2012 HOT I TEM 20% Clontech 전품목 10 ~ 20% 할인 전제품...

www.takara.co.kr

Clontech 전품목 10 ~ 20% 할인

관련 제품

In-Fusion HD Cloning 시리즈 할인

제한효소 사이트 구애없이, subcloning없이 원하는 vector에 directional cloning

제품명 Code 용량 특징 캠페인가

Stellar Competent Cells 636763 10 x 100 ㎕ 형질전환 효율 : > 5 × 108 cfu/μg 264,000

CloneAmp HiFi PCR Premix 639298 200회 ≤ 10 kb 증폭시 최적의 high fidelity PCR 효소 288,000

Advantage HD Polymerase Mix 639241 200회 ≤ 8 kb 증폭시 최적의 high fidelity PCR 효소 247,000

기간 : ~ 11월 22일까지C L O N T E C H C A M P A I G NT H E T R A N S F E C T I O N E X P E R T S

In-Fusion Cloning 제품

정제 : CE(Cloning Enhancer ), NS(NucleoSpin) Cells : Stellar Competent Cells PCR 효소 : CloneAmp HiFi PCR Premix* Liquid type 용량은 10회, 50회, 100회이며, Drydown type은 8회, 24회, 96회로 구성됨 (자세한 제품 리스트는 홈페이지 참조)

분 류 제 품 명 Code 용량 정제 Cells PCR 효소 캠페인가

Liquid Type

In-Fusion HD Cloning Plus 638909 10 회 NS O O 302,000

In-Fusion HD Cloning Plus CE 638916 10 회 CE O O 302,000

In-Fusion HD Cloning Kit w/Cells 639642 10 회 - O - 236,000

In-Fusion HD Cloning Kit 639648 10 회 - - - 196,000

Drydown Type

In-Fusion HD EcoDry Cloning Plus 638912 8 회 NS O O 275,000

In-Fusion HD EcoDry Cloning Kit w/Cells 639678 8 회 - O - 231,000

In-Fusion HD EcoDry Cloning Kit 639689 8 회 - - - 189,000

www.TheTransfectionExperts.com

제품명 Code 용량 캠페인가

Plasmid DNA와 siRNA에 적용 가능한 Broad Trasnfection 시약

TransIT-X2 Dynamic Delivery System MIR 6000 1.5 ml 466,200

Broad Transfection 시약

TransIT-LT1 Transfection Reagent MIR 2300 1 ml 358,400

TransIT-2020 Transfection Reagent MIR 5400 1 ml 458,400

Biotherapeutic Protein 생산에 이상적인 Trasnfection 시약

TransIT-PRO Transfection Reagent MIR 5700 1 ml 454,400

Small RNA Transfection 시약

TransIT-TKO Transfection Reagent MIR 2150 1.5 ml 556,800

TransIT-siQUEST Transfection Reagent MIR 2110 1.5 ml 556,800

다양한 Cell Line 특이적 Plasmid DNA Trasnfection 시약 보유

Cell type에 적합한 Transfection reagent가 궁금하다면 ?

기간 : ~ 11월 22일

Transfection Reagent 시리즈첫구매 40% 할인

(홈페이지 공지사항 참조)

TransIT-X2

Labeling kit 시리즈

* 표기된 제품 이외에 다양한 non-RI Labeling kit 보유

microRNA Labeling kit

Label IT miRNA Labeling Kit, Cy3/Cy5 MIR 9305 2 x 5 reactions 407,200

Label IT miRNA Labeling Kit, Biotin MIR 9410 10 reactions 407,200

Intracellular Nucleic Acid Localization - DNA

Label IT Tracker Intracellular Nucleic Acid Localization, Cy3 MIR 7020 Labels 50-200 ug 296,800

Label IT Tracker Intracellular Nucleic Acid Localization, Cy5 MIR 7021 Labels 50-200 ug 296,800

Intracellular Nucleic Acid Localization - siRNA

Label IT siRNA Tracker Intracellular Localization, Cy3 MIR 7212 Labels 50 ug 500,000

Label IT siRNA Tracker Intracellular Localization, Cy5 MIR 7213 Labels 50 ug 500,000

FISH에 적용할 수있는 Labeling kit

Label IT FISH Labeling kit, Cy3 MIR 6510 labels 10 ug 996,800

Label IT FISH Labeling kit, Biotin MIR 6514 labels 10 ug 952,000

• Fast, easy, directional cloning

or phosphatase required

of up to 12 kb

restriction

(Figure

1; available

formats,

reactions

to dry-down

consistency

liquid

format

Cloning

NucleoSpin® so you

for your For further

provides a wide complement the

researchers who they would

In-Fusion with options

6xHN tags proteins,

expression can transform

highly Competent

use with technologies.

Cloning features cloning

In-Fusion, one or any vector,

producing

The In-Fusion™ AdvantageA system you can use with any vector and insert—the single solution for all your PCR cloning applications

Table I: In-Fusion PCR Cloning vs. Other Cloning Systems

Limitations of Other Cloning SystemsThe In-Fusion Advantage

• Vector choice is limited.

