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Varian 920-LC Integrated Analytical HPLC

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Varian 920-LC What Is It?

• Compact, stylish automated analytical HPLC

– Pre-plumbed and factory tested for the highest confidence

– Single cable communications

– Each system comes with solvent delivery system, autosampler and either UV Vis or PDA detector

– Options of a second Fl or RI detector and column heater

• Virtually pulse-free dual piston pump

– Does not require a pulse damper

• State-of-the-art low pressure quaternary gradient module with built-in degasser

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Varian 920-LC What Is It?

• Autosampler with cooling option

– Synchronised injection for outstanding retention time reproducibility

– Compatible with 200 x 2 mL HPLC vials or 3 microplates

• Either sensitive dual UV-Vis or PDA detector

– Optional second detector - fluorescence or RI

• High precision (<0.15 °C) dual column heater available

– Low delay solvent pre-heater for improved heat transfer

– Optional column switching and electric injection valve

• NEW improved instrument interface on GalaxieTM software

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Varian 920-LC Silver Bullets

• Fast LC with superb performance optimised for sub 3 m columns

– Improved throughput without compromising robustness

• Pulse-free solvent delivery– No need for a pulse damper

• High sensitivity detection• Full system level computer controlled AIQ (validation)

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0.1 0.2 0.3 0.4 0.60.5 0.7 0.8 0.9 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.8

mAU

RT [min]

100

90

80

70

60

110

40

30

50

20

0

10

Conditions

Mobile Phase: Water Acetonitrile GradientDetection: UV DetectionTemperature: 60°CFlow-Rate: 1,6mL/minColumn: Varian Ultra 3um 50x2,0mm

acet

ophe

none

Pro

piop

heno

ne

vale

roph

enon

e

benz

ophe

none

hexa

noph

enon

e

hept

ophe

none

octa

noph

enon

e

Varian 920-LC Fast LC Phenones

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Varian 920-LC Fast LC PAHs

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Varian 920-LC Fast LC Performance

• Dual piston quaternary pump produces fast gradients – even at 200 L/min

• Virtually pulse-free pumping without pulse damper

• Outstanding gradient compositional accuracy give retention time precision at the <1% level

• Built-in vacuum degas maintains pump prime and enhanced injection reliability

• Accurate flow from 0.1 to 10 mL/min for 2 to 4.6 mm columns

• Overlay of 10 chromatograms

• 10% steps at 1 mL/min

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Varian 920-LC Autosampler

• Needle-in-flow path geometry for low carry-over (<0.01%)

• Typical inj precision (< 0.3% at 10 L)

• Optimized flow paths for fast injection turn-around

• Synchronised injection for best RT reproducibility

• Programmable wash setting for low carry-over

• Injection volumes from 1 uL to 50 L

• Cooled option available

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Varian 920-LC Superb Washout

Blank injection <0.01% carryover

125 µg/mL Caffeine

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920-LC Autosampler How Does it Work?

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Varian 920-LC Injection Synchronization

-20%

0%

20%

40%

60%

80%

100%

120%

0 0.2 0.4 0.6 0.8 1 1.2 1.4 1.6 1.8 2 2.2

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Sensitive UV-Vis Detectors

Typical Agilent VWD S/N ~ 25

Typical Varian 920-LC UV Vis S/N ~ 38

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Varian 920-LC Fluorescence Detector

• Sensitive Fluorescence detector (S/N > 600 Raman H2O peak baseline method)

• Time programmable for increase sensitivity• Automatic validation with built-in Hg lamp• Low 12 L dead volume flowcell for improved

chromatography

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Heated Column Compartment

• Temperature range from ambient + 10 °C up to 65 °C

– Temperature reproducibility +0.15 °C

– Ensures excellent analytical reproducibility

– Uses a low delay volume pre-heater for efficient heat transfer to mobile phase

– Spacious column compartment easily accommodates 2 x 250 mm columns

– Option with electrically actuated column switching valve and injection valve

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Outstanding Stability

20

22

24

26

28

30

32

34

36

38

40

42

44

46

48

50

11:4

6:00

12:2

2:00

12:5

8:00

13:3

4:00

14:1

0:00

14:4

6:00

15:2

2:00

15:5

8:00

16:3

4:00

17:1

0:00

17:4

6:00

18:2

2:00

18:5

8:00

19:3

4:00

20:1

0:00

20:4

6:00

21:2

2:00

21:5

8:00

22:3

4:00

23:1

0:00

23:4

6:00

0:22

:00

0:58

:00

1:34

:00

2:10

:00

2:46

:00

3:22

:00

3:58

:00

4:34

:00

5:10

:00

5:46

:00

6:22

:00

6:58

:00

7:34

:00

8:10

:00

8:46

:00

9:22

:00

Time

Te

mp

era

ture

(d

eg

ree

s C

)

Mobile phase input Temp

Mobile phase temp inside column

Oven Temp

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System Reproducibility of 50 Injections

Reproducibility <0.8% over 50 injections and 12 hours

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Varian 920-LC Software Usability

• User friendly GalaxieTM software

• Simplified, modern, consistent Instrument interface

• Modular 11 pages

• 920-LC 4 pages!!

• One page real-time status and chromatogram

• Advanced integration and calibration

• New IMS capabilities

• Automated instrument validation

• Reduces downtime and operating costs

• Fusion AE

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Varian 920-LC AIQ (Validation)

AIQ (Analytical Instrument Qualification)• Fully PC controlled IQ and OQ (not PQ)

• Puts the Varian 920-LC at the forefront of regulated capabilities

• Consistent, reproducible methodology• Includes pumps, autosampler, detectors, column

heater

• Chargeable option

• Fully documented

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Varian 920-LC Ordering Matrix

pump inject detector Oven Detector 2

option

Options

Modular Isocratic 210-LC 10mL Manual 325 PDA, RI, ELSD

Isocratic

00-100890-00Dual piston pump 10 mL head

Auto Dual UV/Vis

Opt FL, RI ELSD

Gradient

00-100891-00Quat, Grad, Degas Auto Dual

UV/VisOpt FL, RI ELSD opt

High Performance Gradient

00-100892-00

Quat, Grad, Degas Auto cooled Dual UV/Vis

Opt FL, RI ELSD opt

Development

00-100893-00Quat, Grad, Degas Auto PDA Std FL, RI ELSD opt

Research

00-100894-00Quat, Grad, Degas Auto cooled PDA Std FL, RI ELSD opt

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Varian 920-LC Target Markets

• Strong historical position for Varian in analytical

• Varian has the leading range of analytical technologies: spectroscopy, chromatography including consumables, data and services

• Target Users

• Environmental

• Industrial

• Generic Pharmaceutical *

• Academia and Biotech

• Same (but wider) target groups with a stronger product offering

*40% of Regulated Pharma has significant competitive barriers due to installed software base

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Varian 920-LC Silver Bullets

• Fast LC with superb performance optimised for sub 3 µm columns

– Improved throughput without compromising robustness

• Pulse-free solvent delivery– No need for a pulse damper

• High sensitivity detection• Full system level computer controlled AIQ (validation)

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Varian 920-LC Integrated Analytical HPLC