實驗數據分析與圖表製作 Data Anal. and Fig/Table Preps
description
Transcript of 實驗數據分析與圖表製作 Data Anal. and Fig/Table Preps
LC Chen at BIME NTU
實驗數據分析與圖表製作實驗數據分析與圖表製作Data Anal and FigTable Data Anal and FigTable PrepsPreps
Lin-Chi Chen ( 陳林祈 )
Dept of Bio-Industrial Mechatronics EngNational Taiwan University
Email chenlinchintuedutw Tel 02-3366-5343
20082008 生物產業機電工程特論 生物產業機電工程特論 (840 ndash 930 am on Oct 23 2008)(840 ndash 930 am on Oct 23 2008)
LC Chen at BIME NTU
LC Chen at BIME NTU
Structure of a Scientific Paper
Most journals will require the same basic sections
1048708Abstract (摘要)1048708Introduction (導論) Background + Objective(s)
1048708Materials and Methods (研究方法)1048708Results (結果)1048708Discussion (討論)1048708Conclusion(s) (結論)1048708References (Literature Cited) (參考文獻)1048708Tables Figures Figure Legends (圖表及說明)
LC Chen at BIME NTU
Planning for a Scientific Manuscript
Determine journalrsquos requirements (Instructions to Authors)bull Length of manuscript
bull Number of illustrationsfigures
bull Number of tables
bull Format of final submission
bull Check journalrsquos Web site or in journal itself for these and other specifications
The manuscript should be sequential and logical includingbull Statement of problem (hypothesis)
bull Presentation of the evidence
bull Validity of the evidence
bull Implications of the evidence
bull Assessment of answerrsquos validity
bull Conclusion
Require Figures and Tables in Support of the ldquoFindingsrdquo
LC Chen at BIME NTU
資料來源蘋果日報
How do nowadays newspapers present the figures and tables in support of the ldquostoriesrdquo determined by the editor
LC Chen at BIME NTU
httpabacusbatesedu~gandersobiologyresourceswritingHTWtablefigshtml
The way to present the figures and tables in support of the ldquofindingsrdquo by scientific researchers is somewhat different
LC Chen at BIME NTU
Three Forms of Data Presentation
Text contrary to what you may have heard not all analyses or results warrant a Table or Figure Some simple results are best stated in a single sentence with data summarized parenthetically
Seed production was higher for plants in the full-sun treatment (523 +-68 seeds) than for those receiving filtered light (147+- 32 seeds t=118 df=55 plt0001)
Tables Tables present or summarize lists of numbers or text in columns each column having a title or label Do not use a table when you wish to show a trend or a pattern of relationship between sets of values - these are better presented in a Figure
Figures Figures are visual presentations of results including graphs diagrams photos drawings schematics maps etc Graphs are the most common type of figure Graphs show trends or patterns of relationship
LC Chen at BIME NTU
How to Refer Tables and Figures
How to refer to Tables and Figures from the text Every Figure and Table included in the paper MUST be referred to from the text Use sentences that draw the readers attention to the relationship or trend you wish to highlight referring to the appropriate Figure or Table only parenthetically
Ex Germination rates were significantly higher after 24 h in running water than in controls (Fig 4)
Ex DNA sequence homologies for the purple gene from the four congeners (Table 1) show high similarity differing by at most 4 base pairs
Avoid sentences that give no information other than directing the reader to the Figure or Table
Ex Table 1 shows the summary results for male and female heights at Bates College
LC Chen at BIME NTU
Numbering Placement amp Foolproof
How to number Tables and Figures Figures and Tables are numbered independently in the sequence in which you refer to them in the text starting with Figure 1 and Table 1
Placement of Figures and Tables within the Paper In consideration of your readers place each Table or Figure as near as possible to the place where you first refer to it (eg the next page) For manuscripts (eg lab papers) Tables and Figures are usually put on separate pages from text material
The Acid Test for Tables and Figures Any Table or Figure you present must be sufficiently clear well-labeled and described by its legend to be understood by your intended audience without reading the results section ie it must be able to stand alone and be interpretable Overly complicated Figures or Tables may be difficult to understand in or out of context so strive for simplicity whenever possible If you are unsure whether your tables or figures meet these criteria give them to a fellow biology major (not in your course) and ask them to interpret your results
LC Chen at BIME NTU
Descriptive Legends and Captions
bull Descriptive Legends or Captions To pass the acid test above a clear and complete legend (sometimes called a caption) is essential Like the title of the paper itself each legend should convey as much information as possible about what the Table or Figure Do not simply restate the axis labels with a versus written in between
bull Example Figure 1 Height frequency () of White Pines (Pinus strobus) at Thorncrag Bird Sanctuary Lewiston Maine before and after the Ice Storm of 98 Before n=137 after n=133 Approximately 16 of canopy pines were topped by heavy ice loads averaging 36 m stem length decrease Four trees fell during the storm and were excluded from the post-storm survey
Where do you place the legend
bull Table legends go above the body of the Table and are left justified Tables are read from the top down
bull Figure legends go below the graph graphs and other types of Figures are usually read from the bottom up
LC Chen at BIME NTU
Table Prep Rule of Thumb
bull Determine journalrsquos limitations on number style or structure of tables
bull Tables should be useful (not too simple) and easy to understand (not too complex)
bull Use tables when exact values are important
bull Use tables when they are more efficient to summarize results than in long complex sentences
bull Tables with footnotes and abbreviations should be easily understandable
bull General rule 1 table per 1000 words of text
LC Chen at BIME NTU
Anatomy of Table
The typical layout of a table in three sections demarcated by lines
LC Chen at BIME NTU
Tables are most easily constructed
using your word processors table
function or a spread sheet such as
Excel Gridlines or boxes commonly
invoked by word processors are
optional for our purposes but
unlikely to be permitted in a journal
LC Chen at BIME NTU
Example Literature Comparison
LC Chen at BIME NTU
units are specified in column headings wherever appropriate
Example Summary of Readings
LC Chen at BIME NTU
Types of illustrations
bull Chartsgraphs (bar graph frequency histogram XY scatter plot XY line graph)
bull Photographs (including X-rays SEM DNAprotein image)
bull Line drawings (schematic illustration instrumentation)
bull Use to provide evidence emphasis or efficiency
bull As with tables data in charts may be easier to understand than long statements of text
bull Figures with legends and abbreviations should be easily understandable
Figure Prep Rule of Thumb
LC Chen at BIME NTU
bull Determine journalrsquos limitations on number size and types of figuresillustrations
bull Black and white vs colorbull Journal may not accept one or the other
bull Journal may charge for use of color
bull Resolution of images typical requirementsbull At least 300 dpi for photographs
bull 1200 dpi for line art
bull May require glossy prints (hard copies) or specific electronic file types
bull Label all figures according to journalrsquos specifications
bull Clearly mark the correct orientation of the photograph
bull Provide figure legends describing what is shown
Figure Preparation (Contrsquod)
LC Chen at BIME NTU
Anatomy of Figures (Graph)
LC Chen at BIME NTU
Anatomy of Figures (Chart)
LC Chen at BIME NTU
Graph Example Bar Chart
LC Chen at BIME NTU
Graph Example Frequency Histogram
LC Chen at BIME NTU
Graph Example X-Y Scatter Plot
LC Chen at BIME NTU
Graph Example X-Y Line Plot
LC Chen at BIME NTU
ScatterLine Plot Curve Fitting
LC Chen at BIME NTU
Photograph Example SEM
LC Chen at BIME NTU
Photograph Example DC Picture
(Before charging) (Charging by PV)
(Fully charged) (Removal of PV)
LC Chen at BIME NTU
0-35 AS
35-70 AS
70-100 AS
Done by TzengFEB 2005
1o Ammonium Sulfate Precipitation
2o Anion Exchange Chromatography
Photograph Example Protein PAGE
LC Chen at BIME NTU
Daytime application an IWECB-
based window can be charged and
colored by a photovoltaic cell
(1) to store solar energy and
(2) to attenuate solar irradiation
Nighttime application an IWECB-
based window can be discharged amp
bleached
(1) to drive low-power devices and
(2) to increase the illumination
Line Drawing Schematic Illustration
LC Chen at BIME NTU
Line Drawing Method and Theory
LC Chen at BIME NTU
Line Drawing Experimental Setup
LC Chen at BIME NTU
01 1 10 100 1000
10x106
10x107
S = SO + S
MAX [A] (K
D + [A]) + k[A]
Ideal model for fitting total bindingK
D = 8 nM k = 7944 K
N ~ O (1 M)
Typ
hoo
n R
eadin
gAptamer No108 Conc (nM)
Surface for TP immobilization
Protein
Aptamer
Biotin
Streptavidin (SA)
Alkaline Phosphatase (AP) Unreacted
Substrate
ReactedSubstrate
Enzyme-Linked Immunosorbent Assay (ELISA)
SA-Biotin interaction Kd ~ 10-15 M = fM ~ ca 260 pNt
Typhoon scan aptamer 5-fold dilution series
Aptamer with nM affinity confirmed
Kd = 8 nM
Combination of Figure Presentation
LC Chen at BIME NTU
Frequently Used Software
bull Word
bull PowerPoint
bull CorelDraw
bull Photoshop
bull Excel
bull Origin
bull Grapher
bull SigmaPlot
bull Othershellip
LC Chen at BIME NTU
LC Chen at BIME NTU
A final and essential step for data presentation consider the efficiency (do things right) and effectiveness (do the right things) Recall the model of ldquoApple Dailyrdquo
If you canrsquot read more papershellip
Can you tell the difference between the figures in a rejected paper and an accepted paper
LC Chen at BIME NTU
同學在論文圖表製作過程中常見違反學術倫理而不自覺的情事
直接剪貼網路( Internet )或文獻( PDF )圖片但未經授權甚至未合理引用 ( cf 原文引用)
製作漂亮數據或使用合成照片以滿足自己的結論
未重複實驗僅報導成功一次的數據
LC Chen at BIME NTU
Essence of Scientific ResearchA valuable thesis is based on the REPRODUCIBLE FACTS AND FIGURES and the most importantly YOUR OWN CREACTIONS NOT NUMBER OF PAGES
httpwwwcartoonstockcomdirectorysscientistasp
LC Chen at BIME NTU
LC Chen at BIME NTU
Structure of a Scientific Paper
Most journals will require the same basic sections
1048708Abstract (摘要)1048708Introduction (導論) Background + Objective(s)
1048708Materials and Methods (研究方法)1048708Results (結果)1048708Discussion (討論)1048708Conclusion(s) (結論)1048708References (Literature Cited) (參考文獻)1048708Tables Figures Figure Legends (圖表及說明)
LC Chen at BIME NTU
Planning for a Scientific Manuscript
Determine journalrsquos requirements (Instructions to Authors)bull Length of manuscript
bull Number of illustrationsfigures
bull Number of tables
bull Format of final submission
bull Check journalrsquos Web site or in journal itself for these and other specifications
The manuscript should be sequential and logical includingbull Statement of problem (hypothesis)
bull Presentation of the evidence
bull Validity of the evidence
bull Implications of the evidence
bull Assessment of answerrsquos validity
bull Conclusion
Require Figures and Tables in Support of the ldquoFindingsrdquo
LC Chen at BIME NTU
資料來源蘋果日報
How do nowadays newspapers present the figures and tables in support of the ldquostoriesrdquo determined by the editor
LC Chen at BIME NTU
httpabacusbatesedu~gandersobiologyresourceswritingHTWtablefigshtml
The way to present the figures and tables in support of the ldquofindingsrdquo by scientific researchers is somewhat different
LC Chen at BIME NTU
Three Forms of Data Presentation
Text contrary to what you may have heard not all analyses or results warrant a Table or Figure Some simple results are best stated in a single sentence with data summarized parenthetically
Seed production was higher for plants in the full-sun treatment (523 +-68 seeds) than for those receiving filtered light (147+- 32 seeds t=118 df=55 plt0001)
Tables Tables present or summarize lists of numbers or text in columns each column having a title or label Do not use a table when you wish to show a trend or a pattern of relationship between sets of values - these are better presented in a Figure
Figures Figures are visual presentations of results including graphs diagrams photos drawings schematics maps etc Graphs are the most common type of figure Graphs show trends or patterns of relationship
LC Chen at BIME NTU
How to Refer Tables and Figures
How to refer to Tables and Figures from the text Every Figure and Table included in the paper MUST be referred to from the text Use sentences that draw the readers attention to the relationship or trend you wish to highlight referring to the appropriate Figure or Table only parenthetically
Ex Germination rates were significantly higher after 24 h in running water than in controls (Fig 4)
Ex DNA sequence homologies for the purple gene from the four congeners (Table 1) show high similarity differing by at most 4 base pairs
Avoid sentences that give no information other than directing the reader to the Figure or Table
Ex Table 1 shows the summary results for male and female heights at Bates College
LC Chen at BIME NTU
Numbering Placement amp Foolproof
How to number Tables and Figures Figures and Tables are numbered independently in the sequence in which you refer to them in the text starting with Figure 1 and Table 1
Placement of Figures and Tables within the Paper In consideration of your readers place each Table or Figure as near as possible to the place where you first refer to it (eg the next page) For manuscripts (eg lab papers) Tables and Figures are usually put on separate pages from text material
The Acid Test for Tables and Figures Any Table or Figure you present must be sufficiently clear well-labeled and described by its legend to be understood by your intended audience without reading the results section ie it must be able to stand alone and be interpretable Overly complicated Figures or Tables may be difficult to understand in or out of context so strive for simplicity whenever possible If you are unsure whether your tables or figures meet these criteria give them to a fellow biology major (not in your course) and ask them to interpret your results
LC Chen at BIME NTU
Descriptive Legends and Captions
bull Descriptive Legends or Captions To pass the acid test above a clear and complete legend (sometimes called a caption) is essential Like the title of the paper itself each legend should convey as much information as possible about what the Table or Figure Do not simply restate the axis labels with a versus written in between
bull Example Figure 1 Height frequency () of White Pines (Pinus strobus) at Thorncrag Bird Sanctuary Lewiston Maine before and after the Ice Storm of 98 Before n=137 after n=133 Approximately 16 of canopy pines were topped by heavy ice loads averaging 36 m stem length decrease Four trees fell during the storm and were excluded from the post-storm survey
Where do you place the legend
bull Table legends go above the body of the Table and are left justified Tables are read from the top down
bull Figure legends go below the graph graphs and other types of Figures are usually read from the bottom up
LC Chen at BIME NTU
Table Prep Rule of Thumb
bull Determine journalrsquos limitations on number style or structure of tables
bull Tables should be useful (not too simple) and easy to understand (not too complex)
bull Use tables when exact values are important
bull Use tables when they are more efficient to summarize results than in long complex sentences
bull Tables with footnotes and abbreviations should be easily understandable
bull General rule 1 table per 1000 words of text
LC Chen at BIME NTU
Anatomy of Table
The typical layout of a table in three sections demarcated by lines
LC Chen at BIME NTU
Tables are most easily constructed
using your word processors table
function or a spread sheet such as
Excel Gridlines or boxes commonly
invoked by word processors are
optional for our purposes but
unlikely to be permitted in a journal
LC Chen at BIME NTU
Example Literature Comparison
LC Chen at BIME NTU
units are specified in column headings wherever appropriate
Example Summary of Readings
LC Chen at BIME NTU
Types of illustrations
bull Chartsgraphs (bar graph frequency histogram XY scatter plot XY line graph)
bull Photographs (including X-rays SEM DNAprotein image)
bull Line drawings (schematic illustration instrumentation)
bull Use to provide evidence emphasis or efficiency
bull As with tables data in charts may be easier to understand than long statements of text
bull Figures with legends and abbreviations should be easily understandable
Figure Prep Rule of Thumb
LC Chen at BIME NTU
bull Determine journalrsquos limitations on number size and types of figuresillustrations
bull Black and white vs colorbull Journal may not accept one or the other
bull Journal may charge for use of color
bull Resolution of images typical requirementsbull At least 300 dpi for photographs
bull 1200 dpi for line art
bull May require glossy prints (hard copies) or specific electronic file types
bull Label all figures according to journalrsquos specifications
bull Clearly mark the correct orientation of the photograph
bull Provide figure legends describing what is shown
Figure Preparation (Contrsquod)
LC Chen at BIME NTU
Anatomy of Figures (Graph)
LC Chen at BIME NTU
Anatomy of Figures (Chart)
LC Chen at BIME NTU
Graph Example Bar Chart
LC Chen at BIME NTU
Graph Example Frequency Histogram
LC Chen at BIME NTU
Graph Example X-Y Scatter Plot
LC Chen at BIME NTU
Graph Example X-Y Line Plot
LC Chen at BIME NTU
ScatterLine Plot Curve Fitting
LC Chen at BIME NTU
Photograph Example SEM
LC Chen at BIME NTU
Photograph Example DC Picture
(Before charging) (Charging by PV)
(Fully charged) (Removal of PV)
LC Chen at BIME NTU
0-35 AS
35-70 AS
70-100 AS
Done by TzengFEB 2005
1o Ammonium Sulfate Precipitation
2o Anion Exchange Chromatography
Photograph Example Protein PAGE
LC Chen at BIME NTU
Daytime application an IWECB-