• Different kits require different vectors.

• Must use vector supplied with kit.

This versatile technology allows the cloning of ANY PCR product into ANY linearized vector, as long as they share 15 bp of homology at each end.

• Restriction digestion and ligation of inserts is necessary.

• Require unique, compatible restriction sites.

• Appropriate restriction sites are limited.

No restriction digestion or ligation of PCR products is required. Cloning is not hindered by restriction sites within the PCR products.

• Complex constructs require subcloning.No subcloning is required.

• Procedures can be time-consuming, requiring multiple individual adjustments.

In just one 30 minute reaction, multiple PCR products can be cloned into any vector.

• Limited range of acceptable insert sizes.DNA of up to 12 kb; see Tables II and III.

• Nondirectional cloning requires the screening of constructs for correct insert orientation.

Cloning is directional, so the insert is always in the desired orientation.

• Extra or unwanted base pairs are often Cloning is seamless; no unwanted base pairs are added to the construct.

• Not suited to mid- and large-scale cloning projects.

In-Fusion cloning technology is easily automated.

Figure 1. The In-Fusion Advantage Cloning Protocol.

gene of interest with 15 bp extensions homologous to the ends of vector. The PCR product

in a 30 min In-Fusion Advantage cloning reaction. The cloning reaction is then used to transform E. coli.

Amplify your gene of interests

with 15 bp extensionshomologous to vector ends

Anylinearized

vector

Recombinant vector

30 min single-tubereaction

The In-Fusion Enzyme creates

which are then fused dueto the

Add proprietary

to the PCR productOR

PCR product

2 0 1 2 H O T I T E M

In-Fusion® PCR CloningCloning=Clontech

제한효소 사이트 관계없이 Subcloning없이 원하는 Vector에 Directional Cloning

① Standard Cloning ② Multiple Insert Cloning

③ Long Insert Cloning ④ Site-Directed Mutagenesis

⑤ BioBrick Construction

복수의 DNA 단편을 1회의 cloning 반응으로

원하는 clone을 얻을 수 있다.

In-Fusion primer를 활용하면

point mutation 제작에도 유용하다.

Multiple Insert Cloning

Fragment 1Fragment 2

Linearizedvector

Fragment 3Fragment 1

Fragment 2

Linearizedvector

Fragment 3

Fragment 4

Site-Directed Mutagenesis

mutation

In-Fusion Cloning을 이용한 다양한 실험 적용

In-Fusion 반응(50℃, 15분)

Insert 준비 : In-Fusion primer로 PCR

In-Fusion primer는 gene specific primer( )에

vector의 5＇말단과 상보적 서열(※：15 bp)을 부가한

primer이다.

대리점 정보

그린비바이오 (주) 02-2633-7114

(주) 에스앤씨 1644-5778

다카라코리아대전지사 042-828-6525 (주) 삼화교역 063-227-3700

(주) 진성에스엠알 062-672-7631

(주) 프라임텍 033-244-6969

브니엘바이오 053-381-3611

(주) 대한과학 051-247-2875

이엔바이오 055-761-1036

(주) 셀피스 064-722-6707

Vector 준비 : vector 선형화 (linearize)

• 제한효소 처리 : 2 종류의 제한효소로 처리하는 경우 더 효과적이다

• Inverse PCR : PCR로 vector를 증폭 증폭시 high-fildelity PCR 효소 사용을 권장한다.

PCR product 정제 : Cloning Enhancer나 NucleoSpin으로 권장

목적유전자(insert)

강원(주)프라임텍

대구, 경북브니엘바이오

부산, 울산, 경남(주)대한과학

진주이엔바이오

대전, 충청다카라코리아대전지사

전북(주)삼화교역

광주, 전남(주)진성에스엠알

서울, 경기, 인천그린비바이오(주)(주)에스엔씨

제주 (주)셀피스

A system you can use with any vector and insert—the single solution for all your PCR cloning applications

Table I: In-Fusion PCR Cloning vs. Other Cloning Systems

Limitations of Other Cloning SystemsThe In-Fusion Advantage

Different kits require different vectors.

Must use vector supplied with kit.

This versatile technology allows the cloning of ANY PCR product into ANY linearized vector, as long as they share 15 bp of homology at each end.

Restriction digestion and ligation of inserts

Require unique, compatible restriction sites.

Appropriate restriction sites are limited.

No restriction digestion or ligation of PCR products is required. Cloning is not hindered by restriction sites within the PCR products.

Complex constructs require subcloning.No subcloning is required.

Procedures can be time-consuming,requiring multiple individual adjustments.

In just one 30 minute reaction, multiple PCR products can be cloned into any vector.

Limited range of acceptable insert sizes.DNA of up to 12 kb; see Tables II and III.

