12th International Symposium, Exhibit and Workshops on...

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12th International Symposium, Exhibit and Workshops on

Preparative / Process Chromatography,

Ion Exchange,

Adsorption / Desorption Processes

& Related Separation Techniques

May 23 – 26, 1999 Holiday Inn Golden Gateway

San Francisco, CA, USA

Final Program

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1999 PREP SYMPOSIUM & EXHIBIT

SYMPOSIUM CHAIRMEN

DR. GEORGES GUIOCHON DR. JOHN FRENZ

University of Tennessee and Genentech

Oak Ridge National Laboratory

ORGANIZING COMMITTEE

Firoz Antia, Merck & Company

Giorgio Carta, University of Virginia

John Frenz, Genentech

Georges Guiochon, Univ. of Tennessee and Oak Ridge National Laboratory

Jana Jacobson, Lygra-mura

Anita Katti, Engineering and Process Chromatography Solutions

Klaus Lohse, BTR-Separations

Joan Newburger, R.W. Johnson Pharmaceutical Research Institute

SPONSORS

We would like to acknowledge the following organizations for their support:

NovaSep, Prochrom, TechniKrom, and Waters

SYMPOSIUM MANAGER

Ms. Janet Cunningham

Barr Enterprises

P. O. Box 279

Walkersville, MD 21793 USA

Phone 301-898-3772 Fax 301-898-5596 E-mail [email protected]

mailto:[email protected]

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GENERAL INFORMATION

BADGES A name badge must be worn by each registered participant and

accompanying persons in order to gain admittance to the meeting, exhibits,

posters, and social gatherings.

REGISTRATION If you need assistance, please come to the Symposium Registration

Desk located outside the Emerald Room on the lobby level. The

Registration Desk will be open throughout the Symposium.

PROGRAM Lectures will be held in the Emerald Room. On the lower level, posters

will be displayed in the Pacific Suite Rooms, Exhibits will be held in the

Gold Rush Ballroom, and receptions and coffee breaks will be located

near the Exhibit area.

POSTER — Poster boards are labeled with the number corresponding to the

SESSIONS abstract number in the Final Program Book.

— Authors presenting posters are requested to be in attendance at their

posters during the times indicated in the program.

— Posters with program numbers in the 100 series will be presented on

Monday, and posters with program numbers in the 200 series will be

presented on Tuesday.

— Poster Sessions will take place in the Pacific Suite

— All posters should be mounted on Monday between 8AM and 1PM,

and will remain on display all day Monday and Tuesday so attendees

have opportunities to view all posters throughout both days.

— Posters must be removed by 6PM on Tuesday (anything remaining

after that time will be discarded).

AUTHOR INDEX Author Index is located at the end of the Final Program Book

“L” preceding abstract number = lecture

“P” preceding abstract number = poster

EXHIBITS The exhibition is an important component of the meeting, so please

take time to thank all the exhibitors for their support of the program.

Exhibits will be held in the Gold Rush Ballroom located on the lower level

of the hotel. Exhibit hours are Monday and Tuesday, 8:30 am – 7:00 pm.

Exhibit-only guest passes are available on Tuesday, May 25, from 9:00 am

to 7:00 pm.

SPEAKER For those who will give lecture presentations and wish to

PREVIEW ROOM preview their slides, they may do so in the Executive

Conference Room, located across from the Emerald Room.

SPONSORS Monday 6-7PM Reception sponsored by TECHNIKROM

Tuesday 6-7PM Reception sponsored by PROCHROM

Monday coffee break sponsored by NOVASEP

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CORPORATE SPONSORS

We would like to acknowledge the following organizations for their support:

TECHNIKROM

PROCHROM

WATERS

NOVASEP

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FREE VENDOR WORKSHOPS

• To attend, Symposium registrants MUST PRE-REGISTER (see details below)

• Booths are located in the Gold Rush Ballroom located on the lower level of the hotel

• Space is limited and pre-registration is required

MONDAY, MAY 24

12:30 – 1:45 pm Workshop on Practical Issues in the Ion Exchange

Chromatography of Proteins at Production Scale

Sponsored by Whatman — complimentary lunch provided

Location: Emerald Room (lobby level of hotel)

Pre-registration at Whatman’s booth is required

TUESDAY, MAY 25

7:15 – 8:30 am Workshop on BioProcessing Equipment Design Considerations

Sponsored by TechniKrom — complimentary continental breakfast provided


Pre-registration at TechniKrom’s booth is required

— OR —

7:15 – 8:30 am Workshop on BioPharmaceutical Purification Processes

Sponsored by Sepragen — complimentary continental breakfast provided

Location: Monterey/Carmel Room (lower level of hotel)

Pre-registration at Symposium Registration Desk is required

TUESDAY, MAY 25

12:30 – 1:45 pm Workshop on Gradiflow—A Revolution in Protein Purification

Sponsored by Gradipore — complimentary lunch provided


Pre-registration at Gradipore’s booth is required

— OR —

12:30 – 1:45 pm Workshop on Recent Advances in Recycling Preparative HPLC

Sponsored by DyChrom — complimentary lunch provided



WEDNESDAY, MAY 26

7:15 – 8:30 am Workshop on An Introduction to EasyPack and Geltech

Industrial Chromatography Columns – easy to use, sanitary design

columns for pilot and production scale biotherapeutic manufacturing

Sponsored by PE Biosystems — complimentary continental breakfast provided


Pre-registration at PE Biosystems’ booth is required

— OR —

7:15 – 8:30 am Workshop on Optimization in Industrial HPLC

Sponsored by EKA Nobel — complimentary continental breakfast provided

Location: Crystal Room (lobby level of hotel)

Pre-registration at BTR-Separations’ booth is required

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MONDAY, MAY 24

— Meeting Location: Emerald Room —

8:00 am Registration

8:30 am Exhibits Open in Gold Rush Ballroom (location of coffee breaks)

