1 AEL( pS )PVEQKL

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NSequenceANrt IMACrt SyntRatioNSequenceANrt IMACrt SyntRatio1AEL[pS]PVEQKLQ9Y5P860.155.61.144REIES[pS]PQYRLQ8WWM748.946.11.12REL[pS]PQRNLQ96F4437.135.31.145REY[pS]PPYAPQ9UPT851.348.01.13TER[pS]PGPRLO1497830.130.51.046KEKEV[pS]QELA8MX7633.933.31.04IES[pS]PQYRLQ8WWM752.949.81.147KEL[pS]PPPGLQ7Z4S662.557.21.15KEKDF[pS]PEALO1537251.147.61.148RER[pS]PSPSFP4958531.331.41.06GEW[pS]ASLPHRFO4316470.864.51.149GET[pS]LMRTLO9601770.764.51.17SEA[pS]PSREAIQ1311133.633.51.050AEK[pS]PGPIVQ86WB039.538.01.08YEG[pS]PIKVTLP0674872.466.21.151REN[pS]PVQSIQ7Z2Z135.735.01.09HERSI[pS]PLLQ9UBT657.353.11.152RET[pS]PNRIGLQ7Z46042.640.41.110VEKKKIR[pS]LO7539418.219.90.953REG[pS]FRVTTAP4975738.436.91.011AEN[pS]PTRQQFQ86XP332.833.01.054PEV[pS]PRPALQ96L7350.547.41.112QEV[pT]PSRSTLP4945438.537.41.055KEL[pS]PAGSIO9545346.544.01.113RE[pS]PPPSALQ86VE037.536.51.056REG[pS]YDRYLQ9HCD548.846.01.114REK[pT]PSEDKLQ8NFC620.622.40.957S[pS]YGNIRAVQ8N12247.945.41.115GEDR[pS]PPTALQ9BUB540.939.61.058GEPHS[pS]PELQ9NUA835.833.81.116REV[pS]PAPAVAO6029234.934.21.059REK[pS]PGRMLSTO1457827.628.21.017KEV[pT]PKPVLP1754443.040.81.160SEL[pS]PGRSVQ8NFF539.838.41.018HEL[pS]PPQKRMQ9BXP530.530.51.061HEFS[pS]PSHLQ9H3D439.838.11.019RE[pT]PSPRPMQ9UQ3526.727.71.062QEK[pS]PEERLQ7RTP626.427.51.020REYG[pS]PLKAQ9UQ8833.733.21.063KEQ[pS]PEPHLQ5VV5233.433.21.021KEM[pS]PTRQLQ4G0N733.933.31.064NEA[pS]PVKRPRLQ9H8U334.433.21.022GENK[pS]PLLLQ8IZD262.657.71.165G[pS]FSRFYSLP2269582.072.71.123VEFPH[pS]PEIQ9BR6168.062.01.166REF[pS]KEPELQ9NQS749.846.61.124YEKAN[pT]PELQ9H8V340.339.11.067SEKPV[pS]PKSGTLQ1415534.333.51.025VEK[pS]PRPPLQ7L59037.235.41.168REL[pS]PLISLP5182589.380.01.126QEK[pS]PKQALO4368322.224.00.969RE[pT]PVPPAMQ9Y2F528.929.81.027GEI[pS]PQREVQ8WWI141.840.11.070REP[pS]PSSSSLO6033634.834.11.028HEFH[pS]QENLQ9Y3M832.632.41.071LEKRT[pS]PQVLQ7Z41737.335.41.129REG[pS]GRFSLP5776446.543.91.172REL[pS]GTIKEIP3005054.951.01.130GEN[pS]GIGKLQ6P0N044.442.41.073GEA[pS]PSHIIQ9ULL5-351.247.91.131SEI[pS]PIKGSVQ7Z5K253.450.01.174GEQ[pS]PNVSLQ58WW250.047.71.032SEK[pS]LENETLP2358846.544.21.175KE[pT]PDKVELQ1361040.638.71.033RED[pS]QRPGAHLQ32P5124.025.30.976NEFH[pS]PIGLQ0641367.761.71.134RED[pS]GTFSLO7556959.655.41.177REV[pS]PEPIVQ8N10351.748.51.135REA[pS]PSRLSVO7512237.435.41.178NEK[pS]PYASLQ6IQ2145.043.11.036SEQ[pS]LGQKLP3399143.942.11.079REL[pS]GTIKEILP3005078.068.91.137REV[pS]PAPAVO6029236.335.21.080SERIMQL[pS]LQ81IY6383.273.81.138SERM[pS]LPLLQ1539891.281.71.181R[pS]FPTLPTLQ1376188.479.11.139SES[pS]PPKAMQ9UHB628.529.41.082KES[pS]PIPSPQ0108239.037.61.040SEV[pS]PSGVGFQ6ULP265.360.11.183SE[pS]GSNLHALQ6P4F743.842.01.041REK[pS]PGRMLO1457825.526.61.084KELARQI[pS]FQ9Y38554.250.31.142SEF[pS]DVDKLO7566570.363.81.185KEN[pT]PPALP3135038.737.41.043REA[pS]PLSSNKLQ9Y46240.238.31.0Supplementary data S2. MS2 spectra of the identified phosphopeptides. Each candidate spectrum is compared with the fragmentation spectrum of the indicated synthetic peptide. Red dots represent ion peaks that are not present in both spectra. The retention times of each natural peptide in the IMAC fraction (rt IMAC) and of the corresponding synthetic peptide (rt Sint) are shown. The ratio rt IMAC / rt Sint is also indicated.1AEL(pS)PVEQKLSyntheticPeptideCandidateSpectrum