Nondirectional cloning requires the screening of constructs for correct

Cloning is directional, so the insert is always in the desired orientation.

Extra or unwanted base pairs are often Cloning is seamless; no unwanted base

Figure 1. The In-Fusion Advantage Cloning Protocol.

gene of interest with 15 bp extensions homologous to the ends of vector. The PCR product

in a 30 min In-Fusion Advantage cloning reaction. The cloning reaction is then used

Amplify your gene of interests

with 15 bp extensionshomologous to vector ends

Anylinearized

vector

Recombinant vector

30 min single-tubereaction

single-stranded regions at the endsof the vector and PCR product,

15 bp homology

In-Fusion 효소가 vector와PCR product(insert) 말단을single-strand으로 만든 후15bp의 상동서열을 융합시킨다.

Liquid type정보 EcoDry type 정보 브러셔In-Fusion Cloning을 이용한 다양한 실험 적용

① Standard Cloning ② Multiple Insert Cloning ③ Long Insert Cloning ④ Site-Directed Mutagenesis ⑤ BioBrick Construction

* 표기된 캠페인가는 부가세별도 금액입니다. 자세한 제품 정보는 홈페이지 참조

Mirus 전제품 20%

대 전 지 사 042-828-6525 고 객 (제품문의) 02-2081-2510

지원센터 [email protected] (대전•충청지역 제품주문)

지역별 전문 대리점

그린비바이오 (주) (서울,인천,경기) 02-2633-7114

(주) 에스앤씨 (서울,인천,경기) 1644-5778

(주) 삼화교역 (전북) 063-227-3700

(주) 진성에스엠알 (광주, 전남) 062-672-7631

(주) 프라임텍 (강원) 033-244-6969

브니엘바이오 (대구, 경북) 053-381-3611

(주) 대한과학 (부산, 울산, 경남) 051-247-2875

이엔바이오 (진주) 055-761-1036

(주) 셀피스 (제주) 064-722-6707

1 0 % ~ 3 5 % O F F S A L E

www.clontech.com

높은 증폭효율, 편리성을 갖춘 동결건조 Premix 역전사 효소

EcoDry RNA to cDNA Premix 시리즈 20% 할인

1~2 ng의 total RNA로부터 Full-length cDNA 합성

SMARTer PCR cDNA Synthesis & SMARTer RACE 시리즈

량 용 edoC 명 품 제 류 분 캠페인가

Illumina NGS SMARTer Ultra Low RNA Kit for Illumina Sequencing 634935 10 회 2,208,000

cDNA 합성000,742,1 회 01 529436 tiK sisehtnyS ANDc RCP reTRAMS

000,349,1 회 01 829436 tiK sisehtnyS ANDc RCP ociP reTRAMS

RACE000,902,1 회 01 329436 tiK noitacilpmA ANDc ECAR reTRAMS

000,191,1 ANDc 5 319436 tiK noitacifilpmA ANDc nohtaraM

Library In-Fusion SMARTer Directional cDNA Library Construction Kit 634933 10 회 1,980,000

000,616,1 회 01 109436 tiK noitcurtsnoC yrarbiL ANDc TRAMS

량 용 edoC 명 품 제 캠페인가

639543 24 rxns 196,000

000,292 snxr 84 145936 )Td ogilO( ximerP yrDocE ANDc ot ANR

639542 96 rxns 495,000

639546 24 rxns 196,000

,292 snxr 84 445936 )sremaxeH modnaR( ximerP yrDocE ANDc ot ANR 000

639545 96 rxns 495,000

639549 24 rxns 196,000

0,292 snxr 84 745936 )*demirP elbuoD( ximerP yrDocE ANDc ot ANR 00

639548 96 rxns 495,000

Low background, High Sensitivity

Tet-On 3G Tetracycline Inducible Gene Expression Systems

◦ 낮은 background

◦ Doxycycline에 대한 민감도 증가

◦ 다양한 형태의 vector format

◦ SMART (Switching Mechanism At the 5’ end of RNA Transcript) 기술

◦ Single cell 수준의 극소량의 RNA로 full length cDNA 합성

◦ 추가 실험(T4 ligase처리, adaptor ligation, 정제 등) 불필요

Background를 줄이기 위해 functional test가 완료된

Clontech의 Tet System Approved FBS 권장 (Code 631106)

* 제품리스트는 홈페이지 참고

* 반응액에 Oligo dT와 Rondom Hexamers가 함께 포함되어 있다.

