01.).11 j 971 „Jr. L) L,a,,,v1

Assiut J. Agric. ScL, (48) No. (4) 2017 (121-131) Website: attp://www.nun.edu.eg/faculty_agriculture

ISSN: 1110-0486 E-mail: afas(k)aun.edu.eg

314.11 Commiphora sp. 6:41 &.4.44 414.41.1.1Ae. j:git Culex quinquefasciatus wickyt#11 ala Ficus nitida

J c ce.11.411 j

[email protected] ak44.):111:41S [email protected] AL I j. 11 ::41.c cu1.41 aortiT

Received on: 19/7/2017 Accepted for publication on: 9/8/2017

UA LISJ 4.1 j.4,,Nli vii.114,11 s 4.51..411 c).4 LIS . 9% A4-% Lflz Ficus nitida Commiphora sp..)4.11 L'..J*14'4 A 3101 Culex quinquefasciatus j 4rsL_.411 L..)1 A1.0tx..111 L).0 :tat. j V1,0 V j j jx4Il j,1.1 c.A3.5411 j

;Di 43,):14,11 j L).,d&a.„4.11 alaal .% 5111 .2c. 4.11.3:111 0 j._4.):311 4.11.3111 crIc. %1- A,AVi 1,Yr 71.57 L ).:0_92,,11 J.4.1 g.o _y4 %I. *L4.4.0.11 _):45'31-3 (LC50) c.).°.9'411 %0.L.44.411 a4.5-111 .%

:k--4_014.1.01.3 ;441411 JA.11 a4:3 (LC9o)L.J.431 L.J.4 % (fl,YV j 0,01) j(1,YA j ),Ir)i r,01 (t,rr ),v1) ,,,r1.31 F. nitida 01.).11 j_971 „Jr. % V u45..411 L),.-,,L,a,,,v1 .173 (a.1.:is,'1.411 Lji

cgi 9...:.111 LTIC. % j Y,Y A Loa LC90_, LC50 c4 j %1 f A j Fnitida c'Jt-41 01 J .,..;.111 ji ji5k.1

%s,11' , f f A4.,4 Lb..1) LsZI j f f 41411 L.)-1:,".--.11 LC90 j LC50 4.s." % 1 . 5A11 .11c. % 0 V,11/ LASi oacli 1446 j..g3._%:1431 zit:1:111 yip LJI i % 1 V, 9

• L')c. '17.1? ''.4.4.11144.1....0 JJ1.1.3 Lr_Lz ‘,:111 cJ.13

41.441-1 sJL;=,11 .Culex quinquefasciatus ,Ficus nitida. Commiphora sp..:i1--k 7-4 :44a1144 ZIALS.

olazi 111 - .4÷3.611 k4.4.4%'171 L:).4 j:oix411 Diptera A-__,4"1/1 A.._411,. 4.)*A..41 Culicidae :Lista L):03.411 cL4:1J- Okogun et al. :41.

, .11 JI Lgal>i ..›Les.11 1+3.4 jeai.)4tY C).4 1.1.13a .(2003 L:)1 t..6.)114 s .(Gratz and Jany1994) 4..;141 LJ4:11i 4114.3 4:313•14AV 11.4 ;L.4.•••AC :t 1" JAZ. .)4.0W CL.S. ‘..):1,4114 jC*)411

L.).01 .)4/ I ..)13& Lst.k) f.ILidl 03„, ‘,61.1.4 L).4.! .(Fardin and Day2002) j c.51.11 4'4 ').4 Phytochemicals

j1.1. 0=1.).4 1.44.3 I j ,D1 L:AAJ C:11

j j,t,

r cAill ric. V-114// http://ajasjs.iknito,conil

Babu and Maruugan 1998; Venketachalam and Jebasan 2001 a&b; Mittal )

and Subbarao 2003; Bagavan et al. 2008; Ghosh et al. 2008; Kannathasan

cL1 (et al. 2008; Rahuman et a/.2008; Senthilkumar et al. 2008.

k,4t.41 4:111i

bl Cryptomeria japonica c:*.1, j L.3.1 jji

Aedesaegpti (Diptera: Culicidae) (Cheng et al. Loitc. 4

Thermogenic j j j j+i s.1.4 .2003)

