1

OT3bIBoQuqraamuoro olrroHenra A. 6.u. L'lnanona B.H.

Ha rl{cceprallurc 3ro6una lllr.su EereHres[qa <Pannue crpeccopHbre orBerbr pacrenufi parrca:naIIoBbIIIIeHHbIe ypOBHI,I MeAv LI qI,IHKa B CpeAe)),

rlpeAcraBneHHylo Ha col4cKaHue yrenofi creneHlr KaHAr4Aara 6raororu.recKr{x HayKno crrequanbHocrlr 03. 0 I . 05 - Srasuonorrn x u 6uoxutvtut paclre:ouit

,{nccepraqufl, Haartmaerct c KoporKoro <Bne4enux)), orrr4cbrBarorqero npo6JreMy, perxeHr4rororopofi AonxHo rloMorlb rlpe.4llpl,IHtroe pIccJIeAoBaHI4e, a r.rMeHHo - upo6lerr,ry Bnvr.fl]F'krr MeAr4 r4III4HKa Ha )KIa3HeAe{TeJIrHocm pacrenraft npr{ yBenr,rqeH}rr4 KoHueuTparlr4rr STux Mertu'rJroB B cpeAe.llpo6rervra ocraerct aKTyilrlbHofi, necruorp-a Ha rlpeArrp[Hrrbre pauee MHoroqlrcreHHbreLIccJIeAoBaHI,Is. OqesIaAHo, rrro .reNa 6oree rroJrHbrM 6yaer 3HaHrle MexaHH3MoB BIrprfl:rrvrr MeAr,r Hur.rHKa ua uera6orr,r3M KJreroK pacreHrafi, [pe)KAe Bcero, KJreroK KopHr, Koropbre rrepBbre Bcryrraror B6opr6y c MeraJIJIulMIr [pI4 cyruecrBeHHoM [oBbrrleHr{r4 coAep}Kanprs, nocJreAHr{x B cpeAe, revr 6onsurneBo3MoxHocrl{ orKpblBalorct Ang 3aIqLITu pacreHufi. Irlrvreugo BbIrIcHeHIrIIo eule ocraBrxrrxcflorKpbITbIMLI BorlpocoB Aeficreu-f, Me,u4 I4 IIIIIHKa Ha pacreHprq u rocBsrqena pa6ora 3rc6usa|4.F,.

B xosqe 3Tofo pa3Aena $opuynnpyerct Ilenb Al4cceprarluoHHofi pa6omr u nepeq[cnrrorctKoHKperHbIe 3ap.aqkr, peIrraBlrrlrect B npoqecce [poBenenux pa6orrr. Xors rr nprzHrro cefi.rac

$opuylzpoBarb 3aAauu pa6ol:'l B KoHIIe Bne4enux, JrorwrHee gro 6rrro 611 4elarr B KoHrIe O6sopanzreparypbl, B KaKoBoM cnfrae onu 6rr JIorI4lIHo cJleAoBanr{ 3a aHanr,r3oM HeperxeHHbrx Ao cr{x nopBonpocoB.

<O6:op nl4Teparypbl), 3aHllMaroquft Bcero rperbro rracrb Ar4cceprarlr4r{, coAepxlrr BcroocHoBH)'Io un(fopuaqraro, neo6xoAl4Mylo Anq [oHI4MaHufl cnryawrrd, cJroxr4nnefics B HayKe nplrI{ccJleAoBaHrara o6ogna.rennofi npo6neurr. Bce par4enr'r o6:opa rloKa3brBaror 3HaHr4e ocHosHoftnlrreparypbl o coAepxauvrv Me4kr r{ rIrrHKa B xr,rBbrx KJrerKax, o6 ux (poprr,rax B KJrerKe, MexaHr.r3MaxnoBpe)KAeHI,It Knerorlnbx crpyKTyp r.r orAeJlbHbrx 6r.ronaoneryr npr{ yBenr{rreHr4a coAep)KaHr,rtcno6oanrrx u cra6oces3aHHbrx r4oHoB grr,rx Mer€IJIJToB B KJreTKe.