Tet-On 3G transactivator protein을 발현하며 TRE3G promoter (PTRE3G)로 목적 유전자를

조절하는 Tet-On 3G 세포는 doxycycline (Dox)이 첨가되었을 때 목적 유전자를 고발현 시킨

다. Tet-On 3G protein은 Dox가 결합하면 형태의 변화가 생겨 PTRE3G 내의 tet operator (tet O)

에 결합하게 된다

전 품 목 1 0 ~ 2 0 % 할 인C L O N T E C H C A M P A I G N

Lentiviral Delivery를 위한 노하우 총집합 ~ 할인

EcoDry RNA to cDNA Premix 시리즈 할인높은 증폭효율, 편리성을 갖춘 동결건조 타입의 premix 역전사 효소

Lowest background, highest sensitivity, and highest induction

C l o n t e c h C a m p a i g n

www.takara.co.kr / m.takara.co.kr

)분 51(응반 noisuF-nI .2폭증 RCP .1

Vector 말단 서열의 15 bp를 목적 DNA 증폭용 primer에 첨가하여 설계

Insert의PCR 증폭

In-Fusion 효소

목적 Vector

형질전환

Vector의제한효소처리or PCR 증폭

목적 DNA와 vector를 각각 PCR 증폭할 때

vector 말단의 약 15 bp를 목적 DNA 증폭용

primer에 추가하여 제작하는 것이 핵심이다.

(vector의 선형화는 제한효소 처리 가능)

증폭한 목적 DNA와 선형화 vector를 혼합하고

In-Fusion enzyme을 첨가하여 반응한다.

Clontech 독자적인 In-Fusion 효소가 약 15염기의

상동 부분을 융합한다.

Subcloning 없이 vector에 directional cloning이

가능하다. 상동 서열 부분은 원래 vector에 있던

서열이므로, 추가 서열의 부가는 일어나지 않는다.

분 류 소효 RCP slleC 제정 량 용 edoC 명 품 제 캠페인가

Liquid Type

000,303 O O SN 회 01 909836 sulP gninolC DH noisuF-nI

000,303 O O EC 회 01 619836 EC sulP gninolC DH noisuF-nI

000,632 - O - 회 01 246936 slleC/w tiK gninolC DH noisuF-nI

000,632 - - SN 회 01 936936 SN/w tiK gninolC DH noisuF-nI

000,632 - - EC 회 01 336936 EC/w tiK gninolC DH noisuF-nI

000,691 - - - 회 01 846936 tiK gninolC DH noisuF-nI

EcoDry

000,981 - - - 회 8 986936 tiK gninolC yrDocE DH noisuF-nI

정제 : NS(NucleoSpin), CE(Cloning Enhancer) Cell : Stellar Competent Cell PCR 효소 : CloneAmp HiFi PCR Premix

CloneAmp HiFi PCR Premix(Code 639298)은 2x MasterMix제품으로 높은 정확도와 뛰어난 증폭효율을 지닌 high fidelity PCR효소로 In-Fusion Cloning에 사용하기에 적합하다.

