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2018 HKCBEES BANGKOK CONFERENCE
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2018 International Conference on
Agriculture, Food and Biotechnology
(ICAFB 2018)
January 24-26, 2018
CHATEAU DE BANGKOK
Sponsored and Published by
Indexed by
www.cbees.org
2018 HKCBEES BANGKOK CONFERENCE
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Table of Contents
2018 HKCBEES Bangkok Conference Introductions 6
Presentation Instructions 7
Keynote Speaker Introductions 8
Plenary Speaker Introductions 14
Brief Schedule for Conference 17
Detailed Schedule for Conference 19
Session 1 21
B3004: Use of Unmanned Aerial Vehicles in Crop Protection
Dr. Piyush Mishra, Vikas KT, Madhu KS, Babu KS, Dr. Mallikarjunappa S., Dr. L
Venkatakrishnan, S Sudhakar and P Suriyanarayanan
21
B0026: Economics of Big Data in U.S. Agriculture
Mitchell Morehart
22
S1001: Fish Allergenicity Modulation towards the Production of a Low Allergen Farmed
Fish; a Proteomics Approach
Pedro M L Rodrigues, Denise Schrama and Cláudia Raposo
23
S2001: Review of Environmental Sustainability Assessments of Aquaculture Systems:
Main Findings and Outlook
Florence Alexia Bohnes, Michael Zwicky Hauschild, Jørgen Schlundt and Alexis
Laurent
24
S0003: Research on the Demand and Availability of Formal Loan in Aquaculture
—Taking the Flatfish Farmers as an Example
Zhang Yingli, Yang Zhengyong, Zhang Di and Shen Xin
25
B4001: Storage Temperature and Packaging Treatment on Antioxidant Capacity of
Ciplukan (Physalis peruviana L.)
Aldila Putri Rahayu and Deffi Armita
26
B0020: Early Feasibility Study on Capparis Production and Processing in Hamedan
Province in Iran
Mahdi Reyahi-Khoram and Reihaneh Reyahi-Khoram
27
B0043: Optimization of Ultrasonic Assisted Extraction (UAE) of Anthocyanins from
Black Glutinous Rice and Evaluation of their Antioxidant Properties
Anjali Maniyam Pariyarath, Anil. K. Anal and Muhammad Bilal Sadiq
28
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Session 2 29
B0037: Microcrustacean Moina Macrocopa: An Important Feed for Aquaculture and a
Potential Bioindicator for Heavy Metals Contamination in Aquatic Environments
Chanya Phromchaloem, Amonrujee Nakphlaiphan and Thanit Pewnim
29
B0009: Trehalose Association for Drought Tolerance in Wheat (Triticum Aestivum L.)
Germplasm through SSR Markers
Muhammad J. Iqbal, Naureen Shams and Amer Jamil
30
B0038: Effect of Plant Growth Promoting Rhizobacteria on Growth and Yield of 2 Red
Lettuce Cultivars
Wisnu Eko Murdiono, Koesriharti and Palupi Wulandari
31
B0022: Telemetric Lactic Acid Monitoring System for Real-time Assessment of
Exercise Intensity
Keigo Enomoto, Takahiro Kurose and Hiroyuki Kudo
32
B0013: Enhances Mitochondrial Function in Human Neuronal SH-SY-5Y Cell Line via
3D Bioprinter
Ming-Chang Chiang
33
Session 3 34
S0006: Application of Remote Sensing and GIS for Water Environment Suitability
Evaluation Around Lampung Bay and Hurun Bay, Indonesia
Andiyanti Putri Estigade, Ariani Puji Astuti, Arief Wicaksono, Dea Nadia, Mousafi
Dimas Afrizal, Muhammad Hilmy Aziz, Tika Maitela
34
B0010: Exploring a New Way to Create Pectin Hydrogels
Havazelet Bianco-Peled, Irit Ventura and Lena Neufeld
35
B0029: Thick Activated Carbon Sheet Electrode and Hydrophobic Organic Matter
Improve the Performance of Microbial Fuel Cell
Naoki Hayashi, Dang Trang Nguyen and Kozo Taguchi
36
B0027: Optimum Conditions for Titanium Oxide Thin Film on Dye-Sensitized Solar
Cells Using Organic Dye Sensitizer-MK2
Yoshiki Kurokawa, Dang Trang Nguyen and Kozo Taguchi
37
B0039: Impact of Complimentary/Restricted Irrigation on Wheat Yield and Its
Components
Shahryar Sasani, Rahman Rajabi and Mosayeb Novroozi
38
B4003: Contamination of Mercury around the Artisanal and Small-Scale Gold Mining
Area and the Potential of Sisal (Agave sisalana) for Phytoremediation of the Mercury
Contaminated Soil
Sachiko Takahi and Kokyo Oh
39
Poster Session 40
S1002: Effects of Histidine, Threonine and Tryptophan Supplementation on the
Performance of Gilthead Seabream Using 2D-DIGE.
40
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Denise Schrama, Rita Colen, Sofia Engrola, Tomé Silva, Pedro M.L. Rodrigues
S4001: Atypical Furunculosis Caused by Aeromonas Salmonicida Subsp. Masoucida
Causes Mortality in Farmed Korean Rockfish (Sebastes schlegelii)
Do-Hyung Kim and Chan-Il Park
41
S4002: Distribution of CD83+ Cell of Olive Flounder (Paralichthys olivaceus): Gene
Expression after Viral Haemorrhagic Septicaemia Virus (VHSV) induced Infection in
Fish
Do-Hyung Kim and Chan-Il Park
42
B0008: Synchrotron Radiation for the 3D Anatomical Structure Analysis of Bio-sample
ONSEOK LEE
43
B1005: Numerical Analysis according to Evaporator Type
Dae Yeong Kim, Moon Ki Kim and Hong Sun Yun
44
B0014: Phytase Production by Grifola Frondosa and its Application in Inositol-enriched
Solid-state Fermentation brown Rice
Shih-Jeng Huang, Chieh-Han Chen, Zih-You Chen, Zhi-Wei Lin and Shu-Yao Tsai
45
B0016: Effect of Lateral Displacement of the Papillary Muscles on Functional Mitral
Regurgitation
Woojae Hong, Soohwan Jeong and Hyunggun Kim
46
B2005: Tissue Distribution of Copper in Mice Following Exposure to Arsenic in
Drinking Water
Younghee Kim, Jiguk Kim and Kisok Kim
47
B0017: How to Predict Gait Speed and Ground Reaction Forces using Foot Pressure
Sensors
Chang-Hyun Choi, Sun-Ok Chung and Joung Hwan Mun
48
B0021: Near-infrared Spectroscopy Can be Used in Evaluation of Flesh Texture
(Crunchiness) and Water Content (Juiciness) of Apple Fruit
Jian-Zhi Chen and Sheng-Hao Tseng
49
B0024: Study on Water Quality in Tributaries of the Han River in Seoul
Sang-Hoon Lee and Hong-Kyu Ahn
50
B0025: Study on the Application of the Stream Environment Assessment Technique for
Stream Restoration - Focusing on 30 Streams in Seoul Korea
Hong Kyu Ahn and Sang-Hoon Lee
51
B0028: The Impact of PDDA in CNT Counter Electrode on the Conversion Efficiency
of DSSC
Yoshiki Kurokawa, Dang Trang Nguyen and Kozo Taguchi
52
Shallow HVs and Geothermal Energy for Heating Purposes in Aquaculture
Hans-Uwe Dahms
53
B4004: Biomimetic Tactile Information Processing for Surface Roughness
Discrimination
Qin Longhui and Zhang Yilei
54
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B0019: Effects of Low Soil Temperature on Antioxidant Enzyme System of Rice under
Drip Irrigation
Jun Zhang, Peng Dong and Changzhou Wei
55
Listeners 56
One Day Visit 57
Conference Venue 58
Note 59
Feedback Information 63
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2018 HKCBEES Bangkok Conference
Introductions
Welcome to CBEES 2018 conference in Bangkok, Thailand. The objective of the Bangkok conference is to provide a platform for researchers, engineers, academicians as well as industrial professionals from all over the world to present their research results and development activities in Agriculture, Food and Biotechnology and Sustainable Global Aquaculture.
2018 International Conference on Agriculture, Food and Biotechnology (ICAFB 2018)
Papers will be published in one of the following journals:
Journal of Advanced Agricultural Technologies (JOAAT, ISSN:2301-3737),
which is indexed by Ulrich's Periodicals Directory, Google Scholar, Engineering &
Technology Digital Library, Crossref and Electronic Journals Digital Library, et al;
International Journal of Life Sciences Biotechnology and Pharma Research
(IJLBPR, ISSN:2250-3137), which will be indexed by Embase (Under elsevier),
ProQuest, Google Scholar, Chemical Abstracts Services (CAS), Indian Science,
ICMJE(International Committee Medical Journal Editors), et al.
International Journal of Food Engineering (IJFE, ISSN: 2301-3664), which is
indexed by WorldCat, Google Scholar, Cross ref, ProQuest , CABI.
Conference website and email: http://www.icafb.org/; [email protected]
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Presentation Instructions
Instructions for Oral Presentations
Devices Provided by the Conference Organizer:
Laptop Computer (MS Windows Operating System with MS PowerPoint and Adobe Acrobat
Reader)
Digital Projectors and Screen
Laser Sticks
Materials Provided by the Presenters:
PowerPoint or PDF Files (Files should be copied to the Conference laptop at the beginning of
each Session.)
Duration of each Presentation (Tentatively):
Regular Oral Presentation: about 12 Minutes of Presentation and 3 Minutes of Question and
Answer
Keynote Speech: about 35 Minutes of Presentation and 5 Minutes of Question and Answer
Plenary Speech: about 15 Minutes of Presentation and 5 Minutes of Question and Answer
Instructions for Poster Presentation
Materials Provided by the Conference Organizer:
The place to put poster
Materials Provided by the Presenters:
Home-made Posters
Maximum poster size is A1
Load Capacity: Holds up to 0.5 kg
Best Presentation Award One Best Oral Presentation will be selected from each presentation session, and the
Certificate for Best Oral Presentation will be awarded at the end of each session on January
25, 2018.
Dress code Please wear formal clothes or national representative of clothing.
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Keynote Speaker Introductions
Keynote Speaker I
Prof. PAUL B. BROWN
Department of Forestry and Natural Resources, Purdue University, USA
Prof. PAUL B. BROWN’s research program focuses on aquaculture and aquatic animal nutrition. They
explore aquacultural opportunities for Indiana and other parts of the world and have worked with American
lobsters, spiny lobsters, hybrid striped bass, yellow perch, bluegill, largemouth bass, walleye and tilapia.
Their nutrition work focuses on new diets for established aquaculture industries and development of diets
for emerging industries. They have been working on modified diets for rainbow trout and Atlantic salmon,
as well as tilapia. New diets for yellow perch, hybrid striped bass and largemouth bass have been developed.
Much of our work has been focused on replacing fish meal in diets. The global supply of fish meal has been
at maximum sustainable yield for approximately 8 years, but demand continues increasing. They have been
successful replacing fish meal with soybean meal in diets for yellow perch, and hybrid striped bass and are
currently exploring use in largemouth bass. There is a need within aquaculture specifically and animal
nutrition in general to develop diets rapidly. They are currently exploring use of two new scientific
disciplines to understand nutrient needs and use of ingredients from diets. Combining metabolomics and
proteomics provides us with a more complete view of metabolism. They are also using the combination of
metabolomics and proteomics to understand flows of nutrients through food webs and applying this
approach to both aquaculture and natural settings.
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Topic: ―Sustainable Aquaculture: a Long Way to Go and a Short Time to Get There‖
Prof. Paul B. Brown
Purdue University, Indiana, USA
Abstract—Sustainability is a commonly used term applied to fisheries. Maximum sustainable yield (MSY)
has been one of the driving forces in harvest regulations for the past century or so. Sustainability has also
been used in reference to aquaculture, particularly as aquacultural production increased over the past 25+
years. Sustainable aquaculture development was considered as early as 1987-1989 as fisheries harvest
reached MSY. However, the newer definition, or vision, for sustainable development has not been fully
embraced in aquaculture production. At a minimum, sustainability encompasses three spheres,
environmental, economic and sociological, with sustainability occurring at the juncture of all three spheres.