Baxtras qacrb o6sopa nl4Teparypbl - orII{caH}Ie crparerr4r{ r{ MexaHr{3MoB 3arqr,rrbr pacrennfior roKclaqecKoro 4eficrnux MeAr{. B groN,r pa3Aene 3ro6un IrI.E. npe4craBJrrer lrMerorluecs Bnl{Teparype AaHHbIe o MexaHrl3Max 3arqrrrbr, Korophre B 3Haqr,rreJrtuofi creneHr4 non)qeHbr AntKJIeroK )Io,IBorHbD( I4 uenoBeKa. Xors rrMerorcf, pa6orrr, B Koropbrx r,r3yqanocb Aeficreae MeAr.r rrrIr4HKa Ha KJrerKpr pac:renrzft, Ho aBTop B 3aKJrroqeHrara O6sopa nrrreparypbr coBeprxeHHo crrpaBeAJrr4Borrr4rrrer <Ha cerornsmur,rft AeHb ocrarorcr orKpbrrbrMr,r Borrpocbr o xr4Mr4.recKofi flpupoAe ocHoBHbrxBHyrpI,IKneroqHhIX nI4raHAoB I{oHoB MeAr4 r4IIIIHKa B pacrlrrerrbHbrx KJrerKax npu us6rrme MeraJrnoB BcpeAe, o naxuefinrllx cr,rcreMaxvtx AeroKcKarlupr, a raKxe o KoHKperHbD( MexaHr{3Max rroBpexAeHr,rtKJrerorrHbrx crpyKTyp npra 4eficrBr4r,r grr4x MeraJrJroB.) CneAenus Anr perrreHvrs,3Tr4x BorrpocoB r4rrocTapancr [onrrr4Tb aBTop.

B qerou, o6:op cBuAereJrbcrByer o6 spyarauult 3ro6usaLl.E., rroHrdMaHr.ru prM rrprdpoAErprccneAyeMbrx rBneHl{fi, xopoureM 3HaHuI,I nl4Teparypbr tto l43yrlaeMbrM BonpocaM, a raKxe qcHoM

rrpeAcTaBneH'uu cyTH He peureHHbrx Ao cr.rx [op 3aAaq.fraea 2 yuccepr:a:qr,ru <Marepuur:;ru MeroAbr r4ccJreAoBaHr.rr)) rro3Boffer npeAcraBr,{Tb xoA

rrpoBe.4eHr.rs oKcrepraMenramnofi pa6oru. 3ra rlana [por.r3BoAr.rr oqeHb 6naronpuxruoeBrrer{arJreHrre. ,{eranrHo olucaHbr ycnoBr4r BblpalqrtBanl,lfl.pacrenufi ra npone4eHr,rs oflhrroB, qro

coBeprneuHo neo6xoAr.rMo B pa6orax uo Susuoroilau pacrenufi. flo4po6noe orrracaHr4eSKcnepr4MeHTaJIbHbD( MeroAoB, - (pusuororualecKllx, 6raoxutrruqecKlrx, MoneKynrpno-6zororuqecK[xHe ocraBJrrer coMHeHrrfi, uro 3no6ran I4.E. ruarenbuo [poBoAr{n oKcnepraMeHTaJrbHyro pa6ory.

Xopouro r.r3noxeHbr lr MeroAhr r{ccJreAoBanuft, rpeboBaBlrr{e oKpacKr.r o6pasqon lr anaJrn3ape3ynbraroB c r,rcnoJrb3oBaHueM Mr{KpocKorra. flpe4craureH H cnoco6 crarrrcrr4qecrofi o6pa6orxnpe3ynbraron. B qerou, 3Ta qacrb Ar.rccepralllrll cBl4AerenbcrByer o 3HaHr{r4 aBTopoM r4crroJrb3oBaHHhlxMeTOAOB I4 O TOM, qTO SKCnepIzMeHTbI IpOBOAI4JII'ICb LIM JII'IqHO.

2

B fnaee 3 <Pesymrarbl) ilpLIBeAeHbr AaHHbIe gKc[epr4MeHToB, Koropbre 6stnu [poBeAeHbr An.aperrreuur rrocraBJreHHbrx B pa6ore 3anarr.

Baxnrnn sra[oM pa6oru 6ruro I,BMepeHI,Ie o6uero coAepxanvr Me.qrr B TKaH.rrx Jrr4crbeB r.rropueft u r.I3MeHeHI4e ee coAepxaHvs rrprl yBeJILIqeHiltI coAep)Kanns. MerarrJra B cpeAe. Pesyltrarrrorllx rl3MepeHrafi snorne JIofI{rrHbI, - B KopHnx 6rrcrpoe 3Haqr{TeJrbHoe Hapacrzlur,re coAepxaHr.It BrrepBhle qacbr nocJre noBrrltreHr,r{ coAepxaHr,rr MerarrJra B cpeAe r,r 6onee MeAneHHoe u cna6oe erlocneA).Ioquft nepuo4, a B JlrIcrbfx - cyuecrBeHHo 6oree cna6oe rroBbrrrreHr.re coAepxaHr.rs,Hatll,IHarouleect roJIbKo nocJle 24'aacon uury6arlvu. 3rz pesynbrarbr Bax(Hbr, T.K. oHI,r He roJrbKoyBenI4qI,IBaroT HaIITU 3IJaIIprs, O [OCTy[neHI{}I TtXeJrbrX MeTaJIJIOB B pacTeHprs., IJO) [pexAe Bcero,cocraBJrflror ocHoBy ecefi nocre4yror{efi pa6oru aBTopa.