제한효소 사이트 구애없이, subcloning없이 원하는 vector에 클로닝

In-Fusion HD Cloning 시리즈 20% 할인

Packaging

000,044,1 회 02 742136 metsyS gnigakcaP XTH X-itneL

System

44,1 회 02 152136 metsyS gnigakcaP ciportocE XTH X-itneL 0,000

Lenti-X HTX Packaging System (Integrase Deficient) 631258 20 회 1,434,000

000,684 lm 1 081236 eniL lleC T392 X-itneL

Titeration

000,333 회 02 342136 xitSoG X-itneL

00,675 회 002 932136 tiK noitatitnauQ surivorP X-itneL 0

000,085 회002 532136 tiK noitartiT RCP-TRq X-itneL

000,556 회 69 002236 tiK retiT dipaR 42p X-itneL

00,062 lm 001 132136 rotartnecnoC X-itneL noitartnecnoC 0

3 회 2 332136 tiK noitacifiruP ixaM X-itneL noitacifiruP 28,000

000,259 lm 1 752136 rotareleccA X-itneL noitcudsnarT

Lentivirus 제작을 위한 Full Line-up

Lenti-X

expression SystemPackaging System Titeration

Concentration

PurificationTransduction Provirus 분석

일반 vector, Tet-Inducible용, 형광단백질포함, EF1-α promoter 등 다양한 vector 보유

분류 Code 품목명 용량 캠페인가

Lentiviral Vector 다수 다양한 포맷의 Lentiviral vector 보유 10% 할인

Packaging

631247 Lenti-X HTX Packaging System 20 회 1,440,000

System

631251 Lenti-X Ecotropic Packaging System 20 회 1,440,000

631258 Lenti-X HTX Packaging System (Integrase Deficient) 20 회 1,434,000

632180 Lenti-X 293T Cell Line 1 ml 486,000

Titeration

631243 Lenti-X GoStix 20 회 333,000

631235 Lenti-X qRT-PCR Titration Kit 200 회 579,000

632200 Lenti-X p24 Rapid Titer Kit 96 회 654,000

Concentration 631231 Lenti-X Concentrator 100 ml 259,000

Purification 631233 Lenti-X Maxi Purification Kit 2 회 328,000

Transduction 631254 Lenti-X Accelerator Starter Kit each 1,104,000

Enhancer 631471 Ecotropic Receptor Booster 20 회 357,000

Provirus 분석 631239 Lenti-X Provirus Quantitation Kit 200 회 576,000

631263 Lenti-X Integration Site Analysis Kit each 1,132,000

분류 제품명 Code 용량 캠페인가

NGS SMARTer Ultra Low Input RNA for Illumina Sequencing-HV 634820 12 회 2,553,000

SMARTer Universal Low Input RNA Kit for Sequencing* 634938 10 회 2,208,000

cDNA SMARTer PCR cDNA Synthesis Kit 634925 10 회 1,247,000

합성 SMARTer Pico PCR cDNA Synthesis Kit 634928 10 회 1,942,000

RACE SMARTer RACE cDNA Amplication Kit 634923 10 회 1,209,000

Marathon cDNA Amplification Kit 634913 5 cDNA 1,191,000

SMART cDNA Library Construction Kit 634901 10 회 1,616,000

RNA to cDNA EcoDry Premix (Oligo dT)

639543 24 rxns 196,000

639541 48 rxns 291,000

639542 96 rxns 494,000

RNA to cDNA EcoDry Premix (Random Hexamers)

639546 24 rxns 196,000

639544 48 rxns 291,000

639545 96 rxns 494,000

RNA to cDNA EcoDry Premix (Double Primed*)

639549 24 rxns 196,000

639547 48 rxns 291,000

639548 96 rxns 494,000

A Takara Bio Company

www.clontech.com

Clontech Laboratories, Inc.

1212 US (633057)

United States/Canada: +1.800.662.2566 • Asia Pacific: +1.650.919.7300 • Europe: +33.(0)1.3904.6880 • Japan: +81.(0)77.543.724For Research Use Only. Not for use in diagnostic or therapeutic procedures. Not for resale. Clontech®, the Clontech logo, Advantage, EcoDry, SMART, and Titanium are trademarks of Clontech Laboratories, Inc. All other marks are the property of their respective owners. Certain trademarks may not be registered in all jurisdictions.©2012 Clontech Laboratories, Inc.

P C R , R T - P C R , & R E A L - T I M E q P C R

RNA to cDNA EcoDry Premix

Our RNA to cDNA EcoDry Premix allows first-strand cDNA synthesis with the

convenience and flexibility of the EcoDry format.

• Add RNA and go!

• Features our high-yield SMART™ MMLV Reverse Transcriptase (RT)

• Synthesize high-quality, full-length cDNA from virtually any transcript

• Each tube of master mix includes SMART MMLV RT, dNTPs, and your

choice of oligo(dT)18 primers, random hexamer primers, or a combination

of both

Benefits of EcoDry Premixes

• Pre-aliquoted mixes are ready-to-use

• Single-use tubes minimize contamination

• Simple protocol with minimal hands-on time

• Features our high-performance enzymes

• Convenient room-temperature storage

• Flexible formats - single to 96 reactions

EcoDry lyophilized master mixes are stable at room temperature, allowing DNA amplifica-tion and first-strand cDNA synthesis with minimal effort and maximum ease. This convenient format also minimizes waste in storage and shipping, making it the eco-friendly choice.

EcoDry premixes are packaged in 8-well tube strips with clear sealing caps.

EcoDry™ PCR & Reverse Transcription PremixesEasy, Convenient, and Foolproof RT and PCR

Eco-friendly Single-use Ready-to-go Robust

* Oligo dT와 Random Hexamers가 동시에 포함되어 있다.

* 리스트 외에 다양한 format의 제품 보유 (자세한 리스트는 홈페이지 참조)

* Random primer를 사용하여 non-polyA RNA(bacteria RNA) sample 적용 가능

SMARTer cDNA synthesis occurs in a singlestep reverse transcription reaction. Following

amplification, SMARTer cDNA can be used for a variety of downstream applications.

First-strandcDNA synthesis

SMARTeroligo

Oligo(dT) primerwith added

PCRadaptor

poly A

5' poly A

SMARTer cDNA Synthesis

LD-PCR amplificationor primer extension

Second-strandcDNA synthesis

Full-lengthds cDNA

Startingmaterial

Singlestep

Templatefor real-time

Next-generation

Direct geneamplification

Libraryconstruction

SMARTScribe MMLV RT

obesfor arrays

Subtractivehybridization

NA the SMARTer™ Way

NA from as little as 1–2 ng of total RNAles and maintain accurate gene representation

wer background, and increased yieldNA

necessarynger genes and rare transcripts

MARTer chnology

MARTer RACE cDNA Ampli�cation Kit

www.clontech.com

I n d u c I b l e S y S t e m S

6.13 IN (633209)

United States/Canada: +1.800.662.2566 • Asia Pacific: +1.650.919.7300 • Europe: +33.(0)1.3904.6880 • Japan: +81.(0)77.543.724For Research Use Only. Not for use in diagnostic or therapeutic procedures. Not for resale. Clontech, the Clontech logo, and Tet-On are trademarks of Clontech Laboratories, Inc. All other marks are the property of their respective owners. Certain trademarks may not be registered in all jurisdictions. ©2013 Clontech Laboratories, Inc.