New metrics have been developed that attempt to quantify disparate systems, such as food production
systems, and can be applied to various aquaculture production systems. Initial data developed indicate
feed inputs as one of the least sustainable components of food production systems, including aquaculture.
Dietary ingredients can be characterized by the amount of carbon dioxide (CO2) produced during
production, harvest, processing, storage and distribution. The Global Warming Potential (GWP, kg CO2/mt
product) differs significantly across commonly used dietary ingredients, decreasing significantly as we
move from fish meal (3500), to plant-based ingredients (437), and animal by-product ingredients (68).
Additional quantitative measures of sustainability include abiotic depletion (fossil fuel use, Sb equivalents),
acidification potential (PO4 equivalents), eutrophication potential (PO4 equivalents), human toxicity
potential (1,4-dichlorobenzene equivalents), marine toxicity potential (1,4-dichlorobenzene equivalents)
and cumulative energy potential (MJ). The water footprint, or water demand for food production, is another
quantitative measure that can be incorporated into developing models of sustainability. Preliminary data
indicates that water-based food production systems such as aquaculture demand significantly less water per
unit food production than terrestrial-based food production systems. The global aquaculture community
could benefit from an expanded definition of the term sustainability and application of the developing
quantitative measure of sustainability might identify seafood production systems as desirable for global
economic development goals.
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Keynote Speaker II
Prof. Ching Chang Lee
National Cheng Kung University, Taiwan
Prof. Ching Chang Lee is with rich work experience. Section Chief, Environmental Protection Bureau,
Department of Health(1986-1987); Section Chief, Environmental Protection Administration Government
of the Republic of China(1987-1988); Lecturer, Department of Environmental and Occupational Health,
College of Medicine, National Cheng Kung University(1991-1993); Associate professor, Department of
Environmental and Occupational Health, College of Medicine, National Cheng Kung University
( 1993-2007 ) ; Director, Department of Environmental and Occupational Health, College of Medicine,
National Cheng Kung University(1996-1999); He works as a distinguished professor in Department of
Environmental and Occupational Health, College of Medicine, National Cheng Kung University from 2007
to now. He is also a director of Research Center of Environmental Trace Toxic Substances, National Cheng
Kung University from 1999. Fellow of The academic of Fellows, ISIAQ. President of Taiwan Society of
Indoor Environmental Quality.
He got his Bachelor of public health- Department of Public Health, National Taiwan University in1980;
Master of environmental engineering - Graduate Institute of Environmental Engineering, National Taiwan
University in1982; Ph.D in Graduate Institute of Environmental Engineering, National Taiwan University
in1992.
His major fields of study include Risk assessment and management of toxic substances, Food safety and
hygiene, Endocrine disruptor exposure and health outcomes, Sampling and analysis of environmental and
occupational trace pollutants, Environmental and Occupational Health, Air pollution and control, Health
risk assessment and management of toxic chemicals.
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Topic: ―Phthalates Exposure Ans Health Outcomes of Pregnant Woman, Newborn,
Precocious Girl and Adult Men‖
Prof. Ching Chang Lee
National Cheng Kung University, Taiwan
Abstract— Phthalates are added to plastics to make them soft and flexible, to cosmetics as a vehicle for
fragrance, and to many other daily products, such as building materials, children's toys, and medical
devices. Phthalates are not chemically bound to these products and are released continuously into
foodstuffs and the environment. Because phthalates are ubiquitous in daily life, the potential consequences
of human exposure to phthalates have raised concerns in the general population. Phthalates, are
reproductive and developmental toxicants in animal models, and the ability of phthalates to penetrate the
placenta have been reported. Phthalates are considered anti-androgenic endocrine disruptors because of
their possible effect on animal gonads and reproduction. In addition, some studies have reported possible
antagonistic effects of phthalates on the thyroid gland in vivo and thyroid tissue in vitro. However, little is
known about those issues in humans.
In our first study, we found a significant positive correlation only between creatinine adjusted urinary MBP
and amniotic fluid MBP (R2 = 0.156, P < 0.05) in all infants and, only in female infants, a significantly
negative correlation between amniotic fluid MBP, AGD (R = .0.31, P < 0.06), and the anogenital index
adjusted by birth weight (AGI-W) ( R = .0.32, P < 0.05). Although the influence of prenatal di-n-butyl
phthalate (DBP) exposure on the endocrinology and physiology of the fetus is still a puzzle, our data
clearly show that in utero exposure to phthalates in general has anti-androgenic effects on the fetus.
In second study, we found all seven urinary phthalate metabolites in the CPP group were significantly (P <
0.05) higher than in prepubescent controls. Serum kisspeptin-54 levels were higher (P < 0.022) in the CPP
group than controls and were still significantly higher after adjusting for age (P < 0.03). There is a
significant increasing trend (Ptrend < 0.005) between levels of kisspeptin and the stages of puberty, and a
significant positive correlation between kisspeptin-54 and urinary mono-n-butyl phthalate (ng/ml: R2 =
0.251, P < 0.001; mg/g-creatinine: R2 = 0.109, P < 0.024). We found kisspeptin may promote the onset of
puberty in girls who are exposed to a high level of phthalates, especially di-n-butyl phthalate. And then,
The intervention strategies that we set up in this study were effective for reducing exposure to phthalates in
children. Handwashing and drinking fewer beverages from plastic cups were the most effective strategies
for reducing phthalate metabolites in urine, especially MBP and di(2-ethylhexyl) phthalate (DEHP)
metabolites.
In third study, we found an inverse association between urinary levels of MMP, MiBP, MEHP, MEHP%
and serum TT. The inverse associations were also found between urinary levels of MiBP, MBzP, MEHP,
MEHP% and serum fT. Urinary MBzP and MEHP% were negatively associated with a decrease in serum
INSL3. We also observed a strong inverse relationship between MEHP% quartiles and serum TT, fT, the
TT : LH ratio and INSL3. Ssignificantly monotonic trends in semen volume, sperm concentration and
motility were associated with increasing quartiles of INSL3 were found. In adjusted regression models,
increases in urinary phthalate metabolites levels were adversely associated with sperm concentration
(MBzP, MEHP and MEHP%), motility (MBzP and MEHP) and INSL3 (MBzP, MEHP and MEHP%). Our
data suggest that INSL3 secretion, reproductive hormone balance, and sperm production and quality might
be simultaneously adversely affected.
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Keynote Speaker III
Prof. Hans-Uwe Dahms
Kaohsiung Medical University, Taiwan
Porf. Hans-Uwe Dahms was born in Wilhelmshaven, Germany. He received his PhD and DSc
degrees in Biology from University Oldenburg, Germany. He is currently a Professor at the
Department of Biomedical Science and Environmental Biology in Kaohsiung Medical
University (KMU - Taiwan). His current research is equally concerned about Environmental
and Public health. This includes green technologies to monitor, remediate and sustainably
manage environmental and medical issues in aquatic systems.
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Topic: ―Antibiotics and Antibiotic Resistant Microbes in Aquaculture – Sources, Sinks
and Pathways‖
Prof. Hans-Uwe Dahms
Kaohsiung Medical University, Taiwan
Abstract—More than 5 million cases of hepatitis and more than 250 million cases of gastroenteritis and
respiratory diseases are reported annually worldwide. This indicates that infectious diseases are on the rise.
Reasons for this are an ever increasing world population and the ease of travelling that enhances the risk of
disease spread. An unprecedented rise of antibiotic resistance of most pathogenic microbes and viruses
provides another serious health risk. We show that the aquatic environment and aquaculture within
freshwater, brackish and seawater components provide a serious source of antibiotic resistant pathogens.
Here, some pathogens even get more virulent than in their human host. This way environmental health as a
branch of public health gets unexpected importance with several facets of the natural and man-made
environment that affects human health. Three basic disciplines generally contribute to the area of
environmental health: environmental epidemiology, toxicology, and exposure science. Information from
these three disciplines can be combined to conduct a risk assessment for specific physical, chemical, or
biological hazards. Such risk factors, separate or in combination, determine whether an exposure poses
significant risk to human health. This can in turn be used to develop and implement environmental health
policies that regulate chemical emissions, or impose standards for proper sanitation. This way
environmental health management can become a tool of preventive medicine. Preventive medicine as a
branch of public health attempts to prevent diseases such as infectious diseases, as opposed to disease
treatment. Just as public health focuses on a variety of physical and mental states, so do disease and
disability, which are affected by genetic predisposition, disease agents, life style, and environmental factors.
We will provide examples here from antibiotics and antibiotic resistance of human pathogenic bacteria that
were collected from aquaculture facilities in freshwaters, brackish and coastal waters. These are posing
threats to respective ecosystems and the food and drinking water safety of coastal populations. Possible
solutions to the antibiotics crisis are outlined here and discussed.
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Plenary Speaker Introduction
Plenary Speaker I
Assoc. Prof. Win Chaeychomsri
Faculty of Science, Kasetsart University, Thailand
Assoc. Prof. Win Chaeychomsri received a Bachelor‘s Degree in Biology, Master‘s Degree in
Tropical Medicine, and Doctoral Degree in Animal Biotechnology. He has been a lecturer in
Department of Zoology, Faculty of Science, Kasetsart University since 1994. He is an
outstanding lecturer in research and innovation. His current research interests are in the fields
of parasitology, immunology and animal biotechnology. He has authored books, published
more than 100 technical papers in various journals and conference proceedings. He gets 3
petty patents and 2 patents. These petty patents and patents involve the production technology
of freeze-dried crocodile blood that will be used as dietary supplement for health
improvement.
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Topic: ―Successful Innovation of Freeze-dried Crocodile Blood and Related Products as
Dietary Supplement‖
Assoc. Prof. Win Chaeychomsri
Faculty of Science, Kasetsart University, Thailand
Abstract—Among the earliest recorded use of crocodiles in Traditional Chinese Medicine (TCM) was the
application of blood for health care benefits. It has been reported that crocodile blood contains potent
antibacterial and antiviral properties. The Siamese crocodile (Crocodylus siamensis) is one of the medicinal
animal. This species is a freshwater crocodile native to Southeast Asia. The practice of consuming
crocodile blood for improving human health is found in many Asian cultures and traditions. Crocodile
blood capsule, a dietary supplement, is the world and Thailand‘s first crocodile blood product based on
research studies conducted by the Crocodile Research Unit, Department of Zoology, Faculty of Science,
Kasetsart University (KU), Thailand. This product is derived wholly from captive Siamese crocodiles blood,
containing 100% natural ingredients and no artificial preservatives added. Crocodile blood capsule contains
high levels of protein, iron, many minerals and vitamins. The large volume-collecting and freeze-drying
processes of Siamese crocodile blood were designed and developed. These freeze-dried blood products
were packed in capsule, testing for bacterial and heavy metal contamination. The results showed that
freeze-dried crocodile blood products have no bacterial and heavy metal contamination. In collaboration
with private sector, KU has made an effort to enhance clean and safe production processes at the acute,
sub-chronic, and chronic levels, and to study the efficiency of freeze-dried crocodile blood product, which
has subsequently approved by the Thai Food and Drug Administration (Thai FDA), Ministry of Public
Health, as a dietary supplement since 2008. The safety evaluation of freeze-dried crocodile blood was
performed in both sexes of Sprague Dawley rats. Freeze-dried crocodile blood was orally administered for
24 weeks. The blood ingestion had no effect on rat behavior and survival. The hematological values did not
reveal differences between the treated and the control groups. The biochemical values including alkaline
phosphatase (ALP), aspartate transaminase (AST), alanine transaminase (ALT), blood glucose, blood urea
nitrogen (BUN) and albumin were not significantly different among experimental groups throughout the
study time. The results observed in iron-deficient rats demonstrated the efficiency of freeze-dried crocodile
blood in increasing values of hemoglobin and hematocrit within the first 4 weeks of treatment and no
histological changes in intestine, kidney and liver after a 24-week treatment. These data suggest that the
freeze-dried crocodile blood has safety and efficiency in promoting hemoglobin and hematocrit values.