based window can be charged and
colored by a photovoltaic cell
(1) to store solar energy and
(2) to attenuate solar irradiation
Nighttime application an IWECB-
based window can be discharged amp
bleached
(1) to drive low-power devices and
(2) to increase the illumination
Line Drawing Schematic Illustration
LC Chen at BIME NTU
Line Drawing Method and Theory
LC Chen at BIME NTU
Line Drawing Experimental Setup
LC Chen at BIME NTU
01 1 10 100 1000
10x106
10x107
S = SO + S
MAX [A] (K
D + [A]) + k[A]
Ideal model for fitting total bindingK
D = 8 nM k = 7944 K
N ~ O (1 M)
Typ
hoo
n R
eadin
gAptamer No108 Conc (nM)
Surface for TP immobilization
Protein
Aptamer
Biotin
Streptavidin (SA)
Alkaline Phosphatase (AP) Unreacted
Substrate
ReactedSubstrate
Enzyme-Linked Immunosorbent Assay (ELISA)
SA-Biotin interaction Kd ~ 10-15 M = fM ~ ca 260 pNt
Typhoon scan aptamer 5-fold dilution series
Aptamer with nM affinity confirmed
Kd = 8 nM
Combination of Figure Presentation
LC Chen at BIME NTU
Frequently Used Software
bull Word
bull PowerPoint
bull CorelDraw
bull Photoshop
bull Excel
bull Origin
bull Grapher
bull SigmaPlot
bull Othershellip
LC Chen at BIME NTU
LC Chen at BIME NTU
A final and essential step for data presentation consider the efficiency (do things right) and effectiveness (do the right things) Recall the model of ldquoApple Dailyrdquo
If you canrsquot read more papershellip
Can you tell the difference between the figures in a rejected paper and an accepted paper
LC Chen at BIME NTU
同學在論文圖表製作過程中常見違反學術倫理而不自覺的情事
直接剪貼網路( Internet )或文獻( PDF )圖片但未經授權甚至未合理引用 ( cf 原文引用)
製作漂亮數據或使用合成照片以滿足自己的結論
未重複實驗僅報導成功一次的數據
LC Chen at BIME NTU
Essence of Scientific ResearchA valuable thesis is based on the REPRODUCIBLE FACTS AND FIGURES and the most importantly YOUR OWN CREACTIONS NOT NUMBER OF PAGES
httpwwwcartoonstockcomdirectorysscientistasp
LC Chen at BIME NTU
Structure of a Scientific Paper
Most journals will require the same basic sections
1048708Abstract (摘要)1048708Introduction (導論) Background + Objective(s)
1048708Materials and Methods (研究方法)1048708Results (結果)1048708Discussion (討論)1048708Conclusion(s) (結論)1048708References (Literature Cited) (參考文獻)1048708Tables Figures Figure Legends (圖表及說明)
LC Chen at BIME NTU
Planning for a Scientific Manuscript
Determine journalrsquos requirements (Instructions to Authors)bull Length of manuscript
bull Number of illustrationsfigures
bull Number of tables
bull Format of final submission
bull Check journalrsquos Web site or in journal itself for these and other specifications
The manuscript should be sequential and logical includingbull Statement of problem (hypothesis)
bull Presentation of the evidence
bull Validity of the evidence
bull Implications of the evidence
bull Assessment of answerrsquos validity
bull Conclusion
Require Figures and Tables in Support of the ldquoFindingsrdquo
LC Chen at BIME NTU
資料來源蘋果日報
How do nowadays newspapers present the figures and tables in support of the ldquostoriesrdquo determined by the editor
LC Chen at BIME NTU
httpabacusbatesedu~gandersobiologyresourceswritingHTWtablefigshtml
The way to present the figures and tables in support of the ldquofindingsrdquo by scientific researchers is somewhat different
LC Chen at BIME NTU
Three Forms of Data Presentation
Text contrary to what you may have heard not all analyses or results warrant a Table or Figure Some simple results are best stated in a single sentence with data summarized parenthetically
Seed production was higher for plants in the full-sun treatment (523 +-68 seeds) than for those receiving filtered light (147+- 32 seeds t=118 df=55 plt0001)
Tables Tables present or summarize lists of numbers or text in columns each column having a title or label Do not use a table when you wish to show a trend or a pattern of relationship between sets of values - these are better presented in a Figure
Figures Figures are visual presentations of results including graphs diagrams photos drawings schematics maps etc Graphs are the most common type of figure Graphs show trends or patterns of relationship
LC Chen at BIME NTU
How to Refer Tables and Figures
How to refer to Tables and Figures from the text Every Figure and Table included in the paper MUST be referred to from the text Use sentences that draw the readers attention to the relationship or trend you wish to highlight referring to the appropriate Figure or Table only parenthetically
Ex Germination rates were significantly higher after 24 h in running water than in controls (Fig 4)
Ex DNA sequence homologies for the purple gene from the four congeners (Table 1) show high similarity differing by at most 4 base pairs
Avoid sentences that give no information other than directing the reader to the Figure or Table
Ex Table 1 shows the summary results for male and female heights at Bates College
LC Chen at BIME NTU
Numbering Placement amp Foolproof
How to number Tables and Figures Figures and Tables are numbered independently in the sequence in which you refer to them in the text starting with Figure 1 and Table 1
Placement of Figures and Tables within the Paper In consideration of your readers place each Table or Figure as near as possible to the place where you first refer to it (eg the next page) For manuscripts (eg lab papers) Tables and Figures are usually put on separate pages from text material
The Acid Test for Tables and Figures Any Table or Figure you present must be sufficiently clear well-labeled and described by its legend to be understood by your intended audience without reading the results section ie it must be able to stand alone and be interpretable Overly complicated Figures or Tables may be difficult to understand in or out of context so strive for simplicity whenever possible If you are unsure whether your tables or figures meet these criteria give them to a fellow biology major (not in your course) and ask them to interpret your results
LC Chen at BIME NTU
Descriptive Legends and Captions
bull Descriptive Legends or Captions To pass the acid test above a clear and complete legend (sometimes called a caption) is essential Like the title of the paper itself each legend should convey as much information as possible about what the Table or Figure Do not simply restate the axis labels with a versus written in between
bull Example Figure 1 Height frequency () of White Pines (Pinus strobus) at Thorncrag Bird Sanctuary Lewiston Maine before and after the Ice Storm of 98 Before n=137 after n=133 Approximately 16 of canopy pines were topped by heavy ice loads averaging 36 m stem length decrease Four trees fell during the storm and were excluded from the post-storm survey
Where do you place the legend
bull Table legends go above the body of the Table and are left justified Tables are read from the top down
bull Figure legends go below the graph graphs and other types of Figures are usually read from the bottom up
LC Chen at BIME NTU
Table Prep Rule of Thumb
bull Determine journalrsquos limitations on number style or structure of tables
bull Tables should be useful (not too simple) and easy to understand (not too complex)
bull Use tables when exact values are important
bull Use tables when they are more efficient to summarize results than in long complex sentences
bull Tables with footnotes and abbreviations should be easily understandable
bull General rule 1 table per 1000 words of text
LC Chen at BIME NTU
Anatomy of Table
The typical layout of a table in three sections demarcated by lines
LC Chen at BIME NTU
Tables are most easily constructed
using your word processors table
function or a spread sheet such as
Excel Gridlines or boxes commonly
invoked by word processors are
optional for our purposes but
unlikely to be permitted in a journal
LC Chen at BIME NTU
Example Literature Comparison
LC Chen at BIME NTU
units are specified in column headings wherever appropriate
Example Summary of Readings
LC Chen at BIME NTU
Types of illustrations
bull Chartsgraphs (bar graph frequency histogram XY scatter plot XY line graph)
bull Photographs (including X-rays SEM DNAprotein image)
bull Line drawings (schematic illustration instrumentation)
bull Use to provide evidence emphasis or efficiency
bull As with tables data in charts may be easier to understand than long statements of text
bull Figures with legends and abbreviations should be easily understandable
Figure Prep Rule of Thumb
LC Chen at BIME NTU
bull Determine journalrsquos limitations on number size and types of figuresillustrations
bull Black and white vs colorbull Journal may not accept one or the other
bull Journal may charge for use of color
bull Resolution of images typical requirementsbull At least 300 dpi for photographs
bull 1200 dpi for line art
bull May require glossy prints (hard copies) or specific electronic file types
bull Label all figures according to journalrsquos specifications
bull Clearly mark the correct orientation of the photograph
bull Provide figure legends describing what is shown
Figure Preparation (Contrsquod)
LC Chen at BIME NTU
Anatomy of Figures (Graph)
LC Chen at BIME NTU
Anatomy of Figures (Chart)
LC Chen at BIME NTU
Graph Example Bar Chart
LC Chen at BIME NTU
Graph Example Frequency Histogram
LC Chen at BIME NTU
Graph Example X-Y Scatter Plot
LC Chen at BIME NTU
Graph Example X-Y Line Plot
LC Chen at BIME NTU
ScatterLine Plot Curve Fitting
LC Chen at BIME NTU
Photograph Example SEM
LC Chen at BIME NTU
Photograph Example DC Picture
(Before charging) (Charging by PV)
(Fully charged) (Removal of PV)
LC Chen at BIME NTU
0-35 AS
35-70 AS
70-100 AS
Done by TzengFEB 2005
1o Ammonium Sulfate Precipitation
2o Anion Exchange Chromatography
Photograph Example Protein PAGE
LC Chen at BIME NTU
Daytime application an IWECB-
based window can be charged and
colored by a photovoltaic cell
(1) to store solar energy and
(2) to attenuate solar irradiation
Nighttime application an IWECB-
based window can be discharged amp
bleached
(1) to drive low-power devices and
(2) to increase the illumination
Line Drawing Schematic Illustration
LC Chen at BIME NTU
Line Drawing Method and Theory
LC Chen at BIME NTU
Line Drawing Experimental Setup
LC Chen at BIME NTU
01 1 10 100 1000
10x106
10x107
S = SO + S
MAX [A] (K
D + [A]) + k[A]
Ideal model for fitting total bindingK
D = 8 nM k = 7944 K
N ~ O (1 M)
Typ
hoo
n R
eadin
gAptamer No108 Conc (nM)
Surface for TP immobilization
Protein
Aptamer
Biotin
Streptavidin (SA)
Alkaline Phosphatase (AP) Unreacted
Substrate
ReactedSubstrate
Enzyme-Linked Immunosorbent Assay (ELISA)
SA-Biotin interaction Kd ~ 10-15 M = fM ~ ca 260 pNt
Typhoon scan aptamer 5-fold dilution series
Aptamer with nM affinity confirmed
Kd = 8 nM
Combination of Figure Presentation
LC Chen at BIME NTU
Frequently Used Software
bull Word
bull PowerPoint
bull CorelDraw
bull Photoshop
bull Excel
bull Origin
bull Grapher
bull SigmaPlot
bull Othershellip
LC Chen at BIME NTU
LC Chen at BIME NTU
A final and essential step for data presentation consider the efficiency (do things right) and effectiveness (do the right things) Recall the model of ldquoApple Dailyrdquo
If you canrsquot read more papershellip
Can you tell the difference between the figures in a rejected paper and an accepted paper
LC Chen at BIME NTU
同學在論文圖表製作過程中常見違反學術倫理而不自覺的情事
直接剪貼網路( Internet )或文獻( PDF )圖片但未經授權甚至未合理引用 ( cf 原文引用)
製作漂亮數據或使用合成照片以滿足自己的結論
未重複實驗僅報導成功一次的數據
LC Chen at BIME NTU
Essence of Scientific ResearchA valuable thesis is based on the REPRODUCIBLE FACTS AND FIGURES and the most importantly YOUR OWN CREACTIONS NOT NUMBER OF PAGES
httpwwwcartoonstockcomdirectorysscientistasp
LC Chen at BIME NTU
Planning for a Scientific Manuscript
Determine journalrsquos requirements (Instructions to Authors)bull Length of manuscript
bull Number of illustrationsfigures
bull Number of tables
bull Format of final submission
bull Check journalrsquos Web site or in journal itself for these and other specifications
The manuscript should be sequential and logical includingbull Statement of problem (hypothesis)
bull Presentation of the evidence
bull Validity of the evidence
bull Implications of the evidence
bull Assessment of answerrsquos validity
bull Conclusion
Require Figures and Tables in Support of the ldquoFindingsrdquo
LC Chen at BIME NTU
資料來源蘋果日報
How do nowadays newspapers present the figures and tables in support of the ldquostoriesrdquo determined by the editor
LC Chen at BIME NTU
httpabacusbatesedu~gandersobiologyresourceswritingHTWtablefigshtml
The way to present the figures and tables in support of the ldquofindingsrdquo by scientific researchers is somewhat different
LC Chen at BIME NTU
Three Forms of Data Presentation
Text contrary to what you may have heard not all analyses or results warrant a Table or Figure Some simple results are best stated in a single sentence with data summarized parenthetically
Seed production was higher for plants in the full-sun treatment (523 +-68 seeds) than for those receiving filtered light (147+- 32 seeds t=118 df=55 plt0001)
Tables Tables present or summarize lists of numbers or text in columns each column having a title or label Do not use a table when you wish to show a trend or a pattern of relationship between sets of values - these are better presented in a Figure
Figures Figures are visual presentations of results including graphs diagrams photos drawings schematics maps etc Graphs are the most common type of figure Graphs show trends or patterns of relationship
LC Chen at BIME NTU
How to Refer Tables and Figures
How to refer to Tables and Figures from the text Every Figure and Table included in the paper MUST be referred to from the text Use sentences that draw the readers attention to the relationship or trend you wish to highlight referring to the appropriate Figure or Table only parenthetically
Ex Germination rates were significantly higher after 24 h in running water than in controls (Fig 4)
Ex DNA sequence homologies for the purple gene from the four congeners (Table 1) show high similarity differing by at most 4 base pairs
Avoid sentences that give no information other than directing the reader to the Figure or Table
Ex Table 1 shows the summary results for male and female heights at Bates College
LC Chen at BIME NTU
Numbering Placement amp Foolproof
How to number Tables and Figures Figures and Tables are numbered independently in the sequence in which you refer to them in the text starting with Figure 1 and Table 1
Placement of Figures and Tables within the Paper In consideration of your readers place each Table or Figure as near as possible to the place where you first refer to it (eg the next page) For manuscripts (eg lab papers) Tables and Figures are usually put on separate pages from text material
The Acid Test for Tables and Figures Any Table or Figure you present must be sufficiently clear well-labeled and described by its legend to be understood by your intended audience without reading the results section ie it must be able to stand alone and be interpretable Overly complicated Figures or Tables may be difficult to understand in or out of context so strive for simplicity whenever possible If you are unsure whether your tables or figures meet these criteria give them to a fellow biology major (not in your course) and ask them to interpret your results
LC Chen at BIME NTU
Descriptive Legends and Captions
bull Descriptive Legends or Captions To pass the acid test above a clear and complete legend (sometimes called a caption) is essential Like the title of the paper itself each legend should convey as much information as possible about what the Table or Figure Do not simply restate the axis labels with a versus written in between
bull Example Figure 1 Height frequency () of White Pines (Pinus strobus) at Thorncrag Bird Sanctuary Lewiston Maine before and after the Ice Storm of 98 Before n=137 after n=133 Approximately 16 of canopy pines were topped by heavy ice loads averaging 36 m stem length decrease Four trees fell during the storm and were excluded from the post-storm survey
Where do you place the legend
bull Table legends go above the body of the Table and are left justified Tables are read from the top down
bull Figure legends go below the graph graphs and other types of Figures are usually read from the bottom up
LC Chen at BIME NTU
Table Prep Rule of Thumb
bull Determine journalrsquos limitations on number style or structure of tables
bull Tables should be useful (not too simple) and easy to understand (not too complex)
bull Use tables when exact values are important
bull Use tables when they are more efficient to summarize results than in long complex sentences
bull Tables with footnotes and abbreviations should be easily understandable
bull General rule 1 table per 1000 words of text
LC Chen at BIME NTU
Anatomy of Table
The typical layout of a table in three sections demarcated by lines
LC Chen at BIME NTU
Tables are most easily constructed
using your word processors table
function or a spread sheet such as
Excel Gridlines or boxes commonly
invoked by word processors are
optional for our purposes but
unlikely to be permitted in a journal
LC Chen at BIME NTU
Example Literature Comparison
LC Chen at BIME NTU
units are specified in column headings wherever appropriate
Example Summary of Readings
LC Chen at BIME NTU
Types of illustrations
bull Chartsgraphs (bar graph frequency histogram XY scatter plot XY line graph)
bull Photographs (including X-rays SEM DNAprotein image)
bull Line drawings (schematic illustration instrumentation)
bull Use to provide evidence emphasis or efficiency
bull As with tables data in charts may be easier to understand than long statements of text
bull Figures with legends and abbreviations should be easily understandable
Figure Prep Rule of Thumb
LC Chen at BIME NTU
bull Determine journalrsquos limitations on number size and types of figuresillustrations
bull Black and white vs colorbull Journal may not accept one or the other
bull Journal may charge for use of color
bull Resolution of images typical requirementsbull At least 300 dpi for photographs
bull 1200 dpi for line art