.)-1....3 anodyne j j anthelmintic j j j

Jj_411 j haematinic jemmenagogue j j demulcent j

.(Warrier et al 1993) j (Husain et al 1992) lithontroptic

L; ...j.4—.L.N1) jJ3: L L,S431i .:.4431 j

LL.43I j41.1 .4-1.1301 :Asc, .1).311 LL.'51.).:Azil .1.1=

Rhinacanthusnasutusi 0.sancturn j Ocimumcanum,::A:41 (31)j1

LIS JUI C. quinquefasciatus 9 Aedeseagypti t41.)11

Morimoto et al. 2002; koul et a/.2003; Rahuman et al. 2008; )

L.„1■ (Kamaraj et a/.2008; Amer and Mehlhorn, H. 2006 a&b

R. 3 canum Ls jivl L j1 1.A5 .4.FAS nasutus

,;=33A4 3 A. Aegyptij;.j..4 L.4.1c, 0. sanctum k.

Movaceae k1sW■ t1,0 ,:**)41,1 .C. quinquefaciatus

),1 d,,,,,;,, Ficusvirgata 41.

Singh L.)--A .(Konno et al. 2004) L.) 2005

s J.1-2 Ficusbenghalensis s Saratchandra

.. Dron j Alexenizer

(Oncopella fasciatus) •1k.e.--13 .H3=cii' j 1,4 .)S0 4-1 Ficuscarica

L.):. utsy c:J► Jka.► .Milkweed bug

Pathak et al. 2000, Sun ,,f,-14j j.41■ JAS:J a-,11,:LA:31.4111

et al. 2001, Singh et al. 2002, Sivagnanaame and Kalyansundaram 2004,

,...).j.).>■3 Abdul Rahman —3 .0bomanu et al. 2006; Singh et al 2006

Ficusracemosa ja." A ?lam

Anopheles L-43.A.A.11 Aedesaegypti L.y44,1 t4,)11 41. _)111 jilt)1 3-36

2009 j v..51)* j .C.quinquefascialus stephensi .c.

LFI ja,LA Schangini aegyptiaca t43.3.3.1■ L'.51 -,<€11

4.1c- j • j-11 _)?41103. '1' .34 _A .1a. C. quinquefasciatus .3.41 kklt.41■

4:12tAda % 100 L'-• C. pipiens L):=Iyy 2009

50 5,11 L.91 .).9\ .9'49

p % 97.1 ,:zAS 0L. 511/.5411..

;A-4 % 100

Azadirachta excels a ,:).4 .c.5.1.31 L521vls Il C.Y2► L.1-d'

122

Assiut J. Agric. Sci., (48) No. (4) 2017 (121-131) ISSN: 1110-0486 Website: http://www.aun.edu.eg/faculty_agriculture E-mail: [email protected]

Quercus infectoria DL. Cleome glaucescens Dc. Jack M_› .).C.4.4. 200 j4S. %100 :4,44 C.pipiens

40 j....45.111 c:.%)11)41 )41.1:J. A. Excelsaj1j31

L..) (Y 0 ) LI) )► .v, .(Mustafa and AI-Khazraji 2008) jai' M y. j t:.3.5:MA :511 Tamarixr amosissima 3t LIA4.).011

,LJ1k.}1, }:gy.01 Jr)U 40-% C. pipienes j:3.411 j.t0-%3 :UP ..}A j►.312V1 ul 0 • 1 £ C.J1.1.4 j3 1.11 Cya L14/ m.,%1.4 .}6 .11c. % 9 • Ls) am.11.)

j:zt-mq L Cordimyx u1 .1 jup :43.341:11 )-4s-"t.4 1.„10"),41 )1 31:1/1 A J..) C. pipiens

4.:011 45.1 3 0...3_3041 Lilo 441.,31 A Lsk• .;411.2-11 A..4L.:10) c.111.s.S.).011

J AI )a•'(•:-1-111 ly L:14 11 J-C1 (Ranson et al. 2001) 14..41.1&a.V j4.A.11 1:110 kii..1...31 L.41).111 01.1.01.) 4s1Gt 9 j.tSi ,DA

3.4 j Lck. .1114

zia431 Jo. A...1.1+C.111 01,1 J349 1 9 3-4111 ic 14.1 j:ema.,11 .(Sivagnanaame and Kalyansundaram 2004) :II

4.)11 ./4y11 )111.vi

tg4.44,0i ;4.).1. 1 )1.31.'1'1 u-1a C.ra kia.111 El-Hag et al. 2001; )

-;II 143 .(Dharmagadda et al. 2005 Ficus nitida 01 Commiphora spp.