8:55 am Opening Remarks

CHAIR: DR. JOHN FRENZ

9:00 am (L-101) The Challenge for HPLC Separations in the Post-Genome Era

—William S. Hancock, J. Chakel, A. Apffel, K. Hahnenberger,

G. Choudhray, Hewlett-Packard, Palo Alto, CA, USA; E. Lehmberg,

E. Pungor, Jr., Berlex Biosciences, Richmond, CA, USA;

Cs. Horvath, Yale University, New Haven, CT, USA

9:20 am (L-102) Optimization of the Extraction and Purification of a

Recombinant Protein Pharmaceutical—Raymond Arnold,

J. Cacia, J. Frenz, Genentech, S. San Francisco, CA, USA

9:40 am (L-103) Purification of Recombinant Proteins From Yeast Broth

Containing a High Percentage of Solids Using Expanded Bed

Chromatography—Shiaw-Lin (Billy) Wu, R. Caren, I. Vassilieva,

F. Leung, P. Stathis, S. Chamow, Scios, Mountain View, CA, USA

10:00 am (L-104) Process Validation in the Development of the Recombinant

Protein Pharmaceutical Lenercept—Marjorie E. Winkler,

M. Field, Z. Shahrokh, M. Vanderlaan, Genentech, S. San

Francisco, CA, USA

10:20 am BREAK sponsored by NOVASEP

Location: Gold Rush Ballroom on lower level of hotel

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MONDAY, MAY 24


CHAIR: PROF. GIORGIO CARTA

10:50 am (L-105) Preparative Purification and Quality Control of Biologically

Active Proteins From Human Plasma—Djuro Josic, H. Schwinn,

A. Buchacher, P. Schulz, G. Gruber, K. Pock, Octapharma

Pharmazeutika Produktionsges. m.b.h., Vienna, AUSTRIA

11:10 am (L-106) Integrating Maldi-TOF and Preparative-LC as a Tool in the

Identification of Recombinant Proteins as Vaccine Candidates—

Michael Meys*, A. Sultana†, R.G. Ulrich†, P. Lynch*, T. Nadler*,

D. Whitney*, and D. Patterson*, PE Biosystems, Framingham, MA,

USA; †USAMRIID, 1425 Porter St., Fort Detrick, Frederick, MD,

USA

11:30 am (L-107) Semi-Preparative Scale Purification of Monoclonal

Antibodies—Gerald Bell, N. Fuschetto, T. John, and S. Mische,

Boehringer Ingelheim Pharmaceuticals, Ridgefield, CT, USA

11:50 am (L-108) The Development and Optimization of Non-Affinity and

Non-Traditional Affinity Methods for Monoclonal Antibody

Purification—Natalie Fuschetto, G. Bell, T. John, S. Mische,

Boehringer Ingelheim Pharmaceuticals, Ridgefield, CT, USA

12:10-3:30 pm BREAK / EXHIBITS / POSTERS

12:30-1:45 pm WORKSHOP on Practical Issues in the Ion Exchange

Chromatography of Proteins at Production Scale

Sponsored by Whatman—complimentary lunch provided


Pre-registration at Whatman’s booth is required

2:00-3:15 pm POSTER SESSION

Location: Pacific Suite (lower level of hotel)

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MONDAY, MAY 24


CHAIR: PROF. ATHANASIOS I. LIAPIS

3:30 pm (L-109-new) SMB Design for Chiral Separation: Optimal Particle Size of the

(moved from L-302) Stationary Phase—O. Ludemann-Hombourger, Roger M. Nicoud,

NovaSep SA, Vandoeuvre l’Nancy, FRANCE

3:50 pm (L-110) Highly-Productive Preparative Enantiomeric Separations Using

Carbon Dioxide Chromatography—F. Geiser, Mohamed

Shaima, J. K. Lee, Chiral Technologies, Exton, PA, USA

4:10 pm (L-111) Optimization of Chiral Separations Using Laser-Based

Polarmetric Detection—Gary W. Yanik*, D.R. Bobbitt, *PDR-

Chiral, Palm Beach Gardens, FL, USA; University of Arkansas,

Fayetteville, AR, USA

4:30 pm (L-112) The Preparative Separation of Racemic Mixtures:

A Comparison of the Conventional Recycling and Steady State

Recycling Techniques—Charles M. Grill*, L.A. Dolak, E.P.

Seest, *R&S Technology, Wakefield, RI, USA; Pharmacia &

Upjohn, Kalamazoo, MI, USA

4:50 pm PAUSE

5:10 pm GENERAL DISCUSSION

6:00-7:00 pm RECEPTION sponsored by TECHNIKROM


Visit the TechniKrom booth to receive complimentary drink tickets

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TUESDAY, MAY 25


7:15-8:30 am WORKSHOP on BioProcessing Equipment Design Considerations

Sponsored by TechniKrom—complimentary continental breakfast provided


Pre-registration at TechniKrom’s booth is required

7:15 – 8:30 am WORKSHOP on BioPharmaceutical Purification Processes

Sponsored by Sepragen — complimentary continental breakfast provided



8:30 am Exhibits Open in Gold Rush Ballroom (location of coffee breaks)

CHAIR: DR. ANDREAS SEIDEL-MORGENSTERN

9:00 am (L-201) Flow Distribution in Chromatographic Column—

Q. Sarah Yuan, J.S. Moscariello, M.A. Teeters, T.W. Root,

E.N. Lightfoot, University of Wisconsin, Madison, WI, USA

9:20 am (L-202) Evidence of Wall Friction in the Consolidation of Beds of

Packing Materials in Chromatographic Columns—

Georges Guiochon, E. Drumm, B.G. Yew, D. Cherrak, University

of Tennessee, Knoxville, TN, USA and Oak Ridge National

Laboratory, Oak Ridge, TN, USA

9:40 am (L-203) Network Modeling and Simulation of the Performance of

Continuous Bed Chromatography—J.J. Meyers,

Athanasios I. Liapis, University of Missouri, Rolla, MO, USA

10:00 am (L-204) Expanded-Bed Chromatography in Primary Protein

Purfication—F. Birger Anspach, GBF-Gesellschaft fuer

Biotechnologische Forschung mbH, Braunschweig, GERMANY

10:20 am BREAK


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TUESDAY, MAY 25


CHAIR: DR. ANTIA M. KATTI

10:50 am (L-205) Preparative Chromatography in Synthesis for Pharmaceutical

Market Registration: Regulatory Implications and Control

Strategies—Joan Newburger, R.W. Johnson Pharmaceutical

Research Institute, Spring House, PA, USA

11:10 am (L-206) Quantification of Single Solute and Competitive Adsorption

Isotherms Using a Closed-Loop Perturbation Method—

C. Bluemel¹, P. Hugo2, Andreas Seidel-Morgenstern3,

¹Wissenschaftlicher Gerätebau H. Knauer GmbH, Berlin,

GERMANY; 2Technische Universität Berlin, Berlin, GERMANY; 3Otto-von-Guericke Universität Magdeburg, Magdeburg,

GERMANY

11:30 am (L-207) An Investigation of Hydrophobic Interaction Chromatography

Using Flow Microcalorimetry—M. Esquibel-King, A.C. D.