Conepruenno seo6xoAllMbl6ruru lr orrpeAeneHll-fl oBoAHeunocrlr JrrrcrbeB r.r coAepxaHr4txropo(pznna I4 KaporLIHoI,I,uoB B Jlllcrbtx. O6napyNeno 6rmo, qro rpu yBerr.rqeHHofi.uo 50 vncMKoHIIeHTpaqura cynr$araMep;z' B cpeAe rrpor{cxoAr4Jro 3aMerHoe cHrDreHrle coAepxaHr4r B Jrprcrbrxxropo(pnnna avKaporr4uoldAoB y)Ke rlepe3 24qaca eosAeficrerEs, TofAa KaK coAepxaHrze xnopo(pnnnab gocronepHo cHr4)r(aJrocb roJrbKo K oKoHqaHl,rro orlbrra. Xalr, oAHaKo, rrro 3Tor $arr ne o6cyx4eHmt6o c rIo3rIIIuu Brkrfl:nvts. MeAI4 Ha rlyrrl 6uocunrega pa3Hbx $optur xlopoQumla, rru6o c rro3r{ur.rupa3Hofo Blrvrflli.ns. MeAri Ha AerpaAarrlrro grr{x (boprur. Orcyrcrnne KaKoro-Jru6o 4eficrnus uoHoB MeAr4Ha ypoBeHb MarcuMulJlbuoro (<reunonoro>) KBaHToBoro BbrxoAa Sorocucrenarr II (Fv/Fm) rroKa3€uro,qro Aa:Ke r{epe3 72,aaca upe6rmaHuxpaclenuit B cpeAe c [oBbrrtreHHbrM coAep)r(aHr.reM LroHoB MeAr.rne 6rIrto AecrpyKTLIBHhrx HapyrrleHfifi n pa6ore peaKrlr4oHHbx rleHTpon Sotocunreaa.Bnuxnr,re MeALr, [ocryrraloqeft s nosrilleHuoM Konr,rqecrBe B pacreHvflTrpla yBenuqeHr,ru eecoAep)KaHI,It B cpeAe, Ha 3Kcnpeccruo ptAa reHoB, rroreHrlr{i}JlrHo cnoco6Hbrx yMenbrur{TbToKcr4qecKoe 4eficrnue rrxeJrbrx MeraJrJroB, - gro uau6oJree BaxHuur qacrb pa6orhr 3lo6uua I4.E. BTKaHf,x KopHt npu r,rs6rrroKe MeAu B cpeAe yxe B TerIeHLre rrepBbrx 3 qacoe orrbrra ua6lroAalocr 5-10-rcpauroe [oBblrrreHr{e co.{epxaHr,rr rpaHcKplrrrroB rpynnbr raK[x reHoB. 3ro 6ulu rcHbr xenaropoBrrxeJrbrx MerirJrJroB - (puroxeraruncvruTass PCSI, MeraJrJrorra[epoHa HIP06, a raKxe reHbrTpaHcrroprepoB, a LrMeHHo MRPI u MRP3, BoBJrerreHHbrx B TpaHcrropr KoMrrJreKcoB MerirJrJroB c

Suroxerarvrnavtvr, YSL2, ocyrqecrBnrrorqefo rparrclopr KoMnJreKcoB MerarrJroB c HrrKorEaHaMLrHoM,w ZIP5. flocne HaqaJrbnofo rroBbrrrreur4r coAepx(aHr4e rpaHcKpr,rlroB gTr.rx reuoB cHr{)K€urocb, T.e.BO3paCTaHI,Ie ypOBH{ SKCIIpeCCUI4 TIppIXOAI4JIOCb Ha nepBhre rracbr O[brTa, KOrAa cKopocTb yBenr{qeHr,rflcorepxaHprr MeAr4 B TKaHrx Kopnr 6rrna MaKcLtMarrbnofi. B cryqae AByx reHoB, reHaMeraJrJrorr.roHerrHa MT2b r{ reua MerarrJrorlalepoua CCS, excnp eccvllr Bo3pacrana qepe3 72 qaca u 24qaca, coorBercrBeHHo.