bull May require glossy prints (hard copies) or specific electronic file types
bull Label all figures according to journalrsquos specifications
bull Clearly mark the correct orientation of the photograph
bull Provide figure legends describing what is shown
Figure Preparation (Contrsquod)
LC Chen at BIME NTU
Anatomy of Figures (Graph)
LC Chen at BIME NTU
Anatomy of Figures (Chart)
LC Chen at BIME NTU
Graph Example Bar Chart
LC Chen at BIME NTU
Graph Example Frequency Histogram
LC Chen at BIME NTU
Graph Example X-Y Scatter Plot
LC Chen at BIME NTU
Graph Example X-Y Line Plot
LC Chen at BIME NTU
ScatterLine Plot Curve Fitting
LC Chen at BIME NTU
Photograph Example SEM
LC Chen at BIME NTU
Photograph Example DC Picture
(Before charging) (Charging by PV)
(Fully charged) (Removal of PV)
LC Chen at BIME NTU
0-35 AS
35-70 AS
70-100 AS
Done by TzengFEB 2005
1o Ammonium Sulfate Precipitation
2o Anion Exchange Chromatography
Photograph Example Protein PAGE
LC Chen at BIME NTU
Daytime application an IWECB-
based window can be charged and
colored by a photovoltaic cell
(1) to store solar energy and
(2) to attenuate solar irradiation
Nighttime application an IWECB-
based window can be discharged amp
bleached
(1) to drive low-power devices and
(2) to increase the illumination
Line Drawing Schematic Illustration
LC Chen at BIME NTU
Line Drawing Method and Theory
LC Chen at BIME NTU
Line Drawing Experimental Setup
LC Chen at BIME NTU
01 1 10 100 1000
10x106
10x107
S = SO + S
MAX [A] (K
D + [A]) + k[A]
Ideal model for fitting total bindingK
D = 8 nM k = 7944 K
N ~ O (1 M)
Typ
hoo
n R
eadin
gAptamer No108 Conc (nM)
Surface for TP immobilization
Protein
Aptamer
Biotin
Streptavidin (SA)
Alkaline Phosphatase (AP) Unreacted
Substrate
ReactedSubstrate
Enzyme-Linked Immunosorbent Assay (ELISA)
SA-Biotin interaction Kd ~ 10-15 M = fM ~ ca 260 pNt
Typhoon scan aptamer 5-fold dilution series
Aptamer with nM affinity confirmed
Kd = 8 nM
Combination of Figure Presentation
LC Chen at BIME NTU
Frequently Used Software
bull Word
bull PowerPoint
bull CorelDraw
bull Photoshop
bull Excel
bull Origin
bull Grapher
bull SigmaPlot
bull Othershellip
LC Chen at BIME NTU
LC Chen at BIME NTU
A final and essential step for data presentation consider the efficiency (do things right) and effectiveness (do the right things) Recall the model of ldquoApple Dailyrdquo
If you canrsquot read more papershellip
Can you tell the difference between the figures in a rejected paper and an accepted paper
LC Chen at BIME NTU
同學在論文圖表製作過程中常見違反學術倫理而不自覺的情事
直接剪貼網路( Internet )或文獻( PDF )圖片但未經授權甚至未合理引用 ( cf 原文引用)
製作漂亮數據或使用合成照片以滿足自己的結論
未重複實驗僅報導成功一次的數據
LC Chen at BIME NTU
Essence of Scientific ResearchA valuable thesis is based on the REPRODUCIBLE FACTS AND FIGURES and the most importantly YOUR OWN CREACTIONS NOT NUMBER OF PAGES
httpwwwcartoonstockcomdirectorysscientistasp
LC Chen at BIME NTU
資料來源蘋果日報
How do nowadays newspapers present the figures and tables in support of the ldquostoriesrdquo determined by the editor
LC Chen at BIME NTU
httpabacusbatesedu~gandersobiologyresourceswritingHTWtablefigshtml
The way to present the figures and tables in support of the ldquofindingsrdquo by scientific researchers is somewhat different
LC Chen at BIME NTU
Three Forms of Data Presentation
Text contrary to what you may have heard not all analyses or results warrant a Table or Figure Some simple results are best stated in a single sentence with data summarized parenthetically
Seed production was higher for plants in the full-sun treatment (523 +-68 seeds) than for those receiving filtered light (147+- 32 seeds t=118 df=55 plt0001)
Tables Tables present or summarize lists of numbers or text in columns each column having a title or label Do not use a table when you wish to show a trend or a pattern of relationship between sets of values - these are better presented in a Figure
Figures Figures are visual presentations of results including graphs diagrams photos drawings schematics maps etc Graphs are the most common type of figure Graphs show trends or patterns of relationship
LC Chen at BIME NTU
How to Refer Tables and Figures
How to refer to Tables and Figures from the text Every Figure and Table included in the paper MUST be referred to from the text Use sentences that draw the readers attention to the relationship or trend you wish to highlight referring to the appropriate Figure or Table only parenthetically
Ex Germination rates were significantly higher after 24 h in running water than in controls (Fig 4)
Ex DNA sequence homologies for the purple gene from the four congeners (Table 1) show high similarity differing by at most 4 base pairs
Avoid sentences that give no information other than directing the reader to the Figure or Table
Ex Table 1 shows the summary results for male and female heights at Bates College
LC Chen at BIME NTU
Numbering Placement amp Foolproof
How to number Tables and Figures Figures and Tables are numbered independently in the sequence in which you refer to them in the text starting with Figure 1 and Table 1
Placement of Figures and Tables within the Paper In consideration of your readers place each Table or Figure as near as possible to the place where you first refer to it (eg the next page) For manuscripts (eg lab papers) Tables and Figures are usually put on separate pages from text material
The Acid Test for Tables and Figures Any Table or Figure you present must be sufficiently clear well-labeled and described by its legend to be understood by your intended audience without reading the results section ie it must be able to stand alone and be interpretable Overly complicated Figures or Tables may be difficult to understand in or out of context so strive for simplicity whenever possible If you are unsure whether your tables or figures meet these criteria give them to a fellow biology major (not in your course) and ask them to interpret your results
LC Chen at BIME NTU
Descriptive Legends and Captions
bull Descriptive Legends or Captions To pass the acid test above a clear and complete legend (sometimes called a caption) is essential Like the title of the paper itself each legend should convey as much information as possible about what the Table or Figure Do not simply restate the axis labels with a versus written in between
bull Example Figure 1 Height frequency () of White Pines (Pinus strobus) at Thorncrag Bird Sanctuary Lewiston Maine before and after the Ice Storm of 98 Before n=137 after n=133 Approximately 16 of canopy pines were topped by heavy ice loads averaging 36 m stem length decrease Four trees fell during the storm and were excluded from the post-storm survey
Where do you place the legend
bull Table legends go above the body of the Table and are left justified Tables are read from the top down
bull Figure legends go below the graph graphs and other types of Figures are usually read from the bottom up
LC Chen at BIME NTU
Table Prep Rule of Thumb
bull Determine journalrsquos limitations on number style or structure of tables
bull Tables should be useful (not too simple) and easy to understand (not too complex)
bull Use tables when exact values are important
bull Use tables when they are more efficient to summarize results than in long complex sentences
bull Tables with footnotes and abbreviations should be easily understandable
bull General rule 1 table per 1000 words of text
LC Chen at BIME NTU
Anatomy of Table
The typical layout of a table in three sections demarcated by lines
LC Chen at BIME NTU
Tables are most easily constructed
using your word processors table
function or a spread sheet such as
Excel Gridlines or boxes commonly
invoked by word processors are
optional for our purposes but
unlikely to be permitted in a journal
LC Chen at BIME NTU
Example Literature Comparison
LC Chen at BIME NTU
units are specified in column headings wherever appropriate
Example Summary of Readings
LC Chen at BIME NTU
Types of illustrations
bull Chartsgraphs (bar graph frequency histogram XY scatter plot XY line graph)
bull Photographs (including X-rays SEM DNAprotein image)
bull Line drawings (schematic illustration instrumentation)
bull Use to provide evidence emphasis or efficiency
bull As with tables data in charts may be easier to understand than long statements of text
bull Figures with legends and abbreviations should be easily understandable
Figure Prep Rule of Thumb
LC Chen at BIME NTU
bull Determine journalrsquos limitations on number size and types of figuresillustrations
bull Black and white vs colorbull Journal may not accept one or the other
bull Journal may charge for use of color
bull Resolution of images typical requirementsbull At least 300 dpi for photographs
bull 1200 dpi for line art
bull May require glossy prints (hard copies) or specific electronic file types
bull Label all figures according to journalrsquos specifications
bull Clearly mark the correct orientation of the photograph
bull Provide figure legends describing what is shown
Figure Preparation (Contrsquod)
LC Chen at BIME NTU
Anatomy of Figures (Graph)
LC Chen at BIME NTU
Anatomy of Figures (Chart)
LC Chen at BIME NTU
Graph Example Bar Chart
LC Chen at BIME NTU
Graph Example Frequency Histogram
LC Chen at BIME NTU
Graph Example X-Y Scatter Plot
LC Chen at BIME NTU
Graph Example X-Y Line Plot
LC Chen at BIME NTU
ScatterLine Plot Curve Fitting
LC Chen at BIME NTU
Photograph Example SEM
LC Chen at BIME NTU
Photograph Example DC Picture
(Before charging) (Charging by PV)
(Fully charged) (Removal of PV)
LC Chen at BIME NTU
0-35 AS
35-70 AS
70-100 AS
Done by TzengFEB 2005
1o Ammonium Sulfate Precipitation
2o Anion Exchange Chromatography
Photograph Example Protein PAGE
LC Chen at BIME NTU
Daytime application an IWECB-
based window can be charged and
colored by a photovoltaic cell
(1) to store solar energy and
(2) to attenuate solar irradiation
Nighttime application an IWECB-
based window can be discharged amp
bleached
(1) to drive low-power devices and
(2) to increase the illumination
Line Drawing Schematic Illustration
LC Chen at BIME NTU
Line Drawing Method and Theory
LC Chen at BIME NTU
Line Drawing Experimental Setup
LC Chen at BIME NTU
01 1 10 100 1000
10x106
10x107
S = SO + S
MAX [A] (K
D + [A]) + k[A]
Ideal model for fitting total bindingK
D = 8 nM k = 7944 K
N ~ O (1 M)
Typ
hoo
n R
eadin
gAptamer No108 Conc (nM)
Surface for TP immobilization
Protein
Aptamer
Biotin
Streptavidin (SA)
Alkaline Phosphatase (AP) Unreacted
Substrate
ReactedSubstrate
Enzyme-Linked Immunosorbent Assay (ELISA)
SA-Biotin interaction Kd ~ 10-15 M = fM ~ ca 260 pNt
Typhoon scan aptamer 5-fold dilution series
Aptamer with nM affinity confirmed
Kd = 8 nM
Combination of Figure Presentation
LC Chen at BIME NTU
Frequently Used Software
bull Word
bull PowerPoint
bull CorelDraw
bull Photoshop
bull Excel
bull Origin
bull Grapher
bull SigmaPlot
bull Othershellip
LC Chen at BIME NTU
LC Chen at BIME NTU
A final and essential step for data presentation consider the efficiency (do things right) and effectiveness (do the right things) Recall the model of ldquoApple Dailyrdquo
If you canrsquot read more papershellip
Can you tell the difference between the figures in a rejected paper and an accepted paper
LC Chen at BIME NTU
同學在論文圖表製作過程中常見違反學術倫理而不自覺的情事
直接剪貼網路( Internet )或文獻( PDF )圖片但未經授權甚至未合理引用 ( cf 原文引用)
製作漂亮數據或使用合成照片以滿足自己的結論
未重複實驗僅報導成功一次的數據
LC Chen at BIME NTU
Essence of Scientific ResearchA valuable thesis is based on the REPRODUCIBLE FACTS AND FIGURES and the most importantly YOUR OWN CREACTIONS NOT NUMBER OF PAGES
httpwwwcartoonstockcomdirectorysscientistasp
LC Chen at BIME NTU
httpabacusbatesedu~gandersobiologyresourceswritingHTWtablefigshtml
The way to present the figures and tables in support of the ldquofindingsrdquo by scientific researchers is somewhat different
LC Chen at BIME NTU
Three Forms of Data Presentation
Text contrary to what you may have heard not all analyses or results warrant a Table or Figure Some simple results are best stated in a single sentence with data summarized parenthetically
Seed production was higher for plants in the full-sun treatment (523 +-68 seeds) than for those receiving filtered light (147+- 32 seeds t=118 df=55 plt0001)
Tables Tables present or summarize lists of numbers or text in columns each column having a title or label Do not use a table when you wish to show a trend or a pattern of relationship between sets of values - these are better presented in a Figure
Figures Figures are visual presentations of results including graphs diagrams photos drawings schematics maps etc Graphs are the most common type of figure Graphs show trends or patterns of relationship
LC Chen at BIME NTU
How to Refer Tables and Figures
How to refer to Tables and Figures from the text Every Figure and Table included in the paper MUST be referred to from the text Use sentences that draw the readers attention to the relationship or trend you wish to highlight referring to the appropriate Figure or Table only parenthetically
Ex Germination rates were significantly higher after 24 h in running water than in controls (Fig 4)
Ex DNA sequence homologies for the purple gene from the four congeners (Table 1) show high similarity differing by at most 4 base pairs
Avoid sentences that give no information other than directing the reader to the Figure or Table
Ex Table 1 shows the summary results for male and female heights at Bates College
LC Chen at BIME NTU
Numbering Placement amp Foolproof
How to number Tables and Figures Figures and Tables are numbered independently in the sequence in which you refer to them in the text starting with Figure 1 and Table 1
Placement of Figures and Tables within the Paper In consideration of your readers place each Table or Figure as near as possible to the place where you first refer to it (eg the next page) For manuscripts (eg lab papers) Tables and Figures are usually put on separate pages from text material
The Acid Test for Tables and Figures Any Table or Figure you present must be sufficiently clear well-labeled and described by its legend to be understood by your intended audience without reading the results section ie it must be able to stand alone and be interpretable Overly complicated Figures or Tables may be difficult to understand in or out of context so strive for simplicity whenever possible If you are unsure whether your tables or figures meet these criteria give them to a fellow biology major (not in your course) and ask them to interpret your results
LC Chen at BIME NTU
Descriptive Legends and Captions
bull Descriptive Legends or Captions To pass the acid test above a clear and complete legend (sometimes called a caption) is essential Like the title of the paper itself each legend should convey as much information as possible about what the Table or Figure Do not simply restate the axis labels with a versus written in between
bull Example Figure 1 Height frequency () of White Pines (Pinus strobus) at Thorncrag Bird Sanctuary Lewiston Maine before and after the Ice Storm of 98 Before n=137 after n=133 Approximately 16 of canopy pines were topped by heavy ice loads averaging 36 m stem length decrease Four trees fell during the storm and were excluded from the post-storm survey
Where do you place the legend
bull Table legends go above the body of the Table and are left justified Tables are read from the top down
bull Figure legends go below the graph graphs and other types of Figures are usually read from the bottom up
LC Chen at BIME NTU
Table Prep Rule of Thumb
bull Determine journalrsquos limitations on number style or structure of tables
bull Tables should be useful (not too simple) and easy to understand (not too complex)
bull Use tables when exact values are important
bull Use tables when they are more efficient to summarize results than in long complex sentences
bull Tables with footnotes and abbreviations should be easily understandable
bull General rule 1 table per 1000 words of text
LC Chen at BIME NTU
Anatomy of Table
The typical layout of a table in three sections demarcated by lines
LC Chen at BIME NTU
Tables are most easily constructed
using your word processors table
function or a spread sheet such as
Excel Gridlines or boxes commonly
invoked by word processors are
optional for our purposes but
unlikely to be permitted in a journal
LC Chen at BIME NTU
Example Literature Comparison
LC Chen at BIME NTU
units are specified in column headings wherever appropriate
Example Summary of Readings
LC Chen at BIME NTU
Types of illustrations
bull Chartsgraphs (bar graph frequency histogram XY scatter plot XY line graph)
bull Photographs (including X-rays SEM DNAprotein image)
bull Line drawings (schematic illustration instrumentation)
bull Use to provide evidence emphasis or efficiency
bull As with tables data in charts may be easier to understand than long statements of text
bull Figures with legends and abbreviations should be easily understandable
Figure Prep Rule of Thumb
LC Chen at BIME NTU
bull Determine journalrsquos limitations on number size and types of figuresillustrations
bull Black and white vs colorbull Journal may not accept one or the other
bull Journal may charge for use of color
bull Resolution of images typical requirementsbull At least 300 dpi for photographs
bull 1200 dpi for line art
bull May require glossy prints (hard copies) or specific electronic file types
bull Label all figures according to journalrsquos specifications
bull Clearly mark the correct orientation of the photograph
bull Provide figure legends describing what is shown
Figure Preparation (Contrsquod)
LC Chen at BIME NTU
Anatomy of Figures (Graph)
LC Chen at BIME NTU
Anatomy of Figures (Chart)
LC Chen at BIME NTU
Graph Example Bar Chart
LC Chen at BIME NTU
Graph Example Frequency Histogram
LC Chen at BIME NTU
Graph Example X-Y Scatter Plot
LC Chen at BIME NTU
Graph Example X-Y Line Plot
LC Chen at BIME NTU
ScatterLine Plot Curve Fitting
LC Chen at BIME NTU
Photograph Example SEM
LC Chen at BIME NTU
Photograph Example DC Picture
(Before charging) (Charging by PV)
(Fully charged) (Removal of PV)
LC Chen at BIME NTU
