Proceedings 17-18 May 2010

Shanhua, Taiwan

2010 APSA - AVRDC Workshop

Proceedings of the 2010

APSA - AVRDC WORKSHOP

17-18 May 2010

AVRDC - The World Vegetable Center

Shanhua, Tainan

Taiwan

AVRDC - The World Vegetable Center is the leading international nonprofit research and development institute committed to alleviating poverty and malnutrition in the developing world through the increased production and consumption of nutritious, health-promoting vegetables.

Published by AVRDC - The World Vegetable Center P.O. Box 42 Shanhua, Tainan 74199 TAIWAN Tel: +886 6 583 7801 Fax: +886 6 583 0009

Email: info@worldveg.org Web: www.avrdc.org

AVRDC Publication: 10-741 ISBN 92-9058-183-2

Editor: Maureen Mecozzi Photos: Chen Ming-che Minutes: Warwick Easdown

This work is licensed under the Creative Commons Attribution-ShareAlike 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-sa/3.0/tw/ or send a letter to Creative Commons, 171 Second Street, Suite 300, San Francisco, California, 94105, USA.

Suggested citation AVRDC - The World Vegetable Center. 2010. Proceedings of the APSA- AVRDC Workshop. 17-18 May 2010. AVRDC - The World Vegetable Center, Shanhua, Taiwan. AVRDC Publication 10-741. 41 p.

Proceedings of the 2010 APSA - AVRDC WORKSHOP

CONTENTS

Executive Summary 5

Program 7

Minutes 9

Impressions 29

Participants - APSA 32

Participants - AVRDC 38

Acronyms 40

Appendix: CD ROM

5AVRDC - The World Vegetable Center

Executive summary The fourth joint workshop between AVRDC – The World Vegetable Center (AVRDC) and the Asia and Pacific Seed Association (APSA) held on 17-18 May 2010 was a time to evaluate the status of the working relationship and chart a new course for future collaboration. Twenty-six APSA members, 28 AVRDC staff, and Victoria Henson Apollonio and Guat Hong Teh, observers from the CGIAR Central Advisory Service on Intellectual Property (CAS-IP), participated in the meeting at the Center’s Headquarters in Shanhua, Taiwan. Jackie Hughes, AVRDC’s Deputy Director General for Research and Anthony Tse of the Clover Seed Co. Ltd., welcomed seed company owners, managers and scientists from Thailand, Philippines, India, China, Taiwan, and Japan to discuss issues of mutual interest with AVRDC’s management and scientists.

Presentations and discussions were followed by a field tour. Twelve scientists presented the Center’s recent work in vegetable breeding, germplasm, biotechnology,

gene discovery, nutrition, and plant protection. Some highlights:

• Peter Hanson discussed the incorporation of marker- assisted selection (MAS) into the AVRDC tomato breeding program to develop tomatoes resistant to biotic stress. He noted a recent comparison of costs indicated MAS was slightly more expensive than conventional means, but can save a lot of effort in transplanting, provides much more information, and can improve the efficiency of selections.

• Andreas Ebert presented a review of the AVRDC genebank—the world’s largest public-sector collection of vegetable germplasm, with more than 57,000 accessions from 408 countries. He emphasized the need to collect data on abiotic stress from many locations, to give more full characterization to existing lines in the collection.

• Edwin Javier introduced genotype-environment analysis of AVRDC’s International Chili Pepper Nursery. The international nurseries provide a set of improved lines for evaluation in different countries and environments.

Proceedings of the 2010 APSA-AVRDC Workshop6

• Nutritionist Ray-yu Yang gave an overview of global nutritional status, and discussed the Center’s work in developing vegetable lines with improved nutritional qualities. Breeding for nutritional content has produced tomatoes with high levels of beta-carotene, providing 3 to 9 times the vitamin A of normal tomatoes, and heat-tolerant peppers with high carotenoid and very high vitamin C content.

• In her presentation on the development of tomato simple sequence repeat markers and their use in mapping bacterial wilt resistance, Jaw-fen Wang explained why the markers are becoming indispensable to plant pathologists: the markers are highly reproducible, inexpensive, and simple to handle; they can be used by small labs; and can differentiate homozygous and heterozygous lines.

All twelve presentations were followed by question-and- answer sessions.