B rransx rvcra B ycnoBltsx zs6rrrKa MeALI B cpeAe ycuneHr,re gKcnpeccura ua6moAaloctToJrbKo 4rx 3 renon rag o6rqero qI{cJIa 17 nccneAoBarlHbrx feHoB, a krMeHHo reHa rpaHcrroprepaN RA MP 4, rena MeraJrnomanepoH a C C S lr reua MerarrJrorlrouer{Ha MT I a.

BaNnocrr 3Tux AaHHbx o6ycnonneua Bo3MoxHocrblo Ha rrx ocHoBe c rloMoilIbro resnofir4HxeHeprar4 yBenr,rtrr.rBarr ycroftvrEnocrb pacreur.rfi x 4eficrnulo rnreJrbrx MeriurJroB.

I4nrepeurrre pe3ynbrarbr [oJrfreubr rrpu u3freHnr,r rrrop(porornqecKnx uslaeueHr.rfi ropnenofiCLIcTeMbI pailca rroA BJIIIIHPIeM MeALI lI qLIHKa IIpH Bo3pacTAHvIV IIX KOHIIeHTpaquu B cpeAe. Eruoo6napyNeno, rITo ltpu yMepeHHoM r'rg6rrme ]IoHoB MeA]I r{ 3Harrr{TeJrbnol,r us6rrrKe r,roHoB rIrlHKarrpor4cxoArT 3aMeTHbre rlBMeHeHr,rx rraop$olorr4u KopHr, - oH yKopaqvr&aJtgg, yTonrqanc{, aKoJrr,rqecrBo 6oxonrrx xopneft Ha eAlrHr.rqy AnuHbr KopHfl 3HaqureJrbHo yBenrdqraBaJrocb. IIpu erou,npr4 r,rcnonngonaunofi n 6oruunncrBe onbrroB KoHrleHTpaquu 50 rrrnM ueAra B cpe.qe, r.r3MeHeHr4trrrop(ponoruu xopuefi He ua6nroAaJrocb, Ho rrepe3 72 qaca ilpoucxo.uuJro orqerJrlrBo Br,rAr,rMoerroBpexreHue rranefi KopHr.

C norrloutro Apyroro rroAxoAa, a r.rMeHHo, no cuoco6nocrlr KJreroK florurorrlarb Kpacr.rrenb

3saHc curtuit 6ruo orleHeno [oBpexAarorqee leftcrnlre MeALI ]I qI,IHKa ua ueu6palrbl KJIeroK KopHfl.

IIpz ntrcorofi ronqenrparlru{ MeAr{, 50 rvrxM, yxe qepe3 I qac na.ruHaJrocb rrponr4rHoBeHlle

Kpacr{TeJur B TKaHlr KopHJI B 3oHe pacrgxeHprt, a 3areM yxe qepe3 24'aaca [orJrorrleHr,re KpacureJul,

xapaKTeprc)Torrlee rroBpexAenue ueu6paH KJIeroK, paclpocrpaHslocb Ha BcIo rny6nuy KopHt.

Hanporue, rrplr WepeHHoM lrs6lrrre MeAu 3aMerHoe HapymeHr,re rleJrocrnocrra uevr6pan orMerranocbToJIbKo K oKoHqaHI,Iro orIbITa. llpu 4eficrBllrl qnHKa ronbKo qepe3 4'aaca cna6o oKparrrraBaJrr{cb 3oHbrpacrlxlKeHlla n g[(b$epeHrI]IpoBKLI, a 3areM, ycvrrvBancb K KoHrIy orrbrra, [por{cxoAr,rnorasbr.IparerbHoe noBpexIeHLIe ueu6pan KJreroK pr,BoAepMbr; rrpn gToM,upyrrle KJrerKrr KopHtlpaKTl,rqecKlr He nofJrorqiurH KpaclrreJrb.