0-35 AS
35-70 AS
70-100 AS
Done by TzengFEB 2005
1o Ammonium Sulfate Precipitation
2o Anion Exchange Chromatography
Photograph Example Protein PAGE
LC Chen at BIME NTU
Daytime application an IWECB-
based window can be charged and
colored by a photovoltaic cell
(1) to store solar energy and
(2) to attenuate solar irradiation
Nighttime application an IWECB-
based window can be discharged amp
bleached
(1) to drive low-power devices and
(2) to increase the illumination
Line Drawing Schematic Illustration
LC Chen at BIME NTU
Line Drawing Method and Theory
LC Chen at BIME NTU
Line Drawing Experimental Setup
LC Chen at BIME NTU
01 1 10 100 1000
10x106
10x107
S = SO + S
MAX [A] (K
D + [A]) + k[A]
Ideal model for fitting total bindingK
D = 8 nM k = 7944 K
N ~ O (1 M)
Typ
hoo
n R
eadin
gAptamer No108 Conc (nM)
Surface for TP immobilization
Protein
Aptamer
Biotin
Streptavidin (SA)
Alkaline Phosphatase (AP) Unreacted
Substrate
ReactedSubstrate
Enzyme-Linked Immunosorbent Assay (ELISA)
SA-Biotin interaction Kd ~ 10-15 M = fM ~ ca 260 pNt
Typhoon scan aptamer 5-fold dilution series
Aptamer with nM affinity confirmed
Kd = 8 nM
Combination of Figure Presentation
LC Chen at BIME NTU
Frequently Used Software
bull Word
bull PowerPoint
bull CorelDraw
bull Photoshop
bull Excel
bull Origin
bull Grapher
bull SigmaPlot
bull Othershellip
LC Chen at BIME NTU
LC Chen at BIME NTU
A final and essential step for data presentation consider the efficiency (do things right) and effectiveness (do the right things) Recall the model of ldquoApple Dailyrdquo
If you canrsquot read more papershellip
Can you tell the difference between the figures in a rejected paper and an accepted paper
LC Chen at BIME NTU
同學在論文圖表製作過程中常見違反學術倫理而不自覺的情事
直接剪貼網路( Internet )或文獻( PDF )圖片但未經授權甚至未合理引用 ( cf 原文引用)
製作漂亮數據或使用合成照片以滿足自己的結論
未重複實驗僅報導成功一次的數據
LC Chen at BIME NTU
Essence of Scientific ResearchA valuable thesis is based on the REPRODUCIBLE FACTS AND FIGURES and the most importantly YOUR OWN CREACTIONS NOT NUMBER OF PAGES
httpwwwcartoonstockcomdirectorysscientistasp
LC Chen at BIME NTU
Three Forms of Data Presentation
Text contrary to what you may have heard not all analyses or results warrant a Table or Figure Some simple results are best stated in a single sentence with data summarized parenthetically
Seed production was higher for plants in the full-sun treatment (523 +-68 seeds) than for those receiving filtered light (147+- 32 seeds t=118 df=55 plt0001)
Tables Tables present or summarize lists of numbers or text in columns each column having a title or label Do not use a table when you wish to show a trend or a pattern of relationship between sets of values - these are better presented in a Figure
Figures Figures are visual presentations of results including graphs diagrams photos drawings schematics maps etc Graphs are the most common type of figure Graphs show trends or patterns of relationship
LC Chen at BIME NTU
How to Refer Tables and Figures
How to refer to Tables and Figures from the text Every Figure and Table included in the paper MUST be referred to from the text Use sentences that draw the readers attention to the relationship or trend you wish to highlight referring to the appropriate Figure or Table only parenthetically
Ex Germination rates were significantly higher after 24 h in running water than in controls (Fig 4)
Ex DNA sequence homologies for the purple gene from the four congeners (Table 1) show high similarity differing by at most 4 base pairs
Avoid sentences that give no information other than directing the reader to the Figure or Table
Ex Table 1 shows the summary results for male and female heights at Bates College
LC Chen at BIME NTU
Numbering Placement amp Foolproof
How to number Tables and Figures Figures and Tables are numbered independently in the sequence in which you refer to them in the text starting with Figure 1 and Table 1
Placement of Figures and Tables within the Paper In consideration of your readers place each Table or Figure as near as possible to the place where you first refer to it (eg the next page) For manuscripts (eg lab papers) Tables and Figures are usually put on separate pages from text material
The Acid Test for Tables and Figures Any Table or Figure you present must be sufficiently clear well-labeled and described by its legend to be understood by your intended audience without reading the results section ie it must be able to stand alone and be interpretable Overly complicated Figures or Tables may be difficult to understand in or out of context so strive for simplicity whenever possible If you are unsure whether your tables or figures meet these criteria give them to a fellow biology major (not in your course) and ask them to interpret your results
LC Chen at BIME NTU
Descriptive Legends and Captions
bull Descriptive Legends or Captions To pass the acid test above a clear and complete legend (sometimes called a caption) is essential Like the title of the paper itself each legend should convey as much information as possible about what the Table or Figure Do not simply restate the axis labels with a versus written in between
bull Example Figure 1 Height frequency () of White Pines (Pinus strobus) at Thorncrag Bird Sanctuary Lewiston Maine before and after the Ice Storm of 98 Before n=137 after n=133 Approximately 16 of canopy pines were topped by heavy ice loads averaging 36 m stem length decrease Four trees fell during the storm and were excluded from the post-storm survey
Where do you place the legend
bull Table legends go above the body of the Table and are left justified Tables are read from the top down
bull Figure legends go below the graph graphs and other types of Figures are usually read from the bottom up
LC Chen at BIME NTU
Table Prep Rule of Thumb
bull Determine journalrsquos limitations on number style or structure of tables
bull Tables should be useful (not too simple) and easy to understand (not too complex)
bull Use tables when exact values are important
bull Use tables when they are more efficient to summarize results than in long complex sentences
bull Tables with footnotes and abbreviations should be easily understandable
bull General rule 1 table per 1000 words of text
LC Chen at BIME NTU
Anatomy of Table
The typical layout of a table in three sections demarcated by lines
LC Chen at BIME NTU
Tables are most easily constructed
using your word processors table
function or a spread sheet such as
Excel Gridlines or boxes commonly
invoked by word processors are
optional for our purposes but
unlikely to be permitted in a journal
LC Chen at BIME NTU
Example Literature Comparison
LC Chen at BIME NTU
units are specified in column headings wherever appropriate
Example Summary of Readings
LC Chen at BIME NTU
Types of illustrations
bull Chartsgraphs (bar graph frequency histogram XY scatter plot XY line graph)
bull Photographs (including X-rays SEM DNAprotein image)
bull Line drawings (schematic illustration instrumentation)
bull Use to provide evidence emphasis or efficiency
bull As with tables data in charts may be easier to understand than long statements of text
bull Figures with legends and abbreviations should be easily understandable
Figure Prep Rule of Thumb
LC Chen at BIME NTU
bull Determine journalrsquos limitations on number size and types of figuresillustrations
bull Black and white vs colorbull Journal may not accept one or the other
bull Journal may charge for use of color
bull Resolution of images typical requirementsbull At least 300 dpi for photographs
bull 1200 dpi for line art
bull May require glossy prints (hard copies) or specific electronic file types
bull Label all figures according to journalrsquos specifications
bull Clearly mark the correct orientation of the photograph
bull Provide figure legends describing what is shown
Figure Preparation (Contrsquod)
LC Chen at BIME NTU
Anatomy of Figures (Graph)
LC Chen at BIME NTU
Anatomy of Figures (Chart)
LC Chen at BIME NTU
Graph Example Bar Chart
LC Chen at BIME NTU
Graph Example Frequency Histogram
LC Chen at BIME NTU
Graph Example X-Y Scatter Plot
LC Chen at BIME NTU
Graph Example X-Y Line Plot
LC Chen at BIME NTU
ScatterLine Plot Curve Fitting
LC Chen at BIME NTU
Photograph Example SEM
LC Chen at BIME NTU
Photograph Example DC Picture
(Before charging) (Charging by PV)
(Fully charged) (Removal of PV)
LC Chen at BIME NTU
0-35 AS
35-70 AS
70-100 AS
Done by TzengFEB 2005
1o Ammonium Sulfate Precipitation
2o Anion Exchange Chromatography
Photograph Example Protein PAGE
LC Chen at BIME NTU
Daytime application an IWECB-
based window can be charged and
colored by a photovoltaic cell
(1) to store solar energy and
(2) to attenuate solar irradiation
Nighttime application an IWECB-
based window can be discharged amp
bleached
(1) to drive low-power devices and
(2) to increase the illumination
Line Drawing Schematic Illustration
LC Chen at BIME NTU
Line Drawing Method and Theory
LC Chen at BIME NTU
Line Drawing Experimental Setup
LC Chen at BIME NTU
01 1 10 100 1000
10x106
10x107
S = SO + S
MAX [A] (K
D + [A]) + k[A]
Ideal model for fitting total bindingK
D = 8 nM k = 7944 K
N ~ O (1 M)
Typ
hoo
n R
eadin
gAptamer No108 Conc (nM)
Surface for TP immobilization
Protein
Aptamer
Biotin
Streptavidin (SA)
Alkaline Phosphatase (AP) Unreacted
Substrate
ReactedSubstrate
Enzyme-Linked Immunosorbent Assay (ELISA)
SA-Biotin interaction Kd ~ 10-15 M = fM ~ ca 260 pNt
Typhoon scan aptamer 5-fold dilution series
Aptamer with nM affinity confirmed
Kd = 8 nM
Combination of Figure Presentation
LC Chen at BIME NTU
Frequently Used Software
bull Word
bull PowerPoint
bull CorelDraw
bull Photoshop
bull Excel
bull Origin
bull Grapher
bull SigmaPlot
bull Othershellip
LC Chen at BIME NTU
LC Chen at BIME NTU
A final and essential step for data presentation consider the efficiency (do things right) and effectiveness (do the right things) Recall the model of ldquoApple Dailyrdquo
If you canrsquot read more papershellip
Can you tell the difference between the figures in a rejected paper and an accepted paper
LC Chen at BIME NTU
同學在論文圖表製作過程中常見違反學術倫理而不自覺的情事
直接剪貼網路( Internet )或文獻( PDF )圖片但未經授權甚至未合理引用 ( cf 原文引用)
製作漂亮數據或使用合成照片以滿足自己的結論
未重複實驗僅報導成功一次的數據
LC Chen at BIME NTU
Essence of Scientific ResearchA valuable thesis is based on the REPRODUCIBLE FACTS AND FIGURES and the most importantly YOUR OWN CREACTIONS NOT NUMBER OF PAGES
httpwwwcartoonstockcomdirectorysscientistasp
LC Chen at BIME NTU
How to Refer Tables and Figures
How to refer to Tables and Figures from the text Every Figure and Table included in the paper MUST be referred to from the text Use sentences that draw the readers attention to the relationship or trend you wish to highlight referring to the appropriate Figure or Table only parenthetically
Ex Germination rates were significantly higher after 24 h in running water than in controls (Fig 4)
Ex DNA sequence homologies for the purple gene from the four congeners (Table 1) show high similarity differing by at most 4 base pairs
Avoid sentences that give no information other than directing the reader to the Figure or Table
Ex Table 1 shows the summary results for male and female heights at Bates College
LC Chen at BIME NTU
Numbering Placement amp Foolproof
How to number Tables and Figures Figures and Tables are numbered independently in the sequence in which you refer to them in the text starting with Figure 1 and Table 1
Placement of Figures and Tables within the Paper In consideration of your readers place each Table or Figure as near as possible to the place where you first refer to it (eg the next page) For manuscripts (eg lab papers) Tables and Figures are usually put on separate pages from text material
The Acid Test for Tables and Figures Any Table or Figure you present must be sufficiently clear well-labeled and described by its legend to be understood by your intended audience without reading the results section ie it must be able to stand alone and be interpretable Overly complicated Figures or Tables may be difficult to understand in or out of context so strive for simplicity whenever possible If you are unsure whether your tables or figures meet these criteria give them to a fellow biology major (not in your course) and ask them to interpret your results
LC Chen at BIME NTU
Descriptive Legends and Captions
bull Descriptive Legends or Captions To pass the acid test above a clear and complete legend (sometimes called a caption) is essential Like the title of the paper itself each legend should convey as much information as possible about what the Table or Figure Do not simply restate the axis labels with a versus written in between
bull Example Figure 1 Height frequency () of White Pines (Pinus strobus) at Thorncrag Bird Sanctuary Lewiston Maine before and after the Ice Storm of 98 Before n=137 after n=133 Approximately 16 of canopy pines were topped by heavy ice loads averaging 36 m stem length decrease Four trees fell during the storm and were excluded from the post-storm survey
Where do you place the legend
bull Table legends go above the body of the Table and are left justified Tables are read from the top down
bull Figure legends go below the graph graphs and other types of Figures are usually read from the bottom up
LC Chen at BIME NTU
Table Prep Rule of Thumb
bull Determine journalrsquos limitations on number style or structure of tables
bull Tables should be useful (not too simple) and easy to understand (not too complex)
bull Use tables when exact values are important
bull Use tables when they are more efficient to summarize results than in long complex sentences
bull Tables with footnotes and abbreviations should be easily understandable
bull General rule 1 table per 1000 words of text
LC Chen at BIME NTU
Anatomy of Table
The typical layout of a table in three sections demarcated by lines
LC Chen at BIME NTU
Tables are most easily constructed
using your word processors table
function or a spread sheet such as
Excel Gridlines or boxes commonly
invoked by word processors are
optional for our purposes but
unlikely to be permitted in a journal
LC Chen at BIME NTU
Example Literature Comparison
LC Chen at BIME NTU
units are specified in column headings wherever appropriate
Example Summary of Readings
LC Chen at BIME NTU
Types of illustrations
bull Chartsgraphs (bar graph frequency histogram XY scatter plot XY line graph)
bull Photographs (including X-rays SEM DNAprotein image)
bull Line drawings (schematic illustration instrumentation)
bull Use to provide evidence emphasis or efficiency
bull As with tables data in charts may be easier to understand than long statements of text
bull Figures with legends and abbreviations should be easily understandable
Figure Prep Rule of Thumb
LC Chen at BIME NTU
bull Determine journalrsquos limitations on number size and types of figuresillustrations
bull Black and white vs colorbull Journal may not accept one or the other
bull Journal may charge for use of color
bull Resolution of images typical requirementsbull At least 300 dpi for photographs
bull 1200 dpi for line art
bull May require glossy prints (hard copies) or specific electronic file types
bull Label all figures according to journalrsquos specifications
bull Clearly mark the correct orientation of the photograph
bull Provide figure legends describing what is shown
Figure Preparation (Contrsquod)
LC Chen at BIME NTU
Anatomy of Figures (Graph)
LC Chen at BIME NTU
Anatomy of Figures (Chart)
LC Chen at BIME NTU
Graph Example Bar Chart
LC Chen at BIME NTU
Graph Example Frequency Histogram
LC Chen at BIME NTU
Graph Example X-Y Scatter Plot
LC Chen at BIME NTU
Graph Example X-Y Line Plot
LC Chen at BIME NTU
ScatterLine Plot Curve Fitting
LC Chen at BIME NTU
Photograph Example SEM
LC Chen at BIME NTU
Photograph Example DC Picture
(Before charging) (Charging by PV)
(Fully charged) (Removal of PV)
LC Chen at BIME NTU
0-35 AS
35-70 AS
70-100 AS
Done by TzengFEB 2005
1o Ammonium Sulfate Precipitation
2o Anion Exchange Chromatography
Photograph Example Protein PAGE
LC Chen at BIME NTU
Daytime application an IWECB-
based window can be charged and
colored by a photovoltaic cell
(1) to store solar energy and
(2) to attenuate solar irradiation
Nighttime application an IWECB-
based window can be discharged amp
bleached
(1) to drive low-power devices and
(2) to increase the illumination
Line Drawing Schematic Illustration
LC Chen at BIME NTU
Line Drawing Method and Theory
LC Chen at BIME NTU
Line Drawing Experimental Setup
LC Chen at BIME NTU
01 1 10 100 1000
10x106
10x107
S = SO + S
MAX [A] (K
D + [A]) + k[A]
Ideal model for fitting total bindingK
D = 8 nM k = 7944 K
N ~ O (1 M)
Typ
hoo
n R
eadin
gAptamer No108 Conc (nM)
Surface for TP immobilization
Protein
Aptamer
Biotin
Streptavidin (SA)
Alkaline Phosphatase (AP) Unreacted
Substrate
ReactedSubstrate
Enzyme-Linked Immunosorbent Assay (ELISA)
SA-Biotin interaction Kd ~ 10-15 M = fM ~ ca 260 pNt
Typhoon scan aptamer 5-fold dilution series
Aptamer with nM affinity confirmed
Kd = 8 nM
Combination of Figure Presentation
LC Chen at BIME NTU
Frequently Used Software
bull Word
bull PowerPoint
bull CorelDraw
bull Photoshop
bull Excel
bull Origin
bull Grapher
bull SigmaPlot
bull Othershellip
LC Chen at BIME NTU
LC Chen at BIME NTU
A final and essential step for data presentation consider the efficiency (do things right) and effectiveness (do the right things) Recall the model of ldquoApple Dailyrdquo
If you canrsquot read more papershellip
Can you tell the difference between the figures in a rejected paper and an accepted paper
LC Chen at BIME NTU
同學在論文圖表製作過程中常見違反學術倫理而不自覺的情事
直接剪貼網路( Internet )或文獻( PDF )圖片但未經授權甚至未合理引用 ( cf 原文引用)
製作漂亮數據或使用合成照片以滿足自己的結論
未重複實驗僅報導成功一次的數據
LC Chen at BIME NTU
Essence of Scientific ResearchA valuable thesis is based on the REPRODUCIBLE FACTS AND FIGURES and the most importantly YOUR OWN CREACTIONS NOT NUMBER OF PAGES
httpwwwcartoonstockcomdirectorysscientistasp
LC Chen at BIME NTU
Numbering Placement amp Foolproof
How to number Tables and Figures Figures and Tables are numbered independently in the sequence in which you refer to them in the text starting with Figure 1 and Table 1
