2100 expert DNA 1000 DE72901621 2019-09-20 12-15-48 ... - DTU

Instrument Name: DE72901621 Firmware:Serial#:Assay Information:

C.01.069DE72901621

Instrument Information:

Assay Origin Path: C:\Program Files\Agilent\2100 bioanalyzer\2100expert\assays\dsDNA\DNA 1000 Series II.xsy

Assay Class:Version:Assay Comments:

DNA 10002.3DNA Analysis 25 -1000 bp © Copyright 2003-2009 Agilent Technologies, Inc.

Chip Information:

Chip Comments:

Type: G2939A

Chip Lot #:Reagent Kit Lot #:

Kursus 2019 library 1 Kursus 2019 library 2 Kursus 2019 library 3




2100 Expert (B.02.08.SI648) © Copyright 2003 - 2009 Agilent Technologies, Inc. Printed: 9/20/2019 2:04:21 PM

2100 expert_DNA 1000_DE72901621_2019-09-20_12-15-48.xad Page of1 12

Created:Modified:

9/20/2019 12:15:47 PM9/20/2019 12:57:39 PMData Path:

DNA 1000C:\...-20\2100 expert_DNA 1000_DE72901621_2019-09-20_12-15-48.xad

Assay Class:

Electrophoresis File Run Summary

Sample Name Sample Comment Rest. Digest Status Observation Result Label ResultColor

Kursus 2019 library 1Kursus 2019 library 2Kursus 2019 library 3Kursus 2019 library 4Kursus 2019 library 5Kursus 2019 library 6Kursus 2019 library 7Kursus 2019 library 8Kursus 2019 library 9Kursus 2019 library 10Kursus 2019 library 11Kursus 2019 library 12Ladder

Chip Lot # Reagent Kit Lot #

Chip Comments :



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Assay Class:

Electrophoresis File Run Summary (Chip Summary)

General Analysis SettingsNumber of Available Sample and Ladder Wells (Max.) : 13Minimum Visible Range [s] : 30Maximum Visible Range [s] : 129Start Analysis Time Range [s] : 30End Analysis Time Range [s] : 128.95Ladder Concentration [ng/µl] : 44Uses Standard Area for Ladder FragmentsLower Marker Concentration [ng/µl] : 4.2Upper Marker Concentration [ng/µl] : 2.1Used Upper Marker for QuantitationStandard Curve Fit is Point to PointShow Data Aligned to Lower and Upper Marker

Integrator SettingsIntegration Start Time [s] : 30Integration End Time [s] : 128.95Slope Threshold : 0.5Height Threshold [FU] : 20Area Threshold : 0.1Width Threshold [s] : 0.5Baseline Plateau [s] : 0.5

Filter SettingsFilter Width [s] : 0.5Polynomial Order : 4

Ladder Ladder Peak Size Area1 15 252 25 263 50 344 100 415 150 456 200 527 300 638 400 769 500 8310 700 8811 850 8612 1000 9013 1500 52



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Assay Class:

Electrophoresis Assay Details

Peak table for LadderPeak Size [bp] Conc. [ng/µl] Molarity [nmol/l] Observations Peak Height1 15 4.20 424.2 Lower Marker 115.52 22 0.00 0.0 5.33 25 4.00 242.4 Ladder Peak 89.54 50 4.00 121.2 Ladder Peak 95.75 100 4.00 60.6 Ladder Peak 115.26 150 4.00 40.4 Ladder Peak 201.67 200 4.00 30.3 Ladder Peak 214.28 300 4.00 20.2 Ladder Peak 236.99 400 4.00 15.2 Ladder Peak 231.710 500 4.00 12.1 Ladder Peak 237.211 700 4.00 8.7 Ladder Peak 224.812 850 4.00 7.1 Ladder Peak 225.513 1,000 4.00 6.1 Ladder Peak 215.414 1,500 2.10 2.1 Upper Marker 148.7

Ladder



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Assay Class:

Electropherogram Summary

Peak table for sample 1 : Kursus 2019 library 1Peak Size [bp] Conc. [ng/µl] Molarity [nmol/l] Observations Peak Height1 15 4.20 424.2 Lower Marker 131.02 345 0.92 4.0 21.53 358 0.69 2.9 24.14 378 0.95 3.8 26.85 387 0.37 1.5 28.16 392 0.47 1.8 28.17 402 0.36 1.4 27.88 413 0.81 3.0 28.69 433 1.02 3.6 26.610 464 0.69 2.2 22.311 1,500 2.10 2.1 Upper Marker 148.8

Kursus 2019 library 1

Overall Results for sample 1 : Kursus 2019 library 1Number of peaks found: 9



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Assay Class:

Electropherogram Summary Continued ...