O.{eHr rroJre3Hbre pe3ynbrarbl 6rmu rronfreHbl 3ro6znrna I4.8. npu lr3MepeHrlf,x coAepxaHr{sna6ulurrur <bopt MeAu I4 IILIHKa B TKaH{x KopHr upn ua6srroquoM coAepxaHr{Lr sTlrx MeriurJroB BcpeAe. 3ru u:rraepeHl,It rpoBoILInLIcb c [oMolrrbro MeAb- rr rlr{HK-crrequ(pn.*rrx (fnyopecqeHrHbrxxpacurerefi Phen Green SK ra Newport Green DCF, coorBercrBeHHo. B npracyrcrBr,rr.r KaK3HarI[TeJIbHoro, TaK I{ yMepeHHoro ug6rrrxa MeAu orMeqanocb Bo3pacraHl,re coAepxarrus ta6unbHbrx

$opu sroro MeraJIJIa eo scefi ToJIITIe KopHt ylxe B [epBhre qacbr onbrra, rrprrqeM 6olee 3naqr{TeJrbHoerlppl BblcoKofi xonqeHTpallura MerarIJIa. 9epes 72taca, cy.Ar no ypoBHro (frryopecqeuqnpr Phen GreenSK, xonr.ruecreo ra6urbHbrx $op,ur Bo3pacrano n 10 pa^:.

B upucyrcrnrara rag6rttKa III{HKa y)Ke B rrepBbre qacbr onblTa orMeqzurocb cyilIecrBeHuoeBo3pacraul4e coAepxaHus. ll'a1wtsttrrx (popu qI,IHKa B KJIerKix pu3oAepMbr, oco6euuo 3itMerHoe KoKoHqaHLIlo orrblTa. Oco6esno 6rura 3aMerHa QrryopecqeHrlnr B KnerKilx pr43oAepM6r B rrpeAenaxyronrrreHHofi soHrr BcacbrBarrfir;3a npeAeralrN grofi sonu (pnyopecrlenrlr.rr 6rura cyrqecrBeHHocra6ee. CreAosareJrbHo, r,rMeHHo prrcoAepMa B KopHe 6rrna uecrou r.rs6r,rpareJrbHoro HaKonneHr4tnara6onee noABr{xHbrx Qopr AaHHoro Mer€urJra, qro BeposrHo, r,r [pr{BoAr,rno x nau6o;reeBbrpDKeHHoMy Hapymenlrrc qenocTHocTr.r nreu6pan KJreToK prrcoAepMbr.

flocromry rnyrarLroH - gro BaxHrrfi roirruoneHT 3arrlr{Tubrx Mexa.Hr.r3MoB pacrrarelluofiKJIeTKI{ upla geficrevrvr:oa Hee rtxeJlhlx MeriIJIJIoB, I4 oAHa rlrc ero OynKrlrafi cocror.rr B rrprMoMCBx3bIBaHLII,I HOHOB 3TI,Ix MeT€IJIJIOB, BIIOJIHe JIOIIIIIHO, .ITO 3IO6uU IrI.E. npe4upr4Htn pa6Ory nOIl3freHplro rofo, KaK B ycnoBu.sx ug6rrrra MeAr{ I,I qI,IHKa MeHserct B KJrerKax ropneftBHyrpr{KneroqHoe coAep)KaHr{e BoccraHoBJreHHoro fnyrarr.roHa.I4ccneloBaHze [poBoAlrnocb cr{crroJlb3oBaH}reM $nyopecqeHTnoro Kpacr4TeJrr MoHoxJropo6zrr,rana. Anrop noKa3aJr, rrro rrpuo6pa6orxe r<opnefi xJropriAoM pryru An-fl 6roK[poBKr.r SH-rpynn 3aMerHa{ SlyopecqeHquxKpacI{TeJU{ B KonTpone norrrkr IroJIHocrbIo ucqe3iura. 3ro o6ecneqr{BaJro AocroBepuocrb rronfraeMbrxB OrrbrTax pe3ynbTaToB.

Buro o6napyxeno, qro coAep)KaHue BoccraHoBJIeHHoro rnyraruoua B rrptrcyrcrBr.rur B cpeAe50 urM KourleHTpaqrrr{ r,roHoB MeAr{ pe3Ko cHr.rxanocb y)Ke B rrepBbre qacbr Bo3Aeficrnux, a lcoKouqaHrrro orrbrra BoccraHoBnennrrfi rJryrarlroH B KopHe [paKT[qecKlr orcyrcrnonal. llplryMepeHHoM ug6rrrxe voHoB ue4u,10 rurM, cyAfl no $nyopecqeHrlr.rlr KoMrrJreKcon 6unaau-SG, narreKce KopHfl rrocne 72,]i,;cos orrbrra coxpaHrnocr 6oree 20o/o ot coAepxarrus BoccTaHoBJreHHorofnyrarr.roHa B KoHTpone, a B 3one BcacbrBaHr,rs - 6onee 50o/o.

