollaee, E., Ebrahimi, E., Zare, P. (2011). Effects of Garlic essential oils an Immunostimulant on

Page 12: 22 11 93 2 7 25 - Sinawebqjaphd.sinaweb.net/article_541975_c4dd1ef99b49d2bfc98b... · 2020. 10. 5. · 13 93 2 7 25 1 120 10 3/61±0/12 100 12 3 4 100 2 1/5 1-(Binder, Germany 24

22

Hematological indices of juvenile beluga (Huso huso). Journal of Veterinary Research, 66(3); 209- 216. 39. Tewary. A., Patra, B.C. (2011). Oral administration of baker,s yeast (Saccharomyces cerevisiae) acts as a growth prometer and immunodulator in Labeo rohita (Ham). J. Aqua. Res. Development, 2; 109.7 p.doi:10.4172/2155-9546.1000109.40. Torrecillas, S., Makol, A., Caballero, M.J., Montero, D., Gines, R., Sweetman, J., Izquierdo, M.S. (2010). Improved feed utilization, intestinal micus production and Immune parameters in sea bass (Dicentrachus labrax) fed mannan oligosaccharides (MOS). Aquaculture Nutrition, (In press). 41. Tukmechi, A., Rahmati, H.R., Manaffar, R., Sheikhzadeh, N. (2011). Dietary administration of beta-mercapto-ethanol treated Saccharomyces cerevisiae enhanced the growth, innate immune response and disease. Fish & Shellfish Immunology, 30; 923- 928.

42. Verdegem, M.C.J., Hilbrands, A.D., Boon, J.H. (1997). Influence of salinity and dietary composition on blood parameter values of hybrid red tilapia (Oreochromis niloticus) & (Oreochromis mosambicus). Aquaculture. Res, 28; 453-459.43. Vulevic, J., Rastall, R.A., Gibson, G.R. (2004). Developing aquantitative approach for determining the in Vitro prebiotic potential of dietary oligosaccharides. FENS Microbiology letters, 236; 153-159. 44. Welker, T., Lim, C., Yildirim- Aksoy, M., Sheby, R., Klesius, P.G. (2007). Immune response and resistance to stress and Edwardsiella ictalurichallenge in channel catfish, Ictalutus punctatus, fed diets containing commercial whole - cell yeast or subcomponets. Journal of the World Aquaculture Society, 38(1); 24-35.45. Zilva , JF., Pannall, PR. (1984). Clinical chemistry in diagnosis and treatment. Publ. Lioyd-Luke (medical Books) Ltd. London PP348-35.

.