Cabral*, J.A.S.R. Queiroz*, Neville G. Pinto, University of

Cincinnati, Cincinnati, OH, USA; *Universidade da Beira Interior,

Covilha, PORTUGAL

11:50 am (L-208) Protein Diffusion in Charged Polyacrylamide Gels:

Visualization and Modeling—R.K. Lewus, Giorgio Carta,

University of Virginia, Charlottesville, VA, USA

12:10-3:30 pm BREAK / EXHIBITS / POSTERS

Location: Gold Rush Ballroom and Pacific Suite on lower level of hotel

12:30-1:45 pm WORKSHOP on Gradiflow—A Revolution in Protein Purification

Sponsored by Gradipore—complimentary lunch provided


Pre-registration at Gradipore’s booth is required

12:30 – 1:45 pm WORKSHOP on Recent Advances in Recycling Preparative HPLC

Sponsored by DyChrom — complimentary lunch provided



2:00-3:15 pm POSTER SESSION

Location: Pacific Suite (lower level of hotel)

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TUESDAY, MAY 25


CHAIR: DR. JANA JACOBSON

3:30 pm (L-209) The Cost of Not Optimizing the Design and Operating

Parameters in Process Chromatography—Anita M. Katti,

Engineering and Process Chromatography Solutions, Boulder,

CO, USA

3:50 pm (L-210) Titanium Dioxide - Unique Selectivity for Preparative Liquid

Chromatography—B. Hirthe, Jochen Winkler, G. Benkner,

R. Wittenberg, S. Marmé, Sachtleben, Duisburg, and Ulrich

Trüdinger Consulting, Wiesbaden, GERMANY

4:10 pm (L-211) Preparation and Characterization of a New High Capture, High

Capacity Process Media—Jia Li Liao, H. Chen, L. Cummings, S.

Franklin, R. Frost, W.-K. Lam, L. Olech, C. Ordunez, K. Talmadge,

T. Tisch, Bio-Rad Laboratories, Hercules, CA, USA

4:30 pm (L-212) Hydrophobic Displacement Chromatography (HDC):

Applications for Bioprocessing—Kkhurram M. Sunasara,

A.A. Shukla, S.M. Cramer, Rensselaer Polytechnic Institute,

Troy, NY, USA

4:50 pm PAUSE

5:10 pm GENERAL DISCUSSION

6:00-7:00 pm RECEPTION sponsored by NOVASEP


7:00 pm EXHIBIT CLOSES

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WEDNESDAY, MAY 26


7:15-8:30 am WORKSHOP on An Introduction to EasyPack and Geltech Industrial

Chromatography Columns – easy to use, sanitary design columns for

pilot and production scale biotherapeutic manufacturing

Sponsored by PE Biosystems—complimentary continental

breakfast provided


Pre-registration at PE Biosystems’ booth is required

7:15-8:30 am WORKSHOP on Optimization in Industrial HPLC

Sponsored by EKA Chemicals—complimentary continental

breakfast provided

Location: Crystal Room (lobby level of hotel)

Pre-registration at BTR-Separations’ booth is required

CHAIR: DR. FIROZ ANTIA

9:00 am (L-301) An Overall Mass Transfer Design Method for SMB

Chromatography for Amino Acids Separation—Y. Xie, D.J. Wu,

Z. Ma*, N.-H.L. Wang, Purdue University, W. Lafayette, IN, USA;

*Bioanalytical Systems, W. Lafayette, IN, USA

(presented by Ben Hritzko)

9:20 am (L-302-new) SF-SMB (Supercritical Fluid Simulated Moving Bed) Application to

Chiral Separations—Olivier Ludemann-Hombourger, F. Denet,

M. Bailly, R.M. Nicoud, NovaSep SA, 15 Rue du Bois de la

Champelle, BP 50, 54502 Vandoeuvre , FRANCE

9:40 am (L-303) Separation of Phytol-Stereoisomers in a SFC-SMB Plant—

A. Depta, T. Giese, Monika Johannsen, G. Brunner, Technische

Universität Hamburg-Harburg, Hamburg, GERMANY

10:00 am (L-304) Enantiomer Separation by SMB Chromatography: The Case of

Non-Instantaneous Equilibrium at the Solid-Fluid Interface—

Diana C.S. Azevedo, M. Li and A.E. Rodrigues, LSRE, University

of Porto, Porto Codex, PORTUGAL

10:20 am BREAK

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WEDNESDAY, MAY 26


CHAIR: DR. JOAN NEWBURGER

10:50 am (L-305) A Novel SMB-Method for Further Reduced Solvent

Consumption—Thomas B. Jensen, T. Reijns, H.A.H. Billiet,

L.A.M. van der Wielen, Delft University of Technology, THE

NETHERLANDS

11:10 am (L-306) Preparative Continuous Annular Chromatography (P-CAC) as

a Continuous Downstream Process for Biotechnology—

Juergen Wolfgang, A. Prior, Prior Technologie Ges.m.b.H, Götzis,

AUSTRIA

11:30 am (L-307) Applying Preparative Centrifugal Counter-Current

Chromatography as Purification Tool for Fermentation

Products—W.W. Fan, Ernst Küsters, C.P. Mak, NOVARTIS

Pharma AG, Basel, SWITZERLAND

11:50 pm (L-308) Preparative Continuous Annular Biochromatography—

R. Giovannini, Ruth Freitag, ETH Lausanne, Ecublens,

SWITZERLAND

12:10 pm (L-309) A Nine Zone SMB for the Recovery of Glucose and Xylose From

Biomass Hydrolyzate—Z. Ma, N.-H.L. Wang, R. Wooley*,

*National Renewable Energy Laboratory; Purdue University, W.

