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1Microbial Gene Research & Resources Facility (MGRRF)Institute for Molecular & Cellular Therapies (IMCT)Biomolecular & Biomedical Sciences (BBS)
New Lorne Bore (RN 17263). Depth 1613 m, Temp: 89 oC, pH 8.5); Flow rate: 7.56 L/sec; Chemistry: typical Na-HCO3-Cl
Brisbane
Sampling at bore source
Sampling below bore source
2Microbial Gene Research & Resources Facility (MGRRF)Institute for Molecular & Cellular Therapies (IMCT)Biomolecular & Biomedical Sciences (BBS)
The New Lorne Bore (RN 17263) runoff channel
Temperature specific microbial colonisation occurs in the New Lorne bore run off channel in a temp gradient (88 oC to ambient)
Sampling microbial
mats
3Microbial Gene Research & Resources Facility (MGRRF)Institute for Molecular & Cellular Therapies (IMCT)Biomolecular & Biomedical Sciences (BBS)
Grey Filaments at 75ºC Red Filaments at 66ºC
Brown Filaments at 52ºCGreen Filaments at 57ºC4 spatially distinct colored microbial mats colonise
New Lorne bore runoff channel at different temperatures
5 Samples (4 mats + source sample)
HMW DNA extracted
16S rRNA genes cloned
400 clones studied to determine phylogroups
Representative of each phylogroup sequenced
Phylogenetic Reconstruction
Dic
tyog
lom
us
The
rmod
esu
lfob
acte
ria
Bacteroides
The
rmom
icro
bia
Aq
uif
ex
Chr
ysio
gene
tes
The
rmot
oga
Th
erm
ales
Firm
icut
esChlamydia
Def
erri
bact
er
Nit
rosp
ira
Cya
noba
cter
iaActinobacteria
Verrucomicrobia
Acidobacteria
PlanctomycetesFibrobacter
Spirochetes FusiformsPro
teob
acte
ria
Microbial diversity of the New Lorne bore & the 4 runoff channel microbial mats (10 / 21 kingdoms)
1 23 4
5
6
7
8
9
10
6Microbial Gene Research & Resources Facility (MGRRF)Institute for Molecular & Cellular Therapies (IMCT)Biomolecular & Biomedical Sciences (BBS)
Grey Red Green Brown(75ºC) (66ºC) (57ºC) (52ºC)
Bacillus þ
Hydrogenobacter þ þ
Cytophagales þ þ
Clostridia þ þ
Thermus þ þ þ
Meiothermus þ þ þ
Planctomycetes þ þ þ
Chloroflexus þ þ þ
Cyanobacteria þ þ
Acidobacteri þ þa-Proteobacteria þ þ
b-Proteobacteria þ þ
g-Proteobacteria þ þ
d-Proteobacteria þNitrospira þSporomusa þ
Environental Clones þ
Prosthecobacter þ
Group-genus-species distribution
The microbial diversity profile of the 4 microbial mats
Phylum Genus / species Source (89 ºC)
Grey(75 ºC)
Red(66 ºC)
Green(57 ºC)
Brown(52 ºC)
AquificaeH. subterranea 100 6 3
DeinococciThermus T. scotoductus 62 4
T. igniterrae 12 3T. oshimai 1
Meiothermus M. ruber 47 4M. cerberus 2 34
Chloroflexi C. auranticus 4 10 3Nitrospira N. muscoviensis 4Verrucomicrobia 1Cyanobacteria F. musicola 36 1Planctomycetes 1 2 5Acidobacteria 2 8Bacteroidetes 3 6
Proteobacteria
a- Proteobacteria 1 20
b- Proteobacteria 13 2
d- Proteobacteria 2
Firmicutes 8 8 1
Novel,Unaffiliated??
9 24 21 21
Quantitative diversity based on clones (in %)