- OPPF-UK: Home throughput crystallization. The OPPF-UK is open to all UK academic groups and is...
2
Your Neighborhood Specialist For more information please visit: www.oppf.ox.ac.uk The OPPF-UK is run through the Nuffield Department of Clinical Medicine, University of Oxford and is funded by: The OPPF-UK is core facility for protein production located in a multi- disciplinary Research Complex (RCaH) at the Rutherford Appleton Laboratory. Access We offer: High throughput vector construction and expression screening. Protein production in insect and mammalian cells. High throughput crystallization. The OPPF-UK is open to all UK academic groups and is FREE to users. The Oxford Protein Production Facility UK Research Complex at Harwell R92 Rutherford Appleton Laboratory, Harwell Oxford Oxfordshire OX11 0FA. Tel: (+44) (0)1235 567727 Email: [email protected] Photographs: © STFC, © MRC, © OPPF PPF-UK
Transcript of - OPPF-UK: Home throughput crystallization. The OPPF-UK is open to all UK academic groups and is...
Your Neighborhood Specialist
For more information please visit: www.oppf.ox.ac.uk
The OPPF-UK is run through
the Nuff ie ld Department of
Cl inical Medicine, Universi ty
of Oxford and is funded by:
The OPPF-UK is core facility for protein production located in a multi-disciplinary Research Complex (RCaH) at the Rutherford Appleton Laboratory.
Access We offer: High throughput vector construction and expression screening. Protein production in insect and mammalian cells. High throughput crystallization. The OPPF-UK is open to all UK academic groups and is FREE to users.
The Oxford Protein Production Facility UK Research Complex at Harwell R92 Rutherford Appleton Laboratory, Harwell Oxford Oxfordshire OX11 0FA.
Tel: (+44) (0)1235 567727
Email: [email protected]
Photographs: © STFC, © MRC, © OPPF
PPF-UK
The Oxford Protein Production Facility-UK A NATI ONAL RES OURCE CE NTRE FOR P ROTEI N S CI E NCE
The OPPF-UK was established in 2010 to provide protein production and crystallization
services to support UK Life Sciences.
MAKING AN APPLICATION
REGISTER AT WWW.OPPF.OX.AC.UK
SELECT NEW PROPOSAL TAB.
COMPLETE & SUBMIT FORM.
PROPOSAL REVIEWED BY ACCESS PANEL.
RESPONSE WITHIN TWO WEEKS.
OPPF VECTORS
HIGH THROUGHPUT CLONING USING IN-FUSION™.
EXPRESSION IN MULTIPLE CELL HOSTS.
HEXA-HISTIDINE TAGGED (N OR C-TERMINUS).
SECRETED OR INTRACELLULAR.
CHOICE OF FUSIONS, E.G. GST, TRX, MBP.
VECTORS FREELY AVAILABLE.
DESIGN
ADVICE ON SELECTION OF VECTORS.
DESIGN OF UP TO 48 CONSTRUCTS/PROTEINS.
DESIGN & SUPPLY OF PCR PRIMERS.
PRODUCTION
CLONING & EXPRESSION SCREENING.
SUPPLY OF VECTORS & RESULTS.
SCALE-UP & PURIFICATION.
QUALITY ASSESSMENT BY MASS SPECTROMETRY
CRYSTALLIZATION
SCREENING & OPTIMIZATION.
AUTOMATED CRYSTAL IMAGING.
REMOTE WEB-BASED VIEWING OF IMAGES.
We h a ve d e ve l o p e d a ra n g e o f h i g h l y sp e c i a l i ze d te ch n o l og i e s i n co rp o ra t i n g ro b o t i c sys te ms to e n a b l e th e h i g h
th rou g h p u t e xpre ss i on , p u r i f i ca t i o n an d c r ys ta l l i za t io n o f r e co mb i n a n t p ro te i n s .
Proteins produced in the OPPF-UK have been used for the production of antibodies, assay development and structure solution.