Lafayette, IN, USA

12:30 pm BREAK

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WEDNESDAY, MAY 26


CHAIR: PROF. GYULA VIGH

2:00 pm (L-310) Assessing the Use of Process Liquid Chromatography in

Production of Drug Substances—Gregor Mann, Schering, Berlin,

GERMANY

2:20 pm (L-311) Monolithic Macroporous Poly(Styrene-CO-Divinylbenzene)

Columns for Rapid or High Throughput Reversed Phase

Separation of Proteins—Shaofeng Xie, R. Allington, B. Allington,

J. Algaier, V. Pakhye, A.G. Craske, F. Svec*, J.M.J. Fréchet*,

ISCO, Lincoln, NE, USA; *University of California, Berkeley, CA,

USA

2:40 pm (L-312) Development, Optimization, and Scale-Up of a

Chromatographic Purification for a Synthetic Oligonucleotide—

Robert A. Picciotti, Jr., J.R. Fisher, J.K. O’Donnell, R.E. Rosen,

J.J. Maikner*, M.K. Kraus**, S.J. Iuliano, A.E. Pressley, TosoHaas,

Montgomeryville, PA, USA; *Rohm and Haas Company, Spring

House, PA, USA; **TosoHaas GmbH, Stuttgart, GERMANY

3:00 pm (L-313) Influence of Column Design on Process-Scale Ion-Exchange

Chromatography—Peter R. Levison, A.K. Hopkins, P. Hathi,

Whatman International Ltd., Kent, UK

3:20 pm (L-314) Pharmaceutical Purification Facility Design and Considerations

—Lou Bellafiore, K. Henretta, TechniKrom, Evanston, IL, USA

3:40 pm MEETING ADJOURNS

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— POSTER PRESENTATIONS — MONDAY, MAY 24

Location: Pacific Suite Rooms / Session Times: 2:00 – 3:15 p.m.

P-113 Aqueous Coefficient Calculations for Alkanes, Acetaminophenes and

Barbiturates—F. Torrens, J. Sánchez-Marín, I. Nebot-Gil, Universitat de València,

Burjassot, SPAIN

P-114 Preparative Chromatographic Resolution of Enantiomers Using Polar Organic

Solvents with Polysaccharide Chiral Stationary Phases—L. Miller, R. Fronek,

C. Orihuela, Searle, Skokie, IL, USA

P-115 Predictable, Reproducible Scaling of Microbore, Analytical, and Preparative

Liquid Chromatography—D. Lee, S. Liao, Hamilton Company, Reno, NV, USA

P-116 Packing Procedures and Properties of VYDAC Media in the Axial Compression

Column—M.K. Li, P. Kostel, Vydac, The Separation Group, Hesperia, CA, USA

(withdrawn)

P-117 SF-SMB (Supercritical Fluid Simulated Moving Bed) Application to Chiral

Separations—F. Denet, W. Hauck, R.M. Nicoud, NovaSep SA, Vandoeuvre

l’Nancy, FRANCE

P-118 How To Choose A Simulation Model to Represent the SMB Process?—

O. Ludemann-Hombourger, R.M. Nicoud, M. Bailly*, * LSGC CNRS-ENSIC,

Nancy, Cedex, FRANCE; NovaSep SA, Vandoeuvre l’Nancy, FRANCE

P-119 Modeling the Steady State Recycling Process Comparison of Theory and

Experiment—I. Quinones-Garcia, G.Guiochon†, C.M. Grill*, L. Miller**,

University of Tennessee, Knoxville, TN, USA and †Oak Ridge National

Laboratory, Oak Ridge, TN, USA; *R&S Technology, Wakefield, RI, USA;

**Searle, Skokie, IL, USA

P-120 Development of a Preparative Chiral Separation for Racemic Thalidomide in

Normal Phase—D.-R. Wu, K. Lohse, BTR Separations, Wilmington, DE, USA

P-121 High-Concentration Band Profiles and System Peaks in a Ternary Solute

System—I. Quiñones, J.C. Ford*, G. Guiochon, University of Tennessee,

Knoxville, TN, USA and Oak Ridge National Laboratory, Oak Ridge, TN, USA;

*Indiana University of Pennsylvania, Indiana, PA, USA

P-122 Analytical and Preparative-Scale Isoelectric Focusing Separation of

Enantiomers—P.V. Glukhovskiy, G. Vigh, Texas A&M University, College

Station, TX, USA

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P-123 Effect of Column to Column Differences on the Performance of Simulated

Moving Bed Separators—J. Frickea, K. Mihlbachlera,b, T. Yuna,b, J. Strubec,

H. Schmidt-Traubc, G. Guiochona,b; aUniversity of Tennessee, Knoxville, TN,

USA; bOak Ridge National Laboratory, Oak Ridge, TN, USA; cUniversity of

Dortmund, GERMANY

P-124 Enantiomer GC Separation of -Ionone Using Cyclodextrin Derivatives—

F. Quattrini, G. Biressi, M. Juza, M. Mazzotti, C. Fugantiš, M. Morbidelli, ETH

Zentrum, Zürich, SWITZERLAND; Politecnico di Milano, Milano, ITALY

P-125 Characterization of Ionic Binding on Base Degraded Size Exclusion Chromato-

graphy Resin—S. Ramachandran, E. Ford, Genentech, S. San Francisco, CA, USA

P-126 Preparative Chiral Purification of Enantiomers Using Covalently-Bonded Chiral

Stationary Phases—D.-R. Wu, K. Lohse, L. Lin*, BTR Separations, Wilmington,

DE, USA; MediChem Research, Lemont, IL, USA

P-127 Separation of 1-Phenyl-1-Propanol Enantiomers Using Simulated Moving Bed—

S. Khattabi*, D.E. Cherrak*†, J. Fischer#, K. Mihlbachler*†, G. Guiochon*†; *University of Tennessee, Knoxville, TN, USA; †Oak Ridge National Laboratory,