Peak table for sample 2 : Kursus 2019 library 2Peak Size [bp] Conc. [ng/µl] Molarity [nmol/l] Observations Peak Height1 15 4.20 424.2 Lower Marker 133.12 341 3.48 15.4 29.93 357 0.80 3.4 29.64 369 1.50 6.2 28.05 397 0.27 1.0 21.06 1,500 2.10 2.1 Upper Marker 146.6





Created:Modified:

9/20/2019 12:15:47 PM9/20/2019 12:57:39 PMData Path:


Assay Class:


Peak table for sample 3 : Kursus 2019 library 3Peak Size [bp] Conc. [ng/µl] Molarity [nmol/l] Observations Peak Height1 15 4.20 424.2 Lower Marker 136.22 371 1.94 7.9 24.03 390 1.10 4.3 30.14 401 0.78 3.0 33.75 415 0.41 1.5 35.06 427 0.70 2.5 37.67 442 0.43 1.5 37.58 450 0.48 1.6 39.29 457 0.49 1.6 38.710 477 0.47 1.5 39.111 490 0.63 1.9 38.212 499 2.07 6.3 37.313 565 1.62 4.3 28.614 1,500 2.10 2.1 Upper Marker 146.0





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Assay Class:


Peak table for sample 4 : Kursus 2019 library 4Peak Size [bp] Conc. [ng/µl] Molarity [nmol/l] Observations Peak Height1 15 4.20 424.2 Lower Marker 136.02 356 4.92 20.9 45.73 363 0.69 2.9 47.14 374 1.29 5.2 49.15 383 1.65 6.5 51.86 398 1.15 4.4 50.97 414 5.44 19.9 48.58 1,500 2.10 2.1 Upper Marker 138.8





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Assay Class:


Peak table for sample 5 : Kursus 2019 library 5Peak Size [bp] Conc. [ng/µl] Molarity [nmol/l] Observations Peak Height1 15 4.20 424.2 Lower Marker 140.42 446 5.69 19.3 53.23 454 0.70 2.3 55.44 471 0.66 2.1 58.15 491 0.75 2.3 60.56 504 1.32 4.0 61.87 527 1.05 3.0 63.48 541 0.76 2.1 62.59 566 1.17 3.1 62.510 584 1.42 3.7 62.711 612 2.00 4.9 61.112 646 5.75 13.5 57.113 1,500 2.10 2.1 Upper Marker 145.8





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Assay Class:


Peak table for sample 6 : Kursus 2019 library 6Peak Size [bp] Conc. [ng/µl] Molarity [nmol/l] Observations Peak Height1 15 4.20 424.2 Lower Marker 140.42 1,500 2.10 2.1 Upper Marker 140.2





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Assay Class:




Created:Modified:

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Assay Class:

Gel Image

Description Number Source Category Sub Category Time Time Zone User HostRun ended onport 1 (Numberof wellsacquired: 13)

Instrument Run 9/20/201912:57:35 PM

(GMT +02:00)RomanceStandard Time

Lab W-LAB02

Run started onport 1 (File:C:\ProgramFiles\Agilent\2100bioanalyzer\2100expert\Data\2019-09-20\2100expert_DNA1000_DE72901621_2019-09-20_12-15-48.xad)



Lab W-LAB02

ProductNumber :G2939A



Lab W-LAB02

Name : Instrument Run 9/20/201912:15:54 PM


Lab W-LAB02

Vendor :AgilentTechnologies



Lab W-LAB02

Serial# :DE72901621



Lab W-LAB02

Firmware :C.01.069



Lab W-LAB02

Cartridge :Electrode



Lab W-LAB02



Created:Modified:

9/20/2019 12:15:47 PM9/20/2019 12:57:39 PMData Path:


Assay Class:

Run Logbook

2100 expert DNA 1000 DE72901621 2019-09-20 12-15-48 ... - DTU

Documents

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