600ReducedBackground

Tet-On® 3G Inducible Expression SystemsLowest background, highest sensitivity, and highest induction

Lowest-Ever Background, Highest Sensitivity

The combination of the following two optimized elements makes Tet-On 3G

better than previous generations of the Tet-On Inducible Expression System:

1. PTRE3G promoter—mutations have reduced background expression

from the inducible promoter by up to 20-fold.

2. Tet-On 3G transactivator protein—mutations have significantly increased

its sensitivity to the inducer doxycycline (Dox).

Visit www.clontech.com/teton3g to learn more.

Cat. # Product Package Size

631168 Tet-On 3G Inducible Expression System Each

631167 Tet-On 3G Inducible Expression System (EF1α Version) Each

631166 Tet-On 3G Inducible Expression System (Bicistronic Version) Each

631165 Tet-On 3G Inducible Expression System (with mCherry) Each

631164 Tet-On 3G Inducible Expression System (with ZsGreen1) Each

631107631106

Tet System Approved FBS 50 ml500 ml

631311 Doxycycline 5 μg

631131631132

TetR Monoclonal Antibody (Clone 9G9) 40 μg200 μg

Notice to PurchaserYour use of these products and technologies is subject to compliance with any applicable licensing requirements described on the product’s web page at http://www.clontech.com. It is your responsibility to review, understand and adhere to any restrictions imposed by such statements.

Clontech® products are to be used for research purposes only. They may not be used for any other purpose, including, but not limited to, use in drugs, in vitro diagnostic purposes, therapeutics, or in humans. Clontech products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without prior written approval of Clontech Laboratories, Inc.

The Tet-On 3G Systems allow inducible gene expression only in the presence of doxycycline.

No Dox Dox

Transcription

Doxycycline

Tet-On 3GTransactivator

PTRE3GPromoter

www.clontech.com

6.13 IN (633209)

631107631106

631131631132

No Dox Dox

Transcription

Doxycycline

PTRE3GPromoter

1 0 % ~ 3 5 % O F F S A L E

www.clontech.com

639543 24 rxns 196,000

639542 96 rxns 495,000

639546 24 rxns 196,000

639545 96 rxns 495,000

639549 24 rxns 196,000

639548 96 rxns 495,000

Tet-On 3G Inducible Expression System 631168 each 1,625,000

Lenti-X Tet-On 3G Inducible Expression System 631187 each 2,894,000

Adeno-X Adenoviral System 3 (Tet-On 3G Inducible) 631180 each 3,057,000

Retro-X Tet-On 3G Inducible Expression System 631188 each 2,615,000

Tet System Approved FBS, USDA-Approved 631106 500 ml 399,000

Tet System Approved FBS, US-Sourced 631101 500 ml 570,000

Doxycycline 631311 5 g 210,000

Tet-On 3G Inducible Expression Systems 할인

SMARTer PCR cDNA Synthesis & SMARTer RACE 시리즈 할인

1 0 % ~ 3 5 % O F F S A L E

www.clontech.com

639543 24 rxns 196,000

639542 96 rxns 495,000

639546 24 rxns 196,000

639545 96 rxns 495,000

639549 24 rxns 196,000

639548 96 rxns 495,000

1 0 % ~ 3 5 % O F F S A L E

www.clontech.com

639543 24 rxns 196,000

639542 96 rxns 495,000

639546 24 rxns 196,000

639545 96 rxns 495,000

639549 24 rxns 196,000

639548 96 rxns 495,000

전 품 목 1 0 ~ 2 0 % 할 인C L O N T E C H C A M P A I G N

Lentiviral Delivery를 위한 노하우 총집합 ~ 할인

EcoDry RNA to cDNA Premix 시리즈 할인높은 증폭효율, 편리성을 갖춘 동결건조 타입의 premix 역전사 효소

Lowest background, highest sensitivity, and highest induction

C l o n t e c h C a m p a i g n

www.takara.co.kr / m.takara.co.kr

)분 51(응반 noisuF-nI .2폭증 RCP .1

Vector 말단 서열의 15 bp를 목적 DNA 증폭용 primer에 첨가하여 설계

Insert의PCR 증폭

In-Fusion 효소

목적 Vector

형질전환

Vector의제한효소처리or PCR 증폭

목적 DNA와 vector를 각각 PCR 증폭할 때

vector 말단의 약 15 bp를 목적 DNA 증폭용

primer에 추가하여 제작하는 것이 핵심이다.

(vector의 선형화는 제한효소 처리 가능)

증폭한 목적 DNA와 선형화 vector를 혼합하고

In-Fusion enzyme을 첨가하여 반응한다.

Clontech 독자적인 In-Fusion 효소가 약 15염기의

상동 부분을 융합한다.