Oak Ridge, TN, USA; #University of Pardubice, Pardubice, CZECH REPUBLIC

P-128 Characterization and Scale-up of a New Weak Cation Exchanger CM Sepharose™

High Performance—B. Forsberg, J. Gustavsson, I. Langerlund, E. Larsson,

L. Lundh, Amersham Pharmacia Biotech, Uppsala, SWEDEN

P-129 Design of a Simulated Moving Bed for Sucrose-Betaine Separation—

S. Giacobello, G. Storti, G. Tola, University of Cagliari, Cagliari, ITALY

P-130 Calibration Considerations for Determination of On Column Concentration

Distributions Using Photographic Detection—S. Broyles, A. Shalliker,

G. Guiochon, University of Tennessee, Knoxville, TN, USA and Oak Ridge

National Laboratory, Oak Ridge, TN, USA

P-131 Strategies for High Throughput HPLC Purification for Combinatorial Chemistry

—J. Carmody, U. Neue, R. Crowley, C. Andrews, Waters, Milford, MA, USA

P-132 Titanium Dioxide – Properties and Applications in LC—J. Winkler, S. Marmé,

B. Hirthe, G. Benkner, R. Wittenberg, Sachtleben, Duisburg, GERMANY;

U. Trüdinger, Consulting, Wiesbaden, GERMANY

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P-133 Evaluation of Commercial Simulated Moving Bed Units Through A Binary Test

Separation—M. Juza, CarboGen Laboratories, Aarau, SWITZERLAND

P-134 Kromaguide: A Tool for Simulation and Optimization of Industrial HPLC—

L. Torstensson, P. Jageland, EKA Chemicals, Bohus, SWEDEN

P-135 Determination of the Thermodynamic Contribution to Peak Asymmetry of Basic

Solutes in Reversed-Phase Liquid Chromatography—B.J. Stanley, California State

University, San Bernardino, CA, USA

P-136 Advances in Large Scale Chromatography Packing—G. Purdom, N. Dickson,

P. O’Neil, F. Mann, Millipore, Gloucester, ENGLAND

P-137 Comparison Between Experimental and Calculated Band Profiles of 3-Chloro-1-

Phenyl-1-Propanol Enantiomers on Chiral Stationary Phase. Influence of Mass

Transfer Kinetic—D.J. Cherrak*, S. Khattabi*, G. Guiochon*+, *University of

Tennessee, Knoxville, TN, USA; +Oak Ridge National Lab., Oak Ridge, TN, USA

P-138 Generating Trace Impurity Samples From Omeprazole by Semi-Preparative

PCSFC—I. Bhoir, S. Patil, S. Dhuri, M. Sundaresan, C.B. Patel Research Centre

for Chemistry and Biological Sciences, Mumbai, INDIA (withdrawn)

P-139 Preparation by PCSFC of Zopiclone Oxides From Zopiclone on a Semi-Preparative

Scale by Modifier Programming—V. Bari, I.C. Bhoir, V. Mudliyar, M.

Sundaresan, C.B. Patel Research Centre for Chemistry and Biological Sciences,

Mumbai, INDIA (withdrawn)

P-140 Design of a Carousel Process for Removing Cesium From SRS Wastes Using

Crystalline Silicotitanate Ion Exchanger—B.J. Hritzko, N.-H.L. Wang, D.D.

Walker*, Purdue University, W. Lafayette, IN, USA; *Westinghouse Savannah

River Co., Aiken, SC, USA

P-141 Reduced Costs and Increased Revenues of Biopharmaceutical Purification

Process—S. Saxena, C. Boomla (Sepragen Corporation, Asia), Sepragen

Corporation, 30689 Huntwood Ave., Hayward, CA, USA

P-142 Preparative Electrochromatography—J.-R. Chen, R.N. Zare, Stanford University,

Stanford, CA, USA

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— POSTER PRESENTATIONS — TUESDAY, MAY 25


P-213 Charcoal De-Colorization Using Pre-Packed Disposable Cartridges—

S. Ghassemi, P.C. Rahn, Biotage, Charlottesville, VA, USA

P-214 Purification of Peptides by Flash Technique—S. Ghassemi, P.C. Rahn, Biotage,

Charlottesville, VA, USA

P-215 Purification and Characterization of the Vascular Endothelial Growth Factor

Receptor Kinase Domain—W.D. Holmes, W.-J. Chen, R. Davis, A. Edison,

C. Hoffman, E. Horne, S. Kadwell, B. Ellis, Glaxo Wellcome, Research Triangle

Park, NC, USA

P-216 Phytic Acid as an Efficient Low-Molecular-Weight Displacer for Anion

Exchange Displacement Chromatography of Proteins—Q. Luo, J.D. Andrade,

University of Utah, Salt Lake City, UT, USA

P-217 Polycations as Displacer in High-Performance Bioseparation—B. Schmid,

C. Wandrey, R. Freitag, ETH Lausanne, Ecublens, SWITZERLAND

P-218 Hydroxyapatite Chromatography - A Solution for Large Scale Plasmid DNA

Purification?—J. Wright, J. van Adrichem, F. Wurm, R. Freitag, ETH Lausanne,

Ecublens, SWITZERLAND

P-219 Increasing Efficiency in Purification Using Automated LC/SPE Coupling

Techniques and Parallel Chromatography—R. God, C. Heuer, AnalytiCon AG,

Potsdam, GERMANY

P-220 Specific Capture of Antibodies Using Hydrophobic Charge Induction

Chromatography—M. Taylor, L. Guerrier, E. Boschetti, BioSepra, Marlborough,

MA, USA

P-221 Chromatographic Assay of Pharmaceutical Compounds Under Column-

Overloading With Dual Wavelength Detection—J. Zhu, C. Coscolluella, J. Rosen,

Schering-Plough Corp., Kenilworth, NJ, USA

P-222 Studies on Chromatographic Fractionation of Trichoderma Reesei Cellulases by

Hydrophobic Interaction—C.T. Tomaz, J.A. Queirox, Universidade da Beira

Interior, Covilhã, PORTUGAL

19



P-223 Adsorption of Proteins on Porous, High-Capacity Acrylamido-Based Ion-

Exchange Media—A.K. Hunter, G. Carta, University of Virginia, Charlottesville,

VA, USA

P-224 Preparative Electrophoresis: Strategies for Optimization of Protein Purification—

