Lecture 5 Microbe Metabolism. Metabolism Metabolism: Metabolic Pathway:
1 Chapter 9 Metabolism: Energy Release and Conservation.
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Transcript of 1 Chapter 9 Metabolism: Energy Release and Conservation.
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Chapter 9
Metabolism: Energy Release and Conservation
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Sources of energy
•most microorganisms use one of three energy sources
•the sun•reduced organic compounds•reduced inorganic compounds
•the chemical energy obtained can be used to do work
Figure 9.1
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Chemoorganotrophic fueling processess
Figure 9.2
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Chemoorganic fueling processes-respiration
• Most respiration involves use of an electron transport chain
• aerobic respiration: final electron acceptor is oxygen
• anaerobic respiration– final electron acceptor is different exogenous NO3-,
SO42-, CO2, Fe3+ or SeO4
2-.– organic acceptors may also be used
• As electrons pass through the electron transport chain to the final electron acceptor, a proton motive force (PMF) is generated and used to synthesize ATP
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Chemoorganic fueling processes - fermentation
• Uses an endogenous electron acceptor
– usually an intermediate of the pathway e.g., pyruvate
• Does not involve the use of an electron transport chain nor the generation of a proton motive force
• ATP synthesized only by substrate-level phosphorylation
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Aerobic catabolism-An Overview
• Three-stage process– large molecules (polymers) small
molecules (monomers)
– oxidation of monomers to pyruvate
– oxidation of pyruvate by the tricarboxylic acid cycle (TCA cycle)
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manydifferentsubstrtaesare funneledinto the TCA cycle
ATP madeprimarilybyoxidativephosphory-lation
Figure 9.3
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Amphibolic Pathways
• Function both as catabolic and anabolic pathways
• Examples:– Embden-Meyerhof
pathway– pentose phosphate
pathway– tricarboxylic acid
(TCA) cycle
Figure 9.4
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The Breakdown of Glucose to Pyruvate
• Three common routes– Embden-Meyerhof pathway
– pentose phosphate pathway
– Entner-Doudoroff pathway
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The Embden-Meyerhof Pathway (glycolysis)
• Occurs in cytoplasmic matrix
• Oxidation of glucose to pyruvate can be divided in two stages
-glucose to fructose 1,6 -bisphosphate (6 carbon)
-fructose 1, 6-bisphosphate to pyruvate (two 3 carbon)
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Figure 9.5
•oxidation step – generates NADH
•ATP by substrate-levelphosphorylation
Glycolysis
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Summary of glycolysis
glucose
2 pyruvate
2ATP
2NADH + 2H+
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The Pentose Phosphate Pathway
• Can operate at same time as glycolytic pathway
• Operates aerobically or anaerobically an
• Amphibolic pathway
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•produceNADPH
•no ATP
•important intermediates
Figure 9.6
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Figure 9.7
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Summary of pentose phosphate pathway
glucose-6-P
6CO2
12NADPH
Glycolytic intermediates
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The Entner-Doudoroff Pathway
• yield per glucose molecule:– 1 ATP– 1 NADPH– 1 NADH
reactions ofglycolyticpathway
reactions ofpentosephosphatepathway
Figure 9.8
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The Tricarboxylic Acid Cycle
• Also called citric acid cycle and Kreb’s cycle
• Common in aerobic bacteria
• Anaerobes contain incomplete TCA cycle
• An Amphibolic pathway
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Figure 9.9
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Summary
• For each acetyl-CoA molecule oxidized, TCA cycle generates:– 2 molecules of CO2
– 3 molecules of NADH
– one FADH2
– one GTP
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Electron Transport and Oxidative Phosphorylation
• Only 4 ATPs are synthesized directly from oxidation of glucose to CO2 (by substrate-level phosphorylation)
• Most ATP made when NADH and FADH2 are
oxidized in electron transport chain (ETC)
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The Electron Transport Chain
• Series of electron carriers transfer electrons from NADH and FADH2 to a terminal electron acceptor
• Electrons flow from carriers with more negative E0 to carriers with more positive E0
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Electron transport chain…
• As electrons transferred, energy released
• In bacteria and archaea electron carriers are in located plasma membrane
• In eucaryotes the electron carriers are within the inner mitochrondrial membrane
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large difference inE0 of NADH andE0 of O2
large amount ofenergy released
Figure 9.10
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Mitochondrial ETC
electron transfer accompanied byproton movement across innermitochondrial membraneFigure 9.11
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Electron Transport Chain of E. coli
branched pathway
upper branch – stationary phase andlow aeration
lower branch – log phase and highaeration
Figure 9.12
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Oxidative Phosphorylation
Process by which ATP is synthesized as the result of electron transport driven by the oxidation of a chemical energy source
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Proton Motive Force
• Is the most widely accepted hypothesis to explain oxidative phosphorylation
– electron carriers are organized in the membrane such that protons move outside the membrane as electrons are transported down the chain
– proton expulsion results in the formation of a concentration gradient of protons and a charge gradient
– The combined chemical and electrical gradient (electro chemical ) across the membrane is the proton motive force (PMF)
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Chemiosmosis
Peter Mitchell in 1961 proposed that the electrochemical gradient (proton and pH) across a membrane is responsible for the ATP synthesis. He likened this process to osmosis, the diffusion of water across a membrane, which is why it is called chemiosmosis.
Peter Mitchell received the Nobel Prize in 1978 for this concept.
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PMF drives ATP synthesis(Chemiosmosis)
• Diffusion of protons back across membrane (down gradient) drives formation of ATP
• ATP synthase– enzyme that uses PMF down gradient
to catalyze ATP synthesis
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Figure 9.14 (a)
ATP Synthase
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Figure 9.14 (b)
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Inhibitors of ATP synthesis
• Blockers– inhibit flow of electrons through ETC
• Uncouplers– allow electron flow, but disconnect it from
oxidative phosphorylation– many allow movement of ions, including
protons, across membrane without activating ATP synthase
• destroys pH and ion gradients
– some may bind ATP synthase and inhibit its activity directly
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Maximum Theoretic ATP Yield from Aerobic Respiration
Figure 9.15
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Theoretical vs. Actual Yield of ATP
• Amount of ATP produced during aerobic and anaerobic respiration varies depending on growth conditions and nature of ETC
• Comparatively, anaerobic respiration yields fewer ATP that aerobic respiration
• In fermentation yileds very few ATP