On the field tour organized by the AVRDC Virology team, participants visited a preliminary yield trial of multiple TY tomato lines and the International Sweet and Chili Pepper Nurseries. An evaluation trial of leafy Brassicas and a cucumber yield

trial were also toured. Tomato differentials to Phytophthora infestans races were on display in the glasshouses, and participants received a begomovirus resistance screening demonstration in the virology screenhouse.

As always, the Center will ship seed from its collection at the request of any APSA member. APSA can help the Center’s genebank expand further by sharing lines with interesting horticultural characteristics with our Genetic Resources and Seed Unit. The Center encourages all APSA members to acknowledge the use of AVRDC germplasm in their catalogs.

High-quality vegetables that fetch good prices in domestic and export markets—and improve incomes and nutrition for small-scale farmers—begin with quality seed. Both AVRDC and APSA will seek to increase farmers’ access to improved vegetable seed in developing and developed countries alike by building on the strong partnership that has been forged during the past four years.

7AVRDC - The World Vegetable Center

Program

Monday 17 May 2010 Presentation Presenter

0830-0900 Registration

0900-0920 Introduction and AVRDC’s global research and development agenda

Jackie Hughes

0920-0930 Remarks Anthony Tse (for APSA)

Vegetable Breeding, Germplasm

Chair: Willy Co 0930-1010 Application of molecular markers in tomato

breeding for resistance to biotic stresses Peter Hanson

1010-1040 Group photo and coffee break

1040-1120 Progress in breeding heat tolerant sweet pepper and pepper program update

Paul Gniffke

1120-1200 Plant genetic resources and vegetable breeding

Andreas Ebert

1200-1315 Lunch

1315-1345 Progress of AVRDC cucurbit breeding Narinder Dhillon (presented by Paul Gniffke)

1345-1400 AVRDC on-line seed catalogue Edwin Javier

1400-1430 Genotype-environment analysis of International Chili Pepper Nursery

Edwin Javier

1430-1500 General discussion: breeding

1500-1530 Coffee break

Biotechnology, Gene Discovery, Nutrition

Chair: Surinder Tikoo 1530-1600 Vegetables: phytonutrients, health

benefits, and value addition Ray-yu Yang

1600-1620 Development of tomato SSR markers from genomic sequences of BAC clones on chromosome 6 and their use in mapping bacterial wilt resistance

Jaw-fen Wang

1620-1650 AVRDC research on abiotic stress tolerance in tomato

Kadirvel Palchamy

1650-1700 Discussion: biotechnology, gene discovery, nutrition

1845-2030 Welcome dinner, AVRDC Cafeteria

Proceedings of the 2010 APSA-AVRDC Workshop8

Tuesday 18 May 2010 Field tour

Coordinator: Lawrence Kenyon 0820 Board bus in front of Administration Building

0830–0905 Field 60: Preliminary yield trial of multiple TY tomato lines (Peter Hanson)

0910–0940 Field 101: International Sweet and Chili Pepper Nurseries (Paul Gniffke)

0945–1005 Field 34: Leafy Brassica Evaluation Trial (Ruby Hsiao)

1010–1030 Refreshment Break (Tent and chairs near Field 46)

1035–1100 Field 46: Cucumber yield trial (Chung-cheng Lin)

1105–1125 Old Glasshouse: Tomato differentials to Phytophthora infestans races (Jaw-Fen Wang)

1130–1150 Virology screenhouse: Begomovirus resistance screening and symptoms of different cucurbit viruses (Wen-Shi Tsai)

1200-1300 Lunch

Plant Protection Presenter

Chair: Winnie Liu

1300-1330 Overview of AVRDC virology research Lawrence Kenyon

1330-1400 Developing insect and mite resistant vegetable varieties: status and opportunities at AVRDC

Srinivasan Ramasamy

1400-1420 Reactions of Phytophthora infestans isolates on tomato accessions with late blight resistance

Jaw-fen Wang

1420-1500 Coffee break

1500-1530 Discussion

1530-1600 General Wrap-up discussion Jackie Hughes

1830-2100 Farewell dinner, AVRDC Cafeteria

9AVRDC - The World Vegetable Center

Minutes DAY 1 Wednesday 17 May 2010

Introduction and AVRDC’s global research and development agenda Jackie Hughes

You are all very welcome here. Mr. Anthony Tse will be making remarks on behalf of APSA as Dr. Jaswant Singh unfortunately was unable to attend. We also welcome Victoria Henson-Apollonio and Guat Hong Teh from the CGIAR Central Advisory Service for Intellectual Property as observers. We see this meeting as a wrap up of our work with APSA over the la