1: Tao of molecules

2. Scientific Approach

3. Code of life (Basepairer)

4. Transcription and Translation

5. Protein Folding

6. Hemoglobin and Sickle Cell

7. Mitosis and Meiosis

8. Punnett and Mendel

9. Pedigrees and Exceptions to Mendel

10. Unknown Liquids

11. Tools of the Light Trade

12. Colors of light and Waste Products

Photosynthesis and Electron Transport

System

Why we’re here:

• Solve a question about the ‘invisible world’ through experimental design and execution

• Design the experiment given tools you have and processes you’re familiar with

• Derive the purpose and implementation of ‘controls’ for your experiment

Where we’re going

• Given: DCPIP takes electrons from Pq

Will P-synthesis happen in DCPIP?

• How would you know?

• Add 0.2 ml/ml*final vol

• ALSO, confirm an herbicide (Diuron) is a photosynthicide (0.15 ml/ml*)

• Give your disks time to let the herbicide soak in

• *Ingore the change in volume adding these produce

Question of the day/week…

WHERE does the herbicide work?

X

X

The experiment’s afoot!• Hypothesis I: The herbicide (‘X’) acts before DCPIP has a chance to nab the electron

• Hypothesis II: The herbicide (‘X’) acts after DCPIP has a chance to nab the electron

Oh the thinks you can think!

• DCPIP taking -e will do what to O2 prod?

• How will -e taking affect DCPIP?

• How can you tell if drug acts here?

• Or here?

X

X

DCPIP taking e- will do what to O2 prod?

How will e- taking affect DCPIP?

How can you tell if drug acts here?

Or here?

Oh the thinks you can think!• Two hypotheses?

• What would your experiment look like?

• What would it TELL you if the DCPIP…

–Stays blue

–Turns clear

“Throw her into the pond!”• What tubes will you need?

• Work it out now

• Use the diagram

Evaluations

• What did you learn? (Can be done individually while experiments progress)

• What did you think of the semester (requires lab instructor to be finished & gone; but you can come back)