Thus, the freeze-dried crocodile blood can be used as food supplements in anemia patient.
Then, KU has permitted for using intellectual properties and transferred crocodile blood capsule production
technology to private sector under the brand ―Wynn‖ that has received certificate from Thai FDA as No.
13-1-03060-1-0001. The freeze-dried crocodile blood products are available in the market and many
consumers who suffer with anemia, cancer, AIDS and diabetes have told the research team that their health
conditions and problems are improved. Since insulin-like growth factor I (IGF-I) has been found in
Siamese crocodile serum and the ability of IGF-I to reduce fasting blood glucose levels is well recognized.
Recently, the crocodile serum has been developed for the patient with diabetes under the brand name
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―Jakhae Thai‖. In addition, the guidelines for collection of large volumes of blood from crocodile have
been developed with the goal to ensure the product quality, and also safety of the animals. Therefore, these
data demonstrate a good case in value-added crocodile blood product development. Most importantly, a
focus on quality management system would add value and help expand both local and export markets.
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Brief Schedule for Conference
Day 1
January 24, 2018 (Wednesday)
Venue: Lobby
Arrival Registration and Posters Collection 10:00~17:00
Day 2
January 25, 2018 (Thursday)
Venue: LAROSE
Arrival Registration, Keynote Speech, Plenary Speech, and Conference
Presentation
Morning Conference
Opening Remarks 9:00~9:10
Prof. PAUL B. BROWN, Purdue University, USA
Keynote Speech I 9:10~9:50
Topic: ―Sustainable Aquaculture: a Long Way to Go and a Short Time to Get
There‖
(Prof. PAUL B. BROWN, Purdue University, USA)
Keynote Speech II 9:50~10:30
Topic: ―Phthalates Exposure Ans Health Outcomes of Pregnant Woman,
Newborn, Precocious Girl and Adult Men‖
(Prof. Ching Chang Lee, National Cheng Kung University, Taiwan)
Coffee Break and Group Photo Taking 10:30~11:00
Keynote Speech III 11:00~11:40
Topic: ―Antibiotics and Antibiotic Resistant Microbes in Aquaculture –
Sources, Sinks and Pathways‖
(Prof. Hans-Uwe Dahms, Kaohsiung Medical University, Taiwan)
Plenary Speech I 11:40~12:00
Topic: ―Successful Innovation of Freeze-dried Crocodile Blood and Related
Products as Dietary Supplement‖
(Assoc. Prof. Win Chaeychomsri, Faculty of Science, Kasetsart University,
Thailand)
Lunch 12:00~13:30
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Afternoon Conference
Session 1 13:30~15:30
Venue: LAROSE 1
8 presentations-Topic: ―Agricultural
Science and Food Engineering‖
Session 2 13:30~14:45
Venue: LAROSE 2
5 presentations-Topic: ―Biology and
Biotechnology‖
Session 3 14:45~16:15
Venue: LAROSE 2
6 presentations-Topic: ―Environmental
and Chemical Engineering‖
Coffee Break 16:15~16:30
Poster Session 16:30~18:30
Venue: LAROSE 1
16 posters
Dinner 18:30
Tips: Please arrive at the conference room 10 minutes before the session begins to upload
PPT into the laptop.
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Detailed Schedule for Conference
January 25, 2018 (Thursday)
Venue: LAROSE 1 LAROSE 1
9:00~9:10
Opening Remarks
Prof. PAUL B. BROWN
Department of Forestry and Natural Resources, Purdue University, USA
9:10~9:50
Keynote Speech I
Prof. PAUL B. BROWN
Department of Forestry and Natural Resources, Purdue University, USA
9:50~10:30
Keynote Speech II
Prof. Ching Chang Lee
National Cheng Kung University, Taiwan
10:30~11:00 Coffee Break and Group Photo Taking
11:00~11:40
Keynote Speech III
Prof. Hans-Uwe Dahms
Kaohsiung Medical University, Taiwan
11:40~12:00
Plenary Speech I
Assoc. Prof. Win Chaeychomsri
Faculty of Science, Kasetsart University, Thailand
12:00~13:30 Lunch
13:30~16:15
Session 1 (13:30~15:30)
8 presentations-Topic: ―Agricultural Science
and Food Engineering‖
Session 2(13:30~14:45)
5 presentations-Topic: ―Biology and
Biotechnology‖
Session 3(14:45~16:15)
6 presentations-Topic: ―Environmental and
Chemical Engineering‖
16:15~16:30 Coffee Break
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16:30~18:30 Poster Session
16 posters
18:30 Dinner
Note: (1) The registration can also be done at any time during the conference.
(2) The organizer doesn’t provide accommodation, and we suggest you make an early reservation.
(3) One Best Oral Presentation will be selected from each oral presentation session, and the
Certificate for Best Oral Presentation will be awarded at the end of each session on January 25, 2018.
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Session 1
Tips: The schedule for each presentation is for reference only. In order not to miss your presentation,
we strongly suggest that you attend the whole session.
Afternoon, January 25, 2018 (Thursday)
Time: 13:30~15:30
Venue: LAROSE 1
Session 1: 8 presentations- Topic: ― Agricultural Science and Food
Engineering‖
Session Chair: Prof. PAUL B. BROWN
B3004 Presentation 1 (13:30~13:45)
Use of Unmanned Aerial Vehicles in Crop Protection
Dr. Piyush Mishra, Vikas KT, Madhu KS, Babu KS, Dr. Mallikarjunappa S., Dr. L
Venkatakrishnan, S Sudhakar and P Suriyanarayanan
Tata Sons Group Technology and Innovation Office, India
Abstract—Despite having one of the largest arable and irrigated land India ranks poorly in
farm productivity due to factors including lack of market- appropriate technologies, use of
non-standard practices, and growing shortage and cost of farm labor. This paper discusses
research and development of UAV-based low-volume spraying techniques, which have been
successfully validated on multiple crops, and presents key results including efficacy,
phytotoxicity, efficiency, and crop-specific optimizations required to achieve industry
benchmarks. Currently, commercial field pilots are underway to validate market-appropriate
business model(s) to offer UAV aerial spraying agri-services.
2018 HKCBEES BANGKOK CONFERENCE
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Afternoon, January 25, 2018 (Thursday)
Time: 13:30~15:30
Venue: LAROSE 1
Session 1: 8 presentations- Topic: ―Agricultural Science and Food
Engineering‖
Session Chair: Prof. PAUL B. BROWN
B0026 Presentation 2 (13:45~14:00)
Economics of Big Data in U.S. Agriculture
Mitchell Morehart
Authoritative Analytics, USA
Abstract—Are the economic benefits to producers and the agricultural sector from Big Data, illusionary
or real? The phenomenon of Big Data in U.S. agriculture encompasses diverse and massive volumes of
data that can be captured, analyzed and used for decision-making by a variety of stakeholders. The
landscape of stakeholders exhibits an interesting intersection between powerful technology companies,
venture capitalists, and often small start-ups and new entrants. The agriculture sector is well placed to
leverage big-data technology and many companies are contributing to its success. Every aspect of crop and
animal production will soon have data capture capabilities. This talk will highlight the historical
development of Big Data in agriculture. It will examine two primary vessels for economic gain;
improvements in supply chain linkages and improvements in on-farm production practices. Supply chain
traceability can reduce exposure to hazardous foods and reduce quality deterioration. Moreover, a more
systematic alignment along the supply chain from input supplier and manufacturing to food retailer has the
potential to increase efficiency through better product flow scheduling and inventory management.
Inefficiencies in planting, harvesting, water use, transportation, as well as uncertainty about the weather,
pests, consumer demand and other intangibles can be greatly reduced by these emerging technologies. The
role of software and how Big Data is being used to provide predictive insights in farming operations, drive
real-time operational decisions, and redesign agricultural production processes will be discussed. Data
privacy and ownership have been topics of discussion in precision agriculture since the first global
positioning system (GPS) began recording data geospatially more than 25 years ago. The line between
what data and information farmers are willing to share and a company is allowed to use will keep shifting.
At the same time, there are several public institutions that publish open data, under the condition that the
privacy of persons must be guaranteed. Clearly, there are no easy solutions. Growers need to be
circumspect in the management and protection of their data and information and be knowledgeable about
the purposes and practices of other individuals and companies with whom they are sharing data and
information.
2018 HKCBEES BANGKOK CONFERENCE
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Afternoon, January 25, 2018 (Thursday)
Time: 13:30~15:30
Venue: LAROSE 1
Session 1: 8 presentations- Topic: ―Biology and Biotechnology‖
Session Chair: Prof. PAUL B. BROWN
S1001 Presentation 3 (14:00~14:15)
Fish Allergenicity Modulation towards the Production of a Low Allergen Farmed Fish; a
Proteomics Approach
Pedro M L Rodrigues, Denise Schrama and Cláudia Raposo
University of Algarve, Portugal
Abstract—Food allergies are a significant public health concern throughout the world. Fish is
a food product increasingly consumed worldwide due to its high nutritional value and healthy
meat. Unfortunately, like many food sources, fish can cause adverse immune-mediate
reactions in some individuals. This allergic reaction to food, affects 1-3% of the population
and about 4-6% of children. The main fish allergen is β-parvalbumin; a small and highly
stable muscle protein. Fish parvalbumins are highly conserved proteins, which are binding
bivalent ions, calcium or magnesium. In fish-allergic patients, specific IgE cross-react mostly
with parvalbumins from different fish, especially when recognizing highly identical protein
regions involved in the ion binding. Other fish allergens have been identified as well - such
as enolases, aldolases or fish gelatin - but their importance has been only shown for a limited
number of fish species. Currently, there is no cure available for fish allergies with the clinical
management of fish allergy solely relying on a strict avoidance diet. Modulation of fish
allergenicity towards the production of a low allergen farmed fish was firstly attempted by
our group. This has been done with specifically designed fish diets, enriched in components
and small changes introduced in the fish farming process that target the expression or
inactivation of the main fish allergen; parvalbumin. Proteomics is the chosen technique to
access fish allergens characterization and expression in muscle while IgE assays to confirm
the lower allergenic potential of this fish are conducted in patients with history of fish
allergies.
2018 HKCBEES BANGKOK CONFERENCE
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Afternoon, January 25, 2018 (Thursday)
Time: 13:30~15:30
Venue: LAROSE 1
Session 1: 8 presentations- Topic: ―Biology and Biotechnology‖
Session Chair: Prof. PAUL B. BROWN
S2001 Presentation 4 (14:15~14:30)
Review of Environmental Sustainability Assessments of Aquaculture Systems: Main Findings
and Outlook
Florence Alexia Bohnes, Michael Zwicky Hauschild, Jørgen Schlundt and Alexis Laurent
Technical University of Denmark, Denmark
Abstract—Aquaculture has been growing quickly during the last decades and, albeit often
regarded as sustainable by nature, this sector remains associated with several environmental
impacts (e.g. aquatic eutrophication, ecotoxicity impacts in local ecosystems). To assess
environmental sustainability of food production systems, one of the most commonly used
tools is life cycle assessment (LCA), which quantifies the impacts of a system along its
whole life cycle. Over the past decades, LCA has been applied to aquaculture systems, but
what are the lessons we learn from them? To address this question, we performed an
unprecedentedly comprehensive critical review encompassing 65 LCA studies of aquaculture
systems published in peer-reviewed journals. We conducted a statistical meta-analysis of the
results, and performed comparative analyses of the fish farms and practices, accounting for
differences in intensity, technology, feed conversion ratio (FCR), and types of farmed
species. Overall, we found that FCR, the species and the technology have a significant
influence on the environmental impacts per produced output. The intensification of the
aquaculture systems is usually associated with environmental burden-shifting from local to
global impacts. Polyculture is a promising candidate for more sustainable aquaculture
systems, especially integrated agriculture-aquaculture and aquaponics. Therefore, in the quest
for more environmental-friendly aquaculture systems, we recommend to (1) focus on
reducing the FCR and choose environmental-friendly diets (e.g. replacing fish meal and fish
oil ingredients by crop ingredients); and (2) implement systems such as closed-systems or
polyculture, that allow efficient management of nutrients without creating burden-shifting
with energy demand.