Placement of Figures and Tables within the Paper In consideration of your readers place each Table or Figure as near as possible to the place where you first refer to it (eg the next page) For manuscripts (eg lab papers) Tables and Figures are usually put on separate pages from text material
The Acid Test for Tables and Figures Any Table or Figure you present must be sufficiently clear well-labeled and described by its legend to be understood by your intended audience without reading the results section ie it must be able to stand alone and be interpretable Overly complicated Figures or Tables may be difficult to understand in or out of context so strive for simplicity whenever possible If you are unsure whether your tables or figures meet these criteria give them to a fellow biology major (not in your course) and ask them to interpret your results
LC Chen at BIME NTU
Descriptive Legends and Captions
bull Descriptive Legends or Captions To pass the acid test above a clear and complete legend (sometimes called a caption) is essential Like the title of the paper itself each legend should convey as much information as possible about what the Table or Figure Do not simply restate the axis labels with a versus written in between
bull Example Figure 1 Height frequency () of White Pines (Pinus strobus) at Thorncrag Bird Sanctuary Lewiston Maine before and after the Ice Storm of 98 Before n=137 after n=133 Approximately 16 of canopy pines were topped by heavy ice loads averaging 36 m stem length decrease Four trees fell during the storm and were excluded from the post-storm survey
Where do you place the legend
bull Table legends go above the body of the Table and are left justified Tables are read from the top down
bull Figure legends go below the graph graphs and other types of Figures are usually read from the bottom up
LC Chen at BIME NTU
Table Prep Rule of Thumb
bull Determine journalrsquos limitations on number style or structure of tables
bull Tables should be useful (not too simple) and easy to understand (not too complex)
bull Use tables when exact values are important
bull Use tables when they are more efficient to summarize results than in long complex sentences
bull Tables with footnotes and abbreviations should be easily understandable
bull General rule 1 table per 1000 words of text
LC Chen at BIME NTU
Anatomy of Table
The typical layout of a table in three sections demarcated by lines
LC Chen at BIME NTU
Tables are most easily constructed
using your word processors table
function or a spread sheet such as
Excel Gridlines or boxes commonly
invoked by word processors are
optional for our purposes but
unlikely to be permitted in a journal
LC Chen at BIME NTU
Example Literature Comparison
LC Chen at BIME NTU
units are specified in column headings wherever appropriate
Example Summary of Readings
LC Chen at BIME NTU
Types of illustrations
bull Chartsgraphs (bar graph frequency histogram XY scatter plot XY line graph)
bull Photographs (including X-rays SEM DNAprotein image)
bull Line drawings (schematic illustration instrumentation)
bull Use to provide evidence emphasis or efficiency
bull As with tables data in charts may be easier to understand than long statements of text
bull Figures with legends and abbreviations should be easily understandable
Figure Prep Rule of Thumb
LC Chen at BIME NTU
bull Determine journalrsquos limitations on number size and types of figuresillustrations
bull Black and white vs colorbull Journal may not accept one or the other
bull Journal may charge for use of color
bull Resolution of images typical requirementsbull At least 300 dpi for photographs
bull 1200 dpi for line art
bull May require glossy prints (hard copies) or specific electronic file types
bull Label all figures according to journalrsquos specifications
bull Clearly mark the correct orientation of the photograph
bull Provide figure legends describing what is shown
Figure Preparation (Contrsquod)
LC Chen at BIME NTU
Anatomy of Figures (Graph)
LC Chen at BIME NTU
Anatomy of Figures (Chart)
LC Chen at BIME NTU
Graph Example Bar Chart
LC Chen at BIME NTU
Graph Example Frequency Histogram
LC Chen at BIME NTU
Graph Example X-Y Scatter Plot
LC Chen at BIME NTU
Graph Example X-Y Line Plot
LC Chen at BIME NTU
ScatterLine Plot Curve Fitting
LC Chen at BIME NTU
Photograph Example SEM
LC Chen at BIME NTU
Photograph Example DC Picture
(Before charging) (Charging by PV)
(Fully charged) (Removal of PV)
LC Chen at BIME NTU
0-35 AS
35-70 AS
70-100 AS
Done by TzengFEB 2005
1o Ammonium Sulfate Precipitation
2o Anion Exchange Chromatography
Photograph Example Protein PAGE
LC Chen at BIME NTU
Daytime application an IWECB-
based window can be charged and
colored by a photovoltaic cell
(1) to store solar energy and
(2) to attenuate solar irradiation
Nighttime application an IWECB-
based window can be discharged amp
bleached
(1) to drive low-power devices and
(2) to increase the illumination
Line Drawing Schematic Illustration
LC Chen at BIME NTU
Line Drawing Method and Theory
LC Chen at BIME NTU
Line Drawing Experimental Setup
LC Chen at BIME NTU
01 1 10 100 1000
10x106
10x107
S = SO + S
MAX [A] (K
D + [A]) + k[A]
Ideal model for fitting total bindingK
D = 8 nM k = 7944 K
N ~ O (1 M)
Typ
hoo
n R
eadin
gAptamer No108 Conc (nM)
Surface for TP immobilization
Protein
Aptamer
Biotin
Streptavidin (SA)
Alkaline Phosphatase (AP) Unreacted
Substrate
ReactedSubstrate
Enzyme-Linked Immunosorbent Assay (ELISA)
SA-Biotin interaction Kd ~ 10-15 M = fM ~ ca 260 pNt
Typhoon scan aptamer 5-fold dilution series
Aptamer with nM affinity confirmed
Kd = 8 nM
Combination of Figure Presentation
LC Chen at BIME NTU
Frequently Used Software
bull Word
bull PowerPoint
bull CorelDraw
bull Photoshop
bull Excel
bull Origin
bull Grapher
bull SigmaPlot
bull Othershellip
LC Chen at BIME NTU
LC Chen at BIME NTU
A final and essential step for data presentation consider the efficiency (do things right) and effectiveness (do the right things) Recall the model of ldquoApple Dailyrdquo
If you canrsquot read more papershellip
Can you tell the difference between the figures in a rejected paper and an accepted paper
LC Chen at BIME NTU
同學在論文圖表製作過程中常見違反學術倫理而不自覺的情事
直接剪貼網路( Internet )或文獻( PDF )圖片但未經授權甚至未合理引用 ( cf 原文引用)
製作漂亮數據或使用合成照片以滿足自己的結論
未重複實驗僅報導成功一次的數據
LC Chen at BIME NTU
Essence of Scientific ResearchA valuable thesis is based on the REPRODUCIBLE FACTS AND FIGURES and the most importantly YOUR OWN CREACTIONS NOT NUMBER OF PAGES
httpwwwcartoonstockcomdirectorysscientistasp
LC Chen at BIME NTU
Descriptive Legends and Captions
bull Descriptive Legends or Captions To pass the acid test above a clear and complete legend (sometimes called a caption) is essential Like the title of the paper itself each legend should convey as much information as possible about what the Table or Figure Do not simply restate the axis labels with a versus written in between
bull Example Figure 1 Height frequency () of White Pines (Pinus strobus) at Thorncrag Bird Sanctuary Lewiston Maine before and after the Ice Storm of 98 Before n=137 after n=133 Approximately 16 of canopy pines were topped by heavy ice loads averaging 36 m stem length decrease Four trees fell during the storm and were excluded from the post-storm survey
Where do you place the legend
bull Table legends go above the body of the Table and are left justified Tables are read from the top down
bull Figure legends go below the graph graphs and other types of Figures are usually read from the bottom up
LC Chen at BIME NTU
Table Prep Rule of Thumb
bull Determine journalrsquos limitations on number style or structure of tables
bull Tables should be useful (not too simple) and easy to understand (not too complex)
bull Use tables when exact values are important
bull Use tables when they are more efficient to summarize results than in long complex sentences
bull Tables with footnotes and abbreviations should be easily understandable
bull General rule 1 table per 1000 words of text
LC Chen at BIME NTU
Anatomy of Table
The typical layout of a table in three sections demarcated by lines
LC Chen at BIME NTU
Tables are most easily constructed
using your word processors table
function or a spread sheet such as
Excel Gridlines or boxes commonly
invoked by word processors are
optional for our purposes but
unlikely to be permitted in a journal
LC Chen at BIME NTU
Example Literature Comparison
LC Chen at BIME NTU
units are specified in column headings wherever appropriate
Example Summary of Readings
LC Chen at BIME NTU
Types of illustrations
bull Chartsgraphs (bar graph frequency histogram XY scatter plot XY line graph)
bull Photographs (including X-rays SEM DNAprotein image)
bull Line drawings (schematic illustration instrumentation)
bull Use to provide evidence emphasis or efficiency
bull As with tables data in charts may be easier to understand than long statements of text
bull Figures with legends and abbreviations should be easily understandable
Figure Prep Rule of Thumb
LC Chen at BIME NTU
bull Determine journalrsquos limitations on number size and types of figuresillustrations
bull Black and white vs colorbull Journal may not accept one or the other
bull Journal may charge for use of color
bull Resolution of images typical requirementsbull At least 300 dpi for photographs
bull 1200 dpi for line art
bull May require glossy prints (hard copies) or specific electronic file types
bull Label all figures according to journalrsquos specifications
bull Clearly mark the correct orientation of the photograph
bull Provide figure legends describing what is shown
Figure Preparation (Contrsquod)
LC Chen at BIME NTU
Anatomy of Figures (Graph)
LC Chen at BIME NTU
Anatomy of Figures (Chart)
LC Chen at BIME NTU
Graph Example Bar Chart
LC Chen at BIME NTU
Graph Example Frequency Histogram
LC Chen at BIME NTU
Graph Example X-Y Scatter Plot
LC Chen at BIME NTU
Graph Example X-Y Line Plot
LC Chen at BIME NTU
ScatterLine Plot Curve Fitting
LC Chen at BIME NTU
Photograph Example SEM
LC Chen at BIME NTU
Photograph Example DC Picture
(Before charging) (Charging by PV)
(Fully charged) (Removal of PV)
LC Chen at BIME NTU
0-35 AS
35-70 AS
70-100 AS
Done by TzengFEB 2005
1o Ammonium Sulfate Precipitation
2o Anion Exchange Chromatography
Photograph Example Protein PAGE
LC Chen at BIME NTU
Daytime application an IWECB-
based window can be charged and
colored by a photovoltaic cell
(1) to store solar energy and
(2) to attenuate solar irradiation
Nighttime application an IWECB-
based window can be discharged amp
bleached
(1) to drive low-power devices and
(2) to increase the illumination
Line Drawing Schematic Illustration
LC Chen at BIME NTU
Line Drawing Method and Theory
LC Chen at BIME NTU
Line Drawing Experimental Setup
LC Chen at BIME NTU
01 1 10 100 1000
10x106
10x107
S = SO + S
MAX [A] (K
D + [A]) + k[A]
Ideal model for fitting total bindingK
D = 8 nM k = 7944 K
N ~ O (1 M)
Typ
hoo
n R
eadin
gAptamer No108 Conc (nM)
Surface for TP immobilization
Protein
Aptamer
Biotin
Streptavidin (SA)
Alkaline Phosphatase (AP) Unreacted
Substrate
ReactedSubstrate
Enzyme-Linked Immunosorbent Assay (ELISA)
SA-Biotin interaction Kd ~ 10-15 M = fM ~ ca 260 pNt
Typhoon scan aptamer 5-fold dilution series
Aptamer with nM affinity confirmed
Kd = 8 nM
Combination of Figure Presentation
LC Chen at BIME NTU
Frequently Used Software
bull Word
bull PowerPoint
bull CorelDraw
bull Photoshop
bull Excel
bull Origin
bull Grapher
bull SigmaPlot
bull Othershellip
LC Chen at BIME NTU
LC Chen at BIME NTU
A final and essential step for data presentation consider the efficiency (do things right) and effectiveness (do the right things) Recall the model of ldquoApple Dailyrdquo
If you canrsquot read more papershellip
Can you tell the difference between the figures in a rejected paper and an accepted paper
LC Chen at BIME NTU
同學在論文圖表製作過程中常見違反學術倫理而不自覺的情事
直接剪貼網路( Internet )或文獻( PDF )圖片但未經授權甚至未合理引用 ( cf 原文引用)
製作漂亮數據或使用合成照片以滿足自己的結論
未重複實驗僅報導成功一次的數據
LC Chen at BIME NTU
Essence of Scientific ResearchA valuable thesis is based on the REPRODUCIBLE FACTS AND FIGURES and the most importantly YOUR OWN CREACTIONS NOT NUMBER OF PAGES
httpwwwcartoonstockcomdirectorysscientistasp
LC Chen at BIME NTU
Table Prep Rule of Thumb
bull Determine journalrsquos limitations on number style or structure of tables
bull Tables should be useful (not too simple) and easy to understand (not too complex)
bull Use tables when exact values are important
bull Use tables when they are more efficient to summarize results than in long complex sentences
bull Tables with footnotes and abbreviations should be easily understandable
bull General rule 1 table per 1000 words of text
LC Chen at BIME NTU
Anatomy of Table
The typical layout of a table in three sections demarcated by lines
LC Chen at BIME NTU
Tables are most easily constructed
using your word processors table
function or a spread sheet such as
Excel Gridlines or boxes commonly
invoked by word processors are
optional for our purposes but
unlikely to be permitted in a journal
LC Chen at BIME NTU
Example Literature Comparison
LC Chen at BIME NTU
units are specified in column headings wherever appropriate
Example Summary of Readings
LC Chen at BIME NTU
Types of illustrations
bull Chartsgraphs (bar graph frequency histogram XY scatter plot XY line graph)
bull Photographs (including X-rays SEM DNAprotein image)
bull Line drawings (schematic illustration instrumentation)
bull Use to provide evidence emphasis or efficiency
bull As with tables data in charts may be easier to understand than long statements of text
bull Figures with legends and abbreviations should be easily understandable
Figure Prep Rule of Thumb
LC Chen at BIME NTU
bull Determine journalrsquos limitations on number size and types of figuresillustrations
bull Black and white vs colorbull Journal may not accept one or the other
bull Journal may charge for use of color
bull Resolution of images typical requirementsbull At least 300 dpi for photographs
bull 1200 dpi for line art
bull May require glossy prints (hard copies) or specific electronic file types
bull Label all figures according to journalrsquos specifications
bull Clearly mark the correct orientation of the photograph
bull Provide figure legends describing what is shown
Figure Preparation (Contrsquod)
LC Chen at BIME NTU
Anatomy of Figures (Graph)
LC Chen at BIME NTU
Anatomy of Figures (Chart)
LC Chen at BIME NTU
Graph Example Bar Chart
LC Chen at BIME NTU
Graph Example Frequency Histogram
LC Chen at BIME NTU
Graph Example X-Y Scatter Plot
LC Chen at BIME NTU
Graph Example X-Y Line Plot
LC Chen at BIME NTU
ScatterLine Plot Curve Fitting
LC Chen at BIME NTU
Photograph Example SEM
LC Chen at BIME NTU
Photograph Example DC Picture
(Before charging) (Charging by PV)
(Fully charged) (Removal of PV)
LC Chen at BIME NTU
0-35 AS
35-70 AS
70-100 AS
Done by TzengFEB 2005
1o Ammonium Sulfate Precipitation
2o Anion Exchange Chromatography
Photograph Example Protein PAGE
LC Chen at BIME NTU
Daytime application an IWECB-
based window can be charged and
colored by a photovoltaic cell
(1) to store solar energy and
(2) to attenuate solar irradiation
Nighttime application an IWECB-
based window can be discharged amp
bleached
(1) to drive low-power devices and
(2) to increase the illumination
Line Drawing Schematic Illustration
LC Chen at BIME NTU
Line Drawing Method and Theory
LC Chen at BIME NTU
Line Drawing Experimental Setup
LC Chen at BIME NTU
01 1 10 100 1000
10x106
10x107
S = SO + S
MAX [A] (K
D + [A]) + k[A]
Ideal model for fitting total bindingK
D = 8 nM k = 7944 K
N ~ O (1 M)
Typ
hoo
n R
eadin
gAptamer No108 Conc (nM)
Surface for TP immobilization
Protein
Aptamer
Biotin
Streptavidin (SA)
Alkaline Phosphatase (AP) Unreacted
Substrate
ReactedSubstrate
Enzyme-Linked Immunosorbent Assay (ELISA)
SA-Biotin interaction Kd ~ 10-15 M = fM ~ ca 260 pNt
Typhoon scan aptamer 5-fold dilution series
Aptamer with nM affinity confirmed
Kd = 8 nM
Combination of Figure Presentation
LC Chen at BIME NTU
Frequently Used Software
bull Word
bull PowerPoint
bull CorelDraw
bull Photoshop
bull Excel
bull Origin
bull Grapher
bull SigmaPlot
bull Othershellip
LC Chen at BIME NTU
LC Chen at BIME NTU
A final and essential step for data presentation consider the efficiency (do things right) and effectiveness (do the right things) Recall the model of ldquoApple Dailyrdquo
If you canrsquot read more papershellip
Can you tell the difference between the figures in a rejected paper and an accepted paper
LC Chen at BIME NTU
同學在論文圖表製作過程中常見違反學術倫理而不自覺的情事
直接剪貼網路( Internet )或文獻( PDF )圖片但未經授權甚至未合理引用 ( cf 原文引用)
製作漂亮數據或使用合成照片以滿足自己的結論
未重複實驗僅報導成功一次的數據
LC Chen at BIME NTU
Essence of Scientific ResearchA valuable thesis is based on the REPRODUCIBLE FACTS AND FIGURES and the most importantly YOUR OWN CREACTIONS NOT NUMBER OF PAGES
httpwwwcartoonstockcomdirectorysscientistasp
LC Chen at BIME NTU
Anatomy of Table
The typical layout of a table in three sections demarcated by lines
LC Chen at BIME NTU
Tables are most easily constructed
using your word processors table
function or a spread sheet such as
Excel Gridlines or boxes commonly
invoked by word processors are
optional for our purposes but
unlikely to be permitted in a journal
LC Chen at BIME NTU
Example Literature Comparison
LC Chen at BIME NTU
units are specified in column headings wherever appropriate
Example Summary of Readings
LC Chen at BIME NTU
Types of illustrations
bull Chartsgraphs (bar graph frequency histogram XY scatter plot XY line graph)
bull Photographs (including X-rays SEM DNAprotein image)
bull Line drawings (schematic illustration instrumentation)
bull Use to provide evidence emphasis or efficiency
bull As with tables data in charts may be easier to understand than long statements of text
bull Figures with legends and abbreviations should be easily understandable
Figure Prep Rule of Thumb
LC Chen at BIME NTU
bull Determine journalrsquos limitations on number size and types of figuresillustrations
bull Black and white vs colorbull Journal may not accept one or the other