. Culex quinquefasciatusL):..94

Ss1 al.gda

:4114.111 c:Jt -4 ')LS r1.1&:a.' Jr- c),S1.3411 11146.411

jcd:Nie,' 143 1.1*:11 L').4 j..c&.N.11 r.3 4.1113 Commiphora sp. - ;■z.►)• ).11 Ficus nitida ►413:

01-411 &.1.:Nal4 1.431 v y as :41.S13 aiL.L111 43 c:J

X91 . S 13 J411 gr-11-.13 t--"° C.)4 (a.341 J.33 .-4...11.3 .3,*-,1) 3.4 0 . ‘1*.lx...:033 N"%:+..K.:011

C3)3.1.-.11 ‘=65-' o1a L5 JS LI)454.11 9 7314411 L.) 1 °1-411 L).4 JS L.,)4 L;,4

Lsic. 0.1.,1(KS500, JANKE & KUNKEL IKA -WERK) -11 410. c.11 • •;kr.). LrIc. °25±2C 3.1 ):&.,-.1°25±2C 4).1 LfIc. `1 y.,3 Whattman No.1 cr.,'_;.)

0,,LS11 jya,.%11.3 4cL.,a f A 4141 ..).1014.1. 1 0.1171 L:).4 43 ,11 .1,I4 .4.413.31 L);es jm.,1

o:L.A Lla 10.14:10‘421 L:31_34 jai' 410 Stock solution

123

j„, -1 1Li http../jajas.js.iknito.com/ •

c jI j p1 j-411 JA :sk.S3 % , AiL;:3) 1i Ficus nitida

LLL...11.:111 j-4x.31) 1 • j j.5.4 JS j JS _y5.4 L:).4 A ji ot.4.11 .141.2 j.)_ca A.A....41q i(t..)1.)113

.A:4431 li

:0:09241 :t4i) 11 :k115.? jAx.4 .a j:03.x.411 ;171.).51

j.c.x.411 cs_11 i‘ri emA1)1 04411 j.:01..pi L.).:114,111 Lrla jiL111) 11f111 j.4.L11 Ls1) kisaNø 1 . 1

C. ti--1111-4)1 diarbach 1988 • L:j j-&1.3 jya t..31 jj u 54.e.A; • quinquefasciatus

c-J 4.431 :911"

J.45.11 L1,324.L. CRD j A 31.1....71 SAS :L12.1.".? (LSD),....'31. 4.3:111.3 L.31.3..

L3.4

% _.3 _34. 5331 j (LC50) (.3.4 °/00 • .345111

. LDP z...4)44(1...C9o) :

iS1 j 3V1 j 44, '.144113 Ficus nitida L :31 j 13 Commiphorasp.

LDI Lt e.j1 .).‘",11 e41.)..11.53t3I cci)111 JAL11 C. quinquefasciatus 431 LJ.3 -43 4.1,,y4i j j j 41*.411

1,13 ,0/0 ) . 5,11 crILi.111 %1. 3 V 1 , V cl • al • L);c235L.41 )eij1.1

%j1.11 J. &AAJ Ai Ls21,33 4431 j 43l L\•

L4-C . 431 j..1. ] Lsk- CY01 A , AV j ,vr \),V °/01.,•% 411L4.11

.() Li_94) il Lric- % Y S.LAII t. 3:4' 1 ;1.1426011 0:g1 ja.41 c..14.41a.,1141

Commi hora s . 4441 cA;41,9 431,9 :-ILI,A cj:akyL#11 ‘'..'a J.41 A.4.1411 4.4Zil we 1.111

0171.4* yl...9 Cr % ..54s..5111

‘,...L•41 ,19.1....n c...)-:-;#11 C)-.9:1:-,4: VI J-9•1'44•411 A.)11 60.00 A (g) 90.00 A (a) 71.57 A (b) 90.00 A (a) 2.50 A (r) ■ .