2REL(pS)PQRNLSyntheticPeptideCandidateSpectrum

3TER(pS)PGPRLSyntheticPeptideCandidateSpectrum

4IES(pS)PQYRLSyntheticPeptideCandidateSpectrum

5KEKDF(pS)PEALSyntheticPeptideCandidateSpectrum

6GEW(pS)ASLPHRFSyntheticPeptideCandidateSpectrum

7SEA(pS)PSREAISyntheticPeptideCandidateSpectrum

8YEG(pS)PIKVTLSyntheticPeptideCandidateSpectrum

9HERSI(pS)PLLSyntheticPeptideCandidateSpectrum

10VEKKKIR(pS)LSyntheticPeptideCandidateSpectrum

11AEN(pS)PTRQQFSyntheticPeptideCandidateSpectrum

12QEV(pT)PSRSTLSyntheticPeptideCandidateSpectrum

13RE(pS)PPPSALSyntheticPeptideCandidateSpectrum

14REK(pT)PSEDKLSyntheticPeptideCandidateSpectrum

15GEDR(pS)PPTALSyntheticPeptideCandidateSpectrum

16REV(pS)PAPAVASyntheticPeptideCandidateSpectrum

17KEV(pT)PKPVLSyntheticPeptideCandidateSpectrum

18HEL(pS)PPQKRMSyntheticPeptideCandidateSpectrum

19RE(pT)PSPRPMSyntheticPeptideCandidateSpectrum

20REYG(pS)PLKASyntheticPeptideCandidateSpectrum

21KEM(pS)PTRQLSyntheticPeptideCandidateSpectrum

22GENK(pS)PLLLSyntheticPeptideCandidateSpectrum

23VEFPH(pS)PEISyntheticPeptideCandidateSpectrum

24YEKAN(pT)PELSyntheticPeptideCandidateSpectrum

25VEK(pS)PRPPLSyntheticPeptideCandidateSpectrum

26QEK(pS)PKQALSyntheticPeptideCandidateSpectrum

27GEI(pS)PQREVSyntheticPeptideCandidateSpectrum

28HEFH(pS)QENLSyntheticPeptideCandidateSpectrum

29REG(pS)GRFSLSyntheticPeptideCandidateSpectrum

30GEN(pS)GIGKLSyntheticPeptideCandidateSpectrum

31SEI(pS)PIKGSVSyntheticPeptideCandidateSpectrum

32SEK(pS)LENETLSyntheticPeptideCandidateSpectrum

33RED(pS)QRPGAHLSyntheticPeptideCandidateSpectrum

34RED(pS)GTFSLSyntheticPeptideCandidateSpectrum

35REA(pS)PSRLSVSyntheticPeptideCandidateSpectrum

36SEQ(pS)LGQKLSyntheticPeptideCandidateSpectrum

37REV(pS)PAPAVSyntheticPeptideCandidateSpectrum

38SERM(pS)LPLLSyntheticPeptideCandidateSpectrum

39SES(pS)PPKAMSyntheticPeptideCandidateSpectrum

40SEV(pS)PSGVGFSyntheticPeptideCandidateSpectrum

41REK(pS)PGRMLSyntheticPeptideCandidateSpectrum

42SEF(pS)DVDKLSyntheticPeptideCandidateSpectrum

43REA(pS)PLSSNKLSyntheticPeptideCandidateSpectrum