bull Journal may charge for use of color
bull Resolution of images typical requirementsbull At least 300 dpi for photographs
bull 1200 dpi for line art
bull May require glossy prints (hard copies) or specific electronic file types
bull Label all figures according to journalrsquos specifications
bull Clearly mark the correct orientation of the photograph
bull Provide figure legends describing what is shown
Figure Preparation (Contrsquod)
LC Chen at BIME NTU
Anatomy of Figures (Graph)
LC Chen at BIME NTU
Anatomy of Figures (Chart)
LC Chen at BIME NTU
Graph Example Bar Chart
LC Chen at BIME NTU
Graph Example Frequency Histogram
LC Chen at BIME NTU
Graph Example X-Y Scatter Plot
LC Chen at BIME NTU
Graph Example X-Y Line Plot
LC Chen at BIME NTU
ScatterLine Plot Curve Fitting
LC Chen at BIME NTU
Photograph Example SEM
LC Chen at BIME NTU
Photograph Example DC Picture
(Before charging) (Charging by PV)
(Fully charged) (Removal of PV)
LC Chen at BIME NTU
0-35 AS
35-70 AS
70-100 AS
Done by TzengFEB 2005
1o Ammonium Sulfate Precipitation
2o Anion Exchange Chromatography
Photograph Example Protein PAGE
LC Chen at BIME NTU
Daytime application an IWECB-
based window can be charged and
colored by a photovoltaic cell
(1) to store solar energy and
(2) to attenuate solar irradiation
Nighttime application an IWECB-
based window can be discharged amp
bleached
(1) to drive low-power devices and
(2) to increase the illumination
Line Drawing Schematic Illustration
LC Chen at BIME NTU
Line Drawing Method and Theory
LC Chen at BIME NTU
Line Drawing Experimental Setup
LC Chen at BIME NTU
01 1 10 100 1000
10x106
10x107
S = SO + S
MAX [A] (K
D + [A]) + k[A]
Ideal model for fitting total bindingK
D = 8 nM k = 7944 K
N ~ O (1 M)
Typ
hoo
n R
eadin
gAptamer No108 Conc (nM)
Surface for TP immobilization
Protein
Aptamer
Biotin
Streptavidin (SA)
Alkaline Phosphatase (AP) Unreacted
Substrate
ReactedSubstrate
Enzyme-Linked Immunosorbent Assay (ELISA)
SA-Biotin interaction Kd ~ 10-15 M = fM ~ ca 260 pNt
Typhoon scan aptamer 5-fold dilution series
Aptamer with nM affinity confirmed
Kd = 8 nM
Combination of Figure Presentation
LC Chen at BIME NTU
Frequently Used Software
bull Word
bull PowerPoint
bull CorelDraw
bull Photoshop
bull Excel
bull Origin
bull Grapher
bull SigmaPlot
bull Othershellip
LC Chen at BIME NTU
LC Chen at BIME NTU
A final and essential step for data presentation consider the efficiency (do things right) and effectiveness (do the right things) Recall the model of ldquoApple Dailyrdquo
If you canrsquot read more papershellip
Can you tell the difference between the figures in a rejected paper and an accepted paper
LC Chen at BIME NTU
同學在論文圖表製作過程中常見違反學術倫理而不自覺的情事
直接剪貼網路( Internet )或文獻( PDF )圖片但未經授權甚至未合理引用 ( cf 原文引用)
製作漂亮數據或使用合成照片以滿足自己的結論
未重複實驗僅報導成功一次的數據
LC Chen at BIME NTU
Essence of Scientific ResearchA valuable thesis is based on the REPRODUCIBLE FACTS AND FIGURES and the most importantly YOUR OWN CREACTIONS NOT NUMBER OF PAGES
httpwwwcartoonstockcomdirectorysscientistasp
LC Chen at BIME NTU
Tables are most easily constructed
using your word processors table
function or a spread sheet such as
Excel Gridlines or boxes commonly
invoked by word processors are
optional for our purposes but
unlikely to be permitted in a journal
LC Chen at BIME NTU
Example Literature Comparison
LC Chen at BIME NTU
units are specified in column headings wherever appropriate
Example Summary of Readings
LC Chen at BIME NTU
Types of illustrations
bull Chartsgraphs (bar graph frequency histogram XY scatter plot XY line graph)
bull Photographs (including X-rays SEM DNAprotein image)
bull Line drawings (schematic illustration instrumentation)
bull Use to provide evidence emphasis or efficiency
bull As with tables data in charts may be easier to understand than long statements of text
bull Figures with legends and abbreviations should be easily understandable
Figure Prep Rule of Thumb
LC Chen at BIME NTU
bull Determine journalrsquos limitations on number size and types of figuresillustrations
bull Black and white vs colorbull Journal may not accept one or the other
bull Journal may charge for use of color
bull Resolution of images typical requirementsbull At least 300 dpi for photographs
bull 1200 dpi for line art
bull May require glossy prints (hard copies) or specific electronic file types
bull Label all figures according to journalrsquos specifications
bull Clearly mark the correct orientation of the photograph
bull Provide figure legends describing what is shown
Figure Preparation (Contrsquod)
LC Chen at BIME NTU
Anatomy of Figures (Graph)
LC Chen at BIME NTU
Anatomy of Figures (Chart)
LC Chen at BIME NTU
Graph Example Bar Chart
LC Chen at BIME NTU
Graph Example Frequency Histogram
LC Chen at BIME NTU
Graph Example X-Y Scatter Plot
LC Chen at BIME NTU
Graph Example X-Y Line Plot
LC Chen at BIME NTU
ScatterLine Plot Curve Fitting
LC Chen at BIME NTU
Photograph Example SEM
LC Chen at BIME NTU
Photograph Example DC Picture
(Before charging) (Charging by PV)
(Fully charged) (Removal of PV)
LC Chen at BIME NTU
0-35 AS
35-70 AS
70-100 AS
Done by TzengFEB 2005
1o Ammonium Sulfate Precipitation
2o Anion Exchange Chromatography
Photograph Example Protein PAGE
LC Chen at BIME NTU
Daytime application an IWECB-
based window can be charged and
colored by a photovoltaic cell
(1) to store solar energy and
(2) to attenuate solar irradiation
Nighttime application an IWECB-
based window can be discharged amp
bleached
(1) to drive low-power devices and
(2) to increase the illumination
Line Drawing Schematic Illustration
LC Chen at BIME NTU
Line Drawing Method and Theory
LC Chen at BIME NTU
Line Drawing Experimental Setup
LC Chen at BIME NTU
01 1 10 100 1000
10x106
10x107
S = SO + S
MAX [A] (K
D + [A]) + k[A]
Ideal model for fitting total bindingK
D = 8 nM k = 7944 K
N ~ O (1 M)
Typ
hoo
n R
eadin
gAptamer No108 Conc (nM)
Surface for TP immobilization
Protein
Aptamer
Biotin
Streptavidin (SA)
Alkaline Phosphatase (AP) Unreacted
Substrate
ReactedSubstrate
Enzyme-Linked Immunosorbent Assay (ELISA)
SA-Biotin interaction Kd ~ 10-15 M = fM ~ ca 260 pNt
Typhoon scan aptamer 5-fold dilution series
Aptamer with nM affinity confirmed
Kd = 8 nM
Combination of Figure Presentation
LC Chen at BIME NTU
Frequently Used Software
bull Word
bull PowerPoint
bull CorelDraw
bull Photoshop
bull Excel
bull Origin
bull Grapher
bull SigmaPlot
bull Othershellip
LC Chen at BIME NTU
LC Chen at BIME NTU
A final and essential step for data presentation consider the efficiency (do things right) and effectiveness (do the right things) Recall the model of ldquoApple Dailyrdquo
If you canrsquot read more papershellip
Can you tell the difference between the figures in a rejected paper and an accepted paper
LC Chen at BIME NTU
同學在論文圖表製作過程中常見違反學術倫理而不自覺的情事
直接剪貼網路( Internet )或文獻( PDF )圖片但未經授權甚至未合理引用 ( cf 原文引用)
製作漂亮數據或使用合成照片以滿足自己的結論
未重複實驗僅報導成功一次的數據
LC Chen at BIME NTU
Essence of Scientific ResearchA valuable thesis is based on the REPRODUCIBLE FACTS AND FIGURES and the most importantly YOUR OWN CREACTIONS NOT NUMBER OF PAGES
httpwwwcartoonstockcomdirectorysscientistasp
LC Chen at BIME NTU
Example Literature Comparison
LC Chen at BIME NTU
units are specified in column headings wherever appropriate
Example Summary of Readings
LC Chen at BIME NTU
Types of illustrations
bull Chartsgraphs (bar graph frequency histogram XY scatter plot XY line graph)
bull Photographs (including X-rays SEM DNAprotein image)
bull Line drawings (schematic illustration instrumentation)
bull Use to provide evidence emphasis or efficiency
bull As with tables data in charts may be easier to understand than long statements of text
bull Figures with legends and abbreviations should be easily understandable
Figure Prep Rule of Thumb
LC Chen at BIME NTU
bull Determine journalrsquos limitations on number size and types of figuresillustrations
bull Black and white vs colorbull Journal may not accept one or the other
bull Journal may charge for use of color
bull Resolution of images typical requirementsbull At least 300 dpi for photographs
bull 1200 dpi for line art
bull May require glossy prints (hard copies) or specific electronic file types
bull Label all figures according to journalrsquos specifications
bull Clearly mark the correct orientation of the photograph
bull Provide figure legends describing what is shown
Figure Preparation (Contrsquod)
LC Chen at BIME NTU
Anatomy of Figures (Graph)
LC Chen at BIME NTU
Anatomy of Figures (Chart)
LC Chen at BIME NTU
Graph Example Bar Chart
LC Chen at BIME NTU
Graph Example Frequency Histogram
LC Chen at BIME NTU
Graph Example X-Y Scatter Plot
LC Chen at BIME NTU
Graph Example X-Y Line Plot
LC Chen at BIME NTU
ScatterLine Plot Curve Fitting
LC Chen at BIME NTU
Photograph Example SEM
LC Chen at BIME NTU
Photograph Example DC Picture
(Before charging) (Charging by PV)
(Fully charged) (Removal of PV)
LC Chen at BIME NTU
0-35 AS
35-70 AS
70-100 AS
Done by TzengFEB 2005
1o Ammonium Sulfate Precipitation
2o Anion Exchange Chromatography
Photograph Example Protein PAGE
LC Chen at BIME NTU
Daytime application an IWECB-
based window can be charged and
colored by a photovoltaic cell
(1) to store solar energy and
(2) to attenuate solar irradiation
Nighttime application an IWECB-
based window can be discharged amp
bleached
(1) to drive low-power devices and
(2) to increase the illumination
Line Drawing Schematic Illustration
LC Chen at BIME NTU
Line Drawing Method and Theory
LC Chen at BIME NTU
Line Drawing Experimental Setup
LC Chen at BIME NTU
01 1 10 100 1000
10x106
10x107
S = SO + S
MAX [A] (K
D + [A]) + k[A]
Ideal model for fitting total bindingK
D = 8 nM k = 7944 K
N ~ O (1 M)
Typ
hoo
n R
eadin
gAptamer No108 Conc (nM)
Surface for TP immobilization
Protein
Aptamer
Biotin
Streptavidin (SA)
Alkaline Phosphatase (AP) Unreacted
Substrate
ReactedSubstrate
Enzyme-Linked Immunosorbent Assay (ELISA)
SA-Biotin interaction Kd ~ 10-15 M = fM ~ ca 260 pNt
Typhoon scan aptamer 5-fold dilution series
Aptamer with nM affinity confirmed
Kd = 8 nM
Combination of Figure Presentation
LC Chen at BIME NTU
Frequently Used Software
bull Word
bull PowerPoint
bull CorelDraw
bull Photoshop
bull Excel
bull Origin
bull Grapher
bull SigmaPlot
bull Othershellip
LC Chen at BIME NTU
LC Chen at BIME NTU
A final and essential step for data presentation consider the efficiency (do things right) and effectiveness (do the right things) Recall the model of ldquoApple Dailyrdquo
If you canrsquot read more papershellip
Can you tell the difference between the figures in a rejected paper and an accepted paper
LC Chen at BIME NTU
同學在論文圖表製作過程中常見違反學術倫理而不自覺的情事
直接剪貼網路( Internet )或文獻( PDF )圖片但未經授權甚至未合理引用 ( cf 原文引用)
製作漂亮數據或使用合成照片以滿足自己的結論
未重複實驗僅報導成功一次的數據
LC Chen at BIME NTU
Essence of Scientific ResearchA valuable thesis is based on the REPRODUCIBLE FACTS AND FIGURES and the most importantly YOUR OWN CREACTIONS NOT NUMBER OF PAGES
httpwwwcartoonstockcomdirectorysscientistasp
LC Chen at BIME NTU
units are specified in column headings wherever appropriate
Example Summary of Readings
LC Chen at BIME NTU
Types of illustrations
bull Chartsgraphs (bar graph frequency histogram XY scatter plot XY line graph)
bull Photographs (including X-rays SEM DNAprotein image)
bull Line drawings (schematic illustration instrumentation)
bull Use to provide evidence emphasis or efficiency
bull As with tables data in charts may be easier to understand than long statements of text
bull Figures with legends and abbreviations should be easily understandable
Figure Prep Rule of Thumb
LC Chen at BIME NTU
bull Determine journalrsquos limitations on number size and types of figuresillustrations
bull Black and white vs colorbull Journal may not accept one or the other
bull Journal may charge for use of color
bull Resolution of images typical requirementsbull At least 300 dpi for photographs
bull 1200 dpi for line art
bull May require glossy prints (hard copies) or specific electronic file types
bull Label all figures according to journalrsquos specifications
bull Clearly mark the correct orientation of the photograph
bull Provide figure legends describing what is shown
Figure Preparation (Contrsquod)
LC Chen at BIME NTU
Anatomy of Figures (Graph)
LC Chen at BIME NTU
Anatomy of Figures (Chart)
LC Chen at BIME NTU
Graph Example Bar Chart
LC Chen at BIME NTU
Graph Example Frequency Histogram
LC Chen at BIME NTU
Graph Example X-Y Scatter Plot
LC Chen at BIME NTU
Graph Example X-Y Line Plot
LC Chen at BIME NTU
ScatterLine Plot Curve Fitting
LC Chen at BIME NTU
Photograph Example SEM
LC Chen at BIME NTU
Photograph Example DC Picture
(Before charging) (Charging by PV)
(Fully charged) (Removal of PV)
LC Chen at BIME NTU
0-35 AS
35-70 AS
70-100 AS
Done by TzengFEB 2005
1o Ammonium Sulfate Precipitation
2o Anion Exchange Chromatography
Photograph Example Protein PAGE
LC Chen at BIME NTU
Daytime application an IWECB-
based window can be charged and
colored by a photovoltaic cell
(1) to store solar energy and
(2) to attenuate solar irradiation
Nighttime application an IWECB-
based window can be discharged amp
bleached
(1) to drive low-power devices and
(2) to increase the illumination
Line Drawing Schematic Illustration
LC Chen at BIME NTU
Line Drawing Method and Theory
LC Chen at BIME NTU
Line Drawing Experimental Setup
LC Chen at BIME NTU
01 1 10 100 1000
10x106
10x107
S = SO + S
MAX [A] (K
D + [A]) + k[A]
Ideal model for fitting total bindingK
D = 8 nM k = 7944 K
N ~ O (1 M)
Typ
hoo
n R
eadin
gAptamer No108 Conc (nM)
Surface for TP immobilization
Protein
Aptamer
Biotin
Streptavidin (SA)
Alkaline Phosphatase (AP) Unreacted
Substrate
ReactedSubstrate
Enzyme-Linked Immunosorbent Assay (ELISA)
SA-Biotin interaction Kd ~ 10-15 M = fM ~ ca 260 pNt
Typhoon scan aptamer 5-fold dilution series
Aptamer with nM affinity confirmed
Kd = 8 nM
Combination of Figure Presentation
LC Chen at BIME NTU
Frequently Used Software
bull Word
bull PowerPoint
bull CorelDraw
bull Photoshop
bull Excel
bull Origin
bull Grapher
bull SigmaPlot
bull Othershellip
LC Chen at BIME NTU
LC Chen at BIME NTU
A final and essential step for data presentation consider the efficiency (do things right) and effectiveness (do the right things) Recall the model of ldquoApple Dailyrdquo
If you canrsquot read more papershellip
Can you tell the difference between the figures in a rejected paper and an accepted paper
LC Chen at BIME NTU
同學在論文圖表製作過程中常見違反學術倫理而不自覺的情事
直接剪貼網路( Internet )或文獻( PDF )圖片但未經授權甚至未合理引用 ( cf 原文引用)
製作漂亮數據或使用合成照片以滿足自己的結論
未重複實驗僅報導成功一次的數據
LC Chen at BIME NTU
Essence of Scientific ResearchA valuable thesis is based on the REPRODUCIBLE FACTS AND FIGURES and the most importantly YOUR OWN CREACTIONS NOT NUMBER OF PAGES
httpwwwcartoonstockcomdirectorysscientistasp
LC Chen at BIME NTU
Types of illustrations
bull Chartsgraphs (bar graph frequency histogram XY scatter plot XY line graph)
bull Photographs (including X-rays SEM DNAprotein image)
bull Line drawings (schematic illustration instrumentation)
bull Use to provide evidence emphasis or efficiency
bull As with tables data in charts may be easier to understand than long statements of text
bull Figures with legends and abbreviations should be easily understandable
Figure Prep Rule of Thumb
LC Chen at BIME NTU
bull Determine journalrsquos limitations on number size and types of figuresillustrations
bull Black and white vs colorbull Journal may not accept one or the other
bull Journal may charge for use of color
bull Resolution of images typical requirementsbull At least 300 dpi for photographs
bull 1200 dpi for line art
bull May require glossy prints (hard copies) or specific electronic file types
bull Label all figures according to journalrsquos specifications
bull Clearly mark the correct orientation of the photograph
bull Provide figure legends describing what is shown
Figure Preparation (Contrsquod)
LC Chen at BIME NTU
Anatomy of Figures (Graph)
LC Chen at BIME NTU
Anatomy of Figures (Chart)
LC Chen at BIME NTU
Graph Example Bar Chart
LC Chen at BIME NTU
Graph Example Frequency Histogram
LC Chen at BIME NTU
Graph Example X-Y Scatter Plot
LC Chen at BIME NTU
Graph Example X-Y Line Plot
LC Chen at BIME NTU
ScatterLine Plot Curve Fitting
LC Chen at BIME NTU
Photograph Example SEM
LC Chen at BIME NTU
Photograph Example DC Picture
(Before charging) (Charging by PV)
(Fully charged) (Removal of PV)
LC Chen at BIME NTU
0-35 AS
35-70 AS
70-100 AS
Done by TzengFEB 2005
1o Ammonium Sulfate Precipitation
2o Anion Exchange Chromatography
Photograph Example Protein PAGE
LC Chen at BIME NTU
Daytime application an IWECB-
based window can be charged and
colored by a photovoltaic cell
(1) to store solar energy and
(2) to attenuate solar irradiation
Nighttime application an IWECB-
based window can be discharged amp
bleached
(1) to drive low-power devices and
(2) to increase the illumination
Line Drawing Schematic Illustration
LC Chen at BIME NTU
Line Drawing Method and Theory
LC Chen at BIME NTU
Line Drawing Experimental Setup
LC Chen at BIME NTU
01 1 10 100 1000
10x106
10x107
S = SO + S
MAX [A] (K
D + [A]) + k[A]
Ideal model for fitting total bindingK
D = 8 nM k = 7944 K
N ~ O (1 M)
Typ
hoo
n R
eadin
gAptamer No108 Conc (nM)
Surface for TP immobilization
Protein
Aptamer
Biotin
Streptavidin (SA)
Alkaline Phosphatase (AP) Unreacted
Substrate
ReactedSubstrate
Enzyme-Linked Immunosorbent Assay (ELISA)
SA-Biotin interaction Kd ~ 10-15 M = fM ~ ca 260 pNt
Typhoon scan aptamer 5-fold dilution series
Aptamer with nM affinity confirmed
Kd = 8 nM
Combination of Figure Presentation
LC Chen at BIME NTU
Frequently Used Software
bull Word
bull PowerPoint
bull CorelDraw
bull Photoshop
bull Excel
bull Origin
bull Grapher
bull SigmaPlot
bull Othershellip
LC Chen at BIME NTU
LC Chen at BIME NTU
A final and essential step for data presentation consider the efficiency (do things right) and effectiveness (do the right things) Recall the model of ldquoApple Dailyrdquo
If you canrsquot read more papershellip
Can you tell the difference between the figures in a rejected paper and an accepted paper
LC Chen at BIME NTU