D.B. Rylatt, M.D. Napoli, D. Ogle, A. Gilbert, L. Lim*, C.H. Nair, Gradipore Ltd.,

Sydney, AUSTRALIA; *Macquarie University Center for Analytical

Biotechnology, Sydney, AUSTRALIA

P-225 Synthesis of Recombinant Human -Amyloid Peptides 1-42 and 1-40 in E. coli—

E.B. Neuhaus, N.K. Menon, R.A. Makula, A.E. Przybyla, University of Georgia,

Athens, GA, USA

P-226 Anion Exchange Chromatography to Remove Contaminants from Albumin

Preparations—S. Trukawinski, R. Sharma, H.-I. Paul, Bayer Corporation, Clayton,

NC, USA

P-227 A Survey of Selectivity Differences Between Fractogel® EMD Anion Exchange

Chromatography Resins for Plasmid DNA—T.L. Chao, B.R. Cabrera, L. Britsch*,

EM Industries, Gibbstown, NJ, USA; *Merck, Darmstadt, GERMANY

P-228 Chromatography Simulation as a Process Development Tool—J.S. Bowers,

P.M. Gioioso, D.C. Wylie, M. Voloch, Schering-Plough Research Institute,

Union, NJ, USA

P-229 Process Economics Aspects of RPC Peptide Purifications—K.C. Deissler,

M.K. Kinzey, R.E. Rosen, J.F. Fisher*, M. Kraus*, J.J. Maikner, Rohm and Haas

Company, Springhouse, PA, USA; *TosoHaas, Montgomeryville, PA, USA

P-230 On-Line Substrate Analysis with Selective Biocart Enzyme Cartridges—

J.D. MacFarlane, W. Schaefer*, H.-R. Howind*, JM Science, Grand Island, NY,

USA; *Anasyscon Instrumentelle Analysentechnik GmbH, Hannover, GERMANY

P-231 Very Fast Separation of Synthetic Oligonucleotides by Ion-Exchange

Chromatography on CIM Ultra-Short Columns—A. Strancar1, M. Prhavc2,

S. Jaksa2, M. Barut1, J. Jancar1, A. Podgornik3, J. MacFarlane4, 1BIA Separations

d.o.o., Ljubljana, SLOVENIA; 2National Institute of Chemistry, Ljubljana,

SLOVENIA; 3BIA d.o.o., Ljubljana, SLOVENIA; 4JM Science, Grand Island, NY,

USA

20



P-232 Ultra-Fast Purification of Human Plasma Proteins Using Ultra-Short CIM

Columns—M. Barut1, A. Strancar1, A. Podgornik2, A. Buchacher3, D. Josic3,

J.D. MacFarlane4, 1BIA Separations d.o.o., Ljubljana, SLOVENIA; 2BIA d.o.o.,

Ljubljana, SLOVENIA; 3Octapharma ProdmbH, Wien, AUSTRIA; 4JM Science,

Grand Island, NY, USA

P-233 Ultra-Fast HPLC Prep-Scale Purification of Endoxylanases Using CIM DEAE

Columns—R.M. Logar1, M. Barut2, F.V. Nekrep1, A. Strancar2, J.D. MacFarlane3, 1University of Ljubljana, Domzale, SLOVENIA; 2BIA Separations, d.o.o.,

Ljubljana, SLOVENIA; 3JM Science, Grand Island, NY, USA

P-234 The Study of Atrazine Detection by ELISAs—G. Coman, M. Pop, C. Draghici,

Transilvania University of Brasov, Brasov, ROMANIA

P-235 TLC Resolution of DL Amino Acids and Their Derivatives on Chrial Stationary

Phases—C. Draghici, G. Coman, G. Roman, M. Pop, Transilvania University of

Brasov, Brasov, ROMANIA

P-236 Environmental Analysis by Ion Chromatography—M.L. Gupta, V.B. Apale,

P.K. Shrivastave, N. Upadhyay, M. P. Pollution Control Board, Bhopal, INDIA

P-237 The Effect of NaOH Sanitization of Gel Capacity, Column Lifetime and

Inactivation of Viruses and Microorganisms—E. Shane, D. Vu, C. Oliver,

MedImmune, Gaithersburg, MD, USA

P-238 A New Hydrophobic Interaction Chromatography Resin for the Purification of

Biomolecules—R.E. Rosen, J.K. O’Donnell, J.R. Fisher, M.K. Kraus*, S.J. Iuliano,

R.A. Picciotti, A.E. Pressley, TosoHaas, Montgomeryville, PA, USA; *TosoHaas

GmbH, Stuttgart, GERMANY

P-239 Fast Flow and Linear Scalability of Chromatography in Production at Low Costs—

S. Saxena, Marcel Raedts (Sepragen Corporation, Europe), Sepragen Corporation,

30689 Huntwood Ave., Haywood, CA, USA

P-240 Fast Direct Capture From Crude Feed Streams—S. Saxena, Sepragen Corporation,

30689 Huntwood Ave., Hayward, CA, USA

21

E X H I B I T O R S

AMERSHAM PHARMACIA BIOTECH

800 Centennial Ave., P.O. Box 1327, Piscataway, NJ 08855-1327

phone: 800-526-3593 / fax: 800-FAX-3593

Exhibiting: Amersham Pharmacia Biotech is a supplier of equipment and chemicals to the

biopharmaceutical industry. The Separations unit provides chromatography resin and equipment

for the production of biopharmaceuticals.

ANALYTICON AG

Box 5926, Kingwood, TX 77325

phone: 281-361-7393 / fax: 281-361-6885

Exhibiting: SEPBOX chromatography systems for purification of drug substances and

fractionation of natural products. Automated on-line coupling of preparative HPLC and Solid

Phase Extraction that leads to easy access of pure compounds in a buffer free organic

environment. Multi-Parallel HPLC for automated high throughput purification.

BIOSEPRA

111 Locke Drive, Marlborough, MA 01752

phone: 800-752-5277 / fax: 508-357-7595

Exhibiting: Biochromatography Media.