44REIES(pS)PQYRLSyntheticPeptideCandidateSpectrum

45REY(pS)PPYAPSyntheticPeptide

CandidateSpectrum

46KEKEV(pS)QELSyntheticPeptideCandidateSpectrum

47KEL(pS)PPPGLSyntheticPeptideCandidateSpectrum

48RER(pS)PSPSFSyntheticPeptideCandidateSpectrum

49GET(pS)LMRTLSyntheticPeptideCandidateSpectrum

50AEK(pS)PGPIVSyntheticPeptideCandidateSpectrum

51REN(pS)PVQSISyntheticPeptideCandidateSpectrum

52RET(pS)PNRIGLSyntheticPeptideCandidateSpectrum

53REG(pS)FRVTTASyntheticPeptideCandidateSpectrum

54PEV(pS)PRPALSyntheticPeptideCandidateSpectrum

55KEL(pS)PAGSISyntheticPeptideCandidateSpectrum

56REG(pS)YDRYLSyntheticPeptideCandidateSpectrum

57S(pS)YGNIRAVSyntheticPeptideCandidateSpectrum

58GEPHS(pS)PELSyntheticPeptideCandidateSpectrum

59REK(pS)PGRMLSTSyntheticPeptideCandidateSpectrum

60SEL(pS)PGRSVSyntheticPeptideCandidateSpectrum

61HEFS(pS)PSHLSyntheticPeptideCandidateSpectrum

62QEK(pS)PEERLSyntheticPeptideCandidateSpectrum

63KEQ(pS)PEPHLSyntheticPeptideCandidateSpectrum

64NEA(pS)PVKRPRLSyntheticPeptideCandidateSpectrum

65G(pS)FSRFYSLSyntheticPeptideCandidateSpectrum

66REF(pS)KEPELSyntheticPeptideCandidateSpectrum

67SEKPV(pS)PKSGTLSyntheticPeptideCandidateSpectrum

68REL(pS)PLISLSyntheticPeptideCandidateSpectrum

69RE(pT)PVPPAMSyntheticPeptideCandidateSpectrum

70REP(pS)PSSSSLSyntheticPeptideCandidateSpectrum

71LEKRT(pS)PQVLSyntheticPeptideCandidateSpectrum

72REL(pS)GTIKEISyntheticPeptideCandidateSpectrum

73GEA(pS)PSHIISyntheticPeptideCandidateSpectrum

74GEQ(pS)PNVSLSyntheticPeptideCandidateSpectrum

75KE(pT)PDKVELSyntheticPeptideCandidateSpectrum

76NEFH(pS)PIGLSyntheticPeptideCandidateSpectrum

77REV(pS)PEPIVSyntheticPeptideCandidateSpectrum

78NEK(pS)PYASLSyntheticPeptideCandidateSpectrum

79REL(pS)GTIKEILSyntheticPeptideCandidateSpectrum

80SERIMQL(pS)LSyntheticPeptideCandidateSpectrum

81R(pS)FPTLPTLSyntheticPeptideCandidateSpectrum

82KES(pS)PIPSPSyntheticPeptideCandidateSpectrum

83SE(pS)GSNLHALSyntheticPeptideCandidateSpectrum

84KELARQI(pS)FSyntheticPeptideCandidateSpectrum

85KEN(pT)PPALSyntheticPeptideCandidateSpectrum