同學在論文圖表製作過程中常見違反學術倫理而不自覺的情事
直接剪貼網路( Internet )或文獻( PDF )圖片但未經授權甚至未合理引用 ( cf 原文引用)
製作漂亮數據或使用合成照片以滿足自己的結論
未重複實驗僅報導成功一次的數據
LC Chen at BIME NTU
Essence of Scientific ResearchA valuable thesis is based on the REPRODUCIBLE FACTS AND FIGURES and the most importantly YOUR OWN CREACTIONS NOT NUMBER OF PAGES
httpwwwcartoonstockcomdirectorysscientistasp
LC Chen at BIME NTU
bull Determine journalrsquos limitations on number size and types of figuresillustrations
bull Black and white vs colorbull Journal may not accept one or the other
bull Journal may charge for use of color
bull Resolution of images typical requirementsbull At least 300 dpi for photographs
bull 1200 dpi for line art
bull May require glossy prints (hard copies) or specific electronic file types
bull Label all figures according to journalrsquos specifications
bull Clearly mark the correct orientation of the photograph
bull Provide figure legends describing what is shown
Figure Preparation (Contrsquod)
LC Chen at BIME NTU
Anatomy of Figures (Graph)
LC Chen at BIME NTU
Anatomy of Figures (Chart)
LC Chen at BIME NTU
Graph Example Bar Chart
LC Chen at BIME NTU
Graph Example Frequency Histogram
LC Chen at BIME NTU
Graph Example X-Y Scatter Plot
LC Chen at BIME NTU
Graph Example X-Y Line Plot
LC Chen at BIME NTU
ScatterLine Plot Curve Fitting
LC Chen at BIME NTU
Photograph Example SEM
LC Chen at BIME NTU
Photograph Example DC Picture
(Before charging) (Charging by PV)
(Fully charged) (Removal of PV)
LC Chen at BIME NTU
0-35 AS
35-70 AS
70-100 AS
Done by TzengFEB 2005
1o Ammonium Sulfate Precipitation
2o Anion Exchange Chromatography
Photograph Example Protein PAGE
LC Chen at BIME NTU
Daytime application an IWECB-
based window can be charged and
colored by a photovoltaic cell
(1) to store solar energy and
(2) to attenuate solar irradiation
Nighttime application an IWECB-
based window can be discharged amp
bleached
(1) to drive low-power devices and
(2) to increase the illumination
Line Drawing Schematic Illustration
LC Chen at BIME NTU
Line Drawing Method and Theory
LC Chen at BIME NTU
Line Drawing Experimental Setup
LC Chen at BIME NTU
01 1 10 100 1000
10x106
10x107
S = SO + S
MAX [A] (K
D + [A]) + k[A]
Ideal model for fitting total bindingK
D = 8 nM k = 7944 K
N ~ O (1 M)
Typ
hoo
n R
eadin
gAptamer No108 Conc (nM)
Surface for TP immobilization
Protein
Aptamer
Biotin
Streptavidin (SA)
Alkaline Phosphatase (AP) Unreacted
Substrate
ReactedSubstrate
Enzyme-Linked Immunosorbent Assay (ELISA)
SA-Biotin interaction Kd ~ 10-15 M = fM ~ ca 260 pNt
Typhoon scan aptamer 5-fold dilution series
Aptamer with nM affinity confirmed
Kd = 8 nM
Combination of Figure Presentation
LC Chen at BIME NTU
Frequently Used Software
bull Word
bull PowerPoint
bull CorelDraw
bull Photoshop
bull Excel
bull Origin
bull Grapher
bull SigmaPlot
bull Othershellip
LC Chen at BIME NTU
LC Chen at BIME NTU
A final and essential step for data presentation consider the efficiency (do things right) and effectiveness (do the right things) Recall the model of ldquoApple Dailyrdquo
If you canrsquot read more papershellip
Can you tell the difference between the figures in a rejected paper and an accepted paper
LC Chen at BIME NTU
同學在論文圖表製作過程中常見違反學術倫理而不自覺的情事
直接剪貼網路( Internet )或文獻( PDF )圖片但未經授權甚至未合理引用 ( cf 原文引用)
製作漂亮數據或使用合成照片以滿足自己的結論
未重複實驗僅報導成功一次的數據
LC Chen at BIME NTU
Essence of Scientific ResearchA valuable thesis is based on the REPRODUCIBLE FACTS AND FIGURES and the most importantly YOUR OWN CREACTIONS NOT NUMBER OF PAGES
httpwwwcartoonstockcomdirectorysscientistasp
LC Chen at BIME NTU
Anatomy of Figures (Graph)
LC Chen at BIME NTU
Anatomy of Figures (Chart)
LC Chen at BIME NTU
Graph Example Bar Chart
LC Chen at BIME NTU
Graph Example Frequency Histogram
LC Chen at BIME NTU
Graph Example X-Y Scatter Plot
LC Chen at BIME NTU
Graph Example X-Y Line Plot
LC Chen at BIME NTU
ScatterLine Plot Curve Fitting
LC Chen at BIME NTU
Photograph Example SEM
LC Chen at BIME NTU
Photograph Example DC Picture
(Before charging) (Charging by PV)
(Fully charged) (Removal of PV)
LC Chen at BIME NTU
0-35 AS
35-70 AS
70-100 AS
Done by TzengFEB 2005
1o Ammonium Sulfate Precipitation
2o Anion Exchange Chromatography
Photograph Example Protein PAGE
LC Chen at BIME NTU
Daytime application an IWECB-
based window can be charged and
colored by a photovoltaic cell
(1) to store solar energy and
(2) to attenuate solar irradiation
Nighttime application an IWECB-
based window can be discharged amp
bleached
(1) to drive low-power devices and
(2) to increase the illumination
Line Drawing Schematic Illustration
LC Chen at BIME NTU
Line Drawing Method and Theory
LC Chen at BIME NTU
Line Drawing Experimental Setup
LC Chen at BIME NTU
01 1 10 100 1000
10x106
10x107
S = SO + S
MAX [A] (K
D + [A]) + k[A]
Ideal model for fitting total bindingK
D = 8 nM k = 7944 K
N ~ O (1 M)
Typ
hoo
n R
eadin
gAptamer No108 Conc (nM)
Surface for TP immobilization
Protein
Aptamer
Biotin
Streptavidin (SA)
Alkaline Phosphatase (AP) Unreacted
Substrate
ReactedSubstrate
Enzyme-Linked Immunosorbent Assay (ELISA)
SA-Biotin interaction Kd ~ 10-15 M = fM ~ ca 260 pNt
Typhoon scan aptamer 5-fold dilution series
Aptamer with nM affinity confirmed
Kd = 8 nM
Combination of Figure Presentation
LC Chen at BIME NTU
Frequently Used Software
bull Word
bull PowerPoint
bull CorelDraw
bull Photoshop
bull Excel
bull Origin
bull Grapher
bull SigmaPlot
bull Othershellip
LC Chen at BIME NTU
LC Chen at BIME NTU
A final and essential step for data presentation consider the efficiency (do things right) and effectiveness (do the right things) Recall the model of ldquoApple Dailyrdquo
If you canrsquot read more papershellip
Can you tell the difference between the figures in a rejected paper and an accepted paper
LC Chen at BIME NTU
同學在論文圖表製作過程中常見違反學術倫理而不自覺的情事
直接剪貼網路( Internet )或文獻( PDF )圖片但未經授權甚至未合理引用 ( cf 原文引用)
製作漂亮數據或使用合成照片以滿足自己的結論
未重複實驗僅報導成功一次的數據
LC Chen at BIME NTU
Essence of Scientific ResearchA valuable thesis is based on the REPRODUCIBLE FACTS AND FIGURES and the most importantly YOUR OWN CREACTIONS NOT NUMBER OF PAGES
httpwwwcartoonstockcomdirectorysscientistasp
LC Chen at BIME NTU
Anatomy of Figures (Chart)
LC Chen at BIME NTU
Graph Example Bar Chart
LC Chen at BIME NTU
Graph Example Frequency Histogram
LC Chen at BIME NTU
Graph Example X-Y Scatter Plot
LC Chen at BIME NTU
Graph Example X-Y Line Plot
LC Chen at BIME NTU
ScatterLine Plot Curve Fitting
LC Chen at BIME NTU
Photograph Example SEM
LC Chen at BIME NTU
Photograph Example DC Picture
(Before charging) (Charging by PV)
(Fully charged) (Removal of PV)
LC Chen at BIME NTU
0-35 AS
35-70 AS
70-100 AS
Done by TzengFEB 2005
1o Ammonium Sulfate Precipitation
2o Anion Exchange Chromatography
Photograph Example Protein PAGE
LC Chen at BIME NTU
Daytime application an IWECB-
based window can be charged and
colored by a photovoltaic cell
(1) to store solar energy and
(2) to attenuate solar irradiation
Nighttime application an IWECB-
based window can be discharged amp
bleached
(1) to drive low-power devices and
(2) to increase the illumination
Line Drawing Schematic Illustration
LC Chen at BIME NTU
Line Drawing Method and Theory
LC Chen at BIME NTU
Line Drawing Experimental Setup
LC Chen at BIME NTU
01 1 10 100 1000
10x106
10x107
S = SO + S
MAX [A] (K
D + [A]) + k[A]
Ideal model for fitting total bindingK
D = 8 nM k = 7944 K
N ~ O (1 M)
Typ
hoo
n R
eadin
gAptamer No108 Conc (nM)
Surface for TP immobilization
Protein
Aptamer
Biotin
Streptavidin (SA)
Alkaline Phosphatase (AP) Unreacted
Substrate
ReactedSubstrate
Enzyme-Linked Immunosorbent Assay (ELISA)
SA-Biotin interaction Kd ~ 10-15 M = fM ~ ca 260 pNt
Typhoon scan aptamer 5-fold dilution series
Aptamer with nM affinity confirmed
Kd = 8 nM
Combination of Figure Presentation
LC Chen at BIME NTU
Frequently Used Software
bull Word
bull PowerPoint
bull CorelDraw
bull Photoshop
bull Excel
bull Origin
bull Grapher
bull SigmaPlot
bull Othershellip
LC Chen at BIME NTU
LC Chen at BIME NTU
A final and essential step for data presentation consider the efficiency (do things right) and effectiveness (do the right things) Recall the model of ldquoApple Dailyrdquo
If you canrsquot read more papershellip
Can you tell the difference between the figures in a rejected paper and an accepted paper
LC Chen at BIME NTU
同學在論文圖表製作過程中常見違反學術倫理而不自覺的情事
直接剪貼網路( Internet )或文獻( PDF )圖片但未經授權甚至未合理引用 ( cf 原文引用)
製作漂亮數據或使用合成照片以滿足自己的結論
未重複實驗僅報導成功一次的數據
LC Chen at BIME NTU
Essence of Scientific ResearchA valuable thesis is based on the REPRODUCIBLE FACTS AND FIGURES and the most importantly YOUR OWN CREACTIONS NOT NUMBER OF PAGES
httpwwwcartoonstockcomdirectorysscientistasp
LC Chen at BIME NTU
Graph Example Bar Chart
LC Chen at BIME NTU
Graph Example Frequency Histogram
LC Chen at BIME NTU
Graph Example X-Y Scatter Plot
LC Chen at BIME NTU
Graph Example X-Y Line Plot
LC Chen at BIME NTU
ScatterLine Plot Curve Fitting
LC Chen at BIME NTU
Photograph Example SEM
LC Chen at BIME NTU
Photograph Example DC Picture
(Before charging) (Charging by PV)
(Fully charged) (Removal of PV)
LC Chen at BIME NTU
0-35 AS
35-70 AS
70-100 AS
Done by TzengFEB 2005
1o Ammonium Sulfate Precipitation
2o Anion Exchange Chromatography
Photograph Example Protein PAGE
LC Chen at BIME NTU
Daytime application an IWECB-
based window can be charged and
colored by a photovoltaic cell
(1) to store solar energy and
(2) to attenuate solar irradiation
Nighttime application an IWECB-
based window can be discharged amp
bleached
(1) to drive low-power devices and
(2) to increase the illumination
Line Drawing Schematic Illustration
LC Chen at BIME NTU
Line Drawing Method and Theory
LC Chen at BIME NTU
Line Drawing Experimental Setup
LC Chen at BIME NTU
01 1 10 100 1000
10x106
10x107
S = SO + S
MAX [A] (K
D + [A]) + k[A]
Ideal model for fitting total bindingK
D = 8 nM k = 7944 K
N ~ O (1 M)
Typ
hoo
n R
eadin
gAptamer No108 Conc (nM)
Surface for TP immobilization
Protein
Aptamer
Biotin
Streptavidin (SA)
Alkaline Phosphatase (AP) Unreacted
Substrate
ReactedSubstrate
Enzyme-Linked Immunosorbent Assay (ELISA)
SA-Biotin interaction Kd ~ 10-15 M = fM ~ ca 260 pNt
Typhoon scan aptamer 5-fold dilution series
Aptamer with nM affinity confirmed
Kd = 8 nM
Combination of Figure Presentation
LC Chen at BIME NTU
Frequently Used Software
bull Word
bull PowerPoint
bull CorelDraw
bull Photoshop
bull Excel
bull Origin
bull Grapher
bull SigmaPlot
bull Othershellip
LC Chen at BIME NTU
LC Chen at BIME NTU
A final and essential step for data presentation consider the efficiency (do things right) and effectiveness (do the right things) Recall the model of ldquoApple Dailyrdquo
If you canrsquot read more papershellip
Can you tell the difference between the figures in a rejected paper and an accepted paper
LC Chen at BIME NTU
同學在論文圖表製作過程中常見違反學術倫理而不自覺的情事
直接剪貼網路( Internet )或文獻( PDF )圖片但未經授權甚至未合理引用 ( cf 原文引用)
製作漂亮數據或使用合成照片以滿足自己的結論
未重複實驗僅報導成功一次的數據
LC Chen at BIME NTU
Essence of Scientific ResearchA valuable thesis is based on the REPRODUCIBLE FACTS AND FIGURES and the most importantly YOUR OWN CREACTIONS NOT NUMBER OF PAGES
httpwwwcartoonstockcomdirectorysscientistasp
LC Chen at BIME NTU
Graph Example Frequency Histogram
LC Chen at BIME NTU
Graph Example X-Y Scatter Plot
LC Chen at BIME NTU
Graph Example X-Y Line Plot
LC Chen at BIME NTU
ScatterLine Plot Curve Fitting
LC Chen at BIME NTU
Photograph Example SEM
LC Chen at BIME NTU
Photograph Example DC Picture
(Before charging) (Charging by PV)
(Fully charged) (Removal of PV)
LC Chen at BIME NTU
0-35 AS
35-70 AS
70-100 AS
Done by TzengFEB 2005
1o Ammonium Sulfate Precipitation
2o Anion Exchange Chromatography
Photograph Example Protein PAGE
LC Chen at BIME NTU
Daytime application an IWECB-
based window can be charged and
colored by a photovoltaic cell
(1) to store solar energy and
(2) to attenuate solar irradiation
Nighttime application an IWECB-
based window can be discharged amp
bleached
(1) to drive low-power devices and
(2) to increase the illumination
Line Drawing Schematic Illustration
LC Chen at BIME NTU
Line Drawing Method and Theory
LC Chen at BIME NTU
Line Drawing Experimental Setup
LC Chen at BIME NTU
01 1 10 100 1000
10x106
10x107
S = SO + S
MAX [A] (K
D + [A]) + k[A]
Ideal model for fitting total bindingK
D = 8 nM k = 7944 K
N ~ O (1 M)
Typ
hoo
n R
eadin
gAptamer No108 Conc (nM)
Surface for TP immobilization
Protein
Aptamer
Biotin
Streptavidin (SA)
Alkaline Phosphatase (AP) Unreacted
Substrate
ReactedSubstrate
Enzyme-Linked Immunosorbent Assay (ELISA)
SA-Biotin interaction Kd ~ 10-15 M = fM ~ ca 260 pNt
Typhoon scan aptamer 5-fold dilution series
Aptamer with nM affinity confirmed
Kd = 8 nM
Combination of Figure Presentation
LC Chen at BIME NTU
Frequently Used Software
bull Word
bull PowerPoint
bull CorelDraw
bull Photoshop
bull Excel
bull Origin
bull Grapher
bull SigmaPlot
bull Othershellip
LC Chen at BIME NTU
LC Chen at BIME NTU
A final and essential step for data presentation consider the efficiency (do things right) and effectiveness (do the right things) Recall the model of ldquoApple Dailyrdquo
If you canrsquot read more papershellip
Can you tell the difference between the figures in a rejected paper and an accepted paper
LC Chen at BIME NTU
同學在論文圖表製作過程中常見違反學術倫理而不自覺的情事
直接剪貼網路( Internet )或文獻( PDF )圖片但未經授權甚至未合理引用 ( cf 原文引用)
製作漂亮數據或使用合成照片以滿足自己的結論
未重複實驗僅報導成功一次的數據
LC Chen at BIME NTU
Essence of Scientific ResearchA valuable thesis is based on the REPRODUCIBLE FACTS AND FIGURES and the most importantly YOUR OWN CREACTIONS NOT NUMBER OF PAGES
httpwwwcartoonstockcomdirectorysscientistasp
LC Chen at BIME NTU
Graph Example X-Y Scatter Plot
LC Chen at BIME NTU
Graph Example X-Y Line Plot
LC Chen at BIME NTU
ScatterLine Plot Curve Fitting
LC Chen at BIME NTU
Photograph Example SEM
LC Chen at BIME NTU
Photograph Example DC Picture
(Before charging) (Charging by PV)
(Fully charged) (Removal of PV)
LC Chen at BIME NTU
0-35 AS
35-70 AS
70-100 AS
Done by TzengFEB 2005
1o Ammonium Sulfate Precipitation
2o Anion Exchange Chromatography
Photograph Example Protein PAGE
LC Chen at BIME NTU
Daytime application an IWECB-
based window can be charged and
colored by a photovoltaic cell
(1) to store solar energy and
(2) to attenuate solar irradiation
Nighttime application an IWECB-
based window can be discharged amp
bleached
(1) to drive low-power devices and
(2) to increase the illumination
Line Drawing Schematic Illustration
LC Chen at BIME NTU
Line Drawing Method and Theory
LC Chen at BIME NTU
Line Drawing Experimental Setup
LC Chen at BIME NTU
01 1 10 100 1000
10x106
10x107
S = SO + S
MAX [A] (K
D + [A]) + k[A]
Ideal model for fitting total bindingK
D = 8 nM k = 7944 K
N ~ O (1 M)
Typ
hoo
n R
eadin
gAptamer No108 Conc (nM)
Surface for TP immobilization
Protein
Aptamer
Biotin
Streptavidin (SA)
Alkaline Phosphatase (AP) Unreacted
Substrate
ReactedSubstrate
Enzyme-Linked Immunosorbent Assay (ELISA)
SA-Biotin interaction Kd ~ 10-15 M = fM ~ ca 260 pNt
Typhoon scan aptamer 5-fold dilution series
Aptamer with nM affinity confirmed
Kd = 8 nM
Combination of Figure Presentation
LC Chen at BIME NTU
Frequently Used Software
bull Word
bull PowerPoint
bull CorelDraw
bull Photoshop
bull Excel
bull Origin
bull Grapher
bull SigmaPlot
bull Othershellip
LC Chen at BIME NTU
LC Chen at BIME NTU
A final and essential step for data presentation consider the efficiency (do things right) and effectiveness (do the right things) Recall the model of ldquoApple Dailyrdquo
If you canrsquot read more papershellip
Can you tell the difference between the figures in a rejected paper and an accepted paper
LC Chen at BIME NTU
同學在論文圖表製作過程中常見違反學術倫理而不自覺的情事
直接剪貼網路( Internet )或文獻( PDF )圖片但未經授權甚至未合理引用 ( cf 原文引用)
製作漂亮數據或使用合成照片以滿足自己的結論
未重複實驗僅報導成功一次的數據
LC Chen at BIME NTU
Essence of Scientific ResearchA valuable thesis is based on the REPRODUCIBLE FACTS AND FIGURES and the most importantly YOUR OWN CREACTIONS NOT NUMBER OF PAGES
httpwwwcartoonstockcomdirectorysscientistasp
LC Chen at BIME NTU
Graph Example X-Y Line Plot
LC Chen at BIME NTU
ScatterLine Plot Curve Fitting
LC Chen at BIME NTU
Photograph Example SEM
LC Chen at BIME NTU
Photograph Example DC Picture
(Before charging) (Charging by PV)
(Fully charged) (Removal of PV)
LC Chen at BIME NTU
0-35 AS
35-70 AS
70-100 AS
Done by TzengFEB 2005
1o Ammonium Sulfate Precipitation
2o Anion Exchange Chromatography
Photograph Example Protein PAGE
LC Chen at BIME NTU
Daytime application an IWECB-
based window can be charged and
colored by a photovoltaic cell
(1) to store solar energy and
(2) to attenuate solar irradiation
Nighttime application an IWECB-
based window can be discharged amp
bleached
(1) to drive low-power devices and
(2) to increase the illumination
Line Drawing Schematic Illustration
LC Chen at BIME NTU
Line Drawing Method and Theory
LC Chen at BIME NTU
Line Drawing Experimental Setup
LC Chen at BIME NTU
01 1 10 100 1000
10x106
10x107
S = SO + S
MAX [A] (K
D + [A]) + k[A]
Ideal model for fitting total bindingK
D = 8 nM k = 7944 K
N ~ O (1 M)
Typ
hoo
n R
eadin
gAptamer No108 Conc (nM)
Surface for TP immobilization
Protein
Aptamer
Biotin
Streptavidin (SA)
Alkaline Phosphatase (AP) Unreacted
Substrate
ReactedSubstrate
Enzyme-Linked Immunosorbent Assay (ELISA)
SA-Biotin interaction Kd ~ 10-15 M = fM ~ ca 260 pNt
Typhoon scan aptamer 5-fold dilution series
Aptamer with nM affinity confirmed
Kd = 8 nM
Combination of Figure Presentation
LC Chen at BIME NTU
Frequently Used Software
bull Word
bull PowerPoint
bull CorelDraw
bull Photoshop
bull Excel
bull Origin
bull Grapher
bull SigmaPlot
bull Othershellip
LC Chen at BIME NTU
LC Chen at BIME NTU
A final and essential step for data presentation consider the efficiency (do things right) and effectiveness (do the right things) Recall the model of ldquoApple Dailyrdquo
If you canrsquot read more papershellip
Can you tell the difference between the figures in a rejected paper and an accepted paper
LC Chen at BIME NTU
同學在論文圖表製作過程中常見違反學術倫理而不自覺的情事
直接剪貼網路( Internet )或文獻( PDF )圖片但未經授權甚至未合理引用 ( cf 原文引用)
製作漂亮數據或使用合成照片以滿足自己的結論
未重複實驗僅報導成功一次的數據
LC Chen at BIME NTU
Essence of Scientific ResearchA valuable thesis is based on the REPRODUCIBLE FACTS AND FIGURES and the most importantly YOUR OWN CREACTIONS NOT NUMBER OF PAGES