Subcloning 없이 vector에 directional cloning이

가능하다. 상동 서열 부분은 원래 vector에 있던

서열이므로, 추가 서열의 부가는 일어나지 않는다.

분 류 소효 RCP slleC 제정 량 용 edoC 명 품 제 캠페인가

Liquid Type

000,303 O O SN 회 01 909836 sulP gninolC DH noisuF-nI

000,303 O O EC 회 01 619836 EC sulP gninolC DH noisuF-nI

000,632 - O - 회 01 246936 slleC/w tiK gninolC DH noisuF-nI

000,632 - - SN 회 01 936936 SN/w tiK gninolC DH noisuF-nI

000,632 - - EC 회 01 336936 EC/w tiK gninolC DH noisuF-nI

000,691 - - - 회 01 846936 tiK gninolC DH noisuF-nI

EcoDry

000,981 - - - 회 8 986936 tiK gninolC yrDocE DH noisuF-nI

정제 : NS(NucleoSpin), CE(Cloning Enhancer) Cell : Stellar Competent Cell PCR 효소 : CloneAmp HiFi PCR Premix

CloneAmp HiFi PCR Premix(Code 639298)은 2x MasterMix제품으로 높은 정확도와 뛰어난 증폭효율을 지닌 high fidelity PCR효소로 In-Fusion Cloning에 사용하기에 적합하다.

제한효소 사이트 구애없이, subcloning없이 원하는 vector에 클로닝

In-Fusion HD Cloning 시리즈 20% 할인

Packaging

000,044,1 회 02 742136 metsyS gnigakcaP XTH X-itneL

System

44,1 회 02 152136 metsyS gnigakcaP ciportocE XTH X-itneL 0,000

Lenti-X HTX Packaging System (Integrase Deficient) 631258 20 회 1,434,000

000,684 lm 1 081236 eniL lleC T392 X-itneL

Titeration

000,333 회 02 342136 xitSoG X-itneL

00,675 회 002 932136 tiK noitatitnauQ surivorP X-itneL 0

000,085 회002 532136 tiK noitartiT RCP-TRq X-itneL

000,556 회 69 002236 tiK retiT dipaR 42p X-itneL

00,062 lm 001 132136 rotartnecnoC X-itneL noitartnecnoC 0

3 회 2 332136 tiK noitacifiruP ixaM X-itneL noitacifiruP 28,000

000,259 lm 1 752136 rotareleccA X-itneL noitcudsnarT

Lentivirus 제작을 위한 Full Line-up

Lenti-X

expression SystemPackaging System Titeration

Concentration

PurificationTransduction Provirus 분석

일반 vector, Tet-Inducible용, 형광단백질포함, EF1-α promoter 등 다양한 vector 보유

분류 Code 품목명 용량 캠페인가

Lentiviral Vector 다수 다양한 포맷의 Lentiviral vector 보유 10% 할인

Packaging

631247 Lenti-X HTX Packaging System 20 회 1,440,000

System

631251 Lenti-X Ecotropic Packaging System 20 회 1,440,000

631258 Lenti-X HTX Packaging System (Integrase Deficient) 20 회 1,434,000

632180 Lenti-X 293T Cell Line 1 ml 486,000

Titeration

631243 Lenti-X GoStix 20 회 333,000

631235 Lenti-X qRT-PCR Titration Kit 200 회 579,000

632200 Lenti-X p24 Rapid Titer Kit 96 회 654,000

Concentration 631231 Lenti-X Concentrator 100 ml 259,000

Purification 631233 Lenti-X Maxi Purification Kit 2 회 328,000

Transduction 631254 Lenti-X Accelerator Starter Kit each 1,104,000

Enhancer 631471 Ecotropic Receptor Booster 20 회 357,000

Provirus 분석 631239 Lenti-X Provirus Quantitation Kit 200 회 576,000

631263 Lenti-X Integration Site Analysis Kit each 1,132,000

분류 제품명 Code 용량 캠페인가

NGS SMARTer Ultra Low Input RNA for Illumina Sequencing-HV 634820 12 회 2,553,000

SMARTer Universal Low Input RNA Kit for Sequencing* 634938 10 회 2,208,000

cDNA SMARTer PCR cDNA Synthesis Kit 634925 10 회 1,247,000

합성 SMARTer Pico PCR cDNA Synthesis Kit 634928 10 회 1,942,000

RACE SMARTer RACE cDNA Amplication Kit 634923 10 회 1,209,000

Marathon cDNA Amplification Kit 634913 5 cDNA 1,191,000

SMART cDNA Library Construction Kit 634901 10 회 1,616,000

RNA to cDNA EcoDry Premix (Oligo dT)

639543 24 rxns 196,000

639541 48 rxns 291,000

639542 96 rxns 494,000

RNA to cDNA EcoDry Premix (Random Hexamers)

639546 24 rxns 196,000

639544 48 rxns 291,000

639545 96 rxns 494,000

RNA to cDNA EcoDry Premix (Double Primed*)

639549 24 rxns 196,000

639547 48 rxns 291,000

639548 96 rxns 494,000

www.clontech.com

1212 US (633057)

United States/Canada: +1.800.662.2566 • Asia Pacific: +1.650.919.7300 • Europe: +33.(0)1.3904.6880 • Japan: +81.(0)77.543.724For Research Use Only. Not for use in diagnostic or therapeutic procedures. Not for resale. Clontech®, the Clontech logo, Advantage, EcoDry, SMART, and Titanium are trademarks of Clontech Laboratories, Inc. All other marks are the property of their respective owners. Certain trademarks may not be registered in all jurisdictions.©2012 Clontech Laboratories, Inc.