BIO-RAD LABORATORIES

2000 Alfred Nobel Drive, Hercules, CA 94547

phone: 510-741-1000 / fax: 510-234-2642

Exhibiting: Discover Bio-Rad. We are presenting our full line of process chromatography

supports, including the Macro-Prep ion exchangers and ceramic hydroxyapatite. Learn more about

our new size exclusion supports and our UNO continuous bed columns. Inquire about our custom

manufacturing services, including specialty buffers and custom chromatography supports.

BTR-SEPARATIONS

3521 Silverside Road, Concord Plaza-Quillen Bldg., Wilmington, DE 19810

phone: 800-766-6141 / fax: 302-695-5026

Exhibiting: BTR-Separations supplies innovative solutions to purification problems. The company

is a preferred source of products and technical support for chromatographers in the pharmaceutical

and biotechnology industries. BTR-Separations will feature new Chiral Media and Chiral

Development Services, in addition to our other process development and process media products -

ZORBAX®Classic, ZORBAX LP, Kromasil® and IMPAQ®. These products are considered

benchmarks for excellence in performance worldwide and are available in columns for process

development, prep-columns and bulk media.

22

E X H I B I T O R S

CHIRAL TECHNOLOGIES, INC.

730 Springdale Drive, P.O. Box 564, Exton, PA 19341

phone: 800-624-4725 or 610-594-2100 / fax: 610-594-2325

Exhibiting: Chiral Technologies, Inc. specializes in enantiomeric separation products and services

for the pharmaceutical and related industries. Visit our booth to discuss recent advances in bulk

chiral stationary phases that lead to increased productivity and improved economics for SMB

processes. Separation service arrangements available for gram to metric ton quantities.

DYCHROM

473 Sapena Court, Suite 8, Santa Clara, CA 95054

phone: 408-988-7611 / fax: 408-988-4536

Exhibiting: DyChrom is a leading supplier of specialty liquid chromatography products. We

introduced this year Model LC-918 Recycling Preparative HPLC; a cost effective system for

purification of complex samples. DyChrom is also a dealer for two types of countercurrent

chromatographs; droplet countercurrent and rotation locular countercurrent.

GRADIPORE

Riverside Corporate Park, 35-105 Delhi Road, North Ryde, Sydney NSW 2113, AUSTRALIA

phone: 011-61-2-98782777 / fax: 011-61-2-98050624

Exhibiting: Gradipore Ltd. will be exhibiting their Gradiflow protein purification technology.

Gradiflow is a novel scaleable technology for separation of proteins and other charged

macromolecules. This system is the first to harness the resolution of polyacrylamide

electrophoresis at a preparative scale. The unique tangential flow membrane configuration allows

the separations of macromolecules by either size and or charge. The system also has the capacity

to concentrate and desalt.

HAMILTON COMPANY

4970 Energy Way, Reno, NV 89502

phone: 775-858-3000 / fax: 775-856-7259

Exhibiting: Hamilton manufactures 16 polymeric HPLC packings for a wide variety of

applications. Column diameters from 1.0 to 101.6 mm. Bulk material is available in particle sizes

of 10, 12-20, 20-30, 30-50 and 50-75 ums. Custom synthesis and modification services are

available as we manufacture all our HPLC supports.

HITACHI INSTRUMENTS, INC.

3100 North 1st Street, San Jose, CA 95134

phone: 408-432-0520 / fax: 408-432-8258

Exhibiting: NovaPrep semi-preparative HPLC systems. Nova Prep 200 features a flow rate

range of 1-200 ml/min while Nova Prep 800 ranges from 8-800 ml/min; both systems are totally

inert. Isocratic, step gradient, low pressure gradient and high pressure gradient configurations are

available. A wide range of columns and sorbents are available.

23

E X H I B I T O R S

JM SCIENCE, INC.

P.O. Box 250, 355 Lang Blvd., Grand Island, NY 14072

phone: 716-774-8706 / fax: 716-774-8708

Exhibiting: New Convective Interaction Media (CIM) semi-prep and preparative tubes for very

fast separations of proteins, oligonucleotides and other biomolecules. Also new HPLC pump for

pilot and production scale separations. Shodex preparative columns up to 30cm diameter for all

scales of separation. New automated bioprocessing system for monitoring key metabolites for

days or weeks.

KNAUER (Dr. Ing. Herbert Knauer GmbH)

Hegauer Weg 38, 14163 Berlin, GERMANY

phone: 011 49 30 80 97 27 50 / fax: 011 49 30 8 01 50 10

Exhibiting: KNAUER introduces the high performance preparative HPLC pump K-1800. It is the

smallest HPLC pump in its performance class with flow rates up to 1,000mL/min. KNAUER

offers a wide range of photometric detectors and easy to handle software for individual needs.

MALLINCKRODT BAKER, INC.

222 Red School Lane, Phillipsburg, NJ 08865

phone: 908-859-2151 / fax: 908-859-9318

Exhibiting: BAKERBOND Chromatography Columns and Packings will be featured with

products for biochromatography and synthetic peptide purification, and our new line of spherical

products for small molecule separations. Detailed information on the CYCLE-TAINER Dispense

system and a complete listing of high purity solvents and reagents for scale-up will also be

presented.

MODcol CORPORATION

845 Stewart Drive, Sunnyvale, CA 94068-4514

phone: 408-530-0010 / fax: 408-530-0011

Exhibiting: MODcol has recently developed "Axial Compression Spring Loaded" (Patents

Pending) preparative HPLC columns. Designed for on-site, efficient self-slurry packing with the

aid of the pneumatic "MultiPacker" (Patents Pending)--products are unique since several

preparative HPLC columns may be packed sequentially. Also offering Patented Fixed bed

modular stainless steel columns for preparative HPLC, Pilot Plat and Industrial large-scale LC at

various lengths, I.D. and ASME Coded by request. MODcol is renown worldwide for slurry

packing of various microparticulates in its modular columns using proprietary procedures, and

guarantees efficient slurry packing, as a paid service, with excellent resolution and low pressure

drop. Various MODadapters are available to extend the length of the modular columns.

24

E X H I B I T O R S

NOVASEP, INC.

