1

Exercise physiology and Exercise testing

Mitochondrial DNA and

Maximun Oxygen Consumption

2

Index

1. Introduction about this article2. General information

3. 1º investigation4. 2º investigation5. 3º investigation6. 4º investigation

7. Author’s opinion8. Final conclusion

3

Introduction about this article

1. This article is about relationship between mitochondrial DNA

and maximum oxygen consumption.

2. There are four different investigations.

3. The early researchers addressing the contribution of inheritance.

4

General information

DNA: Deoxyribonucleic acid (DNA) is a nucleic acid that contains the genetic

instructions used in the development and functioning of all known

living organisms and some viruses. The main role of DNA molecules

is the long-term storage of information

Different individual have different DNA sequence.Morphs may account for some of the

differences including VO2 max.

5

General information

Dna mitochondrial: (mtDNA) is the DNA located in

organelles called mitochondria.

Determinants of Maximal Oxygen Consumption:

1. Cardiac output2. The oxygen carrying capacity of the blood

3. The amount of exercising skeletal muscle and the ability of muscle to utilize supplied oxygen

6

1º investigation

Authors: Dionne et al.Aim: Association between mitochondrial DNA sequences and aerobic performance.

Also they assessed the relationship of baseline VO2 max and the response to training.Process: Endurance-training program

during 20 week.Subject: 46 North Americans who

were sedentary.Results: The subjects had a morph in the gene

encoding Subunit 5 of NADH dehydrogenase.

Conclusion: Relationship to the training responses of VO2 max.

7

2º investigation

Authors: Rivera and colleaguesAim: Measure the frequency of each of the

three morph identified within the NADH dehydrogenase and of one morph of the D-loop.

Subject: 125 elite endurance athletes and 65 sedentary.Results: Don’t found difference in the

frequency of these morph between groups

8

3º investigation

Authors:Chen et al, Ma et al.Aim: A researches focused on the D-loop,

which contains factors that the modulate mithocondrial DNA replications and transcription.

Subject: A sample of 120 Chinese subjects: 67 elite endurance athletes, 33 general endurance athletes, and 20 sedentary. Results: There were nine morphs, and their frequencies

were significantly different between three groups. Conclusions: This apparent association between mithocondrial

DNA D-loop polymorphism and endurance capacity needs confirmation. The authors suggested that a better relationship

might exist between the mithocondrial DNA morphs endurance performance rather than VO2max.

9

4º investigation

Authors: Melton et al.Aim: Relationship between mitochondrial DNA D-loop

morphs and VO2max.Subjects: 40 well-trained Australian male endurance cyclists.

Results: There wasn’t association between VO2max and the D-loop morphs in this population of athletes.

10

Author’s opinion

The autors are in agreement with reports of Dionne et al. (1991) and Rivera et al. (1998),

who found no significant relationship between D-loop morphs and either sedentary

VO2max or elite endurance athlete status.The discrepancy between these findings

and those of Chen et al. (2000) and Ma et al. (2000) may be related to the ethnic differences

between the subjects and the small sample sizes do not allow firm conclusions about the presence

or absence of small effects.

11

Final conclusion

There is evidence that V02max in athletes is

limited by the ability to deliver oxygen to the

muscles, rather than the ability of muscles.

However, mithocondrial function

could still be closely related to the

trainability of VO2max in previously

sedentary population and sub-VO2maximal

endurance performance in athletes.

12

JOSE MIGUEL PERUJO FRIAS

PEDRO ALONSO TEJERO SANTANA

THANK’S FOR YOUR ATTENTION