Sam S. Yoon, Neil H. Segal, Peter J. Park, Kara Y. Detwiller,
2004. 5. 25 Ji-Yoon Park
15
2004. 5. 25 Ji-Yoon Park Nanoparticle-Based Theorem Proving
description
Nanoparticle-Based Theorem Proving. 2004. 5. 25 Ji-Yoon Park. Target Problem. P ∧Q→R, S∧T→Q, S, T, P R = True ?. DNA-Based Nanoparticle Assembly Strategy. Mirkin, 2000. Experimental Section. 1. Purchase of gold nanoparticle. - PowerPoint PPT Presentation
Transcript of 2004. 5. 25 Ji-Yoon Park
2004. 5. 25
Ji-Yoon Park
Nanoparticle-Based Theorem Proving
May 25, 2004 May 25, 2004 APRL http://gene.hanyang.ac.krAPRL http://gene.hanyang.ac.kr 22
Target Problem
P∧Q→R, S∧T→Q, S, T, P
R = True ?
May 25, 2004 May 25, 2004 APRL http://gene.hanyang.ac.krAPRL http://gene.hanyang.ac.kr 33
DNA-Based Nanoparticle Assembly Strategy
Mirkin, 2000Mirkin, 2000
May 25, 2004 May 25, 2004 APRL http://gene.hanyang.ac.krAPRL http://gene.hanyang.ac.kr 44
1. Purchase of gold nanoparticle1. Purchase of gold nanoparticle
2. Preparation of 3’-(alkanethiol) modified oligonucleotides
2. Preparation of 3’-(alkanethiol) modified oligonucleotides
3. Preparation of Linker DNA 3. Preparation of Linker DNA
4. Characterization of DNA-linked gold assembly 4. Characterization of DNA-linked gold assembly
Experimental Section
May 25, 2004 May 25, 2004 APRL http://gene.hanyang.ac.krAPRL http://gene.hanyang.ac.kr 55
Primer Sequence(5’→3’) Length Modification
ST¬QAAG CAG TAG CGA CCA ATT GAC GCAAAT TGA CGT ACG TAC GCT GAA 45 mer None
¬RPQCAT ACA ATG AAC GCA GTC AAC GCA AGG CAG TTC AGC GTA CGT ACG 45 mer None
¬P CTG CCT TGC GTT GAC 15 mer 5’-phosphate
R TGC GTT CAT TGT ATG 15 mer 3’-thiol
¬S TGG TCG CTA CTT 15 mer 3’-thiol
¬T TCA ATT TGC GTC AAT 15 mer 5’-phosphate
The Sequence of Oligonucleotides
May 25, 2004 May 25, 2004 APRL http://gene.hanyang.ac.krAPRL http://gene.hanyang.ac.kr 66
Assembly
15 mer15 mer 15 mer15 mer 45 mer45 mer
S1 Digestion
Hypothesis
May 25, 2004 May 25, 2004 APRL http://gene.hanyang.ac.krAPRL http://gene.hanyang.ac.kr 77
Colloidal Gold
May 25, 2004 May 25, 2004 APRL http://gene.hanyang.ac.krAPRL http://gene.hanyang.ac.kr 88
Full Assembly vs S1 Digestion
May 25, 2004 May 25, 2004 APRL http://gene.hanyang.ac.krAPRL http://gene.hanyang.ac.kr 99
Partial Assembly vs S1 Digestion
May 25, 2004 May 25, 2004 APRL http://gene.hanyang.ac.krAPRL http://gene.hanyang.ac.kr 1010
TEM Image (Colloidal Gold)
(a)(a) (b)(b) (c)(c)
TEM image of the annealed DNA-linked assembly (a) Colloidal gold (b) Full-assembly of DNA-linked nanoparticles (c) S1 Nuclease digestion of full-assembled DNA-linked nanoparticles
Surface-Based Theorem Proving
May 25, 2004 May 25, 2004 APRL http://gene.hanyang.ac.krAPRL http://gene.hanyang.ac.kr 1212
Surface-based DNA Computation
Liu et al., 2000Liu et al., 2000
May 25, 2004 May 25, 2004 APRL http://gene.hanyang.ac.krAPRL http://gene.hanyang.ac.kr 1313
1. Purchase of gold nanoparticle1. Purchase of gold nanoparticle
2. Preparation of 3’-(alkanethiol) modified oligonucleotides
2. Preparation of 3’-(alkanethiol) modified oligonucleotides
3. Preparation of Linker DNA 3. Preparation of Linker DNA
4. Characterization of DNA-linked gold assembly 4. Characterization of DNA-linked gold assembly
Experimental Section
May 25, 2004 May 25, 2004 APRL http://gene.hanyang.ac.krAPRL http://gene.hanyang.ac.kr 1414
I
II
III
IV
V
A B C
(a) (b) I
II
III
IV
V
I
II
III
IV
V
A B C
A B C
(c)
¬RPQ¬RPQ¬RPQ
¬T¬T¬T
¬S¬S¬S
P.CP.C P.C
N.CN.C N.C
R¬PST ¬Q
¬RPQ¬RPQ¬RPQ
¬T¬T¬T
¬S¬S¬S
P.CP.C P.C
N.CN.C N.C
Theorem Proving by Fluorescence Imaging
May 25, 2004 May 25, 2004 APRL http://gene.hanyang.ac.krAPRL http://gene.hanyang.ac.kr 1515
• Paper publish • Genotyping experiment• Structure & Theorem Proving
Further Work