37.47 B (h) 68.87 B (d) 69.73 B (c) 71.57 B (b) 2.50 A (r) ° 34.45 C (j) 60.67 C (f) 30.66 C (1) 64.16 C (e) 2.50 A (r) r 21.56 D (n) 26.57 D (m) 35.97 D (i) 21.22 D (o) 2.50 A (r) ■

15.00 E(q) 18.43 E(p) 31.11 E (k) 2.50 E (r) 2.50 A (r) • .0

5.51 1.63 7.0072

1.97 I 1.76 LSD LC50

46.27 6.28 13.59 4.33 LC90

124

Assiut J. Agric. ScL, (48) No. (4) 2017 (121-131) ISSN: 1110-0486 Website: http://www.aun.edu.eg/faculty agriculture [email protected]

• 914.0 )l.41 e4.44 1%1,11

L):09_341 Lsi.c. ;,i1"+:11 Jc.t: ctIcl:1111 c.:J:1/414 LAS

9 (LC 5 0) YL-111 (:).4 %o _3 ..St1111 c.2.14). j111 1.)t-?. ...)-.3 164

‘:_*)1.4:) (LC90) c›;°_93‘411 <rai)) Lj.4 °A39 . c41.4.411 .j1.

1,11 9(11",09 9 ),,ty) j (t,rr 9 ),v1) N1i 4#1,11°3.4.,

.u4s.m. .t411 ..rte Ji4). 4.11;►1 (fl,YV j 0,01)

C.91 .).917 LI.,:3141•431.9 t.it'"(611 9 93=11 Ocimum canum, Ocimum sanctum, Rhinacanthus nasutus

j: p'`Ua' j .C. quinquefasciatus Aedeseagypti _›.11

0. 444-.N1 R. nasutus,, 0 canum Lg1934.11

(LC50 = 99.42, 94.43, 81.56% ) A. aegypti La's' Lslasanctum Lria (LC50 = 44.36, 73.40, 38.30%) C. quinquefaciatus

(Morimoto et al. 2002; koul et al. 2003; Mehlhorn and Amer 2006 zet-al-11&,.. 'LOA .),L).'`UI ►a,ti a&b; Rahuman et al. 2008a, Kamaraj et a1.2008). L.5.1 4 ~~.̀~t:i~11 l■I-L=3 J.411 6.4AGI LLII • /■1■ .■ 4.4

* v ) L.>°941 ‘.43.4 Lsizi -1.,:)4.11 Ficus nitida L1:►1 ..4,5.1? ji4 4.11i.111 Lolc. % o j .11c.

.% • 5111 ,.1_1c. %ro, Y t 0:.y,411 19 1 uy4 4s..0 loll j-14-1-11 lic..1 LoS

LrIc. tA.),1.3L L41.S .1, *)L4.11_,

:t11..UA ;Ials..411 oekga411 4.0AI 4.0.111 . r jj4 vAYI 414 Ficus nitida c:11,1:1 djj‘i j,04411...9

v",--:‘;2,9411,-W.A au-4 ;k0.6,1 4-111 .14=1 012,14 c)

.;3S.9:111 J.96-411 C>4 %

t (.>1...)41 C)-9:1+41ti / J.911'1+41 FLAIL

13.18 A (h) 15.00 A (e) 50.07 A (a) 35.24 A (c) 2.50 A (1) t .

12.92 B (j) 13.94 B (g) 39.23 B (b) 23.11 B (d) 2.50 A (1) °

2.50 C (1) 7.49 C (k) 24.12 C (g) 14.06 C (t) 2.50 A (1) .c:.

2.50 C (1) 2.50 D (1) 2.50 D (1) 2.50 D (1) 2.50 A (1) t

2.50 C (1) 2.50 D (1) 2.50 D (1) 2.50 D (1) 2.50 A (1) ..0

4.628 LSD

2490.69 803.34 7.28 18.79 LC50

20753.58 37153.45 28.26 105.14 LC90 .. ,,

Jim-4 ) Nip A...LOLA .1

(LC50) j:=2y41 Clay t j.a %0 .L_14.4411 j.s..S.5111 L ji c2:1111 LS.)&1 L:).4

.:.11L4:11 Lj..alii...al (LC90) J.Lass9141 Lj.4 %'l . j451.11

3 \t, t' A) L ').4 1A19.4 Loc. _5451 LOD 4.in 4.33314431 Ficus nitida

125

hup: //ajus js .iknito,com/ . 1 ,Abl Lc4r1c. Jj.4. o cry U.I1 LIANia ;Ls..