B ormr.{ue or MeAH s srrcoroft KoHrIeHTpaqLrH, rIrrHK B 3HatlrlITenruo 6omruefi xouqeurpaquraeoo6qe He Bbr3brBaJr cHr4xeHlrt coAepxaHnfl BoccraHoBJIeHHoro rnyrarl4oHa B arruKansnoft .{acrl4

KopHr. B soHe BcacbrBaHlrr ero coAepxaHl,Ie yMeHblrraJlocb raKxe He3HaqHTeJrbHo. Anrop AenaerBbIBo.4, uro BoccraHonneunsrft fnyrarr4oH flBrflercg, oAHI,rM r,r3 ocuoBHbnr xenaropoB r,roHoB MeIr{ BKJrerKax KopH{ palca, Ho He r{rpaer noAo6uofi ponra B cnf{ae uoHoB rIr{HKa.

flonaurrro npruofi orIeHKr{ coAepxaHlr.fl BoccraHoBJrerrnoro rn}rarrroHa, 6rrno orrpeAeneno

4eficrnrze MeAlr r{ qr.rHKa B rroBbrrrreHHbx KoHIIeHTpaq}Ifi Ha coAep}Kaukre B KJrerKax KopHr o6uero u

oKr4cJreHHoro fnyrarl4oua. Kar rr B cnfrae onpeAeneHlt{ coAepxaH}Ir r.rrxeJrbrx MeruIJrJroB, AaHHbIe ocoAep)KaHr,rrr B TKaHrx o6ulero u oKrrcJreHHoro rJryTarllona I,IMeror caMocrof,TenbHyro rleHHocrb.Bne4enue B cpeAy LroHoB MeArl B nrrcorofi lr yMepeHHofi rouqenrparlr4rx rrpr4BoAlrno K Bo3pacraHl{IocoAep)KaHr4s oOqero rnyrar[oHa, vr B To xe BpeMrI K yMeHbIxeHI4ro crenenr4 oKI4cJIeHI4t efo [yna.

flpu srou coAepxaHne o6uero uryrarr,roHa n o6orEx cJlyquux yMel{brrranacb B rrepBsre 4uaca,uToJrbKo 3areM rrpolrcxoAr4n yKa3aHnbrfi pocr. Anrop 3aKrroqaer, qro, rrocKonbKy Aeftcreue rroHoBMeAr4 He npr4BoAr,rno K cr{uxeHr{ro co.qepxaur{.s B KrerKax KopH.f, o6uero rnyrarr{oHa, a raKxe HeyBenr,rqr.rBano creneub oKncJreHLr.r efo rryJla, TorAa KaK oKpuII[I{BaH}Ie MoHoxnopo6uuanorrtcBl{AereJrbcrBoBaJro o6 oqegr 3HarIr.rreJIbHoM yMeHbIIreHI{Lr coAepxaHxJl BoccraHoBJIeHHoro

4

fnyrarl{oHa, To IrocJIeAHee MorJIo 6uru pe:ynbraroM pacxoAoBarrrrs BoccraHoBJreHHofo rnyraruoHana o6pasonaHr4e KoMUreKcoB c LrouttMrl MerirnJra.

.{uuauzra coAepxanl{s o6qero rnyrarlloHa r.r creneHrr oKr.rcJrenr4r elo nyna B rrpracyrcrBr.rrirus6rrma qlrHKa 6rua rroxoxa Ha raKoBylo B npl,Icytcrsr,ru ug6HTra voHoB MeAH.

flocronrxy B KaqecrBe oAuofi LI3 ocHoBHbD( rrprrrrr.rH [oBpexAenux ueu6paH npu ruo6rrxcrpeccoBbrx nosAeficrnl4rx paccMarpuBaror [epeKr{cHoe oKficneHrre JrlrrrrdAoB (IIOJI), To aBTopoM6smo zccleAoBano BJIIIIHI,Ie MeAI{ }I qLIHKa Ha gror fipoqecc. B pa6ore 6rut racuo.rrb3oBaH Kpacr4Tenbimage-iT Lipid Peroxidation Kit, ilprl oKparrTvIBaHvIH KorophrM rro lr3MeHeHr,rro coorHorrrenr.rr Mexqy(pryopecqenqr.refi e sereHofi o6ractu, coorBercrByroqefi oKr{cJrenuofi Sopnae Kpacr{Ter-s, u xpacrofio6lactn, coorBersrByrorqefi BoccraHoBrennofi $oprvre, MoxHo 6rmo cyArfTb o pa3Blrrr.ru ilOJI, - pocrcoorHorrreHr.rr o3naqaJr Bo3pacraHr,re ypoBur IIOJI.

