磁性粒子及檢測的介紹. 如果是磁鐵人,磁場可能是如何產生 ? The real-life...
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Transcript of 磁性粒子及檢測的介紹. 如果是磁鐵人,磁場可能是如何產生 ? The real-life...
磁性粒子及檢測的介紹
2
http://www.2spare.com/item_91569.aspx如果是磁鐵人,磁場可能是如何產生 ?
The real-life Magnetic Man Published on 11/16/2007
Magnetic field sensors
Magnetometers Gaussmeters
SQUID Flux gateMagnetoresistance
Hall effect MagnetoresistanceMagnetodiodeMagnetotransistor
H > 1 mTH < 1 mT
10-15 10-12 10-9 10-6
T
LTS SQUID
HTS SQUID
Flux gate (300 K)
magnetoresistance
Earth field Brain
Heart
Magnetic immunoassay
磁性儀器量測範圍
Fig. 1. SQUID magnetometer principle Commercial SQUID magnetometer: the most sensitive available instrument for measuring in a research laboratory the magnetic moment of a sample.
商業化的 SQUID 量測儀器,其可提供加到到 7 T 的磁場,可量測磁滯曲線,磁性物質等等
Biosensor• Detector • Transducer• Sensitive biological sensor
傳統免疫檢測 Immunosorbent Assay (ELISA)
Serum with
Test chip
On-chip bio-probe (anti-gen) Adding
indicators
Fluorescence/Isotopes Wash/separation
The amount of is probed by detecting the intensities of fluorescence/isotopes
以測抗體而言
動機
磁標記方式
Magnetic particle
Bio-probe
Bio-target
Magnetic properties of clusters are measured to detect the amount of bio-target (antibody).
Origa, R. et al. Haematologica 2007;92, 583-588
Iron distribution in the liver of thalassemia intermedia (A) and major (B) patients
Transport of a magnetic marker through esophagus, stomach, and duodenum( 十二指腸 ) in a frontal projection. The dots represent marker localizations in time steps of 40 ms; altogether 40 minutes of the transport are displayed.
Magnetic properties of the magnetic clusters detected in MLI:
Magnetic Relaxation1,2 ( 磁鬆弛免疫量測 ) Mixed Frequency ac Magnetic Susceptibility3
( 混頻交流磁導率免疫量測 ) Magnetic Remanence4( 磁殘免疫量測 ) Saturated Magnetization ( 飽和磁化 )
1R. Kütitz et al., JMMM, 194, 62(1999) 2J. Clarke et al., APL, 81, 3094(2002) 3Y. Zhang et al., Deutsche Patentanmeldung 10309132.7(2003) 4K. Enpuku et al., JJAP, 38, L1102(1999)
Nano-Magnetic particles
• Fabrication • Size• Stability• Drug delivery
磁性流體製程圖
Fe3O4 Dextran
Water
H.E. Horng et al., J. Magn. Magn. Mater., 283, 210 (2004)
• 葡聚醣披覆之 Fe3O4 磁珠
鏈球菌披覆之 Fe3O4 磁珠 生物探針披覆之 Fe3O4 磁珠
磁性粒子量測• 磁減量檢測( ImmunoMagnetic Reduction, IMR ) 在外加交流磁場頻率,當頻率在幾十到幾百萬赫茲時,磁
性試劑中的單顆磁珠會受到外加交流磁場的驅動,產生旋轉,磁性試劑因而產生交流磁訊號 (ac) 。
樣品透過磁珠表面上的抗體,便可與磁性粒子相結合。如此,試劑中的部份磁珠粒子將會變大,造成數顆磁珠聚集在一起。在此情況下,磁性試劑中可受外加磁場驅動而旋轉的磁珠數目,與先前未與待測樣品混合時的旋轉磁珠數目相較,會少了許多。磁性試劑的交流磁訊號 (ac) 會因待測樣品中受檢生物分子與磁珠的結合,而被降低,此即我們稱此檢測方式為磁減量檢測的原因。並且,由上述中可知,當樣品中含有越多的待測生物分子時,將有越多的磁珠與待測生物分子相結合,因此將會表現出更大的磁減量。所以,透過磁性試劑的磁減量量測,我們可測得樣品中待測生物分子的含量