Β-glucan in wound healing Ri 謝博帆 Ri 謝博帆. Wound healing

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Transcript of Β-glucan in wound healing Ri 謝博帆 Ri 謝博帆. Wound healing

  • -glucan in wound healing

    Ri

  • Wound healing

  • -glucana fiber-type complex sugar (polysaccharide) derived from the cell wall of plants, fungi, and certain bacteria

  • Physiology of -glucanImmunomodulator: - activation of macrophage - phygocytosis of neutrophil - NK cell, complement system - activation of lymphocyteLowering cholesterol levelModifying neoplastic disease

  • Effect of -glucan on wound healing Activation of fibroblast - transcription factor (AP-1, Sp1, NF-B, NF-1) production of PDGF-A&B, FGF-a&b, TGF-&, VEGF, NT-3, etc. macrophage infiltration, reepithelialization, collagen deposition, increase tensile strengthStimulation of macrophage

  • Applications of -glucanI. Effectiveness of beta-glucan collagen for treatment of partial-thickness burns in children

    II. Bio-artificial skin composed of gelatin and (13), (16)--glucan

  • I. -glucan collagen for treatment of partial-thickness burns of children

    BGC = beta-glucan collagen matrix

    BGC as a temporary wound dressing, intended for management of partial-thickness burns, donor sites, and shallow wounds

  • Discussion Outcome of burn injuries was not affected adversely by the choice of BGC or standard treatment with silver sulfadiazineAdvantage of BGC: - lack of daily wound care (painful dressing) - provide a semiocclusive wound covering, decrease evaporative water and heat loss - effective barrier to bacterial contamination - allow improved physical and occupational therapy - lower financial expense

  • II. Bio-artificial skin composed of gelatin and (13), (16)--glucan Dermal substitute: function as a guide for cells moving into the repair area, serve as a scaffold, and help synthesize extracellular matrix (ECM)Porous gelatin/-glucan sponge containing fibroblast (gelatin: denatured type of collagen, activating macrophages, and having hemostatic effect)

  • Material and methodsSponge cultured with fibroblast: artificial dermisDermal equivalent cultured with keratinocyte: artificial epidermisAnimal test: - full-thickness wound, 1cm in diameter, on the back of mice - H&E stain of the wound tissue after 1 week

  • Result (1)Cellular attachment and proliferationGel 100 & Gg91

  • Result (2) Morphology of scaffoldsGel 100Gg 91Gg 73Gg 55

  • Result (3)Distribution of fibroblast for 2 weeks

  • Result (4)Massons trichrome staining for extracellular matrix (ECM) of Gg91 with fibroblasts

  • Result (5)Keratinocytes and fibroblasts cultured in Gg91 sponge

  • Result (6)H&E stain of mouse skin woundGel 100Gg 55Gel 100 without fibroblasts

  • Discussion (1) Cell attachment and proliferation depend on the contact angle, the charge on the material surface, functional groups, and the surface texture - Moderate contact angle: controlled by mixing the appropriate ratios of gelatin and-glucan - Positive charge of acidic residues of gelatin - Arginine-glycine-aspartic acid (RGD) group of gelatin: binding with integrin receptor, promoting adhesion, regulating cell growth, differentiation

    Gel 100 & Gg91 are good for cell attachment and proliferation

  • Discussion (2)-glucan: - modification of hydrophilic state - modification of pore morphology - bioacitve wound healingGelatin: - promote wound healing - form a dense surface (protection from evaporation from the wound, while having a detrimental effect on cell migration) low concentration (0.7 wt%)

  • Discussion (3)Fibroblasts in dermal equivalent: - stimulate epidermal differentiation - release cytokines, accelerating the healing process - enhance the attachment of keratinocyte improve the re-epithelialization on the full-thickness defect

  • Conclusion -glucan promotes wound healing by activation of macrophages and fibroblastsBio-artificial skin containg -glucan

  • ReferenceKougias P, Wei D, Rice PJ, Ensley HE, Kalbfleisch J, Willams DL, Browder IW. Normal human fibroblasts express pattern recognition receptors for fungal (13)--D-glucans. Infect Immunity 69:3933-3938, 2001Delatte SJ, Evans J, Hebra A, Adamson W, Othersen HB, Tagge EP. Effectiveness of beta-glucan collagen for treatment of partial-thickness burns in children. J Pediatr Surg 36:113-118, 2001. Wei D, Williams D, Browder W. Activation of AP-1 and SP1 correlates with eound growth factor gene expression in glucan-treated human fibroblasts. Int Immunopharmacology 2:1163-1172, 2002aWei D, Zhang L, Williams D, Browder W. Glucan stimulates human dermal fibroblast collagen biosythesis through a nuclear factor-1 depenent mechanism . Int Immunopharmacology 10:161-168, 2002bLee SB, Jeon HW, Lee YW, Lee YM, Song KW, Park MH, Nam YS, Ahn HC Bio-artifical skin composed of gelatin and (1 3),(16)--glucan . Biomaterials 24:2503-2511, 2003. Pillai, R.;Redmond, M.;Rding, J. Anti-Wrinkle Therapy: Significant New Findings in the Non-Invasive Cosmetic Treatment of Skin Wrinkles with Beta-Glucan. Int. journal of cosmetic science, Volume 27,Number 5, October 2005, pp. 292-292(1)

    Daily dressing, topical silver sulfadiazineMW, viscosity of betaglucanBetaglucan, cell attachmentBlue ECMscaffold