480 S. Democrat Road, Gibbstown, NJ 08027

phone: 609-423-6718 / fax: 609-423-1720

Exhibiting: Conception of turn-key solutions for industrial chromatographic separations: • SMB or

HPLC systems (with liquid, gas or supercritical eluents, low and high pressure operations), with

fully integrated solvent recycling and storage units. • Complete services for all applications

requiring large-scale purification (pharmaceuticals, food additives, fine chemicals), from

feasibility study to process development, optimization and validation, and custom purification

under GMP.

PDR-CHIRAL

3 Old Meadow Way, Palm Beach Gardens, FL 33418

phone: 561-625-2645 / fax: 561-625-2646

Exhibiting: PDR-Chiral Inc presents its new Advanced Laser Polarimeter for the measurement of

optically active compounds. The diode laser-based detector provides information about

enantiomeric ratio, in real-time, even without complete resolution of the enantiomers. The low

noise, opto-electronic design results in a detection limit of 25-microdegrees with the capability of

determining better than 1% enantiomeric excess for most enantiomers. The instrument can be used

in conjunction with LC, LC-MS, FIA and SFC. It can also be used as a monitor for preparative-

scale chromatography and process control.

PE BIOSYSTEMS

500 Old Connecticut Path, Framingham, MA 01701

phone: 508-383-7482 / fax: 508-383-7885

Exhibiting: PE Biosystems introduces a new line of process scale chromatography columns. These

columns, manufactured under a 5 year agreement with Verdot Industries of Riom, France, are

designed for ease of use and reliability, and are accompanied by full regulatory support

documentation. Available in glass, stainless steel, or acrylic, these columns all feature a center

screw mechanism and inflatable seal which allows for the simplest and most efficiently packed

columns. A consistent and sanitary design is used in all columns from 90mmID to 2000mmID for

the best scale-up results. Please come by our booth, and attend our workshop where we will

introduce the unique features of these products.

PHENOMENEX

2320 West 205th Street, Torrance, CA 90501

phone: 310-212-0555 / fax: 310-328-7768

Exhibiting: Offering a complete line of HPLC colulmns and bulk materials for preparative and

process chromatography utilizing revolutionary new silica and bonding technology. LUNA 100A

and Jupiter 300A (proteins/peptides) materials provide ultra-high performance characteristics such

as: 1.5-10.0 pH stability, exceptional peak shape, increased loadability, and superior mechanical

strength for maximum efficiencies. Available world-wide in economical 10 and 15 particles in

packed columns, or 1-1000 Kg bulk quantities.

25

E X H I B I T O R S

SEPRAGEN CORPORATION

30689 Huntwood Avenue, Hayward, CA 94544

phone: 510-476-0650 / fax: 510-476-0655

Exhibiting: Sepragen Corp. is a leading provider of Automated Scale-Up Instruments, Columns

and Media to the biopharmaceutical Industry, “from lab to manufacturing”. Sepragen also

develops and sells processes and products using its proprietary technology for the dairy, juice and

nutraceutical markets.

TECHNIKROM, INC.

1801 Maple Avenue, Evanston, IL 60201

phone: 800-865-4100 / fax: 800-293-5059

Exhibiting: TechniKrom provides cGMP industrial bioprocessing equipment for LPLC, HPLC,

automated buffer preparation, UF, MF, expanded bed/capture, SMB, fermentation and peptide

synthesis. Walk away automation capability is permitted through the innovative use of precise

feedback and control available on all equipment. Our staff of engineers and chemists provide

services including equipment customization, facility layouts, process method development, scale-

up and contract GLP/GMP purifications.

TOSOHAAS

156 Keystone Drive, Montgomeryville, PA 18936

phone: 215-283-5000 / fax: 215-283-5035

Exhibiting: TosoHaas features a wide range of liquid chromatography products for the analysis

and purification of proteins, peptides, enzymes, oligonucleotides, antibodies and small molecules.

Our line of Amberchrom, Toyopearl and TSK-GEL pre-packed columns and bulk media are

designed to provide seamless scale-up from laboratory to process scale.

VARIAN ASSOCIATES

3045 Hanover Street H-111, Palo Alto, CA 94304

phone: 650-424-5848 / fax: 650-858-0480

Exhibiting: A full line of Preparative HPLC products will be displayed: The state-of-the-art SD-1

solvent delivery system can repetitively separate mg to gram quantities of material in automated,

unattended fashion, increasing lab throughput and reducing costs. The RamPak Axial

Compression Packing Station can be used to pack 41 or 77 mm ID preparative columns.

VYDAC/THE SEPARATIONS GROUP

17434 Mojave Street, Hesperia, CA 92345

phone: 800-247-0924 / fax: 888-244-6107

Exhibiting: VYDAC offers preparative HPLC columns and bulk reversed phase silicas providing

simple, high performance solutions to purification problems. These materials, part of the family of

materials used in the research laboratory, offer simple scale-up of laboratory procedures and give

production laboratories the same level of performance, purity and recovery available to the

research scientist. Packed columns are available in I.D.s up to 100 mm; bulk materials are

available for process scale separations in 100-kilogram lots.

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E X H I B I T O R S

WATERS CORPORATION

34 Maple Street, Milford, MA 01757

phone: 508-478-2000 / fax: 508-482-2674

Exhibiting: Waters continues to advance HPLC instrumentation, software and chemistries. Waters

Delta 600 PrepLC System provides powerful, yet flexible analytical to semi-prep capabilities.

Waters DeltaPrep 4000 System extends the analytical to prep capability into true preparative scale

with programmable flow rates. Waters PrepLC 2000 is a dedicated high throughput preparative

LC system. Waters has a full line of Preparative Chromatography Columns.

WHATMAN, INC.

401 West Morgan Road, Ann Arbor, MI 48108

phone: 800-343-5853 / fax: 734-665-3516

Exhibiting: The Whatman chromatography portfolio includes the Express-Ion range of high flow

rate media, including the Express-Ion Mini-Columns. This media is versatile, easy to use, and cost

effective in industrial scale purification processes. Express-Ion products are ideal where

processing times of large volume feedstocks are to be minimized.

YMC CORPORATION

Exhibiting: For full selection of YMC products, see Waters Corporation booths.

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