2006-2007 Movement across the Cell Membrane. Diffusion Diffusion movement from HIGH → LOW...

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2006-2007 Movement across the Cell Membrane

Transcript of 2006-2007 Movement across the Cell Membrane. Diffusion Diffusion movement from HIGH → LOW...

Page 1: 2006-2007 Movement across the Cell Membrane. Diffusion  Diffusion  movement from HIGH → LOW concentration  Down the concentration gradient  Diffusion.

2006-2007

Movement across the Cell Membrane

Page 2: 2006-2007 Movement across the Cell Membrane. Diffusion  Diffusion  movement from HIGH → LOW concentration  Down the concentration gradient  Diffusion.

Diffusion

DiffusionDiffusionmovement from HIGH → LOW concentrationDown the concentration gradient

DiffusionDiffusionmovement from HIGH → LOW concentrationDown the concentration gradient

Is this a passive or

active process?

Is this a passive or

active process?

What did it take to What did it take to make the make the

concentration concentration gradient?gradient?

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32006-2007

The concentration gradient stores potential energy

It takes energy to generate the gradient

The process is still considered passive

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Facilitated Diffusion• Diffusion through

protein channels– highly specific– passive process

“The Bouncer”

HIGHHIGH

LOWLOW

what sort of what sort of molecules molecules

will use this will use this process?process?

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Active Transport

“The Doorman”

• Cells may need to move molecules against concentration gradient– conformational shape

change transports solute from one side of membrane to other

– protein “pump”– “costs” energy = ATP

ATP

LOWLOW

HIGHHIGH

Once completed, Once completed, where has the where has the energy for the energy for the

active transport active transport gone? gone?

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symportsymportantiportantiport

Active transport• Many models & mechanisms

ATP ATP

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Getting through cell membrane• Passive Transport

– Simple diffusion• nonpolar, hydrophobic molecules

–HIGH → LOW concentration gradient– Facilitated transport

• polar, hydrophilic molecules• through a protein channel

–HIGH → LOW concentration gradient• Active transport

– against concentration gradient• LOW → HIGH

– uses a protein pump (requires ATP)ATP

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Transport summary

simplesimplediffusiondiffusion

facilitatedfacilitateddiffusiondiffusion

activeactivetransporttransport

ATP

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What about large molecules?

• Moving large molecules into & out of cell– through vesicles & vacuoles– endocytosis

• phagocytosis = “cellular eating”• pinocytosis = “cellular drinking”

– exocytosis

exocytosis

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Endocytosis

phagocytosis

pinocytosis

receptor-mediated endocytosis

fuse with lysosome for digestion

non-specificprocess

triggered bymolecular signal

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What about Osmosis?

• Diffusion of water from HIGH concentration of water to LOW concentration of water– across a

semi-permeable membrane

Why does Why does osmosis need osmosis need a membrane?a membrane?

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Concentration of water

• Direction of osmosis is determined by comparing total solute concentrations– Hypertonic - more solute, less water

– Hypotonic - less solute, more water

– Isotonic - equal solute, equal water

hypotonic hypertonic

water

net movement of water

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Hypotonic , Hypertonic, Isotonic

How does sugar How does sugar concentration lead to concentration lead to water movement? water movement?

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Which way would Which way would water move if oil was water move if oil was added to the left side added to the left side

of the tube?of the tube?

Let’s watch the video

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Managing water balance

• Hypotonic• Animal cell/protist

cell problem?

• Solutions?

• Plant cells....

• turgid = full• cell wall protects

from bursting

freshwater

No problem,here

KABOOM!

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Managing water balance• Hypertonic

• Animal cells:• How can they adjust:• Take up water or pump

out solutes.– plant cells

• plasmolysis = wilt

saltwater

I willsurvive!

I’m shrinking,I’m shrinking!

Why do people make Why do people make beef jerky, and add beef jerky, and add syrup to preserve syrup to preserve

canned fruit?canned fruit?

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Managing water balance• Isotonic

–Animal cell problems:–none–solutes are equally

concentrated on both sides of membranes

–Plant cell problems–flaccid cells lose structure.

balanced

I couldbe better…

That’sperfect!

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Aquaporins• Water moves rapidly into & out of cells

– evidence that there were water channels• protein channels allowing flow of water across

cell membrane

Peter AgreJohn Hopkins

Roderick MacKinnonRockefeller

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.05 M.05 M .03 M.03 M

Which way will water Which way will water

move?move?