;;, 411.3 ` LDSI.L.-.131 A.111 L.14.43.iJ to e c11111 e 3A. . Y J9.Li (Y A,

R. nasutusi 0. Canum ca4:1--1,33:) Lii:) 4L-14-414

Ljk-Si C. quinquefasciatus 4ticyy a.a 0. sanctum --I .(Kamarj, et al., 2008) 4315:13. 1 ala LC50% 44.36, 73.40, 38.30

Ficus nitida L:51 j..;*11 L.) r :,14:1D L,J1 Lsj A

41 ot~e o11 L):0 j.41 LIU j 43j:44.4,11 L.›.ca&a.4.411 % .)71.3 5111 SIG % V

L 4.11 Lsk. % Y, o j 0 j j):IS. 5.111 ulaC, °A:ItY,1A j j 0Y,1■/

cz19 % 3.3..;11. .11Z. 41,41.14111 L):03.41 C.A):11 L"..) Jr* % f • 4Ltoa11

CS.)--"? ~ ,etl )1113 (31 11..5 / ‘'Ll/ t 1A' (Yo r L);=3.Y.41i (L.). ti)43 c43...11 (LC50) j:..341 ,..d19* ,_).4 C j.o %o • _I LDS ,LID

.01 L-31..)3"D j-14----1 (LC90) %‘\ • ..J j1 t, • t) Uie. 44.4.A11 3 41,1-.11 Ficus nitida

.rJj41 4...k5;111 ls1c %() A,\O' j r,v0) Y f 9

14,1 o &--.4 ZL41.11.441. L;0 y411 C.ItkAl. Z.19A3 .4.&$.14.11 4.111 .16 J94 t A .124 Ficus nitida zA4:4 j1jjat al,;#119 cjj:1.1.4, JJA6411J

-)Ail ‘ .1141 ‘:11 "'‘W''': L);=3.P431 ,L1.11.)71. ,L1',41 ;ti9141i 4,,131 .1.4aLL.111 ()AA MA)

.34;41.1 .sa

4,.w1A4:r.

1 % Cr.-34 C..94. 1 J.A.11 plAll

22.79 A (f) 21.39 A (g) 57.17 A (b) 72.44 A (a) 2.50 A (n) ∎ •

18.63 B (k) 20.00 B (j) 50.13 B (c) 40.40 B (e) 2.50 A (n) 0

14.30 C (1) 10.14 C (m) 47.18 C (d) 29.93 C (f) 2.50 A (n) '.c>

2.50 D (n) 2.50 D (n) 20.53 D (h) 28.73 D (g) 2.50 A (n) )

2.50 D (n) 2.50 D (n) 2.50E (n) 2.50E (n) 2.50 A (n) • .°

6.936 LSD

84.77 85.89 3.75 4.04 LCso

1337.55 1225.64 18.72 17.49 LC90 j1.41,4„,

■);;.I'LL J:i'D Ficus nitida c..*-114:J j .3'11 L, I9 444211 1 LDIS3 Abdul ZG-S 4:14 ,it."LI C. q u in q uefas c ia t us

i....f11 1 j . Rahman et al. (2008) L).;.y411,La.);11, t.11 ,)1I Litati j..S Ficus racemosa cr.111 (%192.77 9 106.50 3 LC90 64.99) (%41.423 28.503 14.55) LC50 C..)‘-S

Anopheles stephensi 1.--LP-43► Aedesaegypti L'ya J.C3

:) 1---411 441. t-4 1lA z=31:1331 014 cA.613:!1-45 031_9:111 Lric Culex quinquefasciatus Ficus nitida L31 k,x) :4,:t...1►

126

Assiut J. Agric. ScL, (48) No. (4) 2017 (121-131) ISSN: 1110-0486 Website: http://www.aun.edu.eg/faculty agriculture E-mail: [email protected]

;L-lax.11 tl U.5 .C.quinquefasciatus 1:,1", 4 L+10 L...J1 syt *.-11 J.L29.11 1+1 JI9a 14.4 Movaceae

1.,s_xaa j L11.1:.= Ficusvirgata Ficus ( .31 jiti j 541 j-k.',—.11 .1.= 43 (Konno et al., 2004) Arannilewa et al., ) Sitophiluszeamais exasperate (Singh -0.41 „lc. o,JS11 1+1 Ficus benghalensis s wt d v9 1....S (2006 -0.).1-.111 L.:.?.1Ficuscarica and Saratchandra 2005)

Milkweed bug, Oncopella fasciatus j J .1)) aj s.gil9 Govindarajan .(Alexenizerand Dron 2007) Ficus j Ficusnitida J. j:A.-41.411 j V yl9 a, j:411 Culex ‘2.1.111_}1 benghalensis LC90 j LC 50 Le jt.--S . Anopheles subpictus tritaeniorhynchus

