111/18/2015 Bacterial Genetics Filename BactGene.ppt.
-
Upload
peregrine-lawson -
Category
Documents
-
view
227 -
download
0
Transcript of 111/18/2015 Bacterial Genetics Filename BactGene.ppt.
1
04/20/23
Bacterial GeneticsBacterial Genetics
Filename BactGene.ppt
2
04/20/23
Background ConceptsBackground Concepts
Structure & Function of DNA & RNA
Genetic code Transcription & translation Protein synthesis Gene Regulation: operators,
promotors , repressors
Filename: DNA.ppt
3
04/20/23
Lecture OutlineLecture Outline
Genetic Flow Genetic change
Transformation Conjugation Transduction
Plasmids Transposons
4
04/20/23
Genetic FlowGenetic Flow
Between Generations of Cells Within Cells
5
04/20/23
Genetic Flow Between Genetic Flow Between GenerationsGenerations
6
04/20/23
Genetic flow within a Genetic flow within a GenerationGeneration
7
04/20/23
The Genetic CodeThe Genetic Code
Genetic information is stored in DNA sequences found in
Chromosomes copy/cell DS- DNA Circular
Plasmids
8
04/20/23
PlasmidPlasmid
Smaller than chromosomal DNA
Contains only a few genes
independent of chromosome
circular multiple copies!!
9
04/20/23
Griffith’s Transformation Griffith’s Transformation Exp.Exp.
Background Pathogenic bacteria have capsule Nonpathogenic bacteria have no capsule
Experiment: inject live pathogens live nonpathogens dead pathogens live pathogens+dead nonpathogens
10
04/20/23
Bacterial TransformationBacterial Transformation
11
04/20/23
Live PathogensLive Pathogens
Mouse Dies
recover pathogens
12
04/20/23
Live non PathogensLive non Pathogens
Mouse lives
Non pathogens recovered
13
04/20/23
Dead PathogensDead Pathogens
Mouse lives
No Pathogens
14
04/20/23
Live NonPathogens+Live NonPathogens+Dead PathogensDead Pathogens
Live Pathogens!!
15
04/20/23
Genetic Basis of Genetic Basis of transformationtransformation
16
04/20/23
Recipient cell takes up Recipient cell takes up DNADNA
17
04/20/23
Recombination OccursRecombination Occurs
18
04/20/23
Bacterial ConjugationBacterial Conjugation
Sex and the single cell
19
04/20/23
Bacterial ConjugationBacterial Conjugationin E. coliin E. coli
20
04/20/23
Conjugation between F- Conjugation between F- & F+& F+
21
04/20/23
Replication & Transfer Replication & Transfer of F Factorof F Factor
22
04/20/23
F- cell converted to F+F- cell converted to F+
23
04/20/23
F factor & ChromosomeF factor & Chromosome
Recombination
24
04/20/23
F factor produces an HfrF factor produces an Hfr
25
04/20/23
Conjugation Between Hfr Conjugation Between Hfr & F-& F-
26
04/20/23
Replication & TransferReplication & Transfer
27
04/20/23
Recombination in F- CellRecombination in F- Cell
28
04/20/23
Viral TransductionViral Transduction
29
04/20/23
Phage Infects Donor CellPhage Infects Donor Cell
30
04/20/23
Bacterial Chromosome Bacterial Chromosome Broken DownBroken Down
31
04/20/23
Bacterial DNA Trapped Bacterial DNA Trapped in Phage Coatin Phage Coat
32
04/20/23
Bacterial DNA Infects Bacterial DNA Infects New CellNew Cell
33
04/20/23
Recombination can Recombination can OccurOccur
34
04/20/23
TransposonsTransposons
“Jumping Genes” First discovered in corn Genetic element Moves other genes from place to
place on the chromosome Moved genes may be regulated
differently
35
04/20/23
Insertion Sequence Insertion Sequence “IS1”“IS1”
36
04/20/23
Complex TransposonComplex Transposon
37
04/20/23
R Plasmid “R1”R Plasmid “R1”