httpwwwcartoonstockcomdirectorysscientistasp
LC Chen at BIME NTU
ScatterLine Plot Curve Fitting
LC Chen at BIME NTU
Photograph Example SEM
LC Chen at BIME NTU
Photograph Example DC Picture
(Before charging) (Charging by PV)
(Fully charged) (Removal of PV)
LC Chen at BIME NTU
0-35 AS
35-70 AS
70-100 AS
Done by TzengFEB 2005
1o Ammonium Sulfate Precipitation
2o Anion Exchange Chromatography
Photograph Example Protein PAGE
LC Chen at BIME NTU
Daytime application an IWECB-
based window can be charged and
colored by a photovoltaic cell
(1) to store solar energy and
(2) to attenuate solar irradiation
Nighttime application an IWECB-
based window can be discharged amp
bleached
(1) to drive low-power devices and
(2) to increase the illumination
Line Drawing Schematic Illustration
LC Chen at BIME NTU
Line Drawing Method and Theory
LC Chen at BIME NTU
Line Drawing Experimental Setup
LC Chen at BIME NTU
01 1 10 100 1000
10x106
10x107
S = SO + S
MAX [A] (K
D + [A]) + k[A]
Ideal model for fitting total bindingK
D = 8 nM k = 7944 K
N ~ O (1 M)
Typ
hoo
n R
eadin
gAptamer No108 Conc (nM)
Surface for TP immobilization
Protein
Aptamer
Biotin
Streptavidin (SA)
Alkaline Phosphatase (AP) Unreacted
Substrate
ReactedSubstrate
Enzyme-Linked Immunosorbent Assay (ELISA)
SA-Biotin interaction Kd ~ 10-15 M = fM ~ ca 260 pNt
Typhoon scan aptamer 5-fold dilution series
Aptamer with nM affinity confirmed
Kd = 8 nM
Combination of Figure Presentation
LC Chen at BIME NTU
Frequently Used Software
bull Word
bull PowerPoint
bull CorelDraw
bull Photoshop
bull Excel
bull Origin
bull Grapher
bull SigmaPlot
bull Othershellip
LC Chen at BIME NTU
LC Chen at BIME NTU
A final and essential step for data presentation consider the efficiency (do things right) and effectiveness (do the right things) Recall the model of ldquoApple Dailyrdquo
If you canrsquot read more papershellip
Can you tell the difference between the figures in a rejected paper and an accepted paper
LC Chen at BIME NTU
同學在論文圖表製作過程中常見違反學術倫理而不自覺的情事
直接剪貼網路( Internet )或文獻( PDF )圖片但未經授權甚至未合理引用 ( cf 原文引用)
製作漂亮數據或使用合成照片以滿足自己的結論
未重複實驗僅報導成功一次的數據
LC Chen at BIME NTU
Essence of Scientific ResearchA valuable thesis is based on the REPRODUCIBLE FACTS AND FIGURES and the most importantly YOUR OWN CREACTIONS NOT NUMBER OF PAGES
httpwwwcartoonstockcomdirectorysscientistasp
LC Chen at BIME NTU
Photograph Example SEM
LC Chen at BIME NTU
Photograph Example DC Picture
(Before charging) (Charging by PV)
(Fully charged) (Removal of PV)
LC Chen at BIME NTU
0-35 AS
35-70 AS
70-100 AS
Done by TzengFEB 2005
1o Ammonium Sulfate Precipitation
2o Anion Exchange Chromatography
Photograph Example Protein PAGE
LC Chen at BIME NTU
Daytime application an IWECB-
based window can be charged and
colored by a photovoltaic cell
(1) to store solar energy and
(2) to attenuate solar irradiation
Nighttime application an IWECB-
based window can be discharged amp
bleached
(1) to drive low-power devices and
(2) to increase the illumination
Line Drawing Schematic Illustration
LC Chen at BIME NTU
Line Drawing Method and Theory
LC Chen at BIME NTU
Line Drawing Experimental Setup
LC Chen at BIME NTU
01 1 10 100 1000
10x106
10x107
S = SO + S
MAX [A] (K
D + [A]) + k[A]
Ideal model for fitting total bindingK
D = 8 nM k = 7944 K
N ~ O (1 M)
Typ
hoo
n R
eadin
gAptamer No108 Conc (nM)
Surface for TP immobilization
Protein
Aptamer
Biotin
Streptavidin (SA)
Alkaline Phosphatase (AP) Unreacted
Substrate
ReactedSubstrate
Enzyme-Linked Immunosorbent Assay (ELISA)
SA-Biotin interaction Kd ~ 10-15 M = fM ~ ca 260 pNt
Typhoon scan aptamer 5-fold dilution series
Aptamer with nM affinity confirmed
Kd = 8 nM
Combination of Figure Presentation
LC Chen at BIME NTU
Frequently Used Software
bull Word
bull PowerPoint
bull CorelDraw
bull Photoshop
bull Excel
bull Origin
bull Grapher
bull SigmaPlot
bull Othershellip
LC Chen at BIME NTU
LC Chen at BIME NTU
A final and essential step for data presentation consider the efficiency (do things right) and effectiveness (do the right things) Recall the model of ldquoApple Dailyrdquo
If you canrsquot read more papershellip
Can you tell the difference between the figures in a rejected paper and an accepted paper
LC Chen at BIME NTU
同學在論文圖表製作過程中常見違反學術倫理而不自覺的情事
直接剪貼網路( Internet )或文獻( PDF )圖片但未經授權甚至未合理引用 ( cf 原文引用)
製作漂亮數據或使用合成照片以滿足自己的結論
未重複實驗僅報導成功一次的數據
LC Chen at BIME NTU
Essence of Scientific ResearchA valuable thesis is based on the REPRODUCIBLE FACTS AND FIGURES and the most importantly YOUR OWN CREACTIONS NOT NUMBER OF PAGES
httpwwwcartoonstockcomdirectorysscientistasp
LC Chen at BIME NTU
Photograph Example DC Picture
(Before charging) (Charging by PV)
(Fully charged) (Removal of PV)
LC Chen at BIME NTU
0-35 AS
35-70 AS
70-100 AS
Done by TzengFEB 2005
1o Ammonium Sulfate Precipitation
2o Anion Exchange Chromatography
Photograph Example Protein PAGE
LC Chen at BIME NTU
Daytime application an IWECB-
based window can be charged and
colored by a photovoltaic cell
(1) to store solar energy and
(2) to attenuate solar irradiation
Nighttime application an IWECB-
based window can be discharged amp
bleached
(1) to drive low-power devices and
(2) to increase the illumination
Line Drawing Schematic Illustration
LC Chen at BIME NTU
Line Drawing Method and Theory
LC Chen at BIME NTU
Line Drawing Experimental Setup
LC Chen at BIME NTU
01 1 10 100 1000
10x106
10x107
S = SO + S
MAX [A] (K
D + [A]) + k[A]
Ideal model for fitting total bindingK
D = 8 nM k = 7944 K
N ~ O (1 M)
Typ
hoo
n R
eadin
gAptamer No108 Conc (nM)
Surface for TP immobilization
Protein
Aptamer
Biotin
Streptavidin (SA)
Alkaline Phosphatase (AP) Unreacted
Substrate
ReactedSubstrate
Enzyme-Linked Immunosorbent Assay (ELISA)
SA-Biotin interaction Kd ~ 10-15 M = fM ~ ca 260 pNt
Typhoon scan aptamer 5-fold dilution series
Aptamer with nM affinity confirmed
Kd = 8 nM
Combination of Figure Presentation
LC Chen at BIME NTU
Frequently Used Software
bull Word
bull PowerPoint
bull CorelDraw
bull Photoshop
bull Excel
bull Origin
bull Grapher
bull SigmaPlot
bull Othershellip
LC Chen at BIME NTU
LC Chen at BIME NTU
A final and essential step for data presentation consider the efficiency (do things right) and effectiveness (do the right things) Recall the model of ldquoApple Dailyrdquo
If you canrsquot read more papershellip
Can you tell the difference between the figures in a rejected paper and an accepted paper
LC Chen at BIME NTU
同學在論文圖表製作過程中常見違反學術倫理而不自覺的情事
直接剪貼網路( Internet )或文獻( PDF )圖片但未經授權甚至未合理引用 ( cf 原文引用)
製作漂亮數據或使用合成照片以滿足自己的結論
未重複實驗僅報導成功一次的數據
LC Chen at BIME NTU
Essence of Scientific ResearchA valuable thesis is based on the REPRODUCIBLE FACTS AND FIGURES and the most importantly YOUR OWN CREACTIONS NOT NUMBER OF PAGES
httpwwwcartoonstockcomdirectorysscientistasp
LC Chen at BIME NTU
0-35 AS
35-70 AS
70-100 AS
Done by TzengFEB 2005
1o Ammonium Sulfate Precipitation
2o Anion Exchange Chromatography
Photograph Example Protein PAGE
LC Chen at BIME NTU
Daytime application an IWECB-
based window can be charged and
colored by a photovoltaic cell
(1) to store solar energy and
(2) to attenuate solar irradiation
Nighttime application an IWECB-
based window can be discharged amp
bleached
(1) to drive low-power devices and
(2) to increase the illumination
Line Drawing Schematic Illustration
LC Chen at BIME NTU
Line Drawing Method and Theory
LC Chen at BIME NTU
Line Drawing Experimental Setup
LC Chen at BIME NTU
01 1 10 100 1000
10x106
10x107
S = SO + S
MAX [A] (K
D + [A]) + k[A]
Ideal model for fitting total bindingK
D = 8 nM k = 7944 K
N ~ O (1 M)
Typ
hoo
n R
eadin
gAptamer No108 Conc (nM)
Surface for TP immobilization
Protein
Aptamer
Biotin
Streptavidin (SA)
Alkaline Phosphatase (AP) Unreacted
Substrate
ReactedSubstrate
Enzyme-Linked Immunosorbent Assay (ELISA)
SA-Biotin interaction Kd ~ 10-15 M = fM ~ ca 260 pNt
Typhoon scan aptamer 5-fold dilution series
Aptamer with nM affinity confirmed
Kd = 8 nM
Combination of Figure Presentation
LC Chen at BIME NTU
Frequently Used Software
bull Word
bull PowerPoint
bull CorelDraw
bull Photoshop
bull Excel
bull Origin
bull Grapher
bull SigmaPlot
bull Othershellip
LC Chen at BIME NTU
LC Chen at BIME NTU
A final and essential step for data presentation consider the efficiency (do things right) and effectiveness (do the right things) Recall the model of ldquoApple Dailyrdquo
If you canrsquot read more papershellip
Can you tell the difference between the figures in a rejected paper and an accepted paper
LC Chen at BIME NTU
同學在論文圖表製作過程中常見違反學術倫理而不自覺的情事
直接剪貼網路( Internet )或文獻( PDF )圖片但未經授權甚至未合理引用 ( cf 原文引用)
製作漂亮數據或使用合成照片以滿足自己的結論
未重複實驗僅報導成功一次的數據
LC Chen at BIME NTU
Essence of Scientific ResearchA valuable thesis is based on the REPRODUCIBLE FACTS AND FIGURES and the most importantly YOUR OWN CREACTIONS NOT NUMBER OF PAGES
httpwwwcartoonstockcomdirectorysscientistasp
LC Chen at BIME NTU
Daytime application an IWECB-
based window can be charged and
colored by a photovoltaic cell
(1) to store solar energy and
(2) to attenuate solar irradiation
Nighttime application an IWECB-
based window can be discharged amp
bleached
(1) to drive low-power devices and
(2) to increase the illumination
Line Drawing Schematic Illustration
LC Chen at BIME NTU
Line Drawing Method and Theory
LC Chen at BIME NTU
Line Drawing Experimental Setup
LC Chen at BIME NTU
01 1 10 100 1000
10x106
10x107
S = SO + S
MAX [A] (K
D + [A]) + k[A]
Ideal model for fitting total bindingK
D = 8 nM k = 7944 K
N ~ O (1 M)
Typ
hoo
n R
eadin
gAptamer No108 Conc (nM)
Surface for TP immobilization
Protein
Aptamer
Biotin
Streptavidin (SA)
Alkaline Phosphatase (AP) Unreacted
Substrate
ReactedSubstrate
Enzyme-Linked Immunosorbent Assay (ELISA)
SA-Biotin interaction Kd ~ 10-15 M = fM ~ ca 260 pNt
Typhoon scan aptamer 5-fold dilution series
Aptamer with nM affinity confirmed
Kd = 8 nM
Combination of Figure Presentation
LC Chen at BIME NTU
Frequently Used Software
bull Word
bull PowerPoint
bull CorelDraw
bull Photoshop
bull Excel
bull Origin
bull Grapher
bull SigmaPlot
bull Othershellip
LC Chen at BIME NTU
LC Chen at BIME NTU
A final and essential step for data presentation consider the efficiency (do things right) and effectiveness (do the right things) Recall the model of ldquoApple Dailyrdquo
If you canrsquot read more papershellip
Can you tell the difference between the figures in a rejected paper and an accepted paper
LC Chen at BIME NTU
同學在論文圖表製作過程中常見違反學術倫理而不自覺的情事
直接剪貼網路( Internet )或文獻( PDF )圖片但未經授權甚至未合理引用 ( cf 原文引用)
製作漂亮數據或使用合成照片以滿足自己的結論
未重複實驗僅報導成功一次的數據
LC Chen at BIME NTU
Essence of Scientific ResearchA valuable thesis is based on the REPRODUCIBLE FACTS AND FIGURES and the most importantly YOUR OWN CREACTIONS NOT NUMBER OF PAGES
httpwwwcartoonstockcomdirectorysscientistasp
LC Chen at BIME NTU
Line Drawing Method and Theory
LC Chen at BIME NTU
Line Drawing Experimental Setup
LC Chen at BIME NTU
01 1 10 100 1000
10x106
10x107
S = SO + S
MAX [A] (K
D + [A]) + k[A]
Ideal model for fitting total bindingK
D = 8 nM k = 7944 K
N ~ O (1 M)
Typ
hoo
n R
eadin
gAptamer No108 Conc (nM)
Surface for TP immobilization
Protein
Aptamer
Biotin
Streptavidin (SA)
Alkaline Phosphatase (AP) Unreacted
Substrate
ReactedSubstrate
Enzyme-Linked Immunosorbent Assay (ELISA)
SA-Biotin interaction Kd ~ 10-15 M = fM ~ ca 260 pNt
Typhoon scan aptamer 5-fold dilution series
Aptamer with nM affinity confirmed
Kd = 8 nM
Combination of Figure Presentation
LC Chen at BIME NTU
Frequently Used Software
bull Word
bull PowerPoint
bull CorelDraw
bull Photoshop
bull Excel
bull Origin
bull Grapher
bull SigmaPlot
bull Othershellip
LC Chen at BIME NTU
LC Chen at BIME NTU
A final and essential step for data presentation consider the efficiency (do things right) and effectiveness (do the right things) Recall the model of ldquoApple Dailyrdquo
If you canrsquot read more papershellip
Can you tell the difference between the figures in a rejected paper and an accepted paper
LC Chen at BIME NTU
同學在論文圖表製作過程中常見違反學術倫理而不自覺的情事
直接剪貼網路( Internet )或文獻( PDF )圖片但未經授權甚至未合理引用 ( cf 原文引用)
製作漂亮數據或使用合成照片以滿足自己的結論
未重複實驗僅報導成功一次的數據
LC Chen at BIME NTU
Essence of Scientific ResearchA valuable thesis is based on the REPRODUCIBLE FACTS AND FIGURES and the most importantly YOUR OWN CREACTIONS NOT NUMBER OF PAGES
httpwwwcartoonstockcomdirectorysscientistasp
LC Chen at BIME NTU
Line Drawing Experimental Setup
LC Chen at BIME NTU
01 1 10 100 1000
10x106
10x107
S = SO + S
MAX [A] (K
D + [A]) + k[A]
Ideal model for fitting total bindingK
D = 8 nM k = 7944 K
N ~ O (1 M)
Typ
hoo
n R
eadin
gAptamer No108 Conc (nM)
Surface for TP immobilization
Protein
Aptamer
Biotin
Streptavidin (SA)
Alkaline Phosphatase (AP) Unreacted
Substrate
ReactedSubstrate
Enzyme-Linked Immunosorbent Assay (ELISA)
SA-Biotin interaction Kd ~ 10-15 M = fM ~ ca 260 pNt
Typhoon scan aptamer 5-fold dilution series
Aptamer with nM affinity confirmed
Kd = 8 nM
Combination of Figure Presentation
LC Chen at BIME NTU
Frequently Used Software
bull Word
bull PowerPoint
bull CorelDraw
bull Photoshop
bull Excel
bull Origin
bull Grapher
bull SigmaPlot
bull Othershellip
LC Chen at BIME NTU
LC Chen at BIME NTU
A final and essential step for data presentation consider the efficiency (do things right) and effectiveness (do the right things) Recall the model of ldquoApple Dailyrdquo
If you canrsquot read more papershellip
Can you tell the difference between the figures in a rejected paper and an accepted paper
LC Chen at BIME NTU
同學在論文圖表製作過程中常見違反學術倫理而不自覺的情事
直接剪貼網路( Internet )或文獻( PDF )圖片但未經授權甚至未合理引用 ( cf 原文引用)
製作漂亮數據或使用合成照片以滿足自己的結論
未重複實驗僅報導成功一次的數據
LC Chen at BIME NTU
Essence of Scientific ResearchA valuable thesis is based on the REPRODUCIBLE FACTS AND FIGURES and the most importantly YOUR OWN CREACTIONS NOT NUMBER OF PAGES
httpwwwcartoonstockcomdirectorysscientistasp
LC Chen at BIME NTU
01 1 10 100 1000
10x106
10x107
S = SO + S
MAX [A] (K
D + [A]) + k[A]
Ideal model for fitting total bindingK
D = 8 nM k = 7944 K
N ~ O (1 M)
Typ
hoo
n R
eadin
gAptamer No108 Conc (nM)
Surface for TP immobilization
Protein
Aptamer
Biotin
Streptavidin (SA)
Alkaline Phosphatase (AP) Unreacted
Substrate
ReactedSubstrate
Enzyme-Linked Immunosorbent Assay (ELISA)
SA-Biotin interaction Kd ~ 10-15 M = fM ~ ca 260 pNt
Typhoon scan aptamer 5-fold dilution series
Aptamer with nM affinity confirmed
Kd = 8 nM
Combination of Figure Presentation
LC Chen at BIME NTU
Frequently Used Software
bull Word
bull PowerPoint
bull CorelDraw
bull Photoshop
bull Excel
bull Origin
bull Grapher
bull SigmaPlot
bull Othershellip
LC Chen at BIME NTU
LC Chen at BIME NTU
A final and essential step for data presentation consider the efficiency (do things right) and effectiveness (do the right things) Recall the model of ldquoApple Dailyrdquo
If you canrsquot read more papershellip
Can you tell the difference between the figures in a rejected paper and an accepted paper
LC Chen at BIME NTU
同學在論文圖表製作過程中常見違反學術倫理而不自覺的情事
直接剪貼網路( Internet )或文獻( PDF )圖片但未經授權甚至未合理引用 ( cf 原文引用)
製作漂亮數據或使用合成照片以滿足自己的結論
未重複實驗僅報導成功一次的數據
LC Chen at BIME NTU
Essence of Scientific ResearchA valuable thesis is based on the REPRODUCIBLE FACTS AND FIGURES and the most importantly YOUR OWN CREACTIONS NOT NUMBER OF PAGES
httpwwwcartoonstockcomdirectorysscientistasp
LC Chen at BIME NTU
Frequently Used Software
bull Word
bull PowerPoint
bull CorelDraw
bull Photoshop
bull Excel
bull Origin
bull Grapher
bull SigmaPlot
bull Othershellip
LC Chen at BIME NTU
LC Chen at BIME NTU
A final and essential step for data presentation consider the efficiency (do things right) and effectiveness (do the right things) Recall the model of ldquoApple Dailyrdquo
If you canrsquot read more papershellip
Can you tell the difference between the figures in a rejected paper and an accepted paper
LC Chen at BIME NTU
同學在論文圖表製作過程中常見違反學術倫理而不自覺的情事
直接剪貼網路( Internet )或文獻( PDF )圖片但未經授權甚至未合理引用 ( cf 原文引用)
製作漂亮數據或使用合成照片以滿足自己的結論
未重複實驗僅報導成功一次的數據
LC Chen at BIME NTU
Essence of Scientific ResearchA valuable thesis is based on the REPRODUCIBLE FACTS AND FIGURES and the most importantly YOUR OWN CREACTIONS NOT NUMBER OF PAGES
httpwwwcartoonstockcomdirectorysscientistasp
LC Chen at BIME NTU
LC Chen at BIME NTU
A final and essential step for data presentation consider the efficiency (do things right) and effectiveness (do the right things) Recall the model of ldquoApple Dailyrdquo
If you canrsquot read more papershellip
Can you tell the difference between the figures in a rejected paper and an accepted paper
LC Chen at BIME NTU
同學在論文圖表製作過程中常見違反學術倫理而不自覺的情事
直接剪貼網路( Internet )或文獻( PDF )圖片但未經授權甚至未合理引用 ( cf 原文引用)
製作漂亮數據或使用合成照片以滿足自己的結論
未重複實驗僅報導成功一次的數據
LC Chen at BIME NTU
Essence of Scientific ResearchA valuable thesis is based on the REPRODUCIBLE FACTS AND FIGURES and the most importantly YOUR OWN CREACTIONS NOT NUMBER OF PAGES
httpwwwcartoonstockcomdirectorysscientistasp
LC Chen at BIME NTU
A final and essential step for data presentation consider the efficiency (do things right) and effectiveness (do the right things) Recall the model of ldquoApple Dailyrdquo
If you canrsquot read more papershellip
Can you tell the difference between the figures in a rejected paper and an accepted paper
LC Chen at BIME NTU
同學在論文圖表製作過程中常見違反學術倫理而不自覺的情事
直接剪貼網路( Internet )或文獻( PDF )圖片但未經授權甚至未合理引用 ( cf 原文引用)
製作漂亮數據或使用合成照片以滿足自己的結論
未重複實驗僅報導成功一次的數據
LC Chen at BIME NTU
Essence of Scientific ResearchA valuable thesis is based on the REPRODUCIBLE FACTS AND FIGURES and the most importantly YOUR OWN CREACTIONS NOT NUMBER OF PAGES
httpwwwcartoonstockcomdirectorysscientistasp
LC Chen at BIME NTU
同學在論文圖表製作過程中常見違反學術倫理而不自覺的情事
直接剪貼網路( Internet )或文獻( PDF )圖片但未經授權甚至未合理引用 ( cf 原文引用)
製作漂亮數據或使用合成照片以滿足自己的結論
未重複實驗僅報導成功一次的數據
LC Chen at BIME NTU
Essence of Scientific ResearchA valuable thesis is based on the REPRODUCIBLE FACTS AND FIGURES and the most importantly YOUR OWN CREACTIONS NOT NUMBER OF PAGES
httpwwwcartoonstockcomdirectorysscientistasp
LC Chen at BIME NTU
Essence of Scientific ResearchA valuable thesis is based on the REPRODUCIBLE FACTS AND FIGURES and the most importantly YOUR OWN CREACTIONS NOT NUMBER OF PAGES
httpwwwcartoonstockcomdirectorysscientistasp