BeeAeHue B cpeAy Bblparru,IBanus50 uxM CuSO4.ne srrsrrsaJro rroBbrurennr ypoBHs ilOJI sTKaHtx KopHs rro cpaBHeH]Iro c KoHTpoJreM. flpu yuepeHHoM rag6rrme MeAr4 B cpeAe ypoBeHb nOI eKopHe, ecnu cy,ql4Tb Iro cooruolreHus SrryopecqeHrlr{Lr B genenofi u Hpacnofi o6nacrsx crreKTpa,ocraBaJlct [paKTI{qecKIa HeI{3MeHHbrM B xoAe Bcero orrbrra. O4naxo 3Harrr,rreJrbHoe yc[neHue

$ryopecqeHlllll4 oKplcJreHHofi (poprr,rrr KpacI,ITeJUr na6mo4zulocb qepe3 24'nca orrbrra Bnepu$epuuecxofi qacrtr KJIeroK, T.e. B gone ueu6parr. YcuneHlre crrrHaJra n gelenofi sacrvr crreKTpana nepn$epuu KJreroK oco6enno 6ruro 3aMerHo B 3oHe BcacbrBaurrr qepe3 72 taca,wocBl4AerenbcrBoBiulo o6 ycunenuu I{HTeHcvtBHocrIa neperr,rcHoro oKr4cJreurrr Jrr4nI4AoB B rrp[cyrcrBlrr4LroHoB MeAr4 r.rMeHHO B yMepeHHO rroBbrrrreunoft KoHrleHTpaqun. Anrop lpeAnonoxlrJr, qTo

cHI{xeHrIe I{HTeHcI{BHocru IIOJI rrpld BhrcoKofi r<onr{enrpaqrrlr MeAr.r B KopHrrx MoxHo o6rscHrampe3KI4M oHLIIKeHLIeM coAepx€Hl,I.f, B KrerKax KopHr BoccraHoBJreHHofo rnyrarrJoHa, ua6aro4an[rr{Mc{B groM cJrf{ae, rrro rrpr{BoAr,Ino K HeBo3MoxHocrI,I BoccraHoBJIeHLIt }roHoB Cu'- 4o Cu* naIIJIIBMzUIeMMe, a rrplr ]MepeHHoM usOrrtxe MeAlr B KJrerKax KopTI vMeJrcfl Heroroprrfi nylBoccraHoBJIeHHoro rnyrarl{oHa p.Jrs. BoccraHoBJreHus lroHon Cu'- Ao Cu- Bo3MoxHo, I4 TofAaua6nroAaerc . ycvnenvr.e IIOJI.

llpI,r 4efictsuu ug6rrrra qlIHKa K oKonrraHr4ro orrbrra B TKaHsx arreKca KopHs tr B 3oHeBcacbrBaHrrf, ypoBeHb IIOJI ocraBancr nplrMepHo Ha ypoBHe KouTponbHrm pacrenzfi

B mane 4 <O6cyN4eHI,re) aBTop anaJrr43krpyer Bcro coBoKynnocrb rronyqeunbrx AaHHbD(,rrpr.rBneKar AarrHbre nrrreparypu. flpu groM, xorr uraBa pazduta:rrarracru, B Koropbrx o6cyx4aercrBIrvIflHvIe MeAu I{ III4HKa Ha OTAenbHbIe I{3fIeHHbIe tBJIeHLI.fl, aBTOp ilpOCneXLIBaeT CBt3b MeXAypa3HbIMI{ s$(perrarr,ru, purcyfl AocraroqHo rleJlbHyro KaprnHy AeftcrsIas gTux rs]Kerbrx MeraJrnoB.Haueqeuo rd npoAonx(eur,re HeKoropbrx uccne4onaur,rfi .

B <3arnro.reHr{r,r) KoporKo rroABoAr{Tc.f, }rrof nceft npo4elanuoft pa6oruCyrqecrnenHble pe3ynbrarbr, rroJlfreHHbre B xoAe rrpoBeAeHHoro IaccneAoBalrrfl, orpaxeHbr B

BbrBo,4ax Ar.rccepTarlr,rH.llo nponegeuuoft pa6ore r4 rro reKcry A]rcceprarlr4r,r MoxHo cAenarb orreHb HeMHoro garr{e.raHr.rfi.

