University of Puerto Rico - Cayey RISE Program

Rubric for Assessing Workshop Summaries Rollbook# 5 Name Alejandra Ferrer Díaz Points

Date February 6, 2015 30 Points Total

Summary Number 1

1 2 3 4 5 A. PURPOSE OF THE LAB TECHNIQUE

Refers to purpose and objectives of the lab techniques

B. BIOLOGICAL COMPETENCE

Demonstrates knowledge of laboratory procedures

Reports findings adequately

C. ENGLISH COMPETENCE

Uses correct grammar, syntax, spelling, and punctuation

Demonstrates clarity and coherence

D. CRITICAL THINKING

In concluding identifies applications and or implications of the techniques

Template for 1ssh Workshop Summary Biol. 3955-Biomedical Techniques Due date February 6, 2015

1st Summary- Microscopy and Photo microscopy Dr. Robert Ross January 30, 2015

The workshop offered by Dr. Robert Ross about microscopy techniques had the following objectives: gaining knowledge

about different types of microscopy, kinds of micro techniques and being able to use and focus one of the light

microscopes located in the lab. In the microscopic world it exist two types of microscopy: light microscopy and electron

microscopy. The light microscopy can be subdivided in to four kinds: Bright field, dark field, Phase-contrast and

Nomarski. The bright field microscopy consists of light passing toward the specimen. On the contrary, dark field

microscopy has a dark background because the condenser moves and blocks light so it can focus on the specimen. In phase

contrast, the cells are amplified in a way that you can see the differences between them. Nomarski microscopy enhances

differences in density of cells but they’re seen as 3D. The SEM focuses on the surface of the specimen, while TEM on the

organelles and membranes. As part of a project, I’m working on viewing mushroom gills by using fluorescence

microscopy. I’m expected to see the basidia of the mushroom, the place where meiosis occurs. Finally, this workshop is

extremely useful to examine different parts of a specimen. It can be applied in a research or studies where the species that

serve as model are microscopic. By using these techniques, some discoveries have been made and what’s not visible to the

human eye can be comprehended.

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