.4.11.3:111 Lsiz ppm Ao,At j 01,11 j PPm 109,Y1 j .,AA

:tTh4V1 .3 :t7Y—)2.31 Lu1 y..11 ,(V • 1 0) j_a_5.4 j ‘Lsi 9.411 j

Tamarixr amosissima 20).11 A+•;3J.111 0..„1&:1," 49 JI j 1-11 L1:4 Culex pipienes

.r • f: .cr r

i) JALS (:)143.i LAL1 .<11 012171 i Cord iamyxa ‘L.-31-41

1.t"..4.4146 ah.o .(Diptera: Culicidae Culicinia) Culexpipienes . rn_v.rr : vr 4„11 A .)a.,LD

"t-434131 1' " c)°--4) c ) •e, • 44.9i1c'L-11 't 441C' "Lin%az Culex pipiens )46 „Jr- (Neemix4.5)

.) r :(r) o .....41,„11 1,14,31 Luz .0 LI 4.4 ji 44*AL). Cj19.`0) LS c4..S1.44c

Culex spp. (Culicidae : Diptera)

.1v Y_ t 11' r: f . jla (.31 j̀ i cjt <€11 rws..") CC • .4.)1 Culex Schangini aegyptica

quinquefasciatus (diptera:Culicidae) Say. :())

Abdul Rahuman A.; Venkatesan, P.; Geetha K.; Gopalakrishnan G.; Bagavan, A. and Kamaraj, C. (2008). Mosquito larvicidal activity of gluanol acetate, a tetracyclic triterpenes derived from Ficusracemosa Linn. Parasitol Res.103:333-339.

127

htip://ajasjs.iknito.com/ r. vJG

Alexenizer M, Dorn A (2007). Screening of medicinal and ornamental plants for insecticidal and growth regulating activity. J. Pest Sci 80:205-215.

Amer, A. and Mehlhorn H. (2006a). Larvicidal effects of various essential oils against Aedes, Anopheles, and Culex larvae (Diptera. Culicidae). Parasitol Res 99:466-472.

Amer, A. and Mehlhorn H.(2006b). Persistency of larvicidal effects of plant oil extracts under different storage conditions. Parasitol Res 99:473 477.

Arannilewa ST, Ekrakene T, Akinneye JO (2006). Laboratory evaluation of four medicinal plants as protectants against the maize weevil, Sitophiluszeamais (Mots). Afr. J. Biotechnol 5:2032-2036.

Babu R., Murugan K. (1998). Interactive effect of neem seed kernel and neem gum extract on the control of Culex quinquefasciatus Say. Neem Newsl

15(2):9-11. Bagavan A, Rahuman AA, Kamaraj C and Geetha K (2008). Larvicidal activity

of saponin from Achyranthesaspera against Aedesaegypti and Culex

quinquefasciatus (Diptera: Culicidae). Parasitol Res 103(1):223-229. Cheng S.S, Chang HT, Chang ST, Tsai KH, and Chen WJ (2003). Bioactivity of

selected plant essential oils against the yellow fever mosquito Aedesaegypti larvae. BioresTechnol 89(1):99- 102.

Dharmagadda, V.S.; Naik, S.N.; Mittal, P.K.; Vasudevan, P. (2005). Larviciadalactivity of Tayetespattulaessential oil against three mosquito species. Biosource Technol.. 96,1235-1240.

El-Hag, E.A.; Abd-ElTahman, 0.; El-Nadi, H.; Zaitoon, A.A. (2001). Effects of Methanolic Extracts of Neem Seeds on Egg Hatchability and Larval Development of Culex pipiens Mosquitoes. Indian Vet.J. 78,199-201.

Fradin MS, Day JF (2002). Comparative efficacy of insect repellents against mosquito bite. New Eng J Med 347(1):13-18.

Ghosh A., Chowdhury N., and Chandra G. (2008). Laboratory evaluation of a phytosteroid compound of mature leaves of Day Jasmine (Solanaceae: So-lanales) against larvae of Culex quinquefasciatus (Diptera: Culicidae) and nontarget organisms. Parasitol Res 103(2):271-277.

Goddard L.B.; Roth A.E.; Reisen W.K. and Scott T.W. (2003). Vertical of West Nile virus by three California Culex (Diptera: Culicidae) species. Journal of

Medical Entomology 40: 743-746. Gratz NG, Jany WC (1994). What role for insecticides in vector control

programs? Am J Trop Med Hyg 50(6 Suppl):11-20. Govindarajan, M.; Sivakumar, R.; Amsath, A. and Niraimathi, S. (2011).