2018 HKCBEES BANGKOK CONFERENCE
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Afternoon, January 25, 2018 (Thursday)
Time: 13:30~15:30
Venue: LAROSE 1
Session 1: 8 presentations- Topic: ―Biology and Biotechnology‖
Session Chair: Prof. PAUL B. BROWN
S0003 Presentation 5 (14:30~14:45)
Research on the Demand and Availability of Formal Loan in Aquaculture —Taking the
Flatfish Farmers as an Example
Zhang Yingli, Yang Zhengyong, Zhang Di and Shen Xin
College of Economics and Management, Shanghai Ocean University, Shanghai, China
Abstract—Based on the survey data from Shandong and Liaoning Province, this paper
investigates the factors that influencing the formal loan demand and availability of
aquaculture farmers in China. The results show that: (1) Culture area and technical level have
the positive impacts on the formal loan demand. (2)Technical level and the number of bank
visit for loans can have the positive impact on the formal loan availability. But the scale of
loans and the distance to financial institutions have a negative impact on the formal loan
availability. In addition, Liaoning Province loan availability is significantly higher than that
in Shandong Province. (3) Culture area and technical level have positive influence on both
applied and approved loan amounts. (4) The applied and approved loan amounts for factory
culture are both significantly higher than those of pond culture.
2018 HKCBEES BANGKOK CONFERENCE
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Afternoon, January 25, 2018 (Thursday)
Time: 13:30~15:30
Venue: LAROSE 1
Session 1: 8 presentations- Topic: ―Biology and Biotechnology‖
Session Chair: Prof. PAUL B. BROWN
B4001 Presentation 6 (14:45~15:00)
Storage Temperature and Packaging Treatment on Antioxidant Capacity of Ciplukan (Physalis
Peruviana L.)
Aldila Putri Rahayu and Deffi Armita
Brawijaya University, Indonesia
Abstract—Indonesia is a country rich in various local fruits that are not too familiar among
the people, one of them is Ciplukan. Ciplukan or better known as a Cape Goose Berry, is
―super fruit‖ because it contain of many beneficial compunds such as flavonoids, alkaloids,
terpen, ascorbic acid and karetonoid. Carotene and lycopene are compounds that are easily
affected by temperature. The objective this study is to determine the effect of Ciplukan
storage treatment on the content of beta carotene and lycopene. The treatments were two
storage temperature (4°C and 10°C [room temperature]) and packaging (package and
un-package). The fruit with room temperature and packaging in plastic storage, showed
significantly higher beta carotene and lycopene content than fruit that storage in 4°C and
without packaging.
2018 HKCBEES BANGKOK CONFERENCE
- 27 -
Afternoon, January 25, 2018 (Thursday)
Time: 13:30~15:30
Venue: LAROSE 1
Session 1: 8 presentations- Topic: ―Biology and Biotechnology‖
Session Chair: Prof. PAUL B. BROWN
B0020 Presentation 7 (15:00~15:15)
Early Feasibility Study on Capparis Production and Processing in Hamedan Province in Iran
Mahdi Reyahi-Khoram and Reihaneh Reyahi-Khoram
Research and Development Expert in Sahar Food Industries Company, Hamedan, Iran
Abstract—Capparis spinosa is one of the most widely consumed aromatic and medicinal
plants in Iran whose local name is ―shapaleh‖. The aim of the present study was to evaluate
whether the production and processing of Capparis in Asadabad Township in Hamedan
province is suitable and sustainable. The food processing industry is one of the basic
industries operating in Iran and is divided into several sections. Iran is the second largest
economy in the Middle East and North Africa, Iran annually produces 12 million tones of
fresh fruits and 23 million tons vegetables. In this situation, it is necessary to select kind of
crop, technology tools and apply methods to improve them. Today in Iran, there are three
industrial companies which are engaged in industrial processing of Capparis. One of the
mentioned industries is situated in Hamedan province with name ―Sahar Food Industries
Company‖. It is concluded that, if local authorities are honest about their needs, Stakeholders
will be able to give feedback, receive information and be informed of major decisions made.
2018 HKCBEES BANGKOK CONFERENCE
- 28 -
Afternoon, January 25, 2018 (Thursday)
Time: 13:30~15:30
Venue: LAROSE 1
Session 1: 8 presentations- Topic: ―Biology and Biotechnology‖
Session Chair: Prof. PAUL B. BROWN
B0043 Presentation 8 (15:15~15:30)
Optimization of Ultrasonic Assisted Extraction (UAE) of Anthocyanins from Black Glutinous
Rice and Evaluation of their Antioxidant Properties
Anjali Maniyam Pariyarath, Anil. K. Anal and Muhammad Bilal Sadiq
Asian Institute of Technology, Thailand
Abstract—In the present study, the green technological approach – ultrasonic assisted
extraction (UAE) was employed for the extraction of anthocyanins, total phenolic compounds
(TPC) and their antioxidant activities from black glutinous rice. The effects of ethanol
concentration, amplitude and time were investigated using Box – Behnken design and
extraction conditions were optimized by using response surface methodology (RSM). The
optimal ethanol concentration, extraction time and amplitude were 65%, 15 min and 100%
respectively. The maximum anthocyanin content, TPC and antioxidant activity were found as
47.45 mg cyn-3-glucosidase/gram fresh weight ml, 83.07mg GAE/ g, 99.59 % for DPPH and
0.19 mg AAE/g. Statistically, only concentration of ethanol indicated significant effect on the
extraction. The ethanol concentration of 65% gave high yield of anthocyanins, TPC and
antioxidant activities. In the case of amplitude and time, there was no significant difference
compared to ethanol concentration. This study to a certain extent revealed important bioactive
properties of black glutinous rice. i.e, the high antioxidant activity, presence of anthocyanins
and TPC.
16:15~16:30 Coffee Break
2018 HKCBEES BANGKOK CONFERENCE
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Session 2
Tips: The schedule for each presentation is for reference only. In order not to miss your presentation,
we strongly suggest that you attend the whole session.
Afternoon, January 25, 2018 (Thursday)
Time: 13:30~14:45
Venue: LAROSE 2
Session 2: 5 presentations- Topic: ―Biology and Biotechnology‖
Session Chair: Prof. Hans-Uwe Dahms
B0037 Presentation 1 (13:30~13:45)
Microcrustacean Moina macrocopa: An Important Feed for Aquaculture and a Potential
Bioindicator for Heavy Metals Contamination in Aquatic Environments
Chanya Phromchaloem, Amonrujee Nakphlaiphan and Thanit Pewnim
Department of Chemistry, Faculty of Science, Silpakorn University, Thailand
Abstract—Water flea Moina macrocopa is a microcrustacean found in abundant in tropical
countries such as Thailand. The micro crustacean has been used extensively in rearing fish
fingerlings, both ornamental fishes and edible fishes. While the aquaculture industry of
Siamese fighting fish (Betta splendens) relies heavily on the cultivation and use of Moina
macrocopa, practically as the only feed for the fish, the raising of fresh water fish such as
catfish and tilapia use Moina macrocopa to feed the fish fingerlings until they reach a size
that can feed on pellets. This paper surveys the production of the microcrustacean and its
successful use in aquaculture in Thailand, while at the same time investigating the potential
use of this microcrustacean as a bioindicator for heavy metals contamination. Laboratory
investigation found that treating Moina macrocopa with cadmium at a concentration of 160
µg L-1
for 48 h resulted in cell nuclei damages as judged by single cell gel electrophoresis or
the Comet Assay. It was also found through gel filtration experiment and the SDS-PAGE
analysis that cadmium induced the synthesis of a cadmium binding protein having a
molecular mass around 50-55 kDa. A comparative study on cadmium toxicity was also
carried out with Drosophila melanogaster larvae. It was found that the heavy metal induced
the synthesis of cysteine-rich proteins in the larvae of the insect. It can be concluded that
while Moina macrocopa is useful as a live feed in aquaculture, the microcrustacean has a
potential for use as a bioindicator for heavy metals contamination in aquatic environments.
2018 HKCBEES BANGKOK CONFERENCE
- 30 -
Afternoon, January 25, 2018 (Thursday)
Time: 13:30~14:45
Venue: LAROSE 2
Session 2: 5 presentations- Topic: ―Biology and Biotechnology‖
Session Chair: Prof. Hans-Uwe Dahms
B0009 Presentation 2 (13:45~14:00)
Trehalose Association for Drought Tolerance in Wheat (Triticum aestivum L.) Germplasm
through SSR Markers
Muhammad J. Iqbal, Naureen Shams and Amer Jamil
COMSATS Institute of Information Technology, Defence Road, Lahore, Pakistan.
Abstract—Drought, one of the abiotic stresses, substantially hampers the crop productivities
worldwide. For mitigating the stress, in plant cell various biochemical mechanisms including
the synthesis of trehalose which is a known intrinsic response of drought tolerant plant
species. Cultivated hexaploid wheat (Triticum aestivum L.) is one of the most important
staple food crops in many countries including India and Pakistan. It often experiences
drought stress, and would be much more devastating under the upcoming new challenge of
climate change. In the present study, a total of 66 diverse wheat genotypes including cultivars
were grown under well watered and limited water conditions. DNA fingerprinting of wheat
germplasm of the selected genotypes was done on the basis of polymorphism using 45 SSR
primer pairs and tested for polymorphism among different genotypes viz Local Genotype,
Mapping Population, Synthetic and NIBGE. The dendrogram results have shown the
genotype association with the levels of trehalose during induced drought stress. The
relationship between pattern of drought responsive biochemical attributes and DNA markers
in the selected wheat genotypes was established with a recommendation to select drought
tolerant genotypes for sowing in drought affected areas.
2018 HKCBEES BANGKOK CONFERENCE
- 31 -
Afternoon, January 25, 2018 (Thursday)
Time: 13:30~14:45
Venue: LAROSE 2
Session 2: 5 presentations- Topic: ―Biology and Biotechnology‖
Session Chair: Prof. Hans-Uwe Dahms
B0038 Presentation 3 (14:00~14:15)
Effect of Plant Growth Promoting Rhizobacteria on Growth and Yield of 2 Red Lettuce
Cultivars
Wisnu Eko Murdiono, Koesriharti and Palupi Wulandari
Brawijaya University, Indonesia
Abstract—Red Lettuce (Lactuca sativa) cultivars, such as Red Rapid and Lollo Rossa, play
an important factor in order to increase Indonesia's vegetable production to meet the domestic
demand. The use of biological fertilizers, such as Plant Growth Promoting Rhizobacteria
(PGPR) are another important factor to increase production in sustained agricultural systems.
The purpose of this experiment was to study the effect of PGPR applied on the growth and
yield of two red lettuce cultivars. This experiment was conducted in Dadaprejo Village,
Junrejo District, Batu in July - August 2017. This was a factorial experiment arranged in
Completely Randomized Block Design which consisted of 2 factors. The first factor was two
red lettuce cultivars consisted of Red Rapid (V1) and Lollo Rossa (V2). The second factor
was a PGPR dosage consisted of 6 levels : without PGPR (P0), 100 ml PGPR (P1), 150 ml
PGPR (P2), 200 ml PGPR (P3), 250 ml PGPR (P4) and 300 ml PGPR (P5 ). The observed
growth parameters were plant height and number of leaves at 10, 20, 30 and 40 days after
transplanting. Observations made during harvest were root length and weight, total fresh
weight and also consumable weight. The result showed that there were interactions between
PGPR application and red lettuce cultivars on all harvest parameters. On both red lettuce
cultivars observed, application of 200 ml PGPR affected on higher root length and weight,
while applying 300 ml of PGPR produced higher consumable weight and highest total fresh
weight than any other dosage. Each PGPR dosage combined with Red Rapid produced higher
root and total fresh weight than Lollo Rossa.
2018 HKCBEES BANGKOK CONFERENCE
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Afternoon, January 25, 2018 (Thursday)
Time: 13:30~14:45
Venue: LAROSE 2
Session 2: 5 presentations- Topic: ―Biology and Biotechnology‖
Session Chair: Prof. Hans-Uwe Dahms
B0022 Presentation 4 (14:15~14:30)
Telemetric Lactic Acid Monitoring System for Real-time Assessment of Exercise Intensity
Keigo Enomoto, Takahiro Kurose and Hiroyuki Kudo
Meiji University Graduate School, Japan
Abstract—A telemetric lactic acid(LA) monitoring system was developed and telemetry of
sweat LA of jogging subject was conducted for the first time. Non-invasive assessment of
blood LA is strongly requested in a wide variety offields such as conditioning and training in
sports, care in medical facilities and triage at disaster site. Previously, we reported a
microfluidic bio-sensing system for continuous LA monitoring. Although the performance
was satisfactory, it was still difficult to utilize the system to the subject freely moves around
because of large equipment (syringe pumps, electrochemical analyzer and sensing element) as
well as presence of tubes for liquid supply and electric wiring. In this study, (1)a micro
fabricated biosensor with lactate oxidase-modified platinum electrode, (2) piezoelectric micro
pumps (25mm x 25mm x 4.8mm)and (3)miniaturized potentiostat which is capable of
Bluetooth interface were newly employed for the purpose of constructing a portable LA
monitoring system. Our system measures sweat LA by attaching a flexible sampling device
with a micro flow channel(1 mm x 30 mm x 0.2 mm) on the surface of the skin and
transporting whole secretions to the biosensor using a continuous career flow of phosphate
buffer saline. The biosensor measures LA as production of hydrogen peroxide produced in
enzymatic conversion of LA to pyruvic acid. Sufficient sensitivity (LA :53.14 nA・mM-1)
and response time(t95:8.3s)was obtained. In order to keep sufficient suction pressure even if
the position or angle of the system changed due to exercise, two piezoelectric micro
pumps(25mm x 25mm x 4.8mm)were connected in series. This enabled sweat lactate
monitoring under the conditions involving free movement.
Under the approval of ethical committee of Meiji University, the LA monitoring system was
utilized in wireless sweat LA monitoring under the different sweating conditions (sweating by
rising ambient temperature and sweating by jogging).As a result, secretion of lactic acid from
the subject moving around was successfully measured continuously and increased by 3.6
folds after 20 minutes jogging workout. Temporal changes of sweat LA correlated with the
kinetics of blood LA rather than sweat rate in both cases. Further characteristics of the
wireless LA monitoring system, results of sweat LA monitoring and possible applications will
be presented in detail at the conference.
2018 HKCBEES BANGKOK CONFERENCE
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Afternoon, January 25, 2018 (Thursday)
Time: 13:30~14:45
Venue: LAROSE 2
Session 2: 5 presentations- Topic: ―Biology and Biotechnology‖
Session Chair: Prof. Hans-Uwe Dahms
B0013 Presentation 5 (14:30~14:45)
Enhances Mitochondrial Function in Human Neuronal SH-SY-5Y Cell Line via 3D Bioprinter
Ming-Chang Chiang
Department of Life Science, College of Science and Engineering, Fu Jen Catholic University,
Taiwan
Abstract—Direct-write printing of cells within bioinks presents an opportunity for in vitro
modeling and regenerative medicine. The structure and function of the human nervous system
are altered in 3D when compared with 2D. To investigate directly the influence of 3D
conditions which may be beneficial for cultivation and proliferation of human neural
SH-SY-5Y cell line, the 3D Bioprinter developed at the CELLINK INKREDIBLE was used.
3D Bioprinter allows the creation of a unique environment of low shear force, high-mass
transfer and enables 3) cell culture of dissimilar cell types. The results show that 3D
conditions using an 3D Bioprinter would induce the expression of intracellular mitochondrial
genes, including PGC1(PPAR coactivator 1), nuclear respiratory factors 1 and 2 (NRF1 and
NRF2) and mitochondrial transcription factor A (Tfam) were all significantly increased at 5
day after the onset of 3D. Accordingly and importantly, the ATP level and amount of
mitochondrial mass were also increased. These results suggest that exposure to 3D conditions
using an 3D Bioprinter would increase mitochondrial function. In addition, the 3D Bioprinter
would support cells growth, which may have the potential for cell replacement therapy in
neurological disorders. The 3D conditions will promote investigation of human neuronal
development, function, and disease, and may be adjustable for engineering 3D brain.
2018 HKCBEES BANGKOK CONFERENCE
- 34 -
Session 3
Tips: The schedule for each presentation is for reference only. In order not to miss your presentation,
we strongly suggest that you attend the whole session.
Afternoon, January 25, 2018 (Thursday)
Time: 14:45~16:15
Venue: LAROSE 2
Session 3: 6 presentations- Topic: ―Environmental and Chemical
Engineering‖
Session Chair: Prof. Ching Chang Lee
S0006 Presentation 1 (14:45~15:00)
Application of Remote Sensing and GIS for Water Environment Suitability Evaluation
Around Lampung Bay and Hurun Bay, Indonesia
Andiyanti Putri Estigade, Ariani Puji Astuti, Arief Wicaksono, Dea Nadia, Mousafi Dimas
Afrizal, Muhammad Hilmy Aziz, Tika Maitela
Universitas Gadjah Mada, Indonesia
Abstract—Water environment suitability evaluation is a prominent requirement to determine
the success rate of sustainable aquaculture business in coastal waters. This study aims to
evaluate the site of floating net cages which already exist in Lampung Bay using water
environment site suitability maps and interview data. Physical water parameter suitability
maps for floating net cage cultivation are generated through spatial modeling of depth, sea
surface temperature, water clarity, suspended solids, salinity, chlorophyll-α, and pH
parameters. All data are combined by using weighted overlay in GIS environment. The result
shows that the development of floating net cages around Sariringgung Beach has good
suitability level whereas Lampung Bay do not meet the suitable class criteria of depth, surface
temperature, and clarity parameters. Disparity on land cover conditions and the understanding
of floating net cage cultivation among the farmers on both sites are the factors that make those
different level of suitability classes.
2018 HKCBEES BANGKOK CONFERENCE
- 35 -
Afternoon, January 25, 2018 (Thursday)
Time: 14:45~16:15
Venue: LAROSE 2
Session 3: 6 presentations- Topic: ―Environmental and Chemical
Engineering‖
Session Chair: Prof. Ching Chang Lee
B0010 Presentation 2 (15:00~15:15)
Exploring a New Way to Create Pectin Hydrogels
Havazelet Bianco-Peled, Irit Ventura and Lena Neufeld
Technion Israel Institute of Technology, Israel
Abstract—Pectin is a complex group of food heteropolysaccharides found mainly in primary
cell walls and middle lamella of most plants. Commercially, pectin is mostly produced from
citrus peel or apple pomace, both by-products of juice production. Pectin is widely used in
the food industry as a texturizer or stabilizer. In addition, applications in the area of
pharmaceutical and biomedical engineering have recently emerged.
Many functional attributes of pectin are based on its ability to form gels. Traditionally, high
methylester (HM) pectin gels are fabricated in the presence of sugars and an acidic
environment, whereas low methylester (LM) pectin gels are prepared in the presence of
divalent ions such as calcium.
It has been recently discovered that hot solutions containing pectin and chitosan demonstrate
gelation occurring upon cooling to room temperature. In our study we characterize the
resulting hydrogels in term of swelling, mechanical properties, viscosity and rheology. Further,
we explore the origin of this gelation using small angle X-ray scattering (SAXS). Modeling
SAXS patterns revealed rigid rod conformation in chitosan solutions, whereas pectin formed a
self-avoiding chain. In addition, nanometric aggregates were detected in pectin solutions.
Pectin and chitosan in mixed solutions adopted a similar semiflexible conformation. The only
sample that created a visually stable gel displayed a different pattern, characteristic to
polymers gels. Manipulating the composition by adding urea and salts highlighted the
important role of hydrogen bonding, which governs the gelation mechanism.
2018 HKCBEES BANGKOK CONFERENCE
- 36 -
Afternoon, January 25, 2018 (Thursday)
Time: 14:45~16:15
Venue: LAROSE 2
Session 3: 6 presentations- Topic: ―Environmental and Chemical
Engineering‖
Session Chair: Prof. Ching Chang Lee
B0029 Presentation 3 (15:15~15:30)
Thick Activated Carbon Sheet Electrode and Hydrophobic Organic Matter Improve the
Performance of Microbial Fuel Cell
Naoki Hayashi, Dang Trang Nguyen and Kozo Taguchi
Ritsumeikan University, Japan
Abstract—Recently, there are a lot of environmental problems all over the world.
Mass-consumption of fossil fuels contribute to depletion of them, emission of large amount of
carbon dioxide, and global warming. However, fossil fuels are used everywhere and they are
essential for modern life to get energy. A microbial fuel cell (MFC) is one of the new energy
resources. This study focused on activated carbon sheet that has property of absorbing organic
matter and large surface area. In this study, the experiment was conducted in eight conditions
that were combined organic matter, kind of electrode and thickness of the electrode. Biofilm
was built on two kinds of anodes that were made of carbon sheet and activated carbon sheet
by using glucose or malt powder. The combination of using activated carbon sheet and malt
powder produced maximum current density of 220 μA/cm2.
2018 HKCBEES BANGKOK CONFERENCE
- 37 -
Afternoon, January 25, 2018 (Thursday)
Time: 14:45~16:15
Venue: LAROSE 2
Session 3: 6 presentations- Topic: ―Environmental and Chemical
Engineering‖
Session Chair: Prof. Ching Chang Lee
B0027 Presentation 4 (15:30~15:45)
Optimum Conditions for Titanium Oxide Thin Film on Dye-Sensitized Solar Cells Using
Organic Dye Sensitizer-MK2
Yoshiki Kurokawa, Dang Trang Nguyen and Kozo Taguchi
Ritsumeikan University, Japan
Abstract—In this paper, we propose fabricating method of high quality titanium oxide ( )
thin film for Dye-Sensitized Solar Cell (DSSC) by Electrophoresis Deposition (EPD). We
focused on stirring time of solution, multilayer thin film and addition of EPD
solution. Stirring time of solution was conducted for 1, 3, 6, 12, 24, 48 hours, and
multilayer thin film was fabricated three types (single, double, four layer). Addition of EPD
solution was adopted polyethylene glycol (PEG). The amount of PEG was conducted five
types (0, 5, 15, 35 and 50 g/L).MK2 dye which is organic dye was used as photosensitizer. As
a result, the highest conversion efficiency was obtained at 4.12% with stirring for 24 hours,
four layers and 5g/L PEG.
2018 HKCBEES BANGKOK CONFERENCE
- 38 -
Afternoon, January 25, 2018 (Thursday)
Time: 14:45~16:15
Venue: LAROSE 2
Session 3: 6 presentations- Topic: ―Environmental and Chemical
Engineering‖
Session Chair: Prof. Ching Chang Lee
B0039 Presentation 5 (15:45~16:00)
Impact of Complimentary/Restricted Irrigation on Wheat Yield and Its Components
Shahryar Sasani, Rahman Rajabi and Mosayeb Novroozi
Crop and Horticultural Sciences Research Department, KANRREC, AREEO, Iran
Abstract—Iran country has located on arid/semiarid belt of globe, and because of climate change
impacts, drought has been occurred frequently during last two decades in this country particularly at
late growing season on cereal fields. Consequently, wheat cultivars with less water requirement in
spring and high efficiency with acceptable grain yield are cardinal points on wheat breeding programs
in such an arid part of world. Eight recent-released bread-wheat cultivars: (Rijaw, Karim, Parsi,
Sivand, Pishgam, Sirvan, Oroom & Zaree) along with three superior bread wheat lines (Cross of
Sabalan, Cross of Alborz & DN-11) were compared with two local check cultivars (Sardari & Pishtaz).
Data were analyzed as a Combined Analysis experiment at four trial areas, based on Randomized
Complete Block Design (RCBD) with three replicates on two Agricultural Research Stations
(Islamabad and Sararood) at two continues cropping cycles in Kermanshah province of Iran. Trial
areas were run on: rainfed condition; two times complementary irrigation at grain filling stage on
rainfed situation; terminal drought stress as irrigation cut treatment regime done from boot stage and
continues to physiological maturity stage, and finally, full irrigation situation as control. Based on
two years trial combined results, two complementary irrigation applications (average grain yield was
2541 kg/ha) caused a significant increase on grain yield of considered genotypes (+115%) in general,
compared to rainfed condition (average grain yield was 1182 kg/ha); best response among genotypes
to couple complimentary irrigation was belonging to Sivand cv. and Pishgam cv. by three time
increases in grain yield. Terminal drought stress - with 3861 kg/ha as average grain yield - caused to
40% decrease on grain yield in general in comparison to full irrigation treatment (average grain yield
was 6482 kg/ha), although, Sivand, Parsi and Karim lost about 50% of their grain yield under terminal
drought stress. Terminal drought stress condition was better than double complimentary irrigation
because of 52% more production on grain yield. As the summarized results: Sirvan cv. produced the
most grain yield at normal condition; Pishgam cv. produced the most grain yield at terminal drought
stress situation; Sivand cv. produced the most grain yield at complementary irrigation case, and finally,
Zaree cv. produced the most grain yield at rainfed site in comparison with other genotypes.
2018 HKCBEES BANGKOK CONFERENCE
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Afternoon, January 25, 2018 (Thursday)
Time: 14:45~16:15
Venue: LAROSE 2
Session 3: 6 presentations- Topic: ―Environmental and Chemical
Engineering‖
Session Chair: Prof. Ching Chang Lee
B4003 Presentation 6 (16:00~16:15)
Contamination of Mercury around the Artisanal and Small-Scale Gold Mining Area and the
Potential of Sisal (Agave sisalana) for Phytoremediation of the Mercury Contaminated Soil
Sachiko Takahi 1and Kokyo Oh
2
1. Faculty of Education and Human Studies, Akita University, Japan
2. Center for Environmental Science in Saitama, Japan
Abstract—Indonesia is a major location for artisanal and small-scale gold mining (ASGM)
activities in which large amount of mercury is used to extract gold from ore and the mercury
used is then released to environment. In this study, the mercury in the soils, tailings, sediment,
and waste water was investigated, and the potential of sisal (Agave sisalana) as an economic
fiber plant for phytoremediation of the mercury contaminated soil was estimated around the
ASGM area in West Sumbawa, West Nusa Tenggara, Indonesia. The results showed that the
average concentration of total mercury in the yard soil of ASGM was 41.1 mg kg-1 (about 3
times of the environmental threshold limits of Japan), ranged from 0.25 to 226 mg kg-1. Over
60% of the sampling sites exceeded the environmental threshold limit of Japan. Sisal had
average mercury concentrations of 2.1 mg kg-1 in the leaves, and 14.7 mg kg-1 in roots. It
was estimated that sisal could had an annual phytoremediation potential of mercury ranged
from 21,00 to 880,000 mg ha-1. This study suggested sisal could be a suitable economic fiber
crop for soil contamination of mercury.(This work was supported by JSPS KAKENHI No.
2530100, JSPS-Bilateral Programs Joint Research Projects- 2015-2017FY, and Center for
Southeast Asian Studies, Kyoto University,-International Program of Collaborative Research).
2018 HKCBEES BANGKOK CONFERENCE
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Poster Session January 25, 2018 (Thursday)
Time: 16:30~18:30
Venue: LAROSE 1
Poster Session: 16 posters
Session Chair: Assoc. Prof. Win Chaeychomsri
S1002 Poster 1 (16:30~18:30)
Effects of Histidine, Threonine and Tryptophan Supplementation on the Performance of
Gilthead Seabream Using 2D-DIGE.
Denise Schrama, Rita Colen, Sofia Engrola, Tomé Silva, Pedro M.L. Rodrigues
University of Algarve, Portugal
Abstract—Human fish consumption is continuously growing and aquaculture production
costs need to be minimized, while keeping optimal growth and fish health. Plant-based
protein ingredients are an alternative for fish diets but may influence growth and stress due to
imbalanced amino acids profiles. Evaluation of the functional effect of diet supplementation
with three amino acids, like histidine: involved in biochemical changes which occur during
inflammation; threonine: involved in the immune system response, and tryptophan: involved
in stress and inflammatory responses will be performed using differential proteomics.
Juvenile gilthead seabream were fed with three diets, which were formulated based on
estimated requirements. Diet 1 had 0% of the three amino acids (histidine, threonine and
tryptophan), diet 2 had 1.1% (w/w) of supplementation and diet 3 was supplemented with
1.5% (w/w). Fish were fed twice a day, ad libitum and maintained in natural flow-through
seawater and artificial aeration (dissolved oxygen above 5 mg L-1). After 2 weeks fish were
lethally anesthetized with 2-phenoxyethanol, liver was collected and kept at -80ºC for further
analyses. Proteins were extracted using a DIGE buffer and quantified by the Bradford
method. Proteins were separated using 2D-DIGE on 24 cm drystrips with pH 4-7. Gels were
analyzed using SameSpots software and 19 spots showed significant difference after
statistical analysis (ANOVA, p<0.05) between the diets. Differentially expressed proteins
were sequenced using mass spectrometry. Identified proteins were related to protein transport
– namely transportin-2 and cell signaling – namely mucin 13. These proteins were both
upregulated in diet 2 (1.1% supplementation with histidine, threonine and tryptophan),
showing that these amino acids might influence the functional state of gilthead seabream.
2018 HKCBEES BANGKOK CONFERENCE
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January 25, 2018 (Thursday)
Time: 16:30~18:30
Venue: LAROSE 1
Poster Session: 16 posters
Session Chair: Assoc. Prof. Win Chaeychomsri
S4001 Poster 2 (16:30~18:30)
Atypical Furunculosis Caused by Aeromonas salmonicida subsp. masoucida Causes Mortality
in Farmed Korean Rockfish (Sebastes schlegelii)
Do-Hyung Kim and Chan-Il Park
College of Fisheries Science, Pukyong National University, Republic of Korea
Abstract—Atypical Aeromonas salmonicida (RFAS1, 2, 3) were first isolated from diseased
rockfish in fish farms, Geomundo. This slow growing and non-pigmented pathogen caused
mortalities in rockfish farms from December to May. Typical symptoms of the diseased fish
were ulcer lesions over body surface, hamorrhages in gills, eyes and intestine, and swollen
spleen and kidney. The isolates were used for exmination of phenotypic and genotypic
characteristics, challenge test, and survival ability in rockfish serum. A reference strain, A.
salmonicida subsp. masoucida (KCCM 40239), originally isolated from rainbow trout, was
used for comparison. Biochemical characteristics of the isolates and the reference strain were
very similar with some different profiles, such as fermentation of saccharose and amygdalin.
LD50 values of RFAS1 strain were 1.5ⅹ 105.25 and 1.5 ⅹ 106.4 cfu fish-1, challenged by
i.p. and i.m., respectively. Five house-keeping genes (16S rRNA, gyrB, rpoD, dnaJ and recA)
of RFAS1-3 showed the 3 isolates were most closely related to A. salmonicida, but could not
be identified at a subspecies level. According to analysis of vapA gene, encoding A-layer
protein, RFAS 1-3 strains were most closely related to A. salmonicida subsp. masoucida.
However, only RFAS1-3 showed hemolytic activity against rockfish erythrocytes and were
able to survive in rockfish serum but cells of KCCM 40239 decreased rapidly and killed after
12 hours of incubation in the serum. Therefore, newly isolated atypical A. salmonicida are
pathogenic to rockfish, which is the first case of the species isolation from marine fish in
Korea.
2018 HKCBEES BANGKOK CONFERENCE
- 42 -
January 25, 2018 (Thursday)
Time: 16:30~18:30
Venue: LAROSE 1
Poster Session: 16 posters
Session Chair: Assoc. Prof. Win Chaeychomsri
S4002 Poster 3 (16:30~18:30)
Distribution of CD83+ Cell of Olive Flounder (Paralichthys olivaceus): Gene Expression
after Viral Haemorrhagic Septicaemia Virus (VHSV) induced Infection in Fish
Do-Hyung Kim and Chan-Il Park
College of Marine Science, Gyeongsang National University, Republic of Korea
Abstract—In 1999, VHSV was first isolated from free-living Japanese flounder. Since then,
which has become one of the most serious diseases among farmed fish species. After viral
infection, dendritic cells (DC) drive the activation and differentiation of T-cell to acquire
effector functions. DCs are sentinel immune cells that play a central role for pathogen
invasion including viral and bacterial infection. Only on DC can show stable surface
expression of CD83 and that gene was describe well as maker of mature DC. We identified
olive flounder CD83 and polyclonal antibody was developed against this gene. Further
investigation, expression analysis after viral infection and DC distribution analysis were
carry out on peripheral blood leukocytes (PBLs) and tissue of olive flounder. OfCD83 gene
up-regulated in liver, spleen, head kidney and intestine after VHSV infection. OfCD83+
signal detected in liver sinusoid, follicular dendritic cells of spleen, kidney, lamina propria of
intestine and second lamellae of gill in healthy olive flounder. Our findings contribute to
understanding the teleost DC mediated immune response.
2018 HKCBEES BANGKOK CONFERENCE
- 43 -
January 25, 2018 (Thursday)
Time: 16:30~18:30
Venue: LAROSE 1
Poster Session: 16 posters
Session Chair: Assoc. Prof. Win Chaeychomsri
B0008 Poster 4 (16:30~18:30)
Synchrotron Radiation for the 3D Anatomical Structure Analysis of Bio-sample
ONSEOK LEE
Soon Chun Hyang University, Korea
Abstract—In this study, we aim to obtain the fundamental data needed for the objective
analysis and bio-mimetics of leeches, using x-ray imaging with radiation that allows 3D
analysis of the detailed anatomic structures of leeches. It was confirmed that through using
radiation, detailed images of leeches can be obtained, allowing 3D analysis of leeches‘
anatomical structures. Also, since the data obtained through the radiation allows you to
observe the micro-structure, it can serve as a good resource for component analysis, as well
as physiological and functional research. More, it is hoped to contribute to further research in
the areas of bio-mimetics using leeches.
2018 HKCBEES BANGKOK CONFERENCE
- 44 -
January 25, 2018 (Thursday)
Time: 16:30~18:30
Venue: LAROSE 1
Poster Session: 16 posters
Session Chair: Assoc. Prof. Win Chaeychomsri
B1005 Poster 5 (16:30~18:30)
Numerical Analysis according to Evaporator Type
Dae Yeong Kim, Moon Ki Kim and Hong Sun Yun
Research and Development Institute, Daesung-Maref CO. LTD., Korea
Abstract—An important part of the refrigeration system used in agricultural product stroage is
evaporators. It is an important device to determine the quality of agricultural products
according to evaporator performance.
Through numerical analysis of the evaporator, it is analyzed that the performance varies with
the type of evaporator. Numerical analysis of the evaporator was performed using the EES
program. The verification of the calculation was verified by comparing with the performance
of the product. The numerical analysis of the evaporator capacity was use to calculate the
evaporator capacity by using the heat transfer coefficient of refrigerant, tube wall, and air. It
compared the numerical analysis of the evaporator capacity and the five my company
products, and showed about 10% error rates. The tendency was analysed according to fins
space, mass flow, heat exchange coil length and air flow. The fin space was changed from
1mm to 20mm in increments of 0.5mm. When the fins are narrow, the air flow is reduced to
lower heat exchange efficiency, and vice versa, resulting in decreased air flow compared to
the refrigerant flow rate, thereby reducing the efficiency of the heat exchanger. The length of
the heat exchanger changed from 500mm to 2400mm in increments of 50mm. As the length
of the heat exchanger increases, the piping friction and the refrigerant temperature rise.
Consequently, the difference in temperature between the refrigerant and the air temperature
decreases, thereby reducing the efficiency of the heat exchanger. The air flow of the changed
from 20CMM to 400CMM in increments of 10CMM. When the air flow rate is higher than a
certain level, the air flow velocity is increased and the heat exchanger efficiency tends to be
lowered. The refrigerant mass flow rate was changed from 3g/sec to 174g/sec in increments of
4.5g/sec. As the flow rate of the refrigerant mass increases it tends to increase proportionately.
However, refrigerant mass flow rate above a certain level, the refrigerant mass flow rate is
larger than the air flow rate, thereby lowering the heat exchange efficiency.
In conclusion, the optimum design of the evaporator depends on the size, load, the evaporator
must be designed in consideration of the fin spacing, heat exchanger coil length, air flow, and
refrigerant mass flow rate.
2018 HKCBEES BANGKOK CONFERENCE
- 45 -
January 25, 2018 (Thursday)
Time: 16:30~18:30
Venue: LAROSE 1
Poster Session: 16 posters
Session Chair: Assoc. Prof. Win Chaeychomsri
B0014 Poster 6 (16:30~18:30)
Phytase Production by Grifola Frondosa and Its Application in Inositol-enriched Solid-state
Fermentation brown Rice
Shih-Jeng Huang, Chieh-Han Chen, Zih-You Chen, Zhi-Wei Lin and Shu-Yao Tsai
Department of Food Nutrition and Health Biotechnology, Asia University, Taichung, Taiwan,
ROC
Abstract—Mushrooms can produce a variety of enzymes and therefore have many
biotransformation capacities. Phytic acid is a ubiquitous anti-nutritional factor in many plant
foods, which results in a lack phytase for monogastric animals (humans, pigs, and chickens)
reducing digestion and absorption of metal elements, protein, starch and lipid. Phytase is
used commercially to maximize phytic acid degradation and to increase inositol in animals.
We analyzed the phytase activity based on 24 kinds of mushroom fruiting bodies. The results
showed G. frondosa has higher phytase activity (1.85 µmol/min of tissue). In liquid-state
fermentation, G. frondosa has high phytase activity of 2.46 µmol/min at 12th grown day. The
increase of inositol content in G. frondosa solid-state fermentation brown rice was 176-fold
as compared to that in the un-fermentation controls. The inositol-enriched G.
frondosa-fermented brown rice demonstrated greater antioxidant properties and contained the
highest antioxidant components than un-fermented brown rice.
2018 HKCBEES BANGKOK CONFERENCE
- 46 -
January 25, 2018 (Thursday)
Time: 16:30~18:30
Venue: LAROSE 1
Poster Session: 16 posters
Session Chair: Assoc. Prof. Win Chaeychomsri
B0016 Poster 7 (16:30~18:30)
Effect of Lateral Displacement of the Papillary Muscles on Functional Mitral Regurgitation
Woojae Hong, Soohwan Jeong and Hyunggun Kim
Sungkyunkwan University, Suwon, Republic of Korea
Abstract—BACKGROUND: Functional mitral regurgitation (FMR) develops when regional or global
enlargement of the left ventricle occurs, which is frequently associated with anomalous displacement
of the papillary muscles (PM). Although studies have identified the mechanisms of abnormal mitral
valve (MV) leaflet tenting leading to MR, the relationship between PM displacement following left
ventricular chamber dilatation and the severity of FMR still remains unclear. In this study, we
investigated the pathophysiologic mechanism of FMR from a biomechanical perspective utilizing our
computational MV evaluation protocol.
METHODS: A computational MV model having no pathologic characteristics at end diastole was
created using our previously developed virtual MV modeling protocol. Three different types of PM
displacement to mimic the pathology of FMR were simulated by altering the normal PM locations
based on previous clinical studies on measurement of PM displacement. The first pathologic MV
model was subjected to PM displacement of 8 mm toward the lateral direction. The second pathologic
MV model was designed with apical PM displacement of 1 mm. For the third pathologic MV model,
both lateral and apical PM displacements were incorporated. A physiologic transvalvular pressure
gradient across the complete cardiac cycle was applied to the ventricular side of the mitral leaflets in
each MV model. Computational dynamic simulations of MV function were performed for these MV
models and compared one another.
RESULTS: The normal MV clearly demonstrated full leaflet coaptation at peak systole. The
pathologic MV having lateral PM displacement revealed abnormal radial elongation of the P2 scallop,
reduced leaflet coaptation, and increased leaflet stress distribution in the P2 scallop. There was little
difference in leaflet coaptation and leaflet stress distribution between the normal MV and the MV with
apical PM displacement. When the PM locations were displaced to both lateral and apical directions,
an excessive stretching of the leaflets was associated with markedly reduced leaflet coaptation and
large stress distribution in the P2 scallop.
CONCLUSIONS: In this study, we demonstrated the effect of lateral and apical PM displacement on
the occurrence and severity of FMR. When both lateral and apical PM displacements occurred, a
substantial lack of leaflet coaptation was observed. Simulation-based biomechanical evaluation of
MV pathology related to left ventricular chamber dilatation provides an excellent tool to better
understand the pathophysiologic mechanism of FMR.
2018 HKCBEES BANGKOK CONFERENCE
- 47 -
January 25, 2018 (Thursday)
Time: 16:30~18:30
Venue: LAROSE 1
Poster Session: 16 posters
Session Chair: Assoc. Prof. Win Chaeychomsri
B2005 Poster 8 (16:30~18:30)
Tissue Distribution of Copper in Mice Following Exposure to Arsenic in Drinking Water
Younghee Kim, Jiguk Kim and Kisok Kim
Keimyung University, Republic of Korea
Abstract—Arsenic (As) is a common and conspicuous toxicant. The tissue distribution and
correlation between tissues for copper was assessed in male C57Bl/6 mice exposed to 0, 50,
500, or 5000 ppb arsenic in their drinking water for 3 weeks. The copper concentration in
blood increased linearly with increasing arsenic doses, whereas the copper concentration in
liver or kidney decreased linearly with respect to the dose applied. These results indicate that
there may be tissue difference in the disturbed levels of copper in the mice blood, liver, and
kidney by subchronic exposure to arsenic in drinking water.
2018 HKCBEES BANGKOK CONFERENCE
- 48 -
January 25, 2018 (Thursday)
Time: 16:30~18:30
Venue: LAROSE 1
Poster Session: 16 posters
Session Chair: Assoc. Prof. Win Chaeychomsri
B0017 Poster 9 (16:30~18:30)
How to Predict Gait Speed and Ground Reaction Forces using Foot Pressure Sensors
Chang-Hyun Choi, Sun-Ok Chung and Joung Hwan Mun
Sungkyunkwan University, Korea
Abstract—The aim of this study was to predict ground reaction forces and gait speed
according to the plantar pressure data which obtained by insole type measurement device.
To predict gait speed and ground reaction forces of foot, the artificial neural network was
used in developing the models while in stance phase, swing phase for gait speed and nonlinear
models to obtain 3-axis of ground reaction moment in sagittal (GRFs), frontal (GRFF),
transverse (GRFT) and reaction ground force in medial-lateral (GRFML), anteriorposterior
(GRFAP), and vertical (GRFV) due to the fact that the vertical force can be obtainable from
plantar pressure data. There were three input variables of the prediction model of ground
reaction forces: accumulated plantar pressure which refers to time, the
pattern of center of pressure, and measured data of the opposite foot. These variables had
played significant role in improvement of the prediction performance of the nonlinear models.
To proceed the study, 80 young adult participants were gathered and performed normal
walking motions. All subjects were wearing portable insole-type measurement device which
composed of 99 pressure sensors. The predicted results of gait speed and ground reaction
forces were validated with actual data from a human motion capturing system (VICON 460)
and verified with five-fold cross-validation method. From the results, the correlation
coefficients showed high value for three different gait speeds (0.98, 0.97, and 0.95) and
showed high prediction accuracy for both prediction models in accumulated plantar pressure
and plantar pressure in opposed foot (r = 0.88-0.98) and accumulated plantar pressure and
center of pressure patterns (r = 0.91-0.99). Those findings were proposed that the methods in
this study are expected to be used to help in predicting various kinds of diseases and limited
spatial motions in ground reaction forces measurement process.
2018 HKCBEES BANGKOK CONFERENCE
- 49 -
January 25, 2018 (Thursday)
Time: 16:30~18:30
Venue: LAROSE 1
Poster Session: 16 posters
Session Chair: Assoc. Prof. Win Chaeychomsri
B0021 Poster 10 (16:30~18:30)
Near-infrared Spectroscopy Can be Used in Evaluation of Flesh Texture (Crunchiness) and
Water Content (Juiciness) of Apple Fruit
Jian-Zhi Chen and Sheng-Hao Tseng
National Cheng-Kung University, Taiwan
Abstract—Noninvasive optical methods have been widely applied to noninvasively
characterize the properties of turbid samples through measurements of photon-matter
interaction phenomena such as fluorescence or reflectance. Among various optical methods,
diffuse reflectance spectroscopy (DRS) has been demonstrated to be useful in apple quality
monitoring. For example, Cubeddu et al. employed a femto-second pulse laser source and
measured the diffuse reflectance containing pulse broadening effect caused by apples to
derive the absorption and scattering properties of apples. Durkin et al. illuminated apples
with striped patterns and record the diffuse reflectance images at various spatial frequencies
to determine the absorption and reduced scattering spectra of apples. Although DRS systems
have been used to retrieve the optical spectra of apples, the connection between the spectra
and the physical characteristics such as flesh texture and water content of apples has not been
established. In this study, we show that the near-infrared optical properties of apples
recovered from our frequency domain DRS system at the source-to-detector separation (SDS)
of 1 mm can reflect the properties of juiciness and crunchiness of apples. The system has the
potential for efficient and noninvasive grading the apples and other fruits.
2018 HKCBEES BANGKOK CONFERENCE
- 50 -
January 25, 2018 (Thursday)
Time: 16:30~18:30
Venue: LAROSE 1
Poster Session: 16 posters
Session Chair: Assoc. Prof. Win Chaeychomsri
B0024 Poster 11 (16:30~18:30)
Study on Water Quality in Tributaries of the Han River in Seoul
Sang-Hoon Lee and Hong-Kyu Ahn
Korea Institute of Civil Engineering and Building Technology, Korea
Abstract—The Seoul Metropolitan Government has performed a project that restores physical
shapes and ecological functions in damaged tributaries in Seoul continuously since 2000s. As
a result, ecological restoration in tributaries of the Han River has been conducted and stream
environments have changed variously.
Although water quality in streams has been improved overall, some streams have the
following problems despite of the ecological restoration project of tributaries.
First, the grade of water quality is still low even after ecological restoration. This was because
the project focused only on the restoration of a site of human living around the streams such
as maintenance of low flow revetment and high-water-level land by the river but the
contaminated sediments on the river bottom were not treated well thereby making a
continuous flow of contaminants into the streams and lowering the water quality grade.
Second, an increase in the coliform group was verified. For streams whose water quality has
improved, their mean water quality grade was Grade II (pH:6.5~8.5, BOD:≦3 mg/L, COD:≦
5 mg/L, TOC:≦4 mg/L, SS:≦25 mg/L, DO:≧5 mg/L, TP:≦0.1 mg/L, total Coliforms:≦
5,000MPN/100mL, Fecal coliforms:≦1,000MPN/100mL) except for the coliform group but
the overall water quality grade was Grade IV (pH:6.5~8.5, BOD:≦8 mg/L, COD:≦9 mg/L,
TOC:≦6 mg/L, SS:≦100 mg/L, DO:≧2 mg/L, TP:≦0.3 mg/L) due to the high
concentration of the coliform group. This was because sewage water was not intercepted and
separated by intercepting sewer pipes but directly flowed to the streams thereby increasing the
coliform group in the streams.
To solve this problem, first, improvements on water quality and sediment are needed, and
second, intercepting sewer pipes should be separated considering humans and stream
ecosystem during the ecological restoration of the streams. Thus, it is necessary for stream
restoration to aim for coexistence of human and nature by clarifying the goal of stream
restoration from the pre-plan phase.
2018 HKCBEES BANGKOK CONFERENCE
- 51 -
January 25, 2018 (Thursday)
Time: 16:30~18:30
Venue: LAROSE 1
Poster Session: 16 posters
Session Chair: Assoc. Prof. Win Chaeychomsri
B0025 Poster 12 (16:30~18:30)
Study on the Application of the Stream Environment Assessment Technique for Stream
Restoration Focusing on 30 Streams in Seoul Korea
Hong Kyu Ahn and Sang-Hoon Lee
Korea Institute of Civil Engineering and Building Technology, Korea
Abstract—The ecological restoration project of streams has been performed by the Ministry
of Environment and the Ministry of Land, Infrastructure, and Transport since 2000s to restore
ecological functions of the damaged streams. However, various problems occurred in the
ecological restoration project of streams because the project was focused only on making the
shape of the streams closer to that of nature without concentrating on true restoration of the
stream ecosystem.
One of the significant causes of this failure is due to no clear assessment and diagnosis on the
current status of the streams. More specifically, since the restoration direction and goals for
streams were not set up clearly from the planning phase, the stream restoration was conducted
focusingon humans rather than ecology-oriented restoration.
Furthermore, although more than 440 million dollar as been invested annually for the
ecological restoration project of streams, no systematic assessment system has been
constructed and utilized as to whether the stream environment was truly improved more than
that prior to the project or if improved, how much the environment was improved.
Thus, an opinion has been raised that a quantitative assessment on the streams was needed to
restore and maintain the ecological streams systematically.
This study evaluated the stream environment quantitatively utilizing the stream environment
assessment system with regard to 30 streams where the ecological restoration project of
streams have already been conducted or will be conducted. The stream environment
assessment has been performed comprehensively in the following five areas: physical
characteristics, water quality characteristics, stream naturalness, aquatic ecosystem health, and
citizen satisfaction.
The study results showed that the stream that had the highest grade in the stream evaluation
was selected as the reference river of the ecological restoration project of streams in Seoul
Metropolitan Government whereas the stream that had the lowest grade was selected as the
priority target river of the project.
2018 HKCBEES BANGKOK CONFERENCE
- 52 -
January 25, 2018 (Thursday)
Time: 16:30~18:30
Venue: LAROSE 1
Poster Session: 16 posters
Session Chair: Assoc. Prof. Win Chaeychomsri
B0028 Poster 13 (16:30~18:30)
The Impact of PDDA in CNT Counter Electrode on the Conversion Efficiency of DSSC
Yoshiki Kurokawa, Dang Trang Nguyen and Kozo Taguchi
Ritsumeikan University, Japan
Abstract—Dye-Sensitized Solar Cell (DSSC) is one of the equipment to convert sunlight into
electricity. DSSC consists of anode electrode, titanium oxide ( ) thin film, sensitizer dye,
electrolyte and cathode electrode (CE). In general, platinum (Pt) is usually used for CE. This
paper studied the fabrication of CE using carbon nanotube (CNT) and CNT mixed with Poly
(diallyl dimethylammonium chloride) (PDDA) (CNT/PDDA) as the substitution for Pt
electrode. For the fabrication process, Electrophoresis Deposition (EPD) method was
employed. CNT electrode was dried at room temperature at 25 oC or annealed at 400 oC for 1
hour, and CNT/PDDA CE was dried at room temperature at 25 oC. The thickness of CE we
fabricated was around 15 . We adapted MK2 dye which was an organic dye for DSSC.
Experimental results showed that the conversion efficiency of the DSSC in the case of
CNT/PDDA CE (4.12%) was higher than that of the case of dried CNT electrode (2.78%)
and annealed CNT electrode (3.49%) under the 1 sun illumination (100 ). From this
experiment results, PDDA has improved CE ability. In addition, CNT/PDDA CE do not need
annealing process, so enabling flexible DSSC.
2018 HKCBEES BANGKOK CONFERENCE
- 53 -
January 25, 2018 (Thursday)
Time: 16:30~18:30
Venue: LAROSE 1
Poster Session: 16 posters
Session Chair: Assoc. Prof. Win Chaeychomsri
Poster 14 (16:30~18:30)
Shallow HVs and Geothermal Energy for Heating Purposes in Aquaculture
Hans-Uwe Dahms
Kaohsiung Medical University, Taiwan
Abstract—The objective of this study is to examine whether heating of aquaculture tank or
net-cages with geothermal heat from shallow hydrothermal vents (HVs) or geothermal heat
pumping (GHP) systems is feasible. Whereas shallow HVs are globally rather sparsely
distributed, geothermal energy is available worldwide, coastal or inland. The success and
economic benefits of aquaculture operations with low-temperature caused the question
whether or not lower temperature resources could be exploited with the aid of geothermal heat
pumps (GHPs). This study seeks to answer that question, and, therefore, the objective is to
determine the feasibility of heating fish tanks with GHP systems. Aquaculture involves the
raising of freshwater or marine organisms in a controlled environment to enhance production
rates. It has been demonstrated that more fish can be produced in a shorter period of time if
geothermal energy is used for aquaculture pond and raceway heating rather than water
depending on the sun for physical heating. When the water temperature falls below optimal
values, the fish lose their ability to feed because their basic body metabolism is affected. A
good supply of geothermal water, by virtue of its constant temperature, can, therefore,
optimize even a naturally mild climate. Optimum temperature is generally more important for
aquatic species than land animals, which suggests that the potential of geothermal energy in
aquaculture may be greater than in animal rearing, such as chicken and pig and chicken
husbandry. The use of low-temperature geothermal resources for space heating is quite
straightforward. If the geothermal fluid is corrosive or even slightly toxic, a plate heat
exchanger can isolate the fluid from the greenhouse heating equipment. Other alternatives
(biofuel, photovoltaic, wind) or fossil fuel peaking can be combined with the geothermal heat
in case of depleted geothermal resources (cold periods, seasonally, or at night). Strategic
importance of energy sources that are locally available for food production, and using a
geothermal resource in combination with alternative heating fossil or renewable systems for
peak heating.
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January 25, 2018 (Thursday)
Time: 16:30~18:30
Venue: LAROSE 1
Poster Session: 16 posters
Session Chair: Assoc. Prof. Win Chaeychomsri
B4004 Poster 15 (16:30~18:30)
Biomimetic Tactile Information Processing for Surface Roughness Discrimination
Qin Longhui and Zhang Yilei
The School of Mechanical and Aerospace Engineering, Nanyang Technological University,
Singapore
Abstract—Different from texture classification, surface roughness discrimination is more
challenging in the development of tactile sensing because of limited discriminative
information. In recent years, it is receiving more and more attentions from researchers in
various fields, most of which are based on supervised learning. But frequently all we have is
unlabeled dataset with very limited prior information, i.e., labels are not available to train the
discrimination models. Lacking the ‗teaching‘ process, it becomes rather difficult to locate the
boundary of different classes. In this paper, the ability of unsupervised surface roughness
discrimination is explored based on our developed bio-inspired artificial fingertip. At first, the
original signals are analyzed and discriminated with the most widely used unsupervised
algorithm (K-means clustering). Then the technique of discrete wavelet transform and
algorithm of sequential forward selection are utilized to identify the most discriminative
features. The unsupervised discrimination results (K-means clustering) are presented and
compared based on different distances including Squared Euclidean, Cityblock, and Cosine.
The highest test accuracy reaches 72.93%±12.55% when the distance of Squeared Euclidean
is adopted with six discriminative features. Finally, another popular unsupervised algorithm,
self-organizing maps neural network that is different from clustering, is also applied in
discriminating surface roughness with lower accuracy. The results show that unsupervised
learning algorithms with our developed tactile fingertip are capable to discriminate surface
roughness, which have great potentials in robotics and autonomous applications.
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January 25, 2018 (Thursday)
Time: 16:30~18:30
Venue: LAROSE 1
Poster Session: 16 posters
Session Chair: Assoc. Prof. Win Chaeychomsri
B0019 Poster 16 (16:30~18:30)
Effects of Low Soil Temperature on Antioxidant Enzyme System of Rice under Drip
Irrigation
Jun Zhang, Peng Dong and Changzhou Wei
Agriculture College, Shihezi University, China
Abstract—Rice cultivate by drip irrigation is a newly proposed water-saving rice cultivation
method. Drip irrigation (DI) rice grower found low temperature well water cause rice yield
decrease sharply, but flooding irrigation (FI) was affect not so severe. This study mainly
discusses the main reason that rice in DI was more sensitive to soil temperature than FI. The
study consisted of a two factor design with three soil temperature (18°C, 24°C and 30°C) and
two irrigation methods (flooded and drip irrigation). The results showed that the
photosynthesis intensity of rice in DI was decreased at 30℃ soil temperature,the activity of
SOD and CAT in DI was increased by 5% and 23%, Pro content increased by 27%. All data
showed rice in FI was suffer certain stress. Low soil temperature (18℃, 24℃) increased the
stress of rice in DI. Compared with FI, secondary branches and spikelet differentiation
number of rice in DI decreased significantly, protective enzymes increased more in DI than in
FI, Therefore, low temperature and drought stress may be the main reason for the serious
decline of rice in DI at low temperature.
Dinner 18:30
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Listeners Name Affiliation Hongsun-Yun Research and Development Institute, Daesung-Maref CO. LTD.,
Korea Moonki Kim Research and Development Institute, Daesung-Maref CO. LTD.,
Korea Hiroyuki Kudo Meiji University, Japan Michael James University of Adelaide, Australia Andiyanti Putri
Estigade Universitas Gadjah Mada, Indonesia Ariani Puji Astuti Universitas Gadjah Mada, Indonesia Dea Nadia Universitas Gadjah Mada, Indonesia
Muhammad Hilmy
Aziz Universitas Gadjah Mada, Indonesia
Tika Maitela Universitas Gadjah Mada, Indonesia
2018 HKCBEES BANGKOK CONFERENCE
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One Day Visit Time: 6:30~16:00 January 26, 2018(Friday)
(Tip: The following places are for references, and the final schedule should be adjusted to the
actual notice.)
6:30 Gathering in CHATEAU DE BANGKOK‘s Lobby
1. 8:30~9:30 Wat Phu Khao Thong
Wat Phukhao Thong or the Monastery of the Golden Mount is
located off the city island in the plains 2 Km northwest of
Ayutthaya in Phukhao Thong Sub-district.
The actual temple‘s name refers to the high chedi on its north east
side. At the time of construction of the monastery, this chedi was
inexistent; hence the temple probably had
another name.
2. 9:30~12:00 Wat Phra Sri Sanphet
Wat Phra Sri Sanphet is located in Pratu Chai subdistrict, Phra
Nakorn Si Ayutthaya district, Ayutthaya province. The temple is
not only a significant historical site, but also considered as the
spiritual center of Thais for a long time.
3. 12:00~14:00 Lunch
4. 14:00~14:30 Wat Pho
Wat Pho, also spelt Wat Po, is a Buddhist temple complex in the Phra Nakhon District, Bangkok,
Thailand. It is on Rattanakosin Island, directly south of the Grand Palace.Known also as the
Temple of the Reclining Buddha, its official name is Wat Phra Chetuphon Vimolmangklararm
Rajwaramahaviharn
5. 14:30~15:00 Bang Pa-In Summer Palace
Bang Pa-In Royal Palace, also known as the Summer Palace, is a
palace complex formerly used by the Thai kings. It lies beside the
Chao Phraya River in Bang Pa-in District, Phra Nakhon Si Ayutthaya
Province.
6. 15:00 Go back to Bangkok, Thailand
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Conference Venue
CHATEAU DE BANGKOK http://www.chateaubkk.com/
Address: 29 Soi Ruamrudee , Ploenchit Road, Lumpini, Patumwan - 10330 Bangkok
Centrally located in the shopping and business districts, the hotel is just a short distance from major
shopping malls, embassies, office towers and the main BTS Skytrain public transport.
2018 HKCBEES BANGKOK CONFERENCE
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