P C R , R T - P C R , & R E A L - T I M E q P C R

RNA to cDNA EcoDry Premix

Our RNA to cDNA EcoDry Premix allows first-strand cDNA synthesis with the

convenience and flexibility of the EcoDry format.

• Add RNA and go!

• Features our high-yield SMART™ MMLV Reverse Transcriptase (RT)

• Synthesize high-quality, full-length cDNA from virtually any transcript

• Each tube of master mix includes SMART MMLV RT, dNTPs, and your

choice of oligo(dT)18 primers, random hexamer primers, or a combination

of both

Benefits of EcoDry Premixes

• Pre-aliquoted mixes are ready-to-use

• Single-use tubes minimize contamination

• Simple protocol with minimal hands-on time

• Features our high-performance enzymes

• Convenient room-temperature storage

• Flexible formats - single to 96 reactions

EcoDry lyophilized master mixes are stable at room temperature, allowing DNA amplifica-tion and first-strand cDNA synthesis with minimal effort and maximum ease. This convenient format also minimizes waste in storage and shipping, making it the eco-friendly choice.

EcoDry premixes are packaged in 8-well tube strips with clear sealing caps.

EcoDry™ PCR & Reverse Transcription PremixesEasy, Convenient, and Foolproof RT and PCR

Eco-friendly Single-use Ready-to-go Robust

* Oligo dT와 Random Hexamers가 동시에 포함되어 있다.

* 리스트 외에 다양한 format의 제품 보유 (자세한 리스트는 홈페이지 참조)

* Random primer를 사용하여 non-polyA RNA(bacteria RNA) sample 적용 가능

SMARTer cDNA synthesis occurs in a singlestep reverse transcription reaction. Following

amplification, SMARTer cDNA can be used for a variety of downstream applications.

First-strandcDNA synthesis

SMARTeroligo

Oligo(dT) primerwith added

PCRadaptor

poly A

5' poly A

SMARTer cDNA Synthesis

LD-PCR amplificationor primer extension

Second-strandcDNA synthesis

Full-lengthds cDNA

Startingmaterial

Singlestep

Templatefor real-time

Next-generation

Direct geneamplification

Libraryconstruction

SMARTScribe MMLV RT

obesfor arrays

Subtractivehybridization

NA the SMARTer™ Way

NA from as little as 1–2 ng of total RNAles and maintain accurate gene representation

wer background, and increased yieldNA

necessarynger genes and rare transcripts

MARTer chnology

MARTer RACE cDNA Ampli�cation Kit

www.clontech.com

6.13 IN (633209)

631107631106

631131631132

No Dox Dox

Transcription

Doxycycline

PTRE3GPromoter

www.clontech.com

6.13 IN (633209)

631107631106

631131631132

No Dox Dox

Transcription

Doxycycline

PTRE3GPromoter

1 0 % ~ 3 5 % O F F S A L E

www.clontech.com

639543 24 rxns 196,000

639542 96 rxns 495,000

639546 24 rxns 196,000

639545 96 rxns 495,000

639549 24 rxns 196,000

639548 96 rxns 495,000

Tet-On 3G Inducible Expression System 631168 each 1,625,000

Lenti-X Tet-On 3G Inducible Expression System 631187 each 2,894,000

Adeno-X Adenoviral System 3 (Tet-On 3G Inducible) 631180 each 3,057,000

Retro-X Tet-On 3G Inducible Expression System 631188 each 2,615,000

Tet System Approved FBS, USDA-Approved 631106 500 ml 399,000

Tet System Approved FBS, US-Sourced 631101 500 ml 570,000

Doxycycline 631311 5 g 210,000

Tet-On 3G Inducible Expression Systems 할인

SMARTer PCR cDNA Synthesis & SMARTer RACE 시리즈 할인

1 0 % ~ 3 5 % O F F S A L E

www.clontech.com

639543 24 rxns 196,000

639542 96 rxns 495,000

639546 24 rxns 196,000

639545 96 rxns 495,000

639549 24 rxns 196,000

639548 96 rxns 495,000

2012 HOT I TEM 20% Clontech 전품목 10 ~ 20% 할인 전제품...

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Transcript of 2012 HOT I TEM 20% Clontech 전품목 10 ~ 20% 할인 전제품...