B qacrnocur, HenoJrHo onncaHa MeroAHKa o[peAeneHrrfl coAepxanux o6qero rr oKr{cJreHHorofJryrarlroHa. B noAuucrr K plrc. 10 lle yKa3aHo, B Kopnf,x LIJITI Jlr{crbflx rroKa3aHbr ypoBHr,r gKclpeccprr4

reHoB. B noAnucu K pr4c. 25 lr,anucamo, rrro Ha HeM rrpeAcraBneso <CoorHorrreurle. ...>>, a ocb opArdnarobo:naqena KaK (yponeur $nyopecrleHrlr4u>>. B rexcre repenyraHbr HoMepa plrcyHroB 29,30, 3I. BTeKCTe Ar{CCepTarIUI4 BCTpeq€UOTC{ [pOCTOperlAs., a TaKXe KaJrbKr4 LTHOCTpaHHbD( TepMHHOB rrpr4 llilrv'l{ril4pyccKr{x gKBrrBaJreuros. KaK rroxeJraHlle Mo)KHo orMerllTb ueo6xoAuruocrr 6oree AeranbHoro aaurvaaBo3MoxrHbrx Mexanr.I3Mon ua6rnoAaeMbrx eQQerton.

MoNHo 3aKJrrorrr,rrb, rrro Ar.rccepraqr,roHHar pa6ora 3no6zna I4. E. necuorpr Ha prA orMerIeHHbIXHeAoqeroB, rrpeAcraBrser co6ofi BaxHoe r{ oqeub }rHTepecHoe r{ccneAoBarr}re. BrrsoArr

Ar{cceprauraonnofi pa6orbr o6ocnosanbl Ii coorBercrByror gKcnepl{MeHTzlJrbubrM AaHHbM. AnropeQeparnoJrHocrbro orparaer coAep)KaHlre ,{[cceprarluu. Marepvurbr.qr,rcceprauraonnofi pa6oru, B ocHoBHoM,lpeAcraBneHhr B y)r(e ony6rurronaHHbrx pa6orax rr HeoAHoKparHo AoKJraAbrBilJrr.rcb na poccuficKrrx rIMe)r(AyHapoAHbrx ron(f epenquflx vr cbe3.4ax.

[r,rccepraqwsl4trr,u EereHreeuqa 3no6rHa rlpeAcraBnser co6ofi 3aBeprrreHHyro HayrrHo-llccneAoBarenbcKylo pa6ory Ha aKTyiuIbHyIo reMy. floryrennrre pe3ynbrarbr BHocflT BKJTaA BrroHr4MaHr.re MexaHr,r3MoB Blrvrsllus. Tr)r(eJrhrx MeTaIInoB Ha pacTeHl4r.

Pa6ora IroJIHocrbIo coorBercrByer BceM rpe6onaura.f,M rl. 9 <flonoxeHr4r o rprcyx,{eHr.{r{

f{eHbrx creneneft>, yrBepx,qeHuoro flocranonneHrreM llpannrenrcrsa Pocclrficlcofi @e4epaqrau or24.09.2013 r. N 842, [peAbrBnf,eMhrM K Kar{.u.rAarcKrrM Ar.rccepraqr{oHHrnra pa6oral,r, a ee aBTop3no6un I4.E. zac;tyrlrBaer np[cy]KAeHHTI yrleHoft creuenu Kar{Ar,rAara 6raonorH.recrlrx Ha}.K rrocfleqr4aJrbuocrra 03 . 0 1 . 0 5 - $nsuoJrorur n 6pt oxraurn x pacrelauir.

3an. Jla6oparopr.refi Q orocnurerr.rqecKoro onerrpoHHoro rpaHcrropra@e4epa;ruroro rocyAapcrBeuHoro 6roANeruoro yqpexAeHrdr HayKr4lrlucrr.rry.ra $ynaartresriurbHbD( npo6rervr 6uonorzuPoccufi ucoft arcaAeunr.r HayK

AoKTop 6zonorzqecKr,rx HayK(cnequamuocrb - 03.00.02 EraoSzsuxa)

I1 rnnapx 2016r .

I4saHon Eopnc Huroraeerq@e4epa-nrnoe rocyAapcrBeHHoe 6roAxeruoe frpexAeHr,re HayKr{

I,Iucruryr (fyuAaueurilJrbHbD( upo6nena 6raororuvPoccuficrofi axa4evrnlr HayK| 42290 r. flyruluro, y tr. Llucruryrcrcan 2ren. 8-496-77336073I. [OrITa:

l4nauon E.H

'" j,:1,

S;a.r<:rq i f i ;ntu illxcruryra $y*ga ucr ra;turir,il r,?Lt$*,{s 6qot