Mosquito larvicidal properties of Ficus benghalensis L. (Family: Moraceae)

against Culex tritaeniorhynchus Giles and Anopheles subpictusGrassi (Diptera: Culicidae). Asian Pacific Journal of Tropical Medicine. 505 - 509.

128

Assiut J. Agric. Sc!., (48) No. (4) 2017 (121-131) ISSN: 1110-0486 Website: http://www.aun.edu.eg/faculty_agriculture E-mail: [email protected]

Harbach, R, E. (1988). The Mosquitoes of the Subgenus Culex in Southwestern Asia and Egypt (Diptera: Culicidae). Contribution of the American Ento-mological Institute. 24,(1).

Husain, A., Virmani, 0. P., Popli, S. P., Misra, L. N., Gupta, M. M., Srivastava, G. N. Abraham, Z. and Singh, A. K. (1992). Dictionary of Indian Medicinal Plants. CIMAP, Lucknow, India. 546p.

Kamaraj, C.; Abdul Rahuman, A. and Bagavan, A.(2008). Antifeedant and larvicidal effects of plant extracts against Spodopteralitura (F.), Aedesaegypti L. and Culex quinquefasciatus Say. Parasitol Res. 103:325-331

Kannathasan K, Senthilkumar A, Venkatesalu V, and Chandrasekaran M (2008). Larvicidal activity of fatty acid methyl esters of Vitex species against Culex quinquefasciatus. Parasitol Res 103(4):999-1001.

Konno K, Hirayama C, Nakamura M, Tateishi K, Tamura Y, Hattori M,and Kohno K (2004). Papain protects papaya trees from herbivorous insects: role of cysteine proteases in latex. Plant J 37:370-378.

Koul 0, Daniewski WM, Multani JS, Gumulka M, and Singh G (2003). Antifeedants effects of the Limonoids from Entandrophragmacandolei (Meliaceae) on the gram pod borer, Helicoverpaarmigera (Lepidoptera: Noctuidae). J Agric Food Chem 51:7271-7275.

Mittal PK, and Subbarao SK (2003). Prospects in using herbal products in the control of mosquito vectors. ICMR Bulletin 33(1):1-12.

Morimoto M., Tanimoto K., Sakatani A., and Komai K. (2002). Antifeedant activity of an anthraquinone aldehyde in Galiumaparine L. against Spodopteralitura F. Phytochemistry 60:163-166.

Mustafa, M. A. and Al-Khazraji, A. (2008). Effect of some plant extracts on the Culex pipiensmulestus Forskal. larvae. Iraq. J. Vet. Sci., 22(1) 9-12.

Obomanu F.G.; Ogbalu O.K.; Gabriel U.U.; Fekarurhobo G.K. and Adediran B. I. (2006). Larvicidal properties of Lepidagathisalopecuroides and Azadirachta indica on Anopheles gambiae and Culex quinquefasciatus. Afr. J. Biotechnol. 5 (9): 761-765.

Okogun G.R.; Nwoke B.; Okere A.; Anosike J. and Esekhegbe A. (2003). Epideminological implications of preferences of breeding sites of mosquito species in Midwestern Nigeria. AnnAgric Environ med.;10 (2): 217-222.

Pathak N.; Mittal P.K.; Singh 0.P.; Vidya Sagar and Vasudevan P. (2000). Larvicidal action of essential oils from plants against the vector mosquitoes Anopheles stephensi (Liston), Culex quinquefasciatus (Say) and Aedesaegypti (L). Int. Pest Cont. 42: 53-58.

Rahuman A.A, Gopalakrishnan G, Venkatesan P, Geetha K (2008). Larvicidal activity of some Euphorbiaceae plant extracts against Aedesaegypti and Culex quinquefasciatus (Diptera: Culicidae). Parasitol Res 102:867-873.

Ranson H.;Rossiter L.; Ortelli F.; Jensen B.; Wang X.; Roth C.W., Collins F.H. and Hemingway J. (2001). Identification of a novel class of insect glu-

129

http://gjasjs iknito.conil r . I/ 64.01 4.1c. 4"..tc i4fisti..11 j

tathione S-transferases involved in resistance to DDT in the malaria vector Anopheles gambiae. Biochemical Journal.; 359:295-304.

Senthilkumar A, Kannathasan K, and Venkatesalu V (2008). Chemical constitu-ents and larvicidal property of the essential oil of Blumeamollis (D. Don) Merr against Culex quinquefasciatus. Parasitol Res 103(4):959-962.

