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©2013 MFMER | slide-1 Xenoantigenicity of Porcine Decellularized Valves MRK Helder MD, R Hennessy...
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Transcript of ©2013 MFMER | slide-1 Xenoantigenicity of Porcine Decellularized Valves MRK Helder MD, R Hennessy...
![Page 1: ©2013 MFMER | slide-1 Xenoantigenicity of Porcine Decellularized Valves MRK Helder MD, R Hennessy MD, R Dyer PhD, T Witt CVT, DB Spoon MD, RD Simari MD,](https://reader035.fdocuments.net/reader035/viewer/2022070401/56649f1b5503460f94c300a8/html5/thumbnails/1.jpg)
©2013 MFMER | slide-1
Xenoantigenicity of Porcine Decellularized ValvesMRK Helder MD, R Hennessy MD, R Dyer PhD, T Witt CVT, DB Spoon MD, RD Simari MD, JM Stulak MD, A Lerman MD
American Association for Thoracic SurgeryApril 28, 2105
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©2013 MFMER | slide-2
Disclsoure
• Funding – Dontation from H.H. Sheikh Hamed Bin Zayed Sultan Al-Nahyan
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©2013 MFMER | slide-3
BackgroundXenoantigenicity of Porcine Decellularized Valves
• Bioprosthetic valves fail• immune response?
• Decellularization should blunt
• α gal vs non α gal xenoantigens
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©2013 MFMER | slide-4
MethodsXenoantigenicity of Porcine Decellularized Valves
• Porcine aortic valves decell with 1% SDS
• Implanted into 3 sheepopulmonary position
• Serum obtained oPreopo1 wko1 moo2 mo
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©2013 MFMER | slide-5
A B C D
E F G H
Preop 1 week 1 month 2 months
Por
cine
Ovi
neResultsAntibody Reaction
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©2013 MFMER | slide-6
0
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Time Point
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g/m
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ResultsQuantitative Antibody Reaction
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©2013 MFMER | slide-7
Preop
Postop
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/ml *
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ResultsComplement
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©2013 MFMER | slide-8
A B C
C D
+ Control Sheep C
D8
Isot
ype
Con
trol
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©2013 MFMER | slide-9
SummaryXenoantigenicity of Porcine Decellularized Valves
• Evidence of IgG and IgM reaction
• Increased complement concentrations
• No T-lymphocyte role
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©2013 MFMER | slide-10
Conclusions
• Antibody and complement reaction to xeno valves
• Clinical implications are unclear and require further study
• SDS decellularization does not remove non-alpha gal antigens
• New products must be evaluated for antigenicity to ensure durability
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©2013 MFMER | slide-11
©2011 MFMER |
3149555-11
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