Singh R.K.; Dhiman R.C. and Mittal P.K. (2006). Mosquito larvicidalproperties of Momordica charantia Linn (Family: Cucurbitaceae), J. Vect. Borne. Dis.

43: 88-91. Singh RN, and Saratchandra B (2005). The development of botanical products

with special reference to seri-ecosystem. Caspian J Env Sci 3:1-8. Singh S.P.; Raghavendra K.; Singh, R.K. and Subbarao S.K. (2002). Studies on

larvicidal properties of leaf extract of Solanum nigrum Linn. (Family: Solanaceae). Curr. Sci. 81: 1529-1536.

Sivagnaname N. and Kalyanasundaram, M. (2004). Laboratory evaluation of methanolic extract of Atlantia monophylla (Family: Rutaceae) against immature stages of mosquitoes and non-target organisams. Mem. Inst. Oswaldo Cruz, Rio de Janeiro. 99 (1): 115-118.

Sun R.; Sacalis J.N.; Chin C.K. and Still C.C. (2001). Bioactive aromatic compounds from leaves and sterns of Vanilla fragrans, J. Agric. Food

Chem.. 49: 5161. Venketachalam MR, Jebasan A (2001a). Repellent activity of Ferronia

elephantum Corr. (Rutaceae) leaf extract against Aedes aegypti. Biores

Technol 76(3):287-288. Warrier, P. K., Nambiar, V. P. K. and Ramankutty, C. (1993-1995). Indian

Medicinal Plants. Orient Longman Ltd., Madras. Vol. 1-5. Zinser, M.; Ramberg, F. and Willot, E. (2004). Scientific note: Culex

quinquefasciatus (Diptera: Culicidae) as a potential West Nile virus vector in Tucson, Arizona: Blood meal analysis indicates feeding on both humans and birds. 3pp. Journal of Insect cience, 4:20.

130

Assiut J. Agric. Sci., (48) No. (4) 2017 (121-131) ISSN: 1110-0486 Website: http://www.aun.edu.eg/faculty agriculture E-mail: [email protected]

The Effect of Extracts of Commiphora sp. gum and Ficus nitida leaves on Larval Mortality of Culex quinquefasciatus under Laboratory

Conditions

Khalid, SaeedAqlannaserl and El-moflehi, Mahmoud Ali Abdallah2

'Department of Biology, Faculty of Education-Thamar, Thamar University. kh.

2Department of Plant Protection, Faculty of Agriculture, Sana'a University, Yemen

Abstract: This study was conducted on the effect of the extracts water, methanol, ace-

tone and benzene for each Commiphora sp. gum plant and leaves of Ficus nitida plant on the larval mortality of Culex quinquefasciatus under laboratory condi-tions. The data was recorded after 48 hours of treatments. The results showed that the percentages of larval mortality of Culex quinquefasciatus with the water, methanol, acetone and benzene extracts of Commiphora sp. were 90, 90, 71.57 and 60%, at 10%, respectively. However, water, methanol and acetone extracts of Commiphora sp. gave 71.57, 69.73 and 68.87% larval mortality of C. quinque-fasciatus, at 5%, respectively. The LC50 and LC90 of the water, methanol, acetone and benzene extracts of Commiphora sp. were (1.7 and 4.33), (1.97 and 13.58), (1.63 and 6.28) and (5.51 and 46.27), %, respectively. Methanol extract of F.nitida leaves gave the highest percentage of larval mortality of C.quinquefasciatus, 50.07% at 10% after immediate extraction and the LC50 and LC90 values were 7.28 and 28.26%, respectively. The results showed that the wa-ter extract of F.nitida leaves, was used after 48hs of extraction, gave the highest mortality percentage of C.quinquefasciatus Larvae; 72.44%, followed by Metha-nol extract with the mortality percentage of 57.17%, at 10 %. The LC50 and LC90 values of water extract were 4.04 and 17.49%, respectively. These results were considered promising and viable for further studies to be pursued pertaining to the study on bioactive plants which represent an environmentally sound alterna-tive for synthetic pesticides. Keywords: Extracts, Commiphora sp. gum, Ficus nitidaleaves, larval mortality, Culex quinquefasciatus

131

01.).11 j 971 „Jr. L) L,a,,,v1

Documents

Transcript of 01.).11 j 971 „